antibodies flow cytometry and lymphocyte subset quantification
báo cáo hóa học:" Static platelet adhesion, flow cytometry and serum TXB2 levels for monitoring platelet inhibiting treatment with ASA and clopidogrel in coronary artery disease: a randomised cross-over study" potx
... and flow cytomCorrelation between static platelet adhesion and flow cytometry Correlation between ADP-induced platelet adhesion (Factor 1) and ADP-induced platelet activation as measured by flow ... Figure The influence of ASA and clopidogrel on platelet activity measured by flow cytometry The influence of ASA and clopidogrel on platelet activity measured by flow cytometry The effects of platelet ... completing the study (n=29) Figure Flow chart showing the inclusion of patients and controls Flow chart showing the inclusion of patients and controls Patients and controls were included consecutively...
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Báo cáo khoa học: " Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules" ppsx
... T lymphocytes?) Pan B Pan B Pan B Pan B and subpopulation of CD4 and CD8 T lymphocytes Pan B and subpopulation of CD4 and CD8 T lymphocytes B subpopulation B subpopulation Pan lymphocyte Pan lymphocyte ... basophils and a subset of CD4 T lymphocytes The subset of B lymphocytes detected with RACT14A and RACT21A was in low frequency in peripheral blood and in high frequency in spleen and appendix The subset ... CD8 CD4 and CD8 subpopulations Basophils and subpopulation of CD4+ T lymphocytes Granulocytes, basophils, and monocytes Subpopulations of B and T lymphocytes Subpopulations of B and T lymphocytes...
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Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"
... Cycle and Cell Death Analysis The effects on the cell viability and the cell cycle distribution were determined by DNA flow cytometry Flow cytometric analyses were performed using a PAS II flow ... diploid lymphocytes was 0.8 - 1.0 Cell viability and apoptotic cells were assessed by flow cytometry with propidium iodide (PI)-method For detection of apoptotic cells and viability a FACS Calibur flow ... DNA-index and cell cycle For histogram analysis we used the Multicycle program (Phoenix Flow Systems, San Diego, CA) Human lymphocyte nuclei from healthy donors were used as internal standard for...
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Báo cáo y học: "Flow cytometry analysis of glucocorticoid receptor expression and binding in steroid-sensitive and steroid-resistant patients with systemic lupus erythematosus" pps
... GR-positive T lymphocytes and monocytes in SLE patients and controls (a) Percentage of T lymphocytes and monocytes presentscontrols ing GR The percentage of positive T lymphocytes and monocytes ... in T lymphocytes (CD3/GR) and monocytes (CD14/ GR) of SR, SS patients and controls were measured by FCM before treatment The results showed that the percentage of GR-positive T lymphocytes and ... positive lymphocytes and monocytes presenting GR in SLE patients and normal controls No difference was found between the results of patients and controls Similar results were obtained by Wasilewska and...
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Báo cáo y học: "The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives" pot
... [11-13] Principle of basophil activation test by flow cytometry As flow cytometry is a valuable tool for the analysis of many different cell types and can be used to identify specific populations ... Fluo Figure Principle1of the basophil activation test by flow cytometry (triple staining) Principle of the basophil activation test by flow cytometry (triple staining) Basophils are identified on ... FITC-CRTH2 PE-CD203c Figure Identification of CRTH2 expressing cells by flow cytometry Identification of CRTH2 expressing cells by flow cytometry Left histogram : ungated leukocytes biparametric representation...
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Báo cáo y học: "Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts" pps
... cell cycle Cancer Res 2005, 65:7733-7742 Bonetta L: Flow cytometry smaller and better Nat Methods 2005, 2:785-795 Tree Star Inc: FlowJo Ashland, OR: Tree Star Inc; 2006 BD Bioscience: CellQuestPro ... corresponds to cell size and SSC to cell granularity Several subpopulations can be distinguished: (I) healthy and well measured cells, (II) cell debris, and (III) cell conjugates and air bubbles (b) ... inference Flow cytometry provides individual measurements for each cell of a population, and so we should like to use statistical procedures to model the behavior of the whole population and to...
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Báo cáo y học: "Long Term Persistence of IgE Anti-Influenza Virus Antibodies in Pediatric and Adult Serum Post Vaccination with Influenza Virus Vaccine"
... anti-influenza antibodies over time were not determined Given the findings of this study, the presence and persistence of IgG and IgE anti-influenza antibodies in the serum from those with normal and ele- ... conditions (50-64 y/o), and for children months and older [21, 25] In some countries, vaccination is recommended for health care workers, and caregivers in nursing homes and assisted living facilities ... vaccination, and then was two-fold lower moths post vaccination [31]; influenza specific IgG1 antibodies were the dominant antibody detected, as well as lower levels of IgM and IgA [31]; IgE antibodies...
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Undaria pinnatifida Habitat Loss in Relation to Sea Urchin Grazing and Water Flow Conditions, and Their Restoration Effort in Ogatsu Bay, Japan
... seaweed and aquaculture Midori Press, Tokyo, pp 133 - 144 Turner, M G., Gardner, R H and ÓNeill R V (2004) Landscape ecology in theory and practice: Pattern and process, Nakagoshi, N and Hara, ... surveys of the water velocities at randomly selected ten U pinnatifida habitats and twenty unvegetated areas in Line and Line were conducted in September 2004 and August 2005 Each area for the ... from the bottom and thus, allowing the recovery of U pinnatifida and other non-encrusting macroalgae Both buoyed reefs were made of wood and their length was 1.0 m with 1.0 m width and 16 cm height...
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Tài liệu Báo cáo " DETERMINING FLOW ENERGY AND EROSION COEFFICIENT FOR CLASSIFICATION OF POTENTIAL EROSION IN BINH DINH PROVINCE " ppt
... reclassification was carried out for upstream protection land (UPL), production forest land (PFL), convertible land (CL), cultivated land and plain as shown in Table The results show that high erosion ... potential mainly distributes on the convertible land, the upstream protection land, and the production forest land However, we have only researched and categorized the erosion potential in Binh ... and has total area of 39.307 hectares (43.4% in the convertible land) This group has low centralization level of convertible land and low erosion potential level + Group of the convertible land...
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Clinical Flow Cytometry – Emerging Applications Edited by Ingrid Schmid ppt
... permeabilized and stained with mAbs directed against IL-1 and CCR5, IL-2 and CCR5, IFN and CCR5, IL-4 and CCR4, or IL-10 and CCR4 and were analyzed on a flow cytometer A, B, C, D, and E FACScan ... Anti-Tumoural and Immune Modulatory Activity of Natural Products by Flow Cytometry 91 Susana Fiorentino, Claudia Urueủa, Sandra Quijano, Sandra Paola Santander, John Fredy Hernandez and Claudia ... eosinophils, lymphocytes and macrophages, and induce the production of antibodies and cytokines These cells are often found in damaged tissue (Luscinskas & Gimbrone, 1996) Clinical Flow Cytometry...
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ECOLOGY OF COLD SEEP SEDIMENTS: INTERACTIONS OF FAUNA WITH FLOW, CHEMISTRY AND MICROBES potx
... core (69 and 75 species) than the more active base (29 and 37 species) (Dando et al 1991) A detailed comparison of dominant nematode families and genera at the Hatsushima seep, the East Flower ... widespread, occurring in all continental margin environments (tectonically active and passive) and even inland lakes and seas It is safe to say that probably only a small fraction of existing seafloor ... fractures and sand layers or via mud diapirs Cold seeps are commonly found along fractures at the crests of anticlines, on the faces of fault and slump scarps where bedding planes outcrop and along...
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flow cytometry protocols 2d ed - teresa s. hawley
... flow cytometry instruments, in Flow Cytometry and Sorting, 2nd edit (Melamed, M R., Lindmo, T., and Mendelsohn, M L., eds.), Wiley-Liss, New York, pp 27–44 47 Pinkel, D and Stovel, R (1985) Flow ... history of flow cytometry and sorting Methods Cell Biol 63(pt A), 3–17 15 Van Dilla, M A., Dean, P N., Laerum, O D., and Melamed, M R., eds (1985) Flow Cytometry: Instrumentation and Data Analysis ... Cytometry 10, 229–241 32 Gray, J W and Cram, L S (1990) Flow karyotyping and chromosome sorting, in Flow Cytometry and Sorting, 2nd edit (Melamed, M R., Lindmo, T., and Mendelsohn, M L., eds.), Wiley-Liss,...
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flow cytometry protocols
... has made flow cytometry very popular and wtdely used The applications of flow cytometry are diverse and include the mterrogatlon of membrane, cytoplasmic, and nuclear antigens Flow cytometry ... York Melamed, M., Lmdro, T., and Mendelsohn, M., eds (1990) Flow Cytometry and Cell Sortzng, 2nd Ed Wiley-Liss, New York Parks, D and Herzenberg, L (1989) Flow cytometry and fluorescence-activated ... Practical Flow Cytometry 3rd ed LISS, New York Owens, M A and Loken, M R (1995) Flow Cytometric Prwczples for Clwucal Laboratory Practice Wiley-Llss, New York Radbruch, A., ed (1992) Flow Cytometry and...
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báo cáo hóa học:" Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist" potx
... validating specifically flow cytometry PD assay have been proposed These guidance and white papers can serve as a template or guide for the validation of a flow cytometry PD assay Flow cytometry PD assays ... instrument platforms, reagent quality and the increase in clinical usage have driven flow cytometry to be highly reproducible and consistent For these reasons, flow cytometry has become an excellent ... criteria and rigor that would accompany a validated flow cytometry assay [16] Using the guidance for ligand binding assays [12] as a foundation in which to base the validation of a flow cytometry...
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Báo cáo hóa học: "Exhaustive expansion: A novel technique for analyzing complex data generated by higherorder polychromatic flow cytometry experiments" pot
... and AR performed the research JCS and WM contributed vital analytical tools JCS, LW, AR, BZ, and EBW analyzed and interpreted the data JCS and EBW wrote the manuscript All authors have read and ... http://www.translational-medicine.com/content/8/1/106 Page of 15 Table Representative input and output for the “Expander” program analytical software such as FlowJo (http://www.flowjo com) and FCS Express (http://www.denovosoftware com) ... therapies [11] Thus, the use of higherorder flow cytometry and comprehensive multiparameter data analysis could facilitate the identification and expansion of T CM and T CM precursor subpopulations (i.e...
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Báo cáo sinh học: " Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice" pot
... productivity and the flexibility and potential speed associated with yeast expression system may prove to be indispensable during pandemic influenza outbreak Materials and Methods Yeast strain and growth ... forward and H1N1HA reverse primers A total of twenty clones were randomly picked up from all the three categories (3, and 11 clones each from 250µg/ml, 500µg/ml and 750µg/ml plates o respectively) and ... proximal stalk and a membrane distal globular head domain The globular head stands independently from the central stalk and contains the majority of the neutralizing antibody epitopes and the HA...
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Báo cáo hóa học: " Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice" pot
... productivity and the flexibility and potential speed associated with yeast expression system may prove to be indispensable during pandemic influenza outbreak Materials and Methods Yeast strain and growth ... forward and H1N1HA reverse primers A total of twenty clones were randomly picked up from all the three categories (3, and 11 clones each from 250µg/ml, 500µg/ml and 750µg/ml plates o respectively) and ... proximal stalk and a membrane distal globular head domain The globular head stands independently from the central stalk and contains the majority of the neutralizing antibody epitopes and the HA...
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CLINICAL FLOW CYTOMETRY – EMERGING APPLICATIONS pot
... permeabilized and stained with mAbs directed against IL-1 and CCR5, IL-2 and CCR5, IFN and CCR5, IL-4 and CCR4, or IL-10 and CCR4 and were analyzed on a flow cytometer A, B, C, D, and E FACScan ... Anti-Tumoural and Immune Modulatory Activity of Natural Products by Flow Cytometry 91 Susana Fiorentino, Claudia Urueủa, Sandra Quijano, Sandra Paola Santander, John Fredy Hernandez and Claudia ... eosinophils, lymphocytes and macrophages, and induce the production of antibodies and cytokines These cells are often found in damaged tissue (Luscinskas & Gimbrone, 1996) Clinical Flow Cytometry...
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SEDIMENT TRANSPORT – FLOW PROCESSES AND MORPHOLOGY pdf
... Berndtsson, Raj Deva Singh and S.N.Panda Chapter Bed Forms and Flow Mechanisms Associated with Dunes Ram Balachandar and H Prashanth Reddy Chapter Stochastic Nature of Flow Turbulence and Sediment Particle ... (51) where Qs(o) and Qs(c) are observed and computed total sediment outflow, respectively RE(Qs) and RE(Qps) are relative errors in total sediment outflow and peak sediment flow rates, respectively ... (Re ≥ 2000) and accompanied by rapid changes in velocities and pressures, in space and time Eddies and swirls are present in turbulent flow 36 Sediment Transport – Flow Processes and Morphology...
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SEDIMENT TRANSPORT – FLOW PROCESSES AND MORPHOLOGY pps
... Berndtsson, Raj Deva Singh and S.N.Panda Chapter Bed Forms and Flow Mechanisms Associated with Dunes Ram Balachandar and H Prashanth Reddy Chapter Stochastic Nature of Flow Turbulence and Sediment Particle ... (51) where Qs(o) and Qs(c) are observed and computed total sediment outflow, respectively RE(Qs) and RE(Qps) are relative errors in total sediment outflow and peak sediment flow rates, respectively ... (Re ≥ 2000) and accompanied by rapid changes in velocities and pressures, in space and time Eddies and swirls are present in turbulent flow 36 Sediment Transport – Flow Processes and Morphology...
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