... described in the Materials and methods. Completion ofthe hydrolysis wasconfirmed by the disappearance ofthe (Met1)-ONC(M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The ... calculating the slope of the curves of Pyr-ONC (M23L) vs. time. From the rates of cyclizationshowninTable1,itcanbeinferredthattheformation ofthe N-terminal pyroglutamyl is not dependenton pH in the ... hydrogen bonded to the e-amino group ofthe Lys9 side-chain, which simultaneouslyinteracts with the main phosphate ofthe substrate [32]. The closer positioning ofthe N-terminus to the mainprotein...
... fold, while the S15 contactsabove the internal loop of helix 22 and to the tetraloop of helix 23a stabilize the tertiary fold of the upper three-helix junction. The tertiarystructure ofthe upper ... regions ofthe central domainRNA are not involved in directing assembly of the domain but rather are displayed on the sur-face of a preassembled RNP core. Hence, for-mation ofthe core ofthe ... and S18. The structure ofthe complexdemonstrates the central role of S15-induced reorganization of central domainRNA for the subsequent steps of ribosome assembly. The recent explosion of structural...
... loop in the primed side ofthe binding sitegroove. Although the prodomains of cathepsins F andW share the same degree of similarity to the prodo-main of cruzipain, the similarity ofthe mature ... type of N-terminal tag was previ-ously used for the expression ofthe propeptide of human cathepsin K, and it did not interfere with the inhibitory activity ofthe protein [24]. Although some of the ... RGVDLQPS-LIS at the C-terminus, which resulted from the fusion of the propeptide with a small stretch of coding sequencewithin the multiple cloning site ofthe pQE30 plasmidbefore the stop codon. The predicted...
... and the Wellcome Trust. The authors would like to thank the Biotechnology and Biological SciencesResearch Council, the Medical Research Council and the European Union for financial support for these ... FAD/NADPH domain(% 16-fold) (29). However, the domain mixture still recon-stituted only % 2% ofthe activity ofthe full length CPR. InthecaseofNR1,thekcatfor the full length enzyme is low(1.3 s)1) ... and the oxidized FMNdomain. The lack of a second order dependence of the observed rate for interdomain electron transfer as the Fig. 4. Absorbance vs. potential plots for the FMN domain of human NR1....
... (JASCO).Fig. 3. Alignment ofthe sequences ofthe second Kunitz-modules ofthehuman and fugu WFIKKN proteins (wfikkn_hu_2; wfikkn_fugu_2) with the second Kunitz-domains ofthehuman and the two fugu WFIKKNRP ... determinant ofthe hydrophobic SÂ2 bindingpocket of trypsins is the side-chain of Tyr151 [18]. The importance of this residue is underlined by the fact that in the case ofthe second Kunitz-domain of ... a product of Fluka (Buch,Switzerland).Cloning and expression ofthe second Kunitz-typeprotease inhibitor module ofhuman WFIKKN proteinOn the basis ofthe known sequence ofthe human WFIKKN...
... abuse their office andinfluence to the injury ofthe laws and peace ofthe country. The elimination ofthe Christian teachers of the Negro race, and the prevention ofthe immigration of workers ... as their peculiar needs demanded. In the agricultural sections the first duty ofthe teacher ofthe blacks was to show them how to get their living from the soil. This was the final test of their ... _Special Report of the U.S. Com. of Ed_., 1871, p. 391.] The settlers of North Carolina followed the same procedure to check the influence of Quakers, who spokethere in behalf ofthe man of color...
... Child-OIDP. The self-administered ver-sion ofthe Child-OIDP performed similarly to the inter-viewer-administered original version ofthe instrument.Despite the demonstrated appropriateness ofthe ... Child-OIDP and some ofthe asso-ciations between the Child-OIDP and proxy measureswere not significant. While the low value of alpha isaffected by the nature ofthe Child-OIDP index and the fact that ... self-administration ofthe index. Furtherstudies are needed on the comprehensive psychometricevaluation ofthe self-administered Child-OIDP.Declaration of competing interests The authors declare that they...
... possible difficulty in intra-articular fracture ofthe distal radius is the displacement tendency of the radial styloid process due to the tension ofthe brachioradialis tendon.Methods: Ten patients ... drafting ofthe manuscript.GK participated in the design ofthe study and coordination and helped todraft the manuscript. All authors read and approved the final manuscript.Competing interestsThere ... in the interval between the flexor carpi radialis tendon and the radial artery. The distal part ofthe pronator quadratus muscle wasreleased from the radius. Care was taken to release onlythe...
... production of biomass of woody origin exceeds the food needs ofthe stand-ardized stocks of hoofed ruminant game roughly 4 times in the studied area. In the interest of sup-port ofthe red ... forested part ofthe studied area is 54.According to the decision ofthe Department of Environment of Břeclav MÚ, Ref. No. MUBR 4789/2004 OŽP-Sf, the standardized stocks of game in the game preserve ... MJ. To assess the sufficient food amount from the aspect of quality, the need for energy was calculated on the basis ofthe metabolic Table 2. Calculation of conversion units of hoofed game on...
... tragedy, so the early resolution of any doubts as to the validity of the report was a matter of great public concern. Also thereport contained very severe criticism of certain senior officers of Air ... the end only expressions of opinion. They would not even be admissible in evidence in legal proceedings as to the cause of a disaster. In themselves they do not alter the legal rights ofthe ... certain that the findings ofthe Erebus Commission would be published by the Government. The effect on the reputation of persons found guilty ofthe misconduct described in theReport was likely...
... niGeria report 2012: improvinG the Lives of GirLs and Women in niGeriaã Only 4% of females complete secondary school in the Northern zones.ã Over half of all women in the North are married by the ... niGeria report 2012: improvinG the Lives of GirLs and Women in niGeria 5 2 the deveLopment ConteXtThis section provides the background and economic context for a discussion ofthe position of women ... only 20% of enterprises in the formal sector (World Bank, 2009: 92). 23% of these enterprises are in the retail sector. Women make up 37% ofthe total work force in the garment industry. They are...
... of their posts; and it is "armed with the tremendous weapons" which slay legislation. And if the voice ofthe Church was the voice of God, so the voice oftheCourt is the voice of ... from the doctrine ofthe separation of powers—that, while the function of making new law belongs to the legislative branch of the Government, that of expounding the standing law, of which the ... humiliation to the latter and an utter disparagement of State Rights. Even more important than the relation ofthe Supreme Court to the judicial systems of the States was the question of its relation...
... describes the work and transmits the final reportofthe Committee on Proposal Evaluation for Allocation of Supercomputing Time for the Study of Molecular Dynamics, Second Round. The committee ... system housed at the Pittsburgh Supercomputing Center (PSC), based on the advice of a previous National Research Council committee convened in the fall of 2010. The success ofthe program has ... of Sciences. All rights reserved.10% off print titlesCustom notication of new releases in your eld of interestSpecial offers and discountsNATIONAL ACADEMY OF SCIENCESNATIONAL ACADEMY OF...
... from the negligent performance by the AIFM of its professional duties i.e. the risks to be covered are the risks of losses arising from the professional activities ofthe AIFM for which the ... particular AIFM and the calculation ofthe value of assets under management by the AIFM on behalf of these AIFs. 2. It is the responsibility ofthe AIFM to establish whether it must obtain ... in respect ofthe arrangements for managing the liquidity of AIFs under their management (and the percentage of assets subject to special arrangements), the risk profile of AIFs and the systems...
... affiliates. For internal use of Judicial Councilof California Administrative Office ofthe Courts only. Judicial Councilof California Administrative Office ofthe Courts 31 December 2007—Page ... percent ofthe state’s court caseload. Three ofthe installations (Los Angeles, Orange, and San Diego) host their own instances of both the application and the database. The rest ofthe counties ... rights reserved. Gartner is a trademark of Gartner, Inc. or its affiliates. For internal use of Judicial Councilof California Administrative Office ofthe Courts only. Judicial Council of...