aspergillus ustus and aspergillus terreus
Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... both XBS and XAN involved in substrate interaction were selected based on the crystal structures of XBS and XAN soaked with xylotetraose and xylopentaose, respectively [12] (Fig 1), and were ... = 9.76 · 10)10 M and AzU = 50.8 · 10)10 M and for the wild-type XAN, X6U = 1.02 · 10)10 M, XyU = 7.72 · 10)10 M and AzU = 9.65 · 10)10 M for the activity on X6, Xylazyme AX and Azo-wheat AX, ... xylanases and other glycoside hydrolases will need to be determined Materials and methods Materials All chemicals, solvents and reagents were purchased from Sigma-Aldrich (Bornem, Belgium) and are...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... A.G.J (1991) Purification and characterization of endo-1,4-b-D-galactanases from Aspergillus niger and Aspergillus aculeatus: Use in combination with arabinases from Aspergillus niger in enzymic ... plates and purified by rescreening at low plaque density Standard methods were used for other DNA manipulations, such as Southern analysis, subcloning, DNA digestions, and lambda phage and plasmid ... D-galactose, D-galactobiose and D-galactotetraose were used as standards to identify the D-galactose and D-galacto-oligosaccharides The calculated areas for D-galactose and D-galacto-oligosaccharides...
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Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt
... residues of the proteins, His50, Glu96 and His137 in a-sarcin and His36, Glu82, and His123 in a-sarcin D(7–22) Images were generated by the MOLMOL program [30] and subsequently rendered with MegaPov ... proteins and characterized from structural and enzymatic points of view The immunoreactivity of these two proteins has also been studied Results Production, isolation, and spectroscopic and structural ... Easton, MD, USA) at 50 nmÆmin)1 scanning speed; 0.1- and 1.0-cm opticalpath cells, and 0.1 and 0.5 mgÆmL)1 protein concentration were used in the far- and near-UV, respectively Mean residue weight ellipticities...
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Báo cáo khoa học: Relationships between the ethanol utilization (alc ) pathway and unrelated catabolic pathways in Aspergillus nidulans pptx
... amino acids, putrescine, c-aminobutyric acid (GABA) and small carboxylic esters Materials and methods Strains, media and growth conditions Aspergillus nidulans strains used in this study are ... Catabolism of L-proline and L-arginine does not lead to induction of the alc genes L-Proline and L-arginine can serve as sole carbon and nitrogen sources for A nidulans and both are catabolized ... conditions, RNA isolation and Northern blots were as described in Materials and methods Membranes were hybridized with 32P-labelled probes specific for the alcA, alcR, facA, facB and c-actin (acnA) genes...
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Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf
... using a PCR cloning approach, and the cloned PCR products were checked by sequencing Table shows the primers, vectors, and restriction sites used in the cloning strategy, and Table lists the primer ... name of the primers used for amplification of the laccase cDNA and addition of cloning sites, recipient Aspergillus expression vector and restriction sites used in the final cloning procedure Primers ... containing formamide and formaldehyde [22] and loaded on a 1% Tris/acetate/EDTA agarose gel containing 6% formaldehyde [22] After electrophoresis, RNA was blotted onto Hybond N+ and UV crosslinked...
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Báo cáo khoa học: Methylcitrate synthase from Aspergillus fumigatus Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia ppt
... produced and sequenced (Accession No AJ888885) Comparison of genomic and cDNA revealed two introns with a size of 58 and 64 bp Removal of the introns led to an open reading frame of 465 amino acids and ... wild-type strain ATCC46645 and a methylcitrate synthase deletion strain were point inoculated on each plate and incubated for 72 h at 37 °C and photographed Synthesis of cDNA and sequence analysis ... antifungals such as amphotericin B and itraconazol, which have severe side-effects [10–13] In order to identify new targets for drug development and to understand the impact of specific fungal genes...
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Báo cáo khoa học: The isopenicillin N acyltransferases of Aspergillus nidulans and Penicillium chrysogenum differ in their ability to maintain the 40-kDa ab heterodimer in an undissociated form pdf
... nidulans Lanes 1, 2, and 9, E coli [pPBCab], contains the gene penDE from P chrysogenum (lanes and without IPTG induction and lanes and with 0.5 mM IPTG) Lanes 3, 4, 10 and 11, E coli [pULCTab], ... nidulans (lanes and 10 without IPTG induction and lanes and 11 with 0.5 mM IPTG) Lane 5, molecular mass markers Lanes and 7, E coli [pT7.7] used as control (lane without IPTG induction and lane with ... to the 29-kDa and 11-kDa subunits) in lane 4; lane 5, molecular mass markers; lanes and 7, control E coli [pT7-7] without and with induction; lanes and 9, E coli [pPBCab] without and with induction;...
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Báo cáo hóa học: " Biofabrication of Anisotropic Gold Nanotriangles Using Extract of Endophytic Aspergillus clavatus as a Dual Functional Reductant and Stabilizer" potx
... carbon-coated copper grid, and the solution was allowed to stand for min, then excess solution was removed carefully, and the grid was allowed to dry for an additional min; the average size and size distributions ... growth and nucleation of nanoparticles [25,26] However, the biochemical mechanism of metal ion reduction and the subsequent NP formation remain unexplored and need further research Thus, thorough and ... The surface plasmon bands for the gold nanoparticles usually ranges between 510 and 560 nm in aqueous solution depending upon the function of their morphology, since plasmon bands are very sensitive...
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Báo cáo nghiên cứu khoa học: " CHARACTERIZATION OF PROTEASE FROM ASPERGILLUS ORYZAE SURFACE CULTURE AND APPLICATION IN FISH SAUCE PROCESSING" pps
... ninhydrin reagent RESULTS AND DISCUSSION Our prelimenary study shows that the optimal pH and temperature of the protease produced by Aspergillus oryzae surface culture are 7.0 and 50oC respectively ... was 65.45% and the purified degree was 3.03 This purified protease was used for determination of Km, Vmax and applied in fish sauce processing 2.3 Determination of kinetic parameters Km and Vmax ... with NaCl 50g/L and 100g/L, the enzyme activity remained 70% and 50% in comparison with the control sample.[2] 3.1 Influence of different ions to the protease activity Figures and show the influence...
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Báo cáo y học: "Aspergillus antigen induces robust Th2 cytokine production, inflammation, airway hyperreactivity and fibrosis in the absence of MCP-1 or CCR2" pptx
... production, AHR and fibrosis Methods Mice Breeding pairs of Mcp-1+/+ and Mcp-1-/- mice [19] and Ccr2+/+ and Ccr2-/- mice [21] were generated as previously described Mice were bred and maintained ... MCP-1- and CCR2-deficient mice developed marked airway inflammation in response to Aspergillus antigen (Figs 2C and 2D) The BAL fluid cell counts from Aspergillus antigen-treated MCP-1- and CCR2-deficient ... in Aspergillus- antigen-treated Mcp-1-/- and Ccr2-/- mice than in Aspergillusantigen-treated wild-type mice, this trend was not statistically significant and was not observed in two additional Aspergillus- antigen-challenge...
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Báo cáo khoa học: "Isolation of Aspergillus spp. from the respiratory tract in critically ill patients: risk factors, clinical presentation and outcome" potx
... isolation of an Aspergillus sp and suggestive clinical or radiological findings; 'possible' aspergillosis requires the presence of risk factors in the host and isolation of an Aspergillus sp., ... respiratory isolation of Aspergillus spp.; to assess clinical features, treatment and outcomes in patients with Aspergillus spp recovered from respiratory secretions; and to evaluate the correlation ... comparison of risk factors for the isolation of Aspergillus spp between groups of patients with Aspergillus spp., patients with Candida spp infection, and noncolonized, uninfected patients was conducted...
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Báo cáo y học: "Aspergillus fumigatus allergen expression is coordinately regulated in response to hydrogen peroxide and cyclic AMp" doc
... proteases) and proteins of unknown to date functions (Asp f 2, -4, -7 and -17) Asp f 11, -12 and -22 were induced 5-10 fold under the same conditions Expression of Asp f (peroxiredoxin) and Asp ... MOI - 1:200 and 1:2000 spores/macrophage The MOI used were chosen to give conditions where both fungus and macrophage were able to grow and remain viable for Fraczek et al Clinical and Molecular ... complex and clearly lacks the coordinated nature of the responses observed in axenic culture (Figure 4) The protease allergens, Asp f 5, -10 and -13 and -18 were expressed at very low levels and...
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tóm tắt luận án isolation and screening of aspergillus niger biosynthesize high activity pectin methylesterase
... selected isolates T1, N1, So2 and R1 had 99 ÷ 100% identity to Aspergillus niger The optimal conditions of pectin methylesterase biosynthesis from Aspergillus niger So2 and R1 in solid-state fermentation ... state fermentation (SSF) of isolated and selected Aspergillus niger from the practical conditions of Vietnam Sixty species which have morphologically similar Aspergillus niger were isolated from ... “Isolation and screening of Aspergillus niger biosynthesize high activity pectin methylesterase” was performed in the laboratory of Food Technology Department, College of Agriculture and Applied...
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purification and characterizations of phytase enzyme form aspergillus niger
... phytase: purification and characterization Biotechnol 19:508-520 Ullah, A H J and Gibson, D M 1987 Extracellular phytase (E.C 3.1.3.8) from Aspergillus ficuum NRRL 3135: Purification and characterization ... about 1-3% of the seeds of grains and legumes, and 60-80% of the total amount of plant phosphorus (Nelson, 1967) Phosphorus is essential for feeding animals, poultry and even for human diet It is ... research, the thesis on "Purification and characterizations of phytase enzyme from Aspergillus niger" has been performed with the aim to obtain purifed phytase and some its valuable characteristics...
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Nghiên cứu một số đặc tính của emzyme β-fructofuranosidase (β-ffase) từ nấm mốc aspergillus
... Development, Vol 9, No.6- 2006 Aspergillus sydowi A5 Phân lập từ tinh bột bắp Aspergillus sydowi A6 Phân lập từ bột sò Aspergillus foetidus A7 Phân lập từ tinh bột bắp Aspergillus foetidus A8 Phân ... Martonez-Fleites,1 and Lazaro Hernandez., Three Acidic Residues Are at the Active Site of a Propeller Architecture in Glycoside Hydrolase Families 32, 43, 62, and 68, Proteins: Structure, Funtion, and Bioinformatics, ... Science, Vol.6, p 952-954 ,2003 [3] Mirza Ahsen Baig, Kiran Shafiq, Sikander Ali, and Shazia Mirza, Effect of Nitrogen and Phosphate Sources on the Biosynthesis of β- fructofuranosidase, Online...
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Aspergillus Flavus và A. parasiticus sinh độc tố Aflatoxin
... Genus :Aspergillus Genus :Aspergillus Species: A flavus Species: A parasiticus Hình 3: Aspergillus flavus A.parasiticus thực phẩm và môi trường nuôi cấy Nguồn gốc Aspergillus flavus Aspergillus ... giai đoạn - Tác động qua lại với AND và ức chế các polymeraza chịu trách nhiệm tổng hợp AND và ARN - Ngừng tổng hợp AND - Giảm tổng hợp AND và ức chế tổng hợp ARN truyền ... từ nấm Aspergillus flavus, parasiticus fumigatus, có nguồn gốc từ lạc khô mốc Nguồn gốc Aflatoxin sản phẩm thứ cấp tiết trình sinh trưởng phát tiển số loài nấm Aspergillus, thường thấy Aspergillus...
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Nghiên cứu khả năng sinh Aspergillus niger pectinmethylesterase trên cơ chất bã táo
... Nguồn giàu enzyme pectinase nấm mốc, nấm men vi khuẩn Nấm mốc: Aspergillus niger, Aspergillus awamori, Aspergillus terrus, Aspergillus saitoi, Penicillium glaucum, Penicillium chrysogenum, ... Production and Applications ISBN 3–527–29592–5 Adams, B and W Kirk (1991) Process for enzyme peeling of fresh citrus fruit US Patent 000 967 Afifi, A F., E M Fawzi., and M A Foaad (2002) Purification and ... of current and potential applications World Journal of Microbiology and Biotechnology, Volume 14, Number 5, October 1998 , Pp: 611–619(9) Brett, C T and K Waldron (1996) Physiology and biochemistry...
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Tuyển chọn nuôi cấy chủng aspergillus awamori sinh tổng hợp endo-β-1, 4-glucanase và đánh giá tính chất lý hóa của endo-β-1, 4-glucanase
... thuộc chi Aspergillus, Trichoderma, Penicillium, Phanerochaete đƣợc nghiên cứu có khả sinh tổng hợp endo-β-1,4-glucanase mạnh nhƣ: Aspergillus niger [25], [26], A flavus, A fumigatus [27] A terreus ... “Tuyển chọn, nuôi cấy chủng Aspergillus awamori sinh tổng hợp endo-β-1,4-glucanase đánh giá tính chất lý hóa endo-β-1,4glucanase” với mục tiêu: (1) Tuyển chọn chủng Aspergillus awamori sinh tổng ... lƣợng phân tử 83 kDa 50 kDa [25], từ A terreus 25 kDa [29], từ Trichoderma reesei 48 kDa 55 kDa [40], [47], từ Bacillus sp D04 có khối lƣợng 35 kDa [56] Sandgren cs (2003) có nghiên cứu chi tiết...
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