... endotoxininduced mortality andlunginflammation The experimental data show that BDL induces recruitment of PIMs and their depletion or inactivation protects BDL rats from E coli LPS inducedlunginflammation ... investigate the role of PIMs in endotoxin -induced mortality andlunginflammation The data show that PIM depletion as well as inactivation prevents mortality andlunginflammation in LPS-treated BDL rats ... against LPS -induced mortality in BDL rats We delineated role of PIMs by comparing LPS -induced lunginflammationand mortality in BDL rats and GCtreated BDL rats The dramatic finding was 100% and 80%...
... http://respiratory-research.com/content/9/1/79 Figure Lunginflammation caused by P2Y and P2X selective agonist Lunginflammation caused by P2Y and P2X selective agonist Hematoxilin-eosin staining of the lung 24 h after the instillation ... purinoreceptors, we administered PPADS, a Figure ATP -induced lung edema and its mitigation by P2 receptor antagonist ATP -induced lung edema and its mitigation by P2 receptor antagonist A Time ... respectively, the lung water content and the vascular permeability status, our observations indicate that vascular permeability andlung water content, both manifestations of lung inflammation, were...
... smoke -induced lunginflammation in mice, and comparison with roflumilast We next wanted to determine whether inhibition of PDE4B, PDE4D and PDE7A expression in the lung would protect against lunginflammation ... with combined 4B/4D and 7A AONs against cigarette smoke -induced lunginflammation Efficacy of treatment with combined 4B/4D and 7A AONs against cigarette smoke -induced lunginflammation Mice were ... treatment with roflumilast on cigarette smokeinduced lunginflammation Effect of6 Effect of treatment with roflumilast on cigarette smoke -induced lunginflammation Mice were treated with oral vehicle...
... decrease lunginflammationand microvascular permeability [15] The initiation of ventilation at birth with large tidal volumes for 15 minutes causes an acute stretch inducedlung injury and a systemic ... after delivery Lung Processing and BAL Analysis The lungs were weighed, and bronchoalveolar lavage (BAL) was recovered by saline lavage of the left lung[ 24] Tissues from the left lung were snap ... care and use committees at Cincinnati Children's Hospital and the University of Western Australia Antenatal corticosteroids have pleotrophic effects that include inducedlung maturation and decreased...
... Figure Lung expression of ICAM-1, VCAM-1, ET-1 and MCP-1 mRNAs in IL-6-/- and IL-6+/+ during normoxic and hypoxic conditions Lung expression of ICAM-1, VCAM-1, ET-1 and MCP-1 mRNAs in IL-6-/- and ... respectively; NS) Lung expression of IL-6, IL-6-R, and gp130 during normoxia and hypoxia Exposure to hypoxia was associated with a rapid rise in lung IL-6 mRNA and protein levels in wild-type mice Lung IL-6 ... diaphragm, and RVSP was recorded immediately Then, the thorax was opened and the lungs and heart were removed The right ventricle (RV) was dissected from the left ventricle plus septum (LV+S), and these...
... C57BL/6 and BALB/c 86 background strains could both be induced to develop robust allergic lunginflammation Induction of allergic lunginflammation Naïve mice were injected i.p at day and day ... T and B cell compartments, and these are associated with secondary lymphoid tissue factors LTβ and CCL21 Lung allergic inflammation might be considered similar in many respects to chronic inflammation, ... monocytes/macrophages and lymphocytes were enumerated based on morphology and staining and expressed as percentages of total BAL cells [28,29] Histology Frozen lung sections were fixed in 1% formamide-acetone and...
... on lung water content and pulmonary permeability Pulmonary edema was assessed by measuring extravascular lung water content (Qwl/BW) and permeability alterations by measuring bloodless dry -lung ... determination of extravascular lung water content (Qwl/BW) and permeability alterations by the determination of bloodless dry -lung weight (DLW/BW) and of the distribution space in the lungs of 125I-labeled ... lung alterations and mechanical ventilation on pulmonary edema Effect of previous toxic lung injury Extravascular lung water (Qwl) after mechanical ventilation in normal rats (open circles) and...
... could be induced by PMs, rat lung epithelial cells were treated with different concentrations of PMs for day, and Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial ... visualized and photographed by computerized UV densitometer (BioRad) Single strand breakage assay (SSBs assay) using pBR322 DNA To measure PM -induced oxidative DNA damage, single strand DNA breakage ... collected and removed the biological species such as pollen and endotoxin Collected PMs were weighted and resuspended in PBS solution Resuspension took place in an ultrasonic bath for 30 minutes And...
... recovery and protection from reinfection EXCESSIVE innate immune signaling: Death due to inflammation Figure Innate and adaptive immunity in acute and chronic lunginflammation A variety of host and ... neutrophilia, alveolar and interstitial edema, Lafferty et al Journal of Inflammation 2010, 7:57 http://www.journal -inflammation. com/content/7/1/57 lung hemorrhage, and elevated TNF-a and IL-6 expression ... early and advanced CF lung disease, respectively [3] B cenocepacia provokes lung epithelial damage and TNF-a secretion that leads to severe pneumonia and sepsis in CF patients [126,127] Excess inflammation...
... on CS -induced protein oxidation in vitro and apoptosis andlung damage in guinea pigs in vivo Effect of N-acetyl cysteine (NAC) on CS -induced protein oxidation in vitro and apoptosis andlung ... predisposition and the nutritional status of the subject, particularly the vita- Scheme : Mechanism of CS -induced lung damage and prevention by vitamin C Figure Mechanism of CS -induced lung damage and prevention ... inducing protein oxidation, proteolysis and apoptosis both in vitro and in A549 epithelial cells pBSQ also mimics CS -induced inflammation, apoptosis andlung damage in vivo Tracing to the mechanism...
... rats and the abdominal vessels were transected After death, the hearts and lungs were removed en bloc from the thorax Then, the lungs were isolated from the heart, the trachea was cannulated and ... had a significant increase in TNF-a and IL-6 serum levels (p = 0.012 and p = 0.010, respectively) (Figure 2B and 2C) Mechanical ventilation induced NF-B and up-regulated TLR4 but not TLR2 As ... absence of infection, induced up-regulation of TLR4 and down-regulation of IRAK3 and IBa protein levels, resulting in an increase of proinflammatory cytokines levels in the lungs and in the systemic...
... Measurement of fluid content in lung The right lung was carefully excised, and then its wet weight was measured Subsequently, the lung was dried for 24 h at 60° and then its dry weight was measured ... significant fibrosis with obvious damage to lung structure and formation of fibrous bands or small fibrous masses; grade 7, severe distortion of lung structure and large fibrous masses; grade 8, total ... (Biocolor, Belfast, Northern Ireland) Expression of TGF-ß1 in bleomycin-treated lung Mice were sacrificed at dpi, and the lung was dissected out The RNA of the lung was isolated using ISOGEN (Wako...
... expression of IFNγ and CXCR3 ligands in lung tissue of CXCR3 KO and WT mice Effect of CXCR3 deficiency on mRNA expression of IFNγ and CXCR3 ligands in lung tissue of CXCR3 KO and WT mice Panel ... cells and CD4+ T cell infiltration into airways and lungs in CXCR3 KO and WT mice after of exposure Effect of CXCR3 deficiency on CD8+ T cells and CD4+ T cell infiltration into airways and lungs ... inflammationand apoptosis induced by IFNγ and CS were also significantly decreased [14,15] Recent reports demonstrated that CCR6, together with its ligand CXCL20/MIP-3α, was involved in CS-induced...
... for IL-1 , IL-8 and CTGF, 500 ng/μl for EGR1 and 200 ng/μl for CYR61 and 18S), μl of each forward and reverse primer (10 μM for IL-1 , IL-6, IL-8, CYR61 and 18S and μM for CTGF and EGR1), 10 μl ... were not observed in lungs from control fetuses Indicators of lunginflammation TNFα protein levels were not detectable and active NF-κB levels and TGF- mRNA levels within lung tissue were not ... rious and CYR61 EGR1ventilation protein levels in lung tissue following injuEGR1 and CYR61 protein levels in lung tissue following injurious ventilation Lung tissue sections stained for EGR1 and...
... lung fibroblast to regulate collagen synthesis and degradation that ultimately results in lung fibrosis [8,17] Inhibiting activation of L-TGF-β1 could suppress development of silica -induced lung ... of lung fibrosis After L-TGF-β1 was processed to become active form, the active TGF-β1 starts the occurrence and development of lung fibrosis In the mid and late phases of the experimental lung ... and the vG stain was weakly positive for collagen fibers At 21 days after instillation, primarily cellular nodules and fibrotic cellular nodules (Stage I and II) were observed in the silica and...
... sustained pulmonary inflammation in the lungand airway remodeling observed in chronic diseases such as asthma and chronic bronchitis Understanding the resolution process and factors that may ... MD) were housed in pairs and provided food and water ad libitum The rats were provided a 12:12-h light/dark cycle and an environment of 22°C and 30–40% humidity Rats were randomly assigned to each ... of inflammationand GCM Studies have suggested that IL-18 may be associated with acute inflammation in the lung [16] or liver [17] To test whether IL-18 directly affects the resolution of inflammation...
... Acute lung injury represents acute hypoxemic respiratory failure and is associated with pulmonary and non-pulmonary risk factors Interestingly, direct lung injury caused by bacteria and indirect lung ... cytokines in the lungand plasma and finally lunginflammation These effects were absent in TLR9-D mice We propose the TLR9 signalling cascade as an additional pathway to induce pulmonary inflammation ... after CpG-ODN challenge CpG-ODN induced a rapid and robust increase in TNF-α and IL-1β mRNA transcripts in lungs of WT mice (Figure 3A) Densitometry (Figures 3B and 3C) revealed that peak cytokine...
... formalin fixed Lungs were embedded in paraffin, sectioned, and stained with haematoxylin and eosin (H&E) and Periodic Acid Schiff (PAS) To quantify mucin production, we counted airways and determined ... frass induced airway inflammationand hyperresponsiveness in mice Mice were sensitized and challenged mice with GC frass via intratracheal inhalation as depicted in Figure 1A Sensitization and ... airway hyperresponsiveness and mucin production, but are not required for TH2 skewing and IgE production following GC frass treatment GC frass induced airway inflammationand hyperresponsiveness...
... increased risk of lung infections in children and adults Smoke exposure is linked in adults to an increased incidence and severity of asthma, impaired lung function and airway inflammation Exposure ... (A and C), and influenza infected mice (B and D) The right panel (E-H) shows sections from mice that were smoke exposed; these were smoke alone mice (E and G) and smoke + influenza mice (F and ... homing and retention of T cells [47] and is also implicated in smoke -induced lung damage in humans [40] and mice [44], as it stimulates macrophages to release MMP-12 MIG is both made by and is...