and moss e g 2003 temporally regulated expression of lin 28 in diverse tissues of the developing mouse gene expr patterns 3 6 719 726
Transplantation of skeletal myoblast in ischemic heart disease 1
Ngày tải lên :
12/09/2015, 08:16
... Introcoronary infusion Intra coronary sinus Ischemic heart disease Insulin gene enhancer binding protein Vascular endothelial growth factor receptor Left anterior descending artery Lineage markers Left ventricular ... Figure 3. 6 MHC II staining for hSkMs and myotubes Figure 3. 7 The labeling of SkMs by DAPI, BrdU, and lac-z gene Figure 3. 8 Creating and confirming rat model of MI Figure 3. 9 Representative images to ... 55 56 57 58 58 58 59 59 60 60 60 61 61 62 62 62 62 63 63 63 64 64 65 66 67 68 68 68 68 69 70 xiii 2.11 2.12 2. 13 2.14 BrdU staining, and DAPI fluorescence detection 2.10.2 Identification of Myoblasts...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
Ngày tải lên :
18/02/2014, 17:20
... cell (ESC)-mediated transgenesis Briefly, we constructed a transgene driven by the CAG promoter [25] that expresses the b–geo gene [ 26] in the absence of Cre recombinase, but expresses A new method ... site of pCGX, and the direction was verified by enzymatic digestion and sequencing Generation of a transgenic mouse line carrying the CAG–b–geo–tsA58T Ag transgene SalI-digested pCGX harboring the ... mouse line carrying the CAG-bgeo-tsA58T Ag transgene In order to circumvent the problems described above, we developed a new transgenic mouse line expressing tsA58T Ag under the control of a binary...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
Ngày tải lên :
24/03/2014, 04:21
... homologue were examined and specific PCR primers were designed enclosing the coding sequence of the bovine protein To investigate the presence of MUC15 expression in epithelial cells of the human ... change in cell morphology [37 ] These results suggest a mechanism whereby alternative splicing regulates the relative levels of both the receptor and its secreted cognate binding protein, generated ... ancestral gene [34 ] Despite the location of the MUC15 gene close to the cluster of mucin genes, it does not show the characteristics of this group of secreted gelforming mucins and therefore presumably...
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cancer cell culture, methods and protocols
Ngày tải lên :
10/04/2014, 11:11
... the cell line but to check for the presence of contamination with other cell lines Other levels of characterization include the monitoring of the general features of the cell line and these include ... is therefore worth reconsidering the merit of developing new cell lines if cell lines are readily available The advantages of using existing cell lines include their instant availability and, ... secondly the percentage of cells in the differing stages of the cell cycle (G0 ,G1 ,S ,G2 /M) 6. 5 Summary The above general features of cell lines are too nonspecific to be used to define a cell line but...
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cell and tissue culture
Ngày tải lên :
10/04/2014, 11:12
... Cell line Vero Myeloma cell lines Hybridomas Cell line MRC-5 Cell line WI -38 Cell line Namalwa Cell line BHK-21 Cell line MDCK Cell line GH3 Cell line 2 93 Cell line !VCRE/!VCRIP References 1 .3 Master ... Overview References 1.2 Cell Lines for Biotechnologists Introduction Cell line CHO dhfrCell line Sf9 Cell line Schneider-2 Cell lines COS 1/COS Cell line NIH3T3 Cell line HeLa Cell line J558L Cell ... together with the gene( Urlaub et al., 19 86) The gene is using amplified me which also amplifies the cotrans ene of interest flanking the O cells have been used for the production of recombinant...
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introduction to cell and tissue culture
Ngày tải lên :
11/04/2014, 09:48
... "bulkhead" fittings and hardware should be well sealed where they pass through the wall or ceiling Given that there are different needs, depending on the level of tissue culture being done, we will ... or Eliminating Serum 145 Freezing Cells in Serum-Free Medium 1 46 Carrying Cell Lines in Serum-Free Medium 1 46 Setting Up a Serum-Free Growth Experiment 1 46 References Chapter 9: Primary Cultures ... CULTURE: Theory and Technique Jennie P Mather and Penelope E Roberts Page iii Introduction to Cell and Tissue Culture Theory and Technique Jennie P Mather and Penelope E Roberts Genentech Inc South...
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báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf
Ngày tải lên :
18/06/2014, 15:20
... affected ones The method of cell culture here described could help in the establishment of novel experiments to study the role of FBs in the pathogenesis of the mucosal damage and to test new therapies ... consecutive subjects undergoing EGDS and agreeing to the study, were enrolled Twenty CD (9 males and 11 females, median age 41, range 25–55), 11 (5 males and females, median age 40, range 25– 43) ... discrete object whithin the image The minimum sizes in pixels of the objects to be included in the count was previously defined by accurately measuring 12 representative FBs Objects below the minimum...
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báo cáo hóa học: "Fibrillar beta-amyloid peptide Aβ1–40 activates microglial proliferation via stimulating TNF-α release and H2O2 derived from NADPH oxidase: a cell culture study" doc
Ngày tải lên :
19/06/2014, 22:20
... microglial cells were counted and their numbers expressed as percentage of cell number at time '0' The dashed line indicates cell number at time '0' Data are expressed as mean of experiments ± standard ... hours; then microglial cells were counted and their numbers were expressed as percentage of cell number at the start of the treatment, or time'0' The dashed line indicates cell number at time '0' ... Catalase was also effective in decreasing (by 38 %) the Aβ1–40-induced TNF-α release However, none of the treatments completely prevented Aβ-induced increases in TNF-α levels after 24 hours This suggests...
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Báo cáo y học: " Comparative analysis of cell culture and prediction algorithms for phenotyping of genetically diverse HIV-1 strains from Cameroon" pps
Ngày tải lên :
10/08/2014, 05:21
... viruses Larger studies and data sets are needed from these regions to further substantiate these findings and to understand the complexity of the emerging diversity of HIV in this region of high ... also being labor-intensive and time consuming The present investigation was aimed at characterizing genetically diverse HIV-1 strains circulating in Cameroon in terms of co-receptor usage and comparing ... strains (Table 1) A possible explanation for the predominance of R5 tropism observed with the CRF02_AG viruses we studied may be attributed to the subtype A of the env gene segment in these strains...
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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx
Ngày tải lên :
11/08/2014, 00:22
... HIV-1IIIB infection; this inhibitory effect was independent of the effect of the Replens gel alone In addition, PVP-coated AgNPs with or without the Replens gel completely neutralized cell-free and cell-associated ... the genital epithelium, therefore inactivating the ability of the HIV virus to reach the target cells that reside below Thus, when the HIV virus crosses the genital epithelium, it is already inactivated ... lower chambers In the case of the MT-2 cells, half of the media were replenished with the new media and added to the MT-2 uninfected cells every three days The formation of syncytia was monitored...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
Ngày tải lên :
11/08/2014, 20:21
... visible after weeks of cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure ... samples from stimulated ESCs we detected crystal-like structures in the interior of the microspheres These ESC microspheres resemble aggregates consisting of preosteoblasts Our finding of a mineralization ... an intense mineralization in the centre of the spheres (Figure 2) Transmission electron Figure SEM image of the mineralization in the centre of a +DAG spheres after 21 days Handschel et al Head...
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Báo cáo khoa học: " Thermal stability and inactivation of hepatitis C virus grown in cell culture" ppsx
Ngày tải lên :
12/08/2014, 04:21
... absence of any detergents retained high infectivity (Table 3) Taken collectively, these results suggest that each of the detergents, at the tested concentration, is highly effective in eliminating ... immediately after vortex-mixing, rendering it impossible to delineate the kinetics of viral infectivity reduction during the detergent treatment process This finding is reminiscent Song et al Virology ... glutaraldehyde) are far in excess of the ones examined in the current study and, therefore, should be highly efficient in achieving HCV inactivation Detergents are highly efficient at disrupting the lipidenveloped...
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Báo cáo y học: "Calcium deposition in osteoarthritic meniscus and meniscal cell culture" pps
Ngày tải lên :
12/08/2014, 12:20
... was used for the analysis of differential gene expression and gene ontology In the present study, we focused on the differential expression of selected genes that are involved in the biomineralization ... common in the menisci of end-stage OA patients The ages of two OA patients were similar to the ages of two control subjects, and the ages of five among the eight OA patients were below the age of 60 ... relative transcript levels for selected genes and the housekeeping gene GAPDH was performed using the ABI7000 Real Time PCR system (Applied Biosystems, Inc.) TaqMan® Gene Expression assays (Applied...
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Báo cáo y học: "Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts" pdf
Ngày tải lên :
12/08/2014, 12:20
... Examples of the regulated genes and gene groups are listed in Table The list of regulated genes is available at the Arthritis Research and Therapy homepage Here, the genes are presented, in which the ... in later passages (% changes of gene expression ± standard error of the mean) The regression line shows a clear increment in the course of the culture of the cells (red) (b) Changes in gene expression ... same increase of expression changes in percentage could be detected (regressionline in Figures 2a and 2b) Interestingly, the genes that were differentially expressed at later passages were not...
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Báo cáo y học: " Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research" ppsx
Ngày tải lên :
13/08/2014, 23:20
... differences between the models in Table are negligible can be seen if one considers that a model with 46 parameters, taking each point as the start of a line segment, and having a slope going perfectly ... the rates of length growth The difference between successive length measurements was taken from the unsmoothed data and these differences were then smoothed by the 'rsmooth' command of the Minitab ... parameters are the common midpoint value between the two linear segments, and the two slopes of the linear segments Page of 15 (page number not for citation purposes) Theoretical Biology and Medical...
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Cell isolation and expansion using dynabeads
Ngày tải lên :
22/10/2014, 17:44
... recognize the variable region of the primary antibody and thereby disrupt the binding between the primary antibody and the surface marker As these anti-antibodies out-compete the binding between ... surface antigens can lead to clustering of receptors, triggering of signaling pathways (positive or negative signal) or blocking of receptor function Therefore, choosing the right surface markers and ... colleagues made these beads magnetisable and superparamagnetic, meaning they are only magnetic in a magnetic eld Due to this property, the beads can easily be resuspended when the magnetic eld...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
Ngày tải lên :
14/09/2015, 13:38
... provides first evidence for molecular networking between the Stat3 signalling pathway and Oct4 as well as Nanog in controlling stemness in medaka Since these genes are highly conserved in sequence, ... counteracting the signaling of the full length STAT (α) They exert their dominant negative effects by blocking the DNAbinding sites in STAT responsive elements So far, multiple STAT isoforms generated ... diversity in the recognition of cognate octamer motifs and in regulating expression of target genes Notably, Oct4 has been found to be functionally important in regulating other genes expressed...
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Phytohormones on cell growth and taxol accumulation via cell culture of taxus wallichiana zucc
Ngày tải lên :
23/10/2015, 15:38
... using selection and screening of plant cell cultures have been described by Berlin and Sasse (1985) Cell cloning methods provide a promising way of selecting cell lines yielding increased levels ... combination chemotherapy, are ongoing Paclitaxel is one of the leading anticancer agents in the clinic and the largest-selling anticancer drug of all time In 2000, worldwide sales of Taxol® increased to ... Tuleke These early studies investigating the organogenetic potential of Taxus sp microspores were followed up by Lepage and Degivry who published several papers related to the germination of...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
Ngày tải lên :
05/09/2013, 09:08
... (Stoveland and Lester, 1980; Lester, 19 83) Various flocculating agents involving organic and inorganic compounds are widely used in wastewater treatment, dredging, and fermentation industries However, ... 16. 8 mg of pronase-digested extracellular polymer was obtained from 40 mg of the intact polymer Figure 1B shows the elution profile of the pronase-digested polymer obtained using a Bio-Gel P-100 ... and protein content in the gel filtration - 292 - Journal of Water and Environment Technology, Vol .3, No.2, 2005 eluate were determined by measuring the absorbance at 260 nm and 280 nm, respectively...
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Personality Traits and Workplace Culture
Ngày tải lên :
26/09/2013, 14:10
... contains a listing of the organizational culture traits measured at the ends of the scale, each of them being assigned a value (1 or 3) in parentheses Because the "low" and "high" values used by the ... bottom line, while also engaging in meaningful strategic planning) • • Is the vision based on the values of the founders (i .e past), the current environment (present) or an estimation of the future? ... environment to be able to deliver the expected results for the organization The following table offers the means to assess the degree of cultural match between an individual personality and an organizational...
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