antiprogestin onapristone in breast cancer cells using microarray analysis
Detection of breast cancer cells using targeted magnetic nanoparticles and ultra-sensitive magnetic field sensors potx
... added in order to label actin filaments After washing in PBS, cells were incubated in 4’,6-diamidino-2-phenylindole (DAPI) or Topro-3 (Invitrogen Corporation) to counterstain nuclei and were inverted ... cells (2.75 × 106), MCF7 breast cancer cells (0.18 × 106), and MDA-MB-231 breast cancer cells (0.11 × 106) The Chinese hamster ovary (non -breast) cell line has a very low number of Her2-binding ... of Her2 expression on breast cancer cell lines We examined several breast cancer cell lines to identify cells that expressed Her2 The number of Her2-binding sites was determined by flow cytometry...
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Báo cáo Y học: Progestin upregulates G-protein-coupled receptor 30 in breast cancer cells pot
... expressed in MPA-treated cells, but not in the control cells at either 24 or 48 h in differential display analysis Progestins upregulate GPR30 mRNA in MCF-7 breast cancer cells Northern-blot analysis ... progestin administration was thus associated with the beginning of growth inhibition by progestins GPR30 mRNA is regulated according to the dose of progestin, which also correlated with progestin-induced ... beginning of progestin-induced growth inhibition MCF-7 cells were treated with MPA for 2–24 h and RNA was extracted for Northern blotting to analyse the mRNA Cell proliferation was measured using...
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Báo cáo khoa học: BRCA1 accumulates in the nucleus in response to hypoxia and TRAIL and enhances TRAIL-induced apoptosis in breast cancer cells pdf
... level in normoxic cells There was no change in the steady-state level of Fig Hypoxia induces nuclear accumulation of BRCA1 in breast cancer cells but not in nonmalignant cells MDA-MB-468 cells, ... BRCA1 induced by hypoxia in MDA-MB-468 cells (Fig 2B), resulting in a 35% decrease in nuclear BRCA1 relative to the level in normoxic cells in the presence of 2E5 The decrease in C Fig Hypoxia-induced ... the data in Fig support the involvement of a TRAIL-dependent process in mediating hypoxiainduced changes in BRCA1 localization TRAIL, but not hypoxia, induces apoptosis in breast cancer cells As...
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Heregulin enhanced tyrosine phosphorylation in breast cancer cells role of PP2A in HER 2 eu oncogenic signalling
... frequent cancers affecting Singapore women, 2003-2007 Breast cancer is the top cancer type affecting the women in Singapore Data extracted from Singapore Cancer Registry, Interim Report: Trends in Cancer ... XVI Chapter Introduction CHAPTER INTRODUCTION 1.1 Breast cancer 1.1.1 Incidence of breast cancer Breast cancer is the second leading cause of cancer deaths in women today after lung cancer and ... ligand-binding domain with two cysteine-rich regions, a hydrophobic short transmembrane domain, and an intracellular domain that contains a catalytic tyrosine kinase domain and a carboxyl terminal...
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ANTI CANCER EFFECTS OF THYMOQUINONE IN BREAST CANCER CELLS INVOLVEMENT OF NON HOMOLOGOUS END JOINING AND TELOMERE TELOMERASE HOMEOSTASIS
... breast cancer cells 37 3.1.1 Breast cancer cells are sensitive to the anti-proliferative effects of TQ 37 3.1.2 TQ causes deficiencies in cell cycle checkpoint function in breast cancer cells ... cells 41 3.1.3 Changes in cell cycle protein expressions in TQ-treated breast cancer cells 44 3.2 DNA damaging effects of TQ in normal and breast cancer cells 47 3.2.1 TQ induces significantly ... Specific protein complexes such as protection of telomeres-1 (POT1), telomeric repeat-binding factor-1 (TRF1), TRF2, TRF1-interacting protein-1 (TIN1), TIN2, TINF2-interacting protein (TPP1) and...
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EFFECTS OF INHIBITING THE MAMMALIAN TARGET OF RAPAMYCIN (MTOR) PATHWAY AND TELOMERASE IN BREAST CANCER CELLS
... telomerase in initiating, regulating and driving cancer phenotypes, including those of breast cancer It also shows that there is limited efficacy of rapamycin as an anticancer agent in both preclinical ... FK506-binding protein 12 (FKBP12) and directly binds to and inhibits TOR proteins through an allosteric site N-terminal to its kinase domain Called the FKBP12-rapamycin binding (FRB) domain, this ... telomerase 24 1.4 BREAST CANCER AS A MODEL OF STUDY .25 1.4.1 mTOR in breast cancer 25 1.4.2 Telomerase in breast cancer 26 1.4.3 Rapamycin in breast cancer .27...
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Investigating functions of ERp29 in mesenchymal to epithelial transition (MET) and epithelial plasticity in breast cancer cells
... protein kinase C Adenosine triphosphate Basic helix-loop-helix Binding protein Bovine serum albumin Cyclin-dependent kinase inhibitor 2B Cytokeratin-19 Cytoplasmic lipid droplets 4’,6-diamidino- ... protein-29 (ERp29) is a chaperone protein that functions in the unfolding and escort of secretory proteins Like other reticuloplasmins, ERp29 is believed to be involved in carcinogenesis In breast ... occludens x Chapter 1: Introduction Chapter 1: Introduction 1.1 Breast cancer 1.1.1 Incidence of breast cancer Each year cancer is claiming millions of lives and affecting millions more In developed country...
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báo cáo khoa học: " Microarray-based analysis of microRNA expression in breast cancer stem cells" doc
... drug-metabolising enzyme CYP1B1 in cancerous tissues [14] Finally, as a tumor suppressor in breast cancer cells, miR-17-5p regulates breast cancer cell proliferation by inhibiting the translation ... staining and immunohistochemical staining The tumors in the BCSCs group were positive for both EMA and SMA, indicating that they were included of both luminal epithelial and myoepithelial cells ... cell line Methods Fluorescence-activated cell sorting (FACS) of BCSCs The human breast cancer cell line MCF-7 was cultured in minimal essential medium (MEM) (Invitrogen, America) Cells in log...
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báo cáo khoa học: "Identification of circulating tumour cells in early stage breast cancer patients using multi marker immunobead RT-PCR" doc
... markers of disseminated breast cancer cells Endocr Relat Cancer 2006, 13:1033-1067 Slade MJ, Coombes RC: The clinical significance of disseminated tumor cells in breast cancer Nat Clin Pract Oncol ... detection of breast cancer cells Breast Cancer Res Treat 2006, 97:41-47 Zach O, Lutz D: Mammaglobin remains a useful marker for the detection of breast cancer cells in peripheral blood J Clin Oncol ... Molecular Detection of Circulating Tumor Cells Using a Multimarker Reverse Transcription-PCR Assay for Cytokeratin 19, Mammaglobin A, and HER2 in Early Breast Cancer Clin Cancer Res 2008, 14:2593-2600...
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Báo cáo Y học: Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine pdf
... between polyamine analogue-induced increases in spermidine/spermine N1-acetyltransferase activity, polyamine pool depletion, and growth inhibition in human melanoma cell lines Cancer Res 51, ... extensively induce cell death in the human breast cancer cell line L56Br-C1 This cell line carries a germ-line mutation (1806 C fi T) in the BRCA1 tumour Fig Propidium iodide-stained nuclei of L56Br-C1 cells ... line was maintained in serial passages in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 10 lgÆmL)1 insulin, 20 ngÆmL)1 epidermal growth factor, nonessential amino...
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báo cáo khoa học: " Salivary a-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells" docx
... predominantly comprised mammary epithelial cells This was additionally determined by immunofluorescence analysis using cytokeratin as a marker protein The mean proportion of cytokeratin-positive cells ... effects in primary rat mammary epithelial cells and human breast tumor cells On the one hand the effects on healthy rat breast cells indicate that endogenous a-amylase might be involved in the ... trypsin For cell counting and passaging, trypsin/EDTA (0.15%) was used to detach cells, and its reaction was stopped with fetal calf serum (20%) in DMEM/F12 Remaining passage (P0) -cells were allowed...
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Báo cáo y học: "Segmentation-based detection of allelic imbalance and loss-of-heterozygosity in cancer cells using whole genome SNP arrays" ppsx
... cell content from simulated data using segmentation Tumor cells (%) Tumor cells using hemizygous loss (%)* Tumor cells using single copy gain (%)† Tumor cells using copy neutral LOH (%)‡ Paired ... certain assumptions For example, by assuming that a certain allelic imbalance occurs in all tumor cells, normal cell contamination becomes the sole driving force behind BAF compression In this ... described in Additional data file Detection of homozygous deletions using the B allele frequency alone can be challenging [22] In the case of complete homozygous deletion in all investigated cells...
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Tài liệu Guidance on Cancer Services Improving Outcomes in Breast Cancer pdf
... Clinical breast examination in primary care Each primary care team should include at least one practitioner who has had specific training in carrying out clinical breast examination (CBE) in ... to breast clinics for investigation of possible or suspected breast cancer are referred by GPs; the remaining 20% are identified by routine screening For historical reasons, breast screening in ... Co-ordinating Group Cancer Services Co-ordinating Group 2000 22 Dewar JA, Twelves CJ, Thomson CS for the Scottish Breast Cancer Focus Group and the Scottish Cancer Therapy Network Breast Cancer in...
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
... of 575 nm (A) Fluorescence intensity analysis of intracellular adriamycin in gastric cancer cells (B) Adriamycin releasing index of gastric cancer cells The releasing index = (accumulation value ... retention in SGC7901/PrPC cells were further determined by flow cytometric analysis The adriamycin-releasing index in gastric cancer SGC7901 cells was calculated as follows: releasing index = (accumulation ... PI3K/Akt signaling pathway may lead to inhibition of the MDR induced by PrPC in gastric cancer cells The mechanism underlying PI3K/Akt-mediated PrPC-induced MDR in gastric cancer was further investigated...
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European guidelines for quality assurance in breast cancer screening and diagnosis pot
... screening should be established in line with the routine screening interval, taking into consideration that most programmes not succeed in keeping the routine screening interval for each individual ... screening should be established in line with the routine screening interval, taking into consideration that most programmes not succeed in keeping the routine screening interval for each individual ... continuing advances in technical and professional knowledge Certain principles remain just as important in diagnosis as they are in screening Training, multidisciplinary teamwork, monitoring...
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Ruta 6 selectively induces cell death in brain cancer cells but proliferation in normal peripheral blood lymphocytes: A novel treatment for human brain cancer doc
... reduced in Ruta 6treated cancer cells but not in normal B-lymphoid cells (Fig 4) FACS analysis data of Ruta 6-treated cells showed the accumulation of more subdiploid cells in MGR1 glioma cancer cells ... can induce regression of human glioma brain cancers in vivo This might be achieved by the induction of telomere loss in cancer cells as shown in our in vitro experiments with glioma-derived brain ... H2O2 only and in combination: A, human MGR1 brain cancer cells showing higher percentages of abnormal metaphases in H2O2- and Ruta 6-treated cells; B, normal human B-lymphoid cells showing more normal...
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Báo cáo khoa học: Vitamin D stimulates apoptosis in gastric cancer cells in synergy with trichostatin A ⁄sodium butyrate-induced and 5-aza-2¢-deoxycytidine-induced PTEN upregulation ppt
... declined in gastric cancer [35], we speculated that PTEN might participate in the VD3-induced apoptosis in gastric cancer cells To investigate this, undifferentiated HGC-27 adenocarcinoma cells ... melanoma cells, including those in myeloid leukemia, and breast, prostate, colon, skin and brain cancer [4– 8] Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was originally discovered ... transfected cell lines HGC-27 cells were transfected with pSliencer 4.1–CMV neo-siPTEN or pSliencer 4.1–CMV neo vector, using the Lipofectamine 2000 (Invitrogen) protocol Cells were maintained in IMDM...
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Báo cáo khoa học: Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells pot
... chemopreventive agents, including resveratrol [26,27], brassinin [28,29], and deguelin [30,31] Resveratrol is present in grapes, red wine and peanuts, brassinin is from Chinese cabbage, and deguelin is from ... apoptosis in SW480 human colon cancer cells To investigate the effects of IxoA on apoptosis, we 5720 used acridine orange ⁄ ethidium bromide staining and DAPI staining and found a marked increase in ... Conjugated linoleic acid inhibits peritoneal metastasis in human gastrointestinal cancer cells Int J Cancer 118, 571–576 Ogata S, Uehara H, Chen A & Itzkowitz SH (1992) Mucin gene expression in colonic...
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Targeting New Pathways and Cell Death in Breast Cancer Edited by Rebecca L. Aft docx
... human breast cancer cells to estrogen-induced apoptosis Breast Cancer Res 10(6): R104 Lewis, J S., K Meeke, et al (2005) Intrinsic mechanism of estradiol-induced apoptosis in breast cancer cells ... growth of breast cancer cells, to trigger the apoptotic signaling in breast cancers, thus switching the estrogenic hormones from potential mitogens to apoptosis inducers in breast cancers (Shi, ... HERs are commonly activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible...
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