... of posttranslational modifications of plasma-derived and recombinant human HCII from CHO cellsandtheir effects on heparin binding MATERIALS AND METHODS Outdated citrated human plasma was a gift ... exclusion assay and the specific HCII productivity (lg per 106 cells per day) was calculated Ó FEBS 2002 Treatment of HCII expressing CHO cells with inhibitors of N-glycosylation and tyrosine sulfation ... carboxamidomethylation and tryptic digestion of HCII from plasma and from recombinant CHO cells One to two nanomoles of the purified protein were reduced, carboxamidomethylated and digested with trypsin;...
... contributions HWC and CHL presided over and fully participated in all of the work HWC and CHL conceived and designed the experiments CHL and SWR wrote the paper All authors read and approved the ... intensively studied following their discovery in 1991 DSSCs have been extensively researched over the past decades due to their high energy-conversion efficiency and especially their low production cost ... powders were washed thoroughly with distilled water and 0.1 M HCl and subsequently filtered and dried at 80°C for day To achieve the desired TNT size and crystallinity, the powders were calcined in...
... (ChIP)-chip and ChIP with detection by sequencing (ChIPseq) studies on members of the FOXA family published, specifically on FOXA1 (in MCF-7 and LNCaP cells) [12-14] and FOXA2 (mouse liver and a limited ... through ChIP and sequencing on the SOLiD platform Furthermore, we intended to examine their possible interactions with each other, with FOXA2, andtheir correlation with H3K4me3 and other TFs ... binding and candidate functional single nucleotide polymorphisms (SNPs) Results Overall data analysis For the genome-wide analysis of FOXA1 and FOXA3 binding sites and regions of H3K4me3 in HepG2 cells, ...
... transduced cells in patches and scattered about the parenchyma of the cerebellum (D, E and F) The majority of cells were found in the internal granular layer (E and F) but cells were also found in and ... arrow head in H, I, J and K) DNA staining with bis-benzimide is shown C, G and J and phase contrast of the section in A, B and C, and the section in H, I and J, are shown in D and K, respectively ... of the basal artery (C, D and F) Similar distributions were obtained with vector producing cells (A, B, C and D) and helper cells transfected with pREV-HW1 (E, F, G and H) PMS, perimedian sulcus...
... embryonic stem cells, induced pluripotent stem cellsand then the adult bone marrow derived stem cells, which are well known and best studied for their immune modulatory properties Finally, cells from ... and myeloid cells ILT4 is expressed mainly by myeloid cellsand also has been detected at low levels in NK cells (Borges et al 1997) ILT2 and ITL4 bind to a broad range of MHC antigens, but their ... premixes and analogs and close glucose monitoring, the risks of postprandial hyperglycemia and hypoglycemia are present and long-term complications may still develop 1.1.2 Pancreas and islet...
... soyasaponin and soyasapogenol A and B extracts 38 Fig DNA cell-cycle histograms of control (untreated) cellsand TS-, SG-A-, and SG-B-treated cells for 24, 48, and 72 h, respectively Cells ... (control) cells, reflux (RE) and room temperature (RT) extracts treated cells for 24, 48 and 72 h Cells were fixed in 70% ethanol and stained with PI as described in the Materials and Methods ... Hep-G2 cells treated with soyasaponins I and III extract at the LC50 concentration for 72 h Cells were stained by both PI (red) and FITC (green) Control cells (left panel) are untreated cells and...
... isopropanol, and (c) P-10 film Figure 6.5 Optical micrographs of (a) and (d) pristine PET, (b) and (e) P-1, (c) and (f) P-10 surfaces after exposure to airborne and waterborne E coli respectively, and ... supervisors, Prof K G Neoh and Prof E T Kang, for their heartfelt guidance, invaluable suggestions and profound discussion throughout this work, for sharing with me their enthusiasm and active research ... (f) and after 96 h on P-5%H-Si-HA surface (g) and (h) Figure 5.4 Scanning electron micrographs of PC12 cells without NGF after 96 h culture, on TCPS (a) and (b); on P-5%H-Si-HA surface (c) and...
... thigh region, and used lentiviruses to induce the cells to pluripotent cells These pluripotent cells are studied and characterized The pluripotent stem cells exhibited morphology and genomic markers ... stem cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells Hence, fibroblasts from the pig is taken, and induced into pluripotent cells ... fibroblast cellsand differentiate such cells into cardiac lineages to provide a model to study the development of cardiac cells from IPSCs 14 Literature Review 3.1 Human Embryonic Stem Cells (hESCs)...
... cells as the transformational cells in GBM formation, hence their accurate terminology as glioma stem cells or -initiating cells1 4, 16, 17 Traditionally, the similar identification of these cells ... patient‟s original tumor 1.3 GLIOMA STEM CELLS OR -PROPAGATING CELLS In recent years, the distinction between glioma stem cells, gliomainitiating or –propagating cells has been highlighted Elegant ... decrease in survival and proliferation of colon cancer cells both in vitro and in vivo65 In addition, GSK3β inhibition reduces pancreatic cancer cell survival and proliferation66, and studies conducted...
... fibroblast cells (NF103, P6) 63 Figure 4.11: Confocal laser showing images of PI-stained skin fibroblast cells (a) Cells treated with DMEM lacking FCS (b) Cells treated with DMEM+10% FCS Cells ... of the cells after drug treatment The cells were treated with Fc-8b, Fc-8f and Syn1 Time points of 24 h, 48 h, 72h, were adopted for the study The cells were washed twice with 1X PBS and fixed ... images Cells were harvested on cover slips The cells were treated with Fc-8b, Fc-8f and Syn1 Time points of 24 28 h, 48 h, 72h, were adopted for the study The cells were washed twice with 1X PBS and...
... disinformation and propaganda – Deny service to legitimate computer users – Commit unauthorized intrusions into systems and networks that result in critical infrastructure outages and corruption ... malicious intent • Use their skills to expose security flaws Crackers • Person who violates system security with malicious intent • Have advanced knowledge of computers and networks and the skills to ... employees, and cyberterrorists • Password guessing is a basic attack that attempts to learn a user’s password by a variety of means • Cryptography uses an algorithm and keys to encrypt and decrypt...
... evaluate the predictors of clinical and pathological characteristics in these patients with steatosis andtheir diagnostic values in hepatic fibrosis Patients and methods Patients A total of 204 ... steatosis (83 men and 23 women, mean age: 41.08±10.23 years) and the remaining 98 patients were excluded from hepatic steatosis (79 men and 19 women, mean age 39.4±9.81 years) Their diagnoses were ... activity), and G3 (severe activity) All the sections were blindly and independently assessed by pathologists and the results were processed by the Kappa concordance test The inter- and intra-observer...
... Growth Performance and Sperm Quality of Stress Negative Piétrain Boars andTheir Hybrids with Duroc Kawecka et al (2008), Wysokinska et al (2009), Pokrywka and Tereszkiewicz (2011) and Luc et al (2011; ... Carcass composition and meat quality of crossbreds between F1(LandracexYorkshire) sows mated with LandracexDuroc (omega) and PietrainxDuroc (Pidu) Crossbred Boars Journal of Science and Developement ... 219 Growth Performance and Sperm Quality of Stress Negative Piétrain Boars andTheir Hybrids with Duroc Table Production performance, Least square means (LSMSE) of PiDu and Piétrain boars Variable...
... River andtheir runoff characteristics (Ebise et al (2001, 2003, 2004 and 2006)) Here, pollutants contain organic matter, nutrients, SS and other measured substances Fig - Map of the Yodo River and ... loads, Proc of Environmental and Sanitary Research, (JSCE), 20, 27-38 Ebise, S., Inoue, T and Numabe, A (1993) Runoff characteristics and observation methods of pesticides and nutrients in rural rivers, ... composite sample and analyzed inorganic ions and organic matter The water samples taken at the three points spanning the river during the five flooding-stages were analyzed individually, and the representative...
... seaweed and aquaculture Midori Press, Tokyo, pp 133 - 144 Turner, M G., Gardner, R H and ÓNeill R V (2004) Landscape ecology in theory and practice: Pattern and process, Nakagoshi, N and Hara, ... from the bottom and thus, allowing the recovery of U pinnatifida and other non-encrusting macroalgae Both buoyed reefs were made of wood andtheir length was 1.0 m with 1.0 m width and 16 cm height ... macrophyte flora and infauna in the outer and inner areas of the bay A total of 16 macrophyte species in 2003 and 15 species in 2004 were observed in Line (Table 1) U pinnatifida and crustose coralline...
... (3) where NHi and NOi represent NH4-N and NO2+3-N in the ith layer [g-N/m2], respectively, and Nrice is the rice nitrogen [g-N/m2] The terms radv,NH,i and radv,NO,i are the NH4-N and NO2+3-N inflow ... production and water treatment and a detailed understanding of the nitrogen dynamics in VF constructed wetlands CONCLUSIONS In the experimental pots replicating VF constructed wetlands, a variety ... with growth Assuming Monod-type nitrogen uptake kinetics (Selim and Iskandar, 1981) and ignoring the temperature effects, the NH4-N and NO2+3-N uptake by rice is described as rup , NH ,i = k N ,up...
... and Environment Technology, Vol 9, No.2, 2011 In this study, runoff characteristics of materials such as organics, nutrients and inorganic matters from a citrus grove were investigated, andtheir ... in detail and material balances around reservoirs were considered RESEARCH PROCEDURE Location: Matsuyama region and periphery of the Seto Inland Sea The location of the research area and the distribution ... growth chamber under dark condition, and the temperature was maintained constant between 10°C and 30°C RESULTS AND DISCUSSION Materials runoff characteristics and variation in the reservoir Fig...
... indicator and the basis for calculations of heating and cooling energy demand [1] or for estimating bin data and heating and cooling degree-days at any base [2, 10, 11] Table shows the monthly and ... cooling demands is 28.5% and for Thessaloniki 13.2% Obviously, directly proportional to the energy demand for heating and cooling is the fuel (diesel oil) and electricity consumption and hence ... measures applied to dwellings Energy and Buildings 200, 37, 982-95 Frank Th Climate change impacts on building heating and cooling energy demand in Switzerland Energy and Buildings 2005, 37, 1175-85...