angle sum property of a triangle activity
Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc
... aa208 aa24 aaBamH34 aaStartStopXho1N-Pro Protease domainProtease domainCT - ex380 aa1IINP114 aa208 aaBamH134 aaStartStopXho1SS SSGSSSSN-Pro356 aaIIIMSTLFIISILLFLASFSYAMDISTIEYKYDKSSAWRTDEEVKEIYELWLAKHDKVYSGLVEYEKRFEIFKDNLKFIDEHNSENHTYKMGLTPYTDLTNEEFQAIYLGTRSDTIHRLKRTINISERYAYEAGDNLPEQIDWRKKGAVTPVKNQGKCGSCWAFSTVSTVESINQIRTGNLISLSEQQLVDCNKKNHGCKGGAFVYAYQYIIDNGGIDTEANYPYKAVQGPCRAAKKVVRIDGYKGVPHCNENALKKAVASQPSVVAIDASSKQFQHYKSGIFSGPCGTKLNHGVVIVGYWKDYWIVRNSWGRYWGEQGYIRMKRVGGCGLCGIARLPYYPTKAAGDENSKLETPELLQWSEEAFPLAIV A B66 ... themaximum activity using an azocasein assay at different tempera-tures, as described in Materials and methods. Each data point is anaverage of three independent experiments having similar values(Table ... 2011 The Authors Journal compilation ê 2011 FEBS cysteine proteases [Erv -A, -B and –C (isolated from thelatex of Ervatamia coronaria in our laboratory) and papainfrom Carica papaya (Merck,...
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Tài liệu Báo cáo khoa học: Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping ppt
... biological systems that utilisedomain swapping to regulate activity. For example,glyoxylase I of Pseudumonas putida is a metastabledomain-swapped dimer with two Zn2+cofactors thatalso exists as ... can be isolated by Cibacron Blue affin-ity chromatography. (A) Unusual behaviour of TorD on CibacronÔBlue affinity media. A sample of 0.5 mM metal affinity chromatogra-phy-purified TorD was applied ... forGTPase activity, although this variant demonstratedenhanced XTPase activity (Fig. 6A, B). To test for anincreased affinity for xanthine nucleotides by a differentmeans, the standard malachite...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
... mutationsMousumi Banerjee1, Hemalatha Balaram2and Padmanabhan Balaram11 Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India2 Molecular Biology and Genetics Unit, Jawaharlal ... Ravindra G & Balaram P (2005) Plasmodiumfalciparum triosephosphate isomerase: new insights intoan old enzyme. Pure Appl Chem 77, 281–289.20 Parthasarathy S, Ravindra G, Balaram H, Balaram ... inenzymes: a study of triosephosphate isomerase andcomparison with methyl glyoxalsynthase. Adv ProteinChem 66, 315–372.45 Gunasekaran K, Ramakrishnan C & Balaram P (1996)Disallowed Ramachandran...
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Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt
... abzymes; nitrosoalcanes;microperoxidase 8; S-oxidation.Catalytic antibodies with a metalloporphyrin cofactor, orÔhemoabzymesÕ, are not as efficient a category of catalysts astheir natural hemoprotein ... set of six monoclonalantibodies was thus obtained: the best peroxidase activity –that found with the complex of MP8 and one of thoseantibodies, 3A3 – was characterized by a kcat/Kmvalue of 2 ... S-oxidation of thioanisoleAs the above results showed that the best system for theS-oxidation of thioanisole associated H2O2as an oxidantwith MP8 as a catalyst in the presence of tBuOH as anorganic...
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Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt
... a positive or negative way. Muta-tional analysis has indicated residues that are important forselectivity and potency, and structural analyses of suchmutants have suggested that what appear ... mutational analysis has been carried out onImI, PnIA, PnIB and to a lesser extent on GID and MII.Analysis of ImI has revealed that Asp5-Pro6-Arg7 andTrp10 are important for biological activity ... 12 (Table 1) appears to contribute to a4 b2anda7subtype activity but not a3 b2 activity. A decrease in a4 b2and a7 subtype activity but not a3 b2 activity was observedin the [R1 2A] GID mutant [16].An...
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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... take place at the N-terminalregion of P40, as a bacterial signal peptide had beenpredicted [23–25]. Such signal peptides are normallycleaved off by a bacterial type I signal peptidase(SPase). ... of the gene product of MPN 142. Cleavage into P40 and P90 takes place after amino acid454. The N-terminus of the mature P40 starts with amino acid 26.The molecular mass of about 36 000 Da of ... pathogenic bac-terium [1,2], characterized by a small genome of 816kbp [3], the lack of a bacterial cell wall and a parasiticlifestyle [4].Some species of the genus mycoplasma, e.g. Myco-plasma genitalium,...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... SDS/PAGE analysis. After 24 h, the fraction of molecules behaving as a substrate for uPA decreasedapproximately twofold for PAI-1(V12 6A) , PAI-1(F10 0A) ,PAI-1(F12 8A) and PAI-1(W14 1A) with a concomitantincrease ... half-life of PAI-1 was finally calculated from an exponential decay plot of the data obtained. Generally, only one preparation of each PAI-1 variant was investigated, but the following wereinvestigated ... significantly faster latency transition rateand another component with a significantly slower latencytransition rate. The activity of the three variants PAI-1(N13 9A) , PAI-1(W14 1A) and PAI-1(T14 6A) ...
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Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt
... i.e. agonistically as wellas antagonistically, completely inactive. The lack of antagonistic activity may be explained by the fact thatthis lipid A does not intercalate into target cell membranesby ... in a membrane-bound form in which it isable to cause an intercalation of LPS into this membrane[41]. It can be assumed that membrane proteins such asCD14 may also cause a membrane intercalation ... Chicester.32. Muăhlradt, P.F. & Frisch, M. (1994) Purification and partial bio-chemical characterization of a Mycoplasma fermentans-derivedsubstance that activates macrophages to release nitric oxide,tumor...
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Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx
... 5Â-CCATCCTAATACGACTCACTATAGGGC-3Â) and a gene-specic sense primer (5Â-GCCGCTCGAGTTTGAGTTAGCCAGAAACTCC-3Â, XhoIsite underlined) or antisense primer (5Â-ATACGGGCCCGACAGTGGTGTGCCTGGGAAC-3Â, ... the results of Hasegawa et al. [13] who questioned the paraphyly of theorder rodentia using the same data as Graur. In contrast,the distance matrix algorithms again placed the guinea pigtogether ... 11b-hydroxylase activity of CYP11B1 was strongly reduced(Fig. 5), whereas that of the aldosterone synthase wasbasically unaffected, while the 18-hydroxlase and oxidase activity was also greatly diminished....
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Báo cáo khoa học: The oleic acid complexes of proteolytic fragments of a-lactalbumin display apoptotic activity pdf
... Chester, PA, USA). The percent-age of cell death and the standard deviation were calculatedfrom three acquisitions of each treatment. The data arereported as percentage of BAMLET activity. AcknowledgementsWe ... theprotein.Abbreviations a- LA, a- lactalbumin; BAMLET, bovine a- lactalbumin made lethal to tumor cells; CAC, critical aggregate concentration; HAMLET, human a- lactalbumin made lethal to tumor ... ovine and caprine a- LA [24]. The OAcomplex of bovine a- LA was named bovine a- LAmade lethal to tumor cells (BAMLET) [21]. Moreover,it was also shown that a- LA mutants with amino acidreplacements...
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Báo cáo khoa học: Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor doc
... transfection assays with increasing amounts of DNA fragments encoding cyt b5and monitored theformation of androstadienol and DHEA. As shown inFig. 5 the stimulation of DHEA and androstadienolproduction ... activity reached a maximum at a ratio of 12 : 1. Thus, the influence of human cyt b5changes dramatically as the cyt b5/P450c17 ratio varies.Effect of P450red on DHEA and androstadienol synthesisTo ... used as standards, showedelution peaks at 4.70, 2.30 and 2.50 min, respectively(Fig. 2). In both porcine and human assays using preg as a substrate, an additional peak of elution appeared at15...
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Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx
... Dickinson and Co.) was usedto acquire and analyze data. A minimum of at least 10 000cells was analyzed.Computation of specific constitutive activity (SCA)and relative SCA (RSCA)Given that the transfection ... Programma di Ricerca: Lemalattie della tiroide: dalle basi molecolari alla clinica. Ministerodell’Universita`e della Ricerca Scientifica (MURST), Programma diRicerca: Strategie per la valutazione ... del Lavoro, Universita`di Pisa, Pisa, Italy;2Dipartimento di Oncologia, Divisione di Anatomia Patologica, Universita`di Pisa, Pisa, Italy;3Cattedra di Endocrinologia,Fondazione S Maugeri...
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Báo cáo khoa học: Zinc-binding property of the major yolk protein in the sea urchin ) implications of its role as a zinc transporter for gametogenesis ppt
... weight))1at stage 1, which was lessthan half of that in ovary at stage 1, and remained at a similar value to stage 4. Testes at stage 2 were notanalyzed because of a lack of samples. We calculatedthe ... MYP.Statistical analysisData were expressed as the mean ± SEM. Statistical analy-sis was performed using instat software (GraphPad Soft-ware). The normality of the distribution of data wasevaluated ... blot analysisappeared rather broad, probably because largeamounts of unlabeled CFMYP and EGMYP naturallypresent in the gonads formed broad bands and affectedthe shape of the bands of the labeled...
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Báo cáo khoa học: Construction and biological activity of a full-length molecular clone of human Torque teno virus (TTV) genotype 6 pptx
... numbering accord-ingly), and found to be 3748 nucleotides in length. A TATA-box (TATAA) was located at nucleotides 83–87and a poly (A) sequence ATTAAA at nucleotides2978–2983. A GC-rich area was 107 ... 3177–3182.4 Okamoto H, Nishizawa T, Kato N, Ukita M, Ikeda H,Iizuka H, Miyakawa Y & Mayumi M (1998) Molecularcloning and characterization of a novel DNA virus(TTV) associated with posttransfusion ... Miyata H, Tsunoda H, Kazi A, Yamada A, Khan MA,Murakami J, Kamahora T, Shiraki K & Hino S (1999)Identification of a novel GC-rich 113-nucleotide regionto complete the circular, single-stranded...
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