assays of osteogenic differentiation by cultured human mesenchymal stem cells

Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf

Ngày tải lên : 09/08/2014, 10:23

... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... expression of BMP-2 and chordin during the in vitro osteogenic differentiation of human MSCs was confirmed BMP-2 transcripts were expressed constitutively by MSCs cultured in BM Induction of osteogenic ... Kassem M: Inhibition of osteoblast differentiation but not adipocyte differentiation of mesenchymal stem cells by sera obtained from aged females Bone 2006, 39:181-188 Page of (page number not...

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Ngày tải lên : 09/08/2014, 14:22

... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal ... 6-carboxyfluorescein by the esterases of living cells to label viable cells This combination allows the differentiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5 ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...

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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells

Ngày tải lên : 09/09/2015, 10:07

... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... effectively induce osteogenic differentiation of stem cells without the need for osteogenic solutes The scaffolds were enriched with mesenchymal stem cells (MSCs) to provide the osteogenic property ... bone ECM can enhance the osteogenic differentiation of mesenchymal stem cells and promote bone formation By culturing human MSCs on PLLA electrospun nanofibrous scaffolds in osteogenic (Ost) medium,...

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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx

Ngày tải lên : 10/08/2014, 05:21

... infected cells for 48 hours Results FN and LAM enhance differentiation of MSCs into insulin producing cells To examine the effects of ECM, FN and LAM on IPC differentiation by MSCs, a four-stage differentiation ... vitro differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved ... promote differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal...

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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Ngày tải lên : 11/09/2015, 09:16

... regulate the osteogenic differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation ... osteogenic differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express vascular cell ... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007 ;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche,...

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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1

Ngày tải lên : 09/09/2015, 10:16

... 2.8.2.1 Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem ... Chong, Z Zhang, M Choolani, J Chan Human endothelial progenitor stem cells enhances osteogenic response of bone marrow derived human fetal mesenchymal stem cells through paracrine signalling ... niche: continuous culture of fresh bone marrow aspirates in hypoxia enhances osteogenic differentiation of human fetal mesenchymal stem cells International Society for Stem Cell Research (ISSCR),...

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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot

Ngày tải lên : 07/08/2014, 23:22

... ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu erew ,gk 95.0 ± 77.3 dehgiew ... wef a ,revewoh ;noitamrof ytivac fo egatnecrep tsellams eht detartsnomed puorg PC eht ,noitidda nI spuorg GBCU dna CSM-BCU eht ni noitamrof ytivac fo egatnecrep rellams a dnuof ew ,sllec mets detnalpsnart ... sllec detnalpsnart eht rof troppus reffo yam droc derujni eht ,droc lanips eht fo sdne lamixorp dna latsid eht ot noitcennoc rof ,slairetam ro sllec suoirav fo pu edam dloffacs ro egdirb a htiw...

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Báo cáo khoa học: "Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs" ppsx

Ngày tải lên : 07/08/2014, 23:22

... tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood Stem Cells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors of mesenchymal stem cells recruit ... facilitate proliferation of mononucleated cells from cord blood as verified by fluorescence-activated cell sorting (FACS) analysis, and by the in vitro differentiation of bone [21] Cells (1 × 10 ) were ... radiographs of antebrachium at 0, 4, 8, and 12 weeks after first injection of canine umbilical cord blood derived stem cells was decreased by weeks after injection The dog could bear weight partially by...

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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps

Ngày tải lên : 07/08/2014, 23:22

... increasing the passage number of adult stem cells often leads to a decline in the multipotent abilities [36] Human mesenchymal stem cells could be proliferated and have differentiation abilities ... Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury Spinal Cord 2007, 45, 15-24 12 Hipp J, Atala A Sources of stem cells for ... Chen TH Isolation of multipotent mesenchymal stem cells from umbilical cord blood Blood 2004, 103, 1669-1675 Mauney JR, Volloch V, Kaplan DL Role of adult mesenchymal stem cells in bone tissue...

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Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot

Ngày tải lên : 12/08/2014, 15:23

... cell-mediated mesenchymal stem cell differentiation Stem Cells 2006, 24:707-716 Le Visage C, Kim SW, Tateno K, Sieber AN, Kostuik JP, Leong KW: Interaction of human mesenchymal stem cells with disc cells: ... Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Wuertz K, Godburn K, Neidlinger-Wilke C, Urban J, Iatridis JC: Behavior of mesenchymal stem cells in the chemical ... and progression of chondrogenic differentiation of mesenchymal stromal cells in vitro Stem Cells Dev 2009, 18:881-892 42 Weiss S, Hennig T, Bock R, Steck E, Richter W: Impact of growth factors...

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Establishment of autologous culture systems for human embryonic stem cells

Ngày tải lên : 11/09/2015, 10:00

... Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells ... sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff Bongso, ... types of cells which are in the closely related families (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells...

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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives

Ngày tải lên : 11/09/2015, 10:14

... 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... the study of SHH signaling in hESC offers to augment the current understanding of the role of SHH in humans, that is based largely on the mouse model Figure 2.6 Human embryonic stem cells (hESC) ... mechanisms of neural differentiation 2.12 Neural differentiation of hESC To achieve directed differentiation of neurons, it is essential that there is sufficient knowledge of the differentiation...

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Regulation of gene expression by esrrb in embryonic stem cells

Ngày tải lên : 12/09/2015, 08:19

... Examples of pluripotent stem cells include the embryonic stem (ES) cells, embryonic germ (EG) cells and embryonal carcinoma (EC) cells Multipotent stem cells can give rise to cells of certain ... specialized lineages Many adult stem cells are multipotent, including hematopoietic stem cells, mesenchymal stem cells, and other adult progenitor cells Pluripotent mouse ES cells are derived from the ... the study of mouse ES cells may be applied to improve the understanding of human ES cell biology Due to ethical consideration and limited sources of human embryos, the study of human ES cells is...

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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt

Ngày tải lên : 07/08/2014, 18:21

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc

Ngày tải lên : 30/03/2014, 04:20

... evidence of transdifferentiation by MET in the process of hepatic differentiation of AT-MSCs No previous report has demonstrated evidence of transdifferentiation of committed adult stem cells In ... Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal ... 2, human liver; lane 3, AT-MSC-Hepa; lane 4, human primary hepatocytes The expression level of human hepatocytes was set to 1.0 induced by hepatic differentiation treatment of ATMSCs A number of...

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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt

Ngày tải lên : 10/08/2014, 05:21

... Spontaneous differentiation of germ cells from human embryonic stem cells in vitro Human molecular genetics 2004, 13:727-739 29 Niwa H, Miyazaki J, Smith AG: Quantitative expression of Oct-3/4 defines differentiation, ... 11 of 13 Figure The expression profile of 3A6 immortalized hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem ... RT-PCR analysis of expression levels of hTERT and GAPDH, and (B) Real-time RT-PCR analysis of expression levels of DNMT1, DNMT3A and DNMT3B in primary human mesenchymal stem cells transfected...

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Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf

Ngày tải lên : 10/08/2014, 05:21

... cardiomyocytes sheets from differentiated human umbilical cord mesenchymal stem cells We assessed viability of the cells of sheets at room temperature No indication of cell apoptosis was noticed from ... Review: Human Umbilical Cord Stroma with Regard to the Source of Fetus-Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem ... maintaining the multipotency of different human stem cells Our investigation indicates that S1P promotes differentiation of HUMSCs towards cardiomyocytes and functionally maturation of hUC-MSCs derived...

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Báo cáo y học: "HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells" pot

Ngày tải lên : 13/08/2014, 01:20

... evaluate adipogenic potential of amniotic fluid-derived human mesenchymal stem cells Stem Cells Dev 2006, 15:719-726 55 Montaln H, Schichor C, Lah TT: Human mesenchymal stem cells and their use in cell-based ... of human bone marrow stromal fibroblasts Blood 1990, 76:317-322 40 Wang L, Mondal D, La Russa VF, Agrawal KC: Suppression of clonogenic potential of human bone marrow mesenchymal stem cells by ... induce adipogenic differentiation, confluent cells were cultured as follows: three cycles of days induction medium and days maintenance medium of hMSC Mesenchymal Stem Cell Adipogenic Differentiation...

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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

Ngày tải lên : 18/02/2014, 04:20

... DR (1999) Multilineage potential of adult human mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population ... Kitamura S & Nagaya N (2007) Effect of hypoxia on gene expression of bone marrowderived mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan ... antiﬁbrotic effect of transplanted mesenchymal stem cells in a rat model of global heart failure Mol Biol Rep 36, 725–731 Li L, Zhang Y, Li Y, Yu B, Xu Y, Zhao S & Guan Z (2008) Mesenchymal stem cell...

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Báo cáo Y học: Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine pdf

Ngày tải lên : 17/03/2014, 23:20

... Addition of Z-VAD.FMK to DENSPM-treated cells decreased the percentage of cells in the sub-G1 region to Fig The eﬀect of DENSPM treatment on the polyamine content of L56Br-C1 cells Cells were ... The percentage of cells in the sub-G1 region as a measure of apoptotic cells The L56Br-C1 cells were seeded in the absence or presence of 10 lM DENSPM with or without the addition of the general ... air The growth of the cells was monitored at each passage by counting in a haemocytometer and the cells were regularly grown without antibiotics to exclude cryptic infections Cells were thawed...

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