antioxidative activity of chitosan chitooligosaccharides and their derivatives
Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... cDNA, and lanes 1, 3, and show the same using genomic DNA of C roseus Primer pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and ... #PFLF-1 and #PFLR-1 (lanes and 8) (B) Schematic organization of the CrPrx gene The asterisk indicates the position of the codon encoding the first amino acid of the mature protein, and the regions of ... membranes of the central vacuole, and the wall thickening of xylem cells (fi) common with other plant peroxidases [18,21,22] The inclusion of Ser96 and Asp99 in a salt bridge motif at the beginning of...
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Báo cáo khoa học: Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase pdf
... biological activity of the two compounds and usually only JA content is measured If values of both JA and MeJA are published, the ratios depend on the plant species and the tissue analysed and vary ... cycles of 30 s at 94 °C, at 40 °C and at 72 °C, followed by 30 cycles of 30 s at 94 °C, at 45 °C and at 72 °C Gel electrophoresis, DNA elution and modification was carried out according to standard ... speculated that the physical role of MeJA is to mobilize JA [19] The proof of this hypothesis might come from a more detailed understanding of how plants form MeJA from JA, and vice versa A recent study...
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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx
... to the understanding of the underlying mechanism of calcium metabolism in the process of shell and pearl formation, but also offer the opportunity to promote the yield and quality of pearl In this ... second and third EF-hand domains in oyster CaM and CaLP, while it reflected Ca2+-binding to the first and second EF-hand domains in the case of VanScyoc et al CaLP and CaM chromatography of extracts ... major component of oyster shell as well as a key intracellular second messenger The shells of oyster consist of 90% CaCO3, products of calcium metabolism, and a few percent of matrix of biological...
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Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx
... isolated two positive clones of 1324 and 1319 bp, designated DpAR1 and DpAR2, respectively The nucleotide sequences of DpAR1 and DpAR2 have been submitted to the EMBL database and are available under ... postulated to be alcohol dehydrogenases Nevertheless, the high degree of homology of their amino-acid sequences and those of the alfalfa and D purpurea ARs suggests that all these proteins belong to ... 2847 of the steroids assayed It is worth noting that this is the first report for such steroid activity from a plant AKR enzyme Expression of DpAR genes The size of the DpAR transcripts and their...
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Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
... kit TnT (Promega, Leiden, the Netherlands) under the control of T7 promoter using pcDNA3.HIS, and the quality of synthesis was verified by SDS/PAGE and exposure of the dried gel GSt or GStp76RBE ... representation of (A) the p76RBE protein and (B) the human genomic structure of p76RBE gene (A) Schematic representation of the 686 amino acid p76RBE protein with delimitation of both HR-1 and PDZ domains ... domains Regions of homology with proteins of other species are underlined and the percentage of amino acid identity is indicated (B) Schematic representation of the human genomic structure of p76RBE...
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Cloning and characterization of a novel kelch like gene in zebrafish
... life, and for being my unfailing source of strength and help and hope throughout these years and this thesis i Table of Contents TABLE OF CONTENTS Acknowledgements i Table of Contents ii List of ... µl of 70% ethanol and air-dried The DNA pellet was dissolved in µl of loading dye (50 ml contains ml of 25 mM EDTA, pH8.0; 10 ml of deionised formamide; 50 mg Dextran blue and 39 ml of H2O) and ... for minutes and cooled in ice immediately µl each of dGTP, dTTP and dCTP with 7.5 µl of Random Primers Buffer Mixture and 2.5 µl of 32 P-dATP was added to make up to a total volume of 25 µl after...
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Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx
... studies, high affinities and activities of CA had been found in the plume and in the trophosome CA from the branchial plume tissue had an affinity of 13.9 mmolÆL)1 and an activity of 253.7 lmol CO2Æmin)1Æg)1 ... branchial plume cDNA with an open reading frame of 726 nucleotides and 5¢- and 3¢ UTR sequences of 171 and 442 nucleotides, respectively Positions of the primers on the complete cDNA are given ... previously sequenced RpCAtr (BPNJ ¼ 100 and BPMP ¼ 99) Fungia scutaria (FCA-a and FCA-b) and Caenorhabditis elegans (CA1 and CA2) sequences fall outside of clade I and are more closely related to each...
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Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf
... analysis of metabolites Reactions were initiated by the addition of 100 ll of a solution of enzyme (200 lgÆmL)1) to 0.9 mL of a solution of 50 lg of substrate in 10% (v/v) dimethyl sulfoxide and incubated ... measurement of activity is described above To examine the enzymatic incorporation of 18O2 and 18O-labeled water into GG, reactions were initiated by the addition of 20 lL of a Enzymic reaction and analysis ... 0.1 mL of EC or CY to 1.0 mL of VM containing 10 lg of GOU For HP and M, reactions were initiated by addition of 0.1 g of HP or M to 1.0 mL of VM containing 10 lg of GOU After incubation for 12...
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Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx
... ACF7, neural isoform 2, is a member of the dystonin subfamily and the beta-spectrin superfamily Fig Preparation of an anti-ISP36 serum (A) Expression of human His6-ISP36 in E coli and its purification ... NaCl Animals Animal care, housing and killing were in accordance with the guidelines of the Ministry of Education, Science, Sports and Culture of Japan for the use of laboratory animals Cell fractionation ... Tween-20 for each, and detected using H2O2 and 3,3¢-diaminobenzidine This work was supported by a grant-in-aid from the Ministry of Education, Science, Sports and Culture of Japan and grants from...
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báo cáo hóa học: " The design and testing of a novel mechanomyogram-driven switch controlled by small eyebrow movements" docx
... sway their chair slightly, move their head and talk without moving their eyebrows or the strap The number of true positives (TP), true negatives (TN), Alves and Chau Journal of NeuroEngineering and ... Alves and Chau Journal of NeuroEngineering and Rehabilitation 2010, 7:22 http://www.jneuroengrehab.com/content/7/1/22 Page of 10 Figure Signal denoising The microphone signal and RMS values of the ... initial burst of activity facilitates the detection of contraction onset, the eventual decay in activity encumbers activity- detection during sustained contractions This limits the potential of a secondary...
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báo cáo hóa học: "Initial development and testing of a novel foam-based pressure sensor for wearable sensing" doc
... courtesy of the University of Bath, England, showed that the stress-strain profile of the unadulterated PU foam sample and that of the PPy-coated PU foams sample were similar showing regions of elastic ... durable and washable over an indefinite period of time In a garment-integrated context, washability of components is important to the preservation of normal user patterns of care and maintenance of ... variety of body shapes and sizes would be hard to predict Similar issues would arise with sizing, fit, and sensor locations on the foot Because of the increased number of sensors and precision of...
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Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx
... immunized with c19orf10-His fusion protein and the reactivity and specificity of the purified IgY were confirmed using GST-c19orf10 and a panel of unrelated His and GST fusion proteins The antibody ... clear understanding of the function of this protein The previous name, IL-25, was based on an apparent growth-factor activity [6] The claim of this activity was subsequently retracted and this has ... Expression of c19orf10 in OA synovium (a) Intense staining of the synovial lining layer and perivascular regions of RA (OCT section) tissue.(b) Intense staining of the synovial lining layer and perivascular...
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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx
... basis of our data this might be a consequence of the rapid conversion of CGA to DCQs as by means of an unknown downstream enzymatic step Indeed the involvement of the HQT gene in the profile of ... distances on LG5 and LG9 have been improved with the integration of the HQT and HCT genes The former increased the number of bridge markers on LG5, and reduced some large gaps (of 10 cM and cM) affecting ... N-hydroxycinnamoyl/benzoyltransferase group [51] and are closely related to their tobacco and tomato orthologues The hydroxycinnamoyltransferase activity of the enzyme and its involvement in PP biosynthesis...
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Báo cáo khoa học: "Identification and characterisation of a novel anti-viral peptide against avian influenza virus H9N" pps
... and treat infections Traditionally, the generation of new drugs involves screening hundreds of thousands of components against desired targets via in vitro screening and appropriate in vivo activity ... virus efficiently and the higher the concentration of the recombinant phages, the higher the signal irrespective of the concentration of the virus Antiviral activity of peptides and fusion phages ... their respective parental vectors, no β-gal activity was detected either The co-transformed BD-P1 and AD-HAt as well as BD-P1 and AD-NA showed comparatively high level of β-gal activity (25 and...
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Báo cáo y học: "Quantifying the major mechanisms of recent gene duplications in the human and mouse genomes: a novel strategy to estimate gene duplication rates" docx
... Figure contribution of unequal crossover and retroposition Relative Relative contribution of unequal crossover and retroposition 'TAG/Retro' is the ratio of the cumulative number of tandemly arrayed ... results of this study will further our understanding of the mechanisms of gene duplication in mammals Pan and Zhang R158.3 comment of the human genome [32], and usually not contain genes [33] Third, ... linear functions of which are Hn and Mn (red) The FAM2 gene set was plotted in bold lines, the linear functions of which are Hp1 and Mp1 (red) for the part with Ks ≤ 0.25, and Hp2 and Mp2 (green)...
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TARGETING ACUTE PHOSPHATASE PTEN INHIBITION AND INVESTIGATION OF A NOVEL COMBINATION TREATMENT WITH SCHWANN CELL TRANSPLANTATION TO PROMOTE SPINAL CORD INJURY REPAIR IN RATS
... activity of the mammalian target of rapamycin (mTOR) is a major inhibitor of the progression of apoptosis, and stability of mitochondrial function may result from PINK-1-induced upregulation of Akt activity ... highlights some of the most commonly used chemical inhibitors, their targets, and functions for assessing the roles of particular steps of these pathways and for experimental assessment of their benefits ... neurons and their targets in the PNS The role of SCs in repair of damaged axons Along with their role in myelination, SCs produce numerous trophic factors and regulators of cellular activity...
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Development and evaluation of a novel nanoparticulate delivery system of arsenic sulfides
... suspension with particle size of 100 and 150 nm and induction of apoptosis Ye HQ et al., College of Life Science and Technology, Huazhong University of Science and Technology, PRC Myeloid leukemia ... JZ and Ho PC, 2006] Inhibition of growth and promotion of apoptosis Observations of the clinical utility of realgar in the treatment of APL have triggered investigations into the mechanisms of ... among arsenic trioxide and realgar and orpiment in the activity and the mechanisms of action and basis of toxicity could be due to the difference in the valency states of these compounds The...
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Identifcation and stablization of a novel 3d hepatocyte monolayer for hepatocyte based applications
... Chromatograms of Arginine (a), hydrolysis product of soluble GRGDS peptide (b) and soluble galactose ligand (c) as standards, and hydrolysis product of the PET-Hybrid conjugated with GRGDS peptide and ... metabolic profiles and toxicity of hundreds of thousands of drug-like chemical compounds in order to screen out the promising candidates which can then enter the following animal testing and pre-clinical ... monolayer’, therefore combines the advantages of both gold standards of 2D and 3D hepatocyte in vitro culture models and meanwhile eliminate some of their instinct problems Since the 3D hepatocyte...
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Identification and characterization of a novel heart reactive autoantibody in systemic lupus erythematosus possible serological marker for early myocardial dysfunction
... in Iceland and Japan and highest in USA and France The overall prevalence was the lowest in Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden of SLE ... humor, kindness, and help Thanks to all staff of Deparment of Rheumatology and Immunology, Singapore General Hospital for their help and friendship ii Thanks to all staff of Department of Clinical ... THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2007 ACKNOWLEDGEMENT First of all, I would like to express...
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