... encoded within its genome its ownnative MnSOD and iron superoxide dismutase (FeSOD). However, an E. coli strain incapable of pro-ducing its own MnSOD and FeSOD, the QC774 strain,has been developed ... hMnSOD and N73S–Q143AhMnSOD mutants obtained by directed evolution, theN73S–C140S–Q143A hMnSOD mutant created bysite-directed mutagenesis, and the parent Q143A hMn- SOD and wild-type hMnSOD, ... applications, and our kinetic and structural studies also provide new insights into the structure–function rela-tionships of human manganese superoxide dismutase. AbbreviationsFeSOD, iron superoxide dismutase; ...
... repair - identifying importantcellular characteristics Summary Fracture repair is a complex and multifactorial process, which involves a well-programmed series of cellularand molecular events ... focused on the left hand side, and the experiments required (right hand side) were adopted from other studies that were performed at the AO Research Institute. ix Mechanical and mechanobiological ... connective tissues, and via cartilage to bone. However, the process can fail and this results in delayed healing or non-union, which occur in 5-10% of all cases. A better understanding of this process...
... Becher MW, Lee MK, Anderson KL, Jenkins NA, CopelandNG, Sisodia SS, Rothstein JD, Borchelt DR, Price DL, Cleveland DW:ALS-linked SOD1 mutant G85R mediates damage to astro-cytes and promotes rapidly ... mimic human disease [1,2,5], and thesehave provided important leads towards understanding themolecular pathology of ALS. Since SOD1 is criticallyinvolved in eliminating superoxide, an undesirablebyproduct ... histopathological studies and draftedthe manuscript. QX participated in analysis of histopatho-logical findings and design of figures and schematics. WSconceived and directed the study, and completed...
... each sample and data wereprocessed using CellQuest software (Becton-Dickinson).Fluorescent emission of propidium iodide and AnnexinV-FITC were collected through a 575 and a 530 ⁄ 30 band-pass ... putativekinase 1 mutations. Mitochondrial and proteasomedysfunction and oxidative stress could account for theselective degeneration of dopaminergic SNpc neurons and their specific vulnerability [1–6].Single ... linear gradient to 8000 V and 2 h and 30 min at 8000 V. IPG strips were then equilibrated for2 · 30 min in 50 mm Tris-HCl (pH 8.8), 6 m urea, 30% glyc-erol, 2% SDS and traces of bromophenol...
... bertini@cerm.unifi.itAbbreviations: SOD, superoxide dismutase; Q133M 2SOD, F50E/G51E/E133Q monomeric mutant superoxide dismutase; FALS,familial amyotrophis lateral sclerosis; M 4SOD, F50E/G51E/V148K/I151K monomeric mutant superoxide ... Nd1of His46, and zinc toNd1 of His63, His71, and His80 and to Od1 of Asp83.Lower and upper distance limits of 1.8 and 2.3 A˚,respectively, were imposed between t he metal i ons and the donor ... Conte and Maria Silvia ViezzoliDepartment of Chemistry and Centro Risonanze Magnetiche, University of Florence, ItalyThe solution structure of homodimeric Cu2Zn2 superoxide dismutase (SOD) ...
... reactive oxygen species, such as the superoxide anion. Superoxide is intrinsically toxic, andsuperoxidedismutase (SOD) provides important cellular protection. However, if the Hb molecule is located ... to eliminatethe superoxide. Chang [2], Alagic [4] and Tarasov [5]have characterized Hb chemically conjugated to SOD alone or SOD combined with catalase, i.e. Hb SOD and polyHb SOD catalase, respectively. ... bottom): the high molecularmass complex, an SOD Hb dimer, and finallyan SOD Hb monomer.ABFig. 3. Reduced SOD Hb and Hb were bubbled with O2(A) or CO(B). SOD Hb shows the absorption spectrum characteristics...
... Urbanit, and the fraction of left-wing parties inparliament, Leftit. Bootstrapped standard errors in brackets.∗∗∗,∗∗, and ∗denote statistical significance at the 1-percent, 5-percent, and 10-percent ... ega-litarianism, altruism, spite and parochialism in childhood and adolescence2011-06 Octavio Fern´andez-Amador, Martin G¨achter, Martin Larch, GeorgPeter: Monetary policy and its impact on stock ... Urbanit, and the fraction of left-wing parties inparliament, Leftit. Bootstrapped standard errors are in brackets.∗∗∗,∗∗, and ∗denote statistical significance at the 1-percent, 5-percent, and...
... units) SOD2 –1500 SOD2 –1500/ – 1335 (T)**0120ABC10080604020 SOD2 –2000 SOD2 –1500 SOD2 –1200 SOD2 –1000 SOD2 –500 SOD2 –200 SOD2 –100 SOD2 –0 SOD2 transcriptional activity(Arbitrary units)CTLBet A***##***0BERCcuLTCTGCAGTTCTGCAGTTCTGTAGTBERC0cuLBERC0cuL050100150CTL ... 6G), and HBx proteinexpression (Fig. 6H), whereas a combination of BetA and SOD2 overexpression totally abolished the BetA-mediated HBV-inhibitory effect. On the other hand, SOD2 knockdown (siSOD2) ... levels for CREB, pCREB and SOD2 were measured by western blottingfrom liver tissue and quantitated. (D) Enzyme activities for caspase-3 and SOD2 from liver tissue were measured and expressed as arbitraryunits....
... Nd-sDmutant and two transgenic lines. Proteins from cocoon shells of a normal breedC108 (C; lanes 1 and 5), Nd-sD(ND; lanes 2 and 6), L6 · 7(L6;lanes3 and 7), or L7-4 (L7; lanes 4 and 8) were ... lanes 1and5 ),Nd-sD(200 lg; Fig. 5, lanes 2 and 6) and twotransgenic lines [L6 · 7(5lg; Fig. 5, lanes 3 and 7) and L7-4 (200 lg; Fig. 5, lanes 4 and 8)] were subjected toWestern blotting with ... transgenic lines (L6 · 7 and L7-4), fhx/P25was detected as two bands of 30 and 27 kDa (Fig. 5C, lanes3, 7 and 4, 8) like in the normal breed C108 (Fig. 5C, lanes 1 and 5). The slight decrease...
... ApoA-I and apoA-II in innate immunity of the carp (Eur. J. Biochem. 271) 2989Fig. 4 B, t he inta ct a poA-I (b and a), an intermedia ryfragment (band b) and a more stable third band (band c)were ... T he positive c ontrol containedbacteria and diluent only. P. citreus , Y. ruckeri and Pseudo-monas sp. were incubated at 2 0 °CandE. coli at 37 °Candthe attenuance (D) was read at 570 nm ... procedureinvolving affinity and gel filtration chromatography and were shown to display bactericidal and/ or bacteriostaticactivity in the micromolar range against Gram-positive and Gram-negative bacteria,...
... resulting in a high affinity and low Vmaxatlow sucrose concentrations (Vmax1 and Km1) and lowaffinity and high(er) Vmaxat high sucrose concentra-tions (Vmax2 and Km2) [13]. In Table ... the enzymethat are clearly important for amylosucrase activity, asb strand 7, connecting to the important Asp393 resi-due, and the region close to the salt bridge and thesecondary sucrose-binding ... substrate and products[10,11] have indicated the presence of various import-ant regions inside and outside the active site pocketcharacterized by sucrose and oligosaccharide-bindingsites (SB and...
... Gary Landucci4, Jason T Kimata5 and Julie Overbaugh*1,3Address: 1Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA, 2Program in Molecular andCellular ... position 120 was a K inSIVMneCL8 and an R in SIVMne170, and reverted to a Kin animal J94233 (19/27 sequences). Position 137 was aT in SIVMneCL8 and an I in SIVMne170, and reverted toa T in most ... helped design and oversee that ADCVI assays and provided comments on the manuscript. GL per-formed ADCVI assays. JTK helped direct the initial infec-tion studies and provided input and comments...