antigens peptides and recombinant vaccinia vectors rvv
Báo cáo hóa học: " Molecular and macromolecular alterations of recombinant adenoviral vectors do not resolve changes in hepatic drug metabolism during infection" potx
... and μl of QuantumRNA™ 18S internal standard (Ambion, Austin, TX) at a primer:competimer ratio of 4:6 for CYP 3A2 and 2C11 and 3:7 for PXR and RXR reactions Gene-specific reaction conditions and ... distribution patterns and phosphorylation status of PXR and RXRα in vitro and in vivo will further support these hypotheses and are currently underway in our laboratory Materials and methods Figure ... determine how recombinant adenovirus alters CYP expression and function Discussion None of the modifications commonly made to reduce the immunogenicity and toxicity associated with adenoviral vectors...
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Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
... USA) Internalization and detection of recombinant proteins Recombinant GST±Tat protein and GST±Tat±GFP were prepared as already described [21] For direct detection of the GFP recombinant protein ... of peptides We also thank I Robbins for proofreading of the manuscript This work was supported by grants from the Association pour la Recherche sur le Cancer to B L and E V and from MURST and ... Probes Europe BV, Leiden, the Netherlands) in 50 mM Tris/HCl buffer pH 7.2 for h in the dark Labeled peptides were puri®ed by semipreparative HPLC, freeze-dried, and resuspended in NaCl/Pi at mgámL)1...
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Báo cáo khoa học: Evolutionary origin and divergence of PQRFamide peptides and LPXRFamide peptides in the RFamide peptide family pdf
... RFamide peptides T Osugi et al annelids, molluscs, and arthropods Invertebrate RFamide peptides produced within the nervous system can act as neurotransmitters and neuromodulators These neuropeptides ... years ago, and they therefore serve as a key animal in understanding the evolutionary history of PQRFamide and LPXRFamide peptides Here, we show that the lamprey brain possesses PQRFamide peptides ... al Novel lamprey RFamide peptides Fig Unrooted phylogenetic tree of the precursors of the identified lamprey PQRFamide peptides, and the identified and putative RFamide peptides in other vertebrates...
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Báo cáo Y học: Modulation of the oligomeric structures of HIV-1 retroviral enzymes by synthetic peptides and small molecules pptx
... from the a1 and a5 helices of both subunits Synthetic peptides corresponding to the respective sequences (93–107 and 167– 187) were found to strongly inhibit the 3¢-processing and strand-transfer ... (Eur J Biochem 269) 5105 Table Peptide and small molecule inhibitors of HIV-1 PR dimerization IC50 (lM) Peptides Derived from the Nand C- Termini of HIV-1 PR and MA Ac-Thr-Leu-Asn-Phe-COOH N-Pro-Gln-Ile-Thr-Leu-Trp-OH ... by the four termini in the dimer (residues 1–5 and 95–99) Additional interactions include a complex salt bridge between D29 and R87 of one subunit and R8 of the other subunit Thermodynamic analyses...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
... serum-derived and recombinant HCII Natural and recombinant HCII were characterized further by peptide mapping After reduction and carboxamidomethylation, the proteins were digested with trypsin and the ... Biochem 269) ¨ Table Amino-acid sequence and tryptic peptides of human HCII The peptides indicated below were detected by MALDI-TOF- and/ or ESI-MS (see Figs and 2, respectively) in tryptic digests ... tryptic peptides recorded by ESI-MS of (A) serum-derived HCII and (B) the recombinant protein from CHO cells The detected molecular ions of these peptides (43-ENTVTNDWIPEGEEDDDYLDLEK-65 and 66-IFSEDDDYIDIVDSLSVSPTDSDVSAGNILQLFHGK-101)...
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Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
... : 1, : and : of Tat2 and plasmid DNA However, before mixing, Tat2 and the DNA were prepared separately in 25 lL of sterile water The mixture was incubated for h for complex formation and subjected ... cell-penetrating peptide and recombinant proteins fused to Tat Eur J Biochem 269, 494– 501 13 Patel LN, Zaro JL & Shen W-C (2007) Cell penetrating peptides: intracellular pathways and pharmaceutical ... whereas other peptides (Tat2, pVEC and transportan) FEBS Journal 275 (2008) 2403–2414 ª 2008 Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food...
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Báo cáo khóa học: Comparison of native and recombinant chlorite dismutase from Ideonella dechloratans pot
... host, but not in the recombinant version of the enzyme Comparison of mass spectra for tryptic peptides obtained from the native and recombinant enzymes suggest HK(52– 53) and YHVR(220–223) as ... and T(242) (bold) were not covered To compare the native and recombinant enzymes, peptides were generated by in-gel trypsin digestion and subject to mass analysis using as above For the native ... of species giving rise to high- and low-spin signals were estimated as described in [27] and [28], respectively Results Electrophoresis of proteins and tryptic peptides We have previously reported...
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Báo cáo khoa học: Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 pptx
... (Millipore) and ể FEBS 2003 Kinetics of native and recombinant GAPDHs (Eur J Biochem 270) 131 eluted in 50% acetonitrile/0.1% triuoroacetic acid and 50% water/0.1% triuoroacetic acid Recombinant and ... for recombinant and native GAPDH with a multit using a common value of Km and different values of kcat The estimated parameters had a value of 25 lM for the Km, and the kcat for recombinant and ... 0.85 mM Recombinant (j) and native (h) GAPDH (1.5 ã 10)9 M and ã 10)9 M, respectively) were placed in the assay cuvette and the appearance of product was monitored Mean rate constants and standard...
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amino acids, peptides and proteins in organic chemistry
... Glycopeptides and Glycopeptide Vaccines 325 Synthesis of Tn, T, Sialyl-Tn, and Sialyl-T Glycosylated Amino Acid Building Blocks 325 Synthesis of Tn, T, Sialyl-Tn, and Sialyl-T Glycopeptides and ... BACE-1 Inhibitors 224 Paclitaxel and its Derivatives 228 References 234 Peptide Drugs 247 Chiara Falciani, Alessandro Pini, and Luisa Bracci Lights and Shades of Peptide and Protein Drugs Peptide Drugs ... Advances in b-Strand Mimetics 129 Wendy A Loughlin and David P Fairlie Introduction 129 b-Strands 129 b-Sheets 130 Differences in Strand/Sheet/Turn/Helix Recognition 130 Towards b-Strand Mimetics...
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hplc of peptides and proteins, methods and protocols
... peptide and protein purification continue to expand at an extremely rapid rate Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides ... the retention times of two retained solutes, and 2, are given by tr,1 and tr,2 The corresponding peakwidths for solutes and are denoted σ,1 and σ,2, and together with the retention times are they ... stationary and mobile phases, respectively, and Vs and Vm are the corresponding volumes of the stationary and mobile phases Since the ratio [X]s / [X]m is the equilibrium distribution coefficient K and...
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Báo cáo toán học: " Improved labelling of DTPA- and DOTA-conjugated peptides and antibodies with In-111 in HEPES and MES buffer" ppt
... Improved labelling of DTPA- and DOTA-conjugated peptides and antibodies with 111In in HEPES and MES buffer Maarten Brom*1, Lieke Joosten1, Wim JG Oyen1, Martin Gotthardt1 and Otto C Boerman1 Department ... DTPA-octreotide and DOTA-anti-CAIX antibody were five times higher in MES and HEPES buffer Radiolabelling of DTPA-exendin-3, DOTA-exendin-3 and DTPA-anti-CAIX antibody in MES and HEPES buffer ... than that in NaAc and NH4Ac Labelling of DTPA-exendin3 decreased with 66% and 73% for NaAc and NH4Ac, respectively, at day 11 after the production date of 111InCl3, while for MES and HEPES, the...
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báo cáo hóa học:" Improved labelling of DTPA- and DOTA-conjugated peptides and antibodies with In-111 in HEPES and MES buffer" doc
... Improved labelling of DTPA- and DOTA-conjugated peptides and antibodies with 111In in HEPES and MES buffer Maarten Brom*1, Lieke Joosten1, Wim JG Oyen1, Martin Gotthardt1 and Otto C Boerman1 Department ... DTPA-octreotide and DOTA-anti-CAIX antibody were five times higher in MES and HEPES buffer Radiolabelling of DTPA-exendin-3, DOTA-exendin-3 and DTPA-anti-CAIX antibody in MES and HEPES buffer ... than that in NaAc and NH4Ac Labelling of DTPA-exendin3 decreased with 66% and 73% for NaAc and NH4Ac, respectively, at day 11 after the production date of 111InCl3, while for MES and HEPES, the...
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Báo cáo hóa học: " A Genetic Programming Method for the Identification of Signal Peptides and Prediction of Their Cleavage Sites David Lennartsson" pot
... training set consisting of 176 background proteins and 291 signal peptides and one validation set consisting of 75 background proteins and 125 signal peptides For every position in the peptide sequence, ... task by which they are graded, and the more fit individuals survive and proliferate The solution candidates, or the individuals, have two appearances, the genotype and the phenotype The genotype ... 1986 [2] H Nielsen, J Engelbrecht, S Brunak, and G von Heijne, “A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites,” Int...
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Enzymes and Recombinant enzyme technique. docx
... fungi and plants Baby food Tripsin Recombinant enzyme technique (ReE) • Difinition: ReE are proteins which are produced by recombinant DNA technique • History of recombinant DNA tachnique: The recombinant ... of recombinant DNA: identification and cloning the insulin DNA and plasmid DNA are mixed together with ligase enzyme the sticky end bases form hydrogen bonds the ligase joins the DNA backbone and ... is a non-protein substance which may be organic, and called a coenzyme • Final enzyme: cofactor + apoenzyme Mechanism of enzyme action • Lock and key model: • Induced model: Factors affecting...
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Báo cáo y học: " Control of disseminated intravascular coagulation in Klippel-Trenaunay-Weber syndrome using enoxaparin and recombinant activated factor VIIa: a case report" pptx
... compiled the figures LAV and MLS edited the manuscript All authors analyzed and interpreted the patient data regarding the hematological disease and its management All authors read and approved the final ... exploratory laparotomy showed a subcapsular tear and hematoma Enoxaparin was suspended while numerous transfusions, aminocaproic acid and, for the first time, recombinant activated factor VII (rFVIIa, ... syndrome using enoxaparin and recombinant activated factor VIIa: a case report Journal of Medical Case Reports 2010 4:92 Author details Division of Pediatric Hematology and Oncology, Department...
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Báo cáo y học: " Management of subtrochanteric femur fractures with internal fixation and recombinant human bone morphogenetic protein-7 in a patient with osteopetrosis: a case report" pot
... mesenchymal precursors from muscle and surrounding tissues into the fracture site, inducing osteoblast and chondrocyte differentiation, and inducing angiogenesis and eventual bone formation [11] ... difficult and timeconsuming An eight-hole DCS plate with a 65 mm lag screw was used and this was secured to the femur with seven 4.5 mm cortical screws The wound was closed over a drain and our ... healing progression of the right femoral fracture and the stress fracture now visible in the left femur and better demonstrated in (b) Golden and Rodriguez Journal of Medical Case Reports 2010,...
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Báo cáo khoa học: " Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences" docx
... dividing and non-dividing cells in vitro and in vivo and of infecting cells originating from multiple species and tissue types No human disease has been found to be associated with AAV infection and ... constructs and contributed to the conduct of the experiments, analysis of the data, and preparation of the manuscript SM and RD contributed to the conduct of the experiments and data collection and ... downstream promoter, p19 Rep52 and Rep40 have been implicated in AAV single-stranded DNA formation and gene regulation while the two larger Rep proteins (Rep78 and Rep68) appear to convey the...
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Báo cáo y học: "The use of citrullinated peptides and proteins for the diagnosis of rheumatoid arthritis" pps
... Innala and colleagues [41] 210/102 98.0 80.4 69.0 ND ND Mutlu and colleagues [37] 93/83 98.8 57.0 to 60.2l,f 60.2g 29 ND 48.4 Soos and colleagues [52] 119/118 95 74.8 ND 69.7 ND 33.6 Vander Cruyssen ... (%) 124/158 95.2 73.1 92.3 70.5 76.7 88.2 Coenen and colleagues [44] 133/165 Correia and colleagues [39] 86/90 Dos Anjos and colleagues [40] Liu and colleagues [33] CCP2 CCP3 a a 89.7 to 91.4 87.1 ... 77.4 Lutteri and colleagues [24] 120/170 77.9 79.5 Sghiri and colleagues [29] 170/309 91.1 86.3 66.0 84.7 58.6 79.9 Soos and colleagues [52] 119/118 97.6 74.4 90.0 78.8 80.2 74.0 Ursum and colleagues...
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Báo cáo y học: " Diagnostic value of anti-cyclic citrullinated peptides and association with HLA-DRB1 shared epitope alleles in African rheumatoid arthritis patients" ppsx
... Zurich, Switzerland), were used to detect IgM and IgA RF The assays and calculations were performed according to the manufacturer’s instruction Each kit included its own RF standard and the results ... Switzerland Authors’ contributions MSN recruited the patients and collected the samples MSN and CG designed the study SB and J-MT performed the analysis AF performed the statistical analysis MSN and ... Switzerland), and those detecting the IgG anti-CCP3 (Quanta Lite CCP3: third generation of CCP antigen), were purchased from Inova Diagnostics (Ruwag, Zurich, Switzerland) The assays and calculations...
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Báo cáo y học: "HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells" pot
... HIV-1 X4 and R5 laboratory strains represented by HIV-1 IIIb and HIV-1 ada respectively Total DNA, collected and purified at days and postinfection, was analyzed by PCR, and both HIV-1IIIb and HIV-1ada ... blockade determined a clear recovery of vWF protein and mRNA expression at day as well as for Flt-1 and KDR (Figures and 10 and data not shown) in HIV-1 and gp120 treated samples Discussion Human MSCs ... p24 pAb (panel A) and p5p (panel B) Total RNA was extracted and purified at day and vWF, Flt-1 and KDR mRNAs were analysed The results were expressed by the ratio between samples and the control...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng 9 tr 25