apos scid adapted apos marv model to assess the efficacy of potential therapeutics for marv
Báo cáo sinh học: " Genetic characterisation of the recent foot-and-mouth disease virus subtype A/IRN/2005" pptx
... propagation of the virus Amino acid comparison of the Leader protease Figure displays the alignment of the deduced amino acid sequence of the first 96 residues of the FMDV Leader protease from the A/IRN/05 ... alignment of the deduced amino acid sequences of the immuno-dominant residues of the VP1 surface protein, including the GH-loop The alignment consist only of isolates belonging to the FMDV A/IRN/ ... Figure GH-loop of the deduced amino acid sequences of the immuno-dominant residues of the VP1 surface protein, including the Alignment Alignment of the deduced amino acid sequences of the immuno-dominant...
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Báo cáo hóa học: " Genetic characterisation of the recent foot-and-mouth disease virus subtype A/IRN/2005" ppt
... propagation of the virus Amino acid comparison of the Leader protease Figure displays the alignment of the deduced amino acid sequence of the first 96 residues of the FMDV Leader protease from the A/IRN/05 ... alignment of the deduced amino acid sequences of the immuno-dominant residues of the VP1 surface protein, including the GH-loop The alignment consist only of isolates belonging to the FMDV A/IRN/ ... Figure GH-loop of the deduced amino acid sequences of the immuno-dominant residues of the VP1 surface protein, including the Alignment Alignment of the deduced amino acid sequences of the immuno-dominant...
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Báo cáo y học: " Dideoxynucleoside HIV reverse transcriptase inhibitors and drug-related hepatotoxicity: a case report" pot
... the importance of d4T as a possible causative agent of hepatotoxicity, even in the absence of other signs of mitochondrial toxicity Liver biopsy may provide further important information regarding ... with d4T and other ddX use [11,12] A steatotic liver, particularly if some grade of fibrosis is present, may be more susceptible to the toxic effect of the drugs Therefore, use of PI boosted ... conceiving the study, in the acquisition of data and revised the manuscript LB and MG analyzed the liver biopsy and revised the manuscript CT conceived the study and participated in the drafting of the...
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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
... observed in the presence of different concentrations of the rst inhibitor, in the absence or contemporaneous presence of the second inhibitor, leads to a series of lines that are parallel if the two ... inhibitors compete for the same binding site, whereas they intersect if the inhibitors bind to different enzyme sites [25] Therefore, the HIV-1 RT RNase H activity was measured in the presence of ... RT inhibitors F Esposito et al for the RDDP inhibition activity In the case of KNA53, the bulkier moiety 1-4-bromophenyl)-2-oxyethanone does not allow the compound to enter further into the cavity...
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Báo cáo Y học: The processivity and fidelity of DNA synthesis exhibited by the reverse transcriptase of bovine leukemia virus pot
... of the processivity and the fidelity of DNA synthesis (namely, the ability of BLV RT to misincorporate nucleotides at the 3¢ end of the growing DNA strand and the further extending of the preformed ... and the variations were usually < 15% The relative frequency Fext values are the ratio of the rate constants (Vmax/Km) for the mispair divided by the ratio of the corresponding constants for the ... [32,33] Therefore, using parameters of both the capacity to misincorporate an incorrect nucleotide and the ability to extend the preformed 3¢ mispairs, it was suggested that the overall rates of the...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... 15 with the genotypes present in the stool panel (Fig 3) The lack of cross reactivity allows these probes to be left on the membrane and as they are designed to anneal to PCR products at the same ... denaturation step of 94°C for followed by 45 cycles of 94°C for s, 52°C for 10 s, 72°C for 17 s and a fluorescent read step of 84°C for 10 s to melt primer dimers For the creation of standard curves, ... denaturation step of 94°C for followed by 40 cycles at 94°C for 10 s, 45°C for 10 s 72°C for 15 s and a fluorescent read step of 85°C for 15 s to melt primer dimers The reaction was performed using...
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Báo cáo sinh học: " Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" pdf
... times prior to RTPCR At the end of the incubation, the thermocycler was programmed to ramp the samples at the maximum rate (2.2°C/sec) to 55°C and hold at this temperature for prior to the 50°C, ... [13] At the end of the UNG incubation, the samples were held at 55°C to rapidly inactivate UNG just prior to the RT step which was 50°C for 30 For the RT-PCR reagents used (as well as many other ... increasing the CT for RNA detection It is interesting to note that, based on analysis of the standard curves, increases observed in the CT for RNA were due both to the presence of UNG and the incubation...
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Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
... 15 with the genotypes present in the stool panel (Fig 3) The lack of cross reactivity allows these probes to be left on the membrane and as they are designed to anneal to PCR products at the same ... denaturation step of 94°C for followed by 45 cycles of 94°C for s, 52°C for 10 s, 72°C for 17 s and a fluorescent read step of 84°C for 10 s to melt primer dimers For the creation of standard curves, ... denaturation step of 94°C for followed by 40 cycles at 94°C for 10 s, 45°C for 10 s 72°C for 15 s and a fluorescent read step of 85°C for 15 s to melt primer dimers The reaction was performed using...
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Báo cáo hóa học: " Sargassum fusiforme fraction is a potent and specific inhibitor of HIV-1 fusion and reverse transcriptase" pot
... (NL4-3) at multiplicity of infection (moi) of 0.01 for 1.5 h, washed times, and returned to culture with the same concentration of each treatment, for the duration of the experiment (A) On day ... R5-tropic HIV-1 S fusiforme inhibits HIV-1 fusion by blocking CD4 receptor Viral entry into cells consists of two distinct steps of 1) virus binding to the cellular receptor and coreceptor, which is ... HIV-1 binding to the cell surface receptor but not fusion or entry Except for h SP4-2 pretreatment of cells that was done at 37°C to allow for SP4-2-CD4 interaction, we performed all the subsequent...
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báo cáo hóa học:" Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" ppt
... times prior to RTPCR At the end of the incubation, the thermocycler was programmed to ramp the samples at the maximum rate (2.2°C/sec) to 55°C and hold at this temperature for prior to the 50°C, ... [13] At the end of the UNG incubation, the samples were held at 55°C to rapidly inactivate UNG just prior to the RT step which was 50°C for 30 For the RT-PCR reagents used (as well as many other ... increasing the CT for RNA detection It is interesting to note that, based on analysis of the standard curves, increases observed in the CT for RNA were due both to the presence of UNG and the incubation...
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báo cáo hóa học:" Substitutions in the Reverse Transcriptase and Protease Genes of HIV-1 Subtype B in Untreated Individuals and Patients Treated With Antiretroviral Drugs" potx
... Inc (Toronto, Ontario, Canada) The sequencing of RT was limited to positions 38249 due to the type of assay performed Sequencing of both the RT and PR genes was also employed to determine the ... cellular factor, APOBEC3G, a cytidine deaminase that converts cytosine to uracil The activity of APOBEC3G is inhibited by the Vif protein.[5,7,8] In the absence of Vif, the synthesis of the negative ... synthesis of the negative strand of DNA can result in the insertion of a uracil as a result of the deamination of a cytosine, leading to the inclusion of an adenosine instead of guanosine in positive-stranded...
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Báo cáo khoa học: "Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas" pptx
... citsalpanA suotamommasP lanoitisnarT citsalborbiF lailehtognineM lailehtognineM lailehtognineM lailehtognineM lailehtognineM lailehtognineM lailehtognineM 21 21 21 21 51 61 21 9 21 11 11 01 01 01 71 ... esadixorep -nitoib-nidivatperts a yb dewollof ,Co4 ta thginrevo la te iloirdnaM anaicuL 211 amoigninem rehto gnoma ,taht ,epytotsih citsalpana eht ni depuorg erew eseht fo eerht dna ,epytonehp evissergga ... seires ruo ni evitceffeni saw gniniatsonummi 1BIM ,ylbaecitoN snoitamrofsnart laromut rieht ni detcepxe eb tonnac ytivitca citotim hgih a erofereht dna ,]2[ noisivid llec fo etar wols a evah ,esira...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
... simultaneously for the messages of both target gene and β-actin gene in the same sample tube when the intensity of the reporter fluorescent signal reached 10 times the standard deviation of the baseline of ... isoforms were detected in all synovial samples The expression of the messages for HAS-1 and HAS-2 was significantly less in the synovium of OA than in the control synovium (83% and 48% of the ... to all the members of the Department of Orthopaedic Surgery, The Jikei University School of Medicine, for the collection of samples 23 24 References R519 Saari H, Kontinen YT: Determination of...
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Báo cáo y học: ": Prevalence of reverse transcriptase and protease mutations associated with antiretroviral drug resistance among drug-naïve HIV-1 infected pregnant women in Kagera and Kilimanjaro regions, Tanzania" pptx
... respectively, reflects the limited use of antiretroviral drugs in the two regions to date The absence of TAM among the strains investigated may indicate the low use of the AZT-based therapy in the population ... was performed based on the national HIV testing algorithm using the two rapid assay confirmatory strategy for the detection of antibodies to HIV [17] HIV antibody testing was performed at the clinics ... due to a low level of exposure of HIV infected individuals in these regions to antiretroviral drugs The results of this study need to be confirmed by investigations in different regions of the...
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Báo cáo y học: " Efficacy, safety and pharmacokinetic of once-daily boosted saquinavir (1500/100 mg) together with 2 nucleos(t)ide reverse transcriptase inhibitors in real life: a multicentre prospective study" ppt
... maintenance therapy [23,24] We are aware that the open-label characteristics of the study, the heterogeneity of the analyzed population and the lack of available genotypic resistance tests in some of the ... “simplification” group may be the cause of the higher efficacy observed in these patients Although the efficacy of a once daily dosing of SQV/r has mainly been shown to be effective in an Asian ... with fewer pills, although the experience with this dosage is still very scarce [12] The aim of the present study was to assess the efficacy, safety and pharmacokinetics of once-daily SQVr 1500/100...
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Báo cáo y học: " A Leu to Ile but not Leu to Val change at HIV-1 reverse transcriptase codon 74 in the background of K65R mutation leads to an increased processivity of K65R+L74I enzyme and a replication competent virus" pptx
... 30 W for h The wet gels were exposed to autoradiography for 30 to hr To determine relative density of bands in the gel, we scanned group of bands using Bio Image Intelligent Quantifier ® software ... related to the observed attenuation of the virus in the presence of these mutations [5,7,12,15] Comparison of replication kinetics of mutant viruses in MT-2 cells Since the presence of higher ... coordinating the incoming triphosphate In the process, Arg72 donates hydrogen bonds to the a-phosphate and the ε-amino group of Lys65 donates hydrogen bonds to the g-phosphate These events lead to the...
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Báo cáo y học: " A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses?" docx
... order to examine the assay specificity [6-8] Detection of the mimic samples by the duplex real-time RT-PCR The mimic samples were prepared by mixing the virus with the mouse brain tissue, then the ... with the single system In order to improve the sensitivity of the duplex system, we further optimized the concentrations of the primers and probes for EEEV First, the assay was conducted with different ... http://www.virologyj.com/content/7/1/284 Page of Figure Analytical sensitivity of the duplex real time RT-PCR assay based on the 10-fold dilution series of the in vitro-transcribed RNA The amplification blot for EEEV(A) and the associated...
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Báo cáo y học: " The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro" doc
... reported by Lemay et al to directly interact with the RNase H domain of HIV-1 RT [9] To further define the structural determinants of the HuR-RT interaction and to elucidate the mechanism(s) by which ... substrate The sequences of the T/P is indicated with the ARE in bold and underlined Binding of NusA-HuR to this T/P is observed (left-hand autoradiograph) However, the efficiency of HIV-1 RT DNA synthesis ... confirmed the interaction by a homogenous time-resolved fluorescence binding assay The authors mapped the HIV-1 RT-HuR binding sites to the RNase H domain of RT and to the C-terminus of HuR (see...
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Báo cáo y học: "Comparative biochemical analysis of recombinant reverse transcriptase enzymes of HIV-1 subtype B and subtype " ppt
... enzymes to their active triphosphate forms (diphosphate for tenofovir), which bind to the active site of RT, acting as competitive inhibitors of RT and interfering with the addition of incoming ... run in the absence of trap to ensure that similar amounts of enzyme activities were present in the reactions The sizes of some fragments of the standard are indicated on the left side of the panel ... a molar ratio of 1:1, denatured at 85°C for min, and then sequentially cooled to 55°C for and 37°C for to allow for specific annealing of primer to the template Reactions were performed as above...
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Báo cáo y học: "The evolution of HIV-1 reverse transcriptase in route to acquisition of Q151M multi-drug resistance is complex and involves mutations in multiple domains" ppt
... account for nearly 90% of the epidemic worldwide [37] In this study, we took advantage of treatment failure in the absence of viral load-guided therapy to dissect the relative contribution of RT ... initiation of therapy, even though the viral load had been relatively high at earlier time points This raises the possibility of suboptimal use of the drugs contributing to the emergence of the Q151M ... amounts of virus The patient’s sample before initiation of therapy was not available, thus the replicative capacity of the viruses measured by relative luciferase light units was compared to that of...
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