and elisa based approaches for autoab measurement
Light sheet based fluorescence correlation and cross correlation spectroscopy for quantitative measurements of bio molecules in live cells
Ngày tải lên :
09/09/2015, 11:19
... published in 1- Anand Pratap Singh , Jan Wolfgang Krieger , Jan Buchholz, Edoardo Charbon, Jửrg Langowski, and Thorsten Wohland; The Performance of 2D Array Detectors for Light Sheet Based Fluorescence ... 3- Anand Pratap Singh and Thorsten Wohland; Applications of Imaging FCS, Curr Opin Chem Biol., 2014 A P Singh and J W Krieger contributed equally to this work iv Acknowledgements Foremost and ... measurements and thanks for arranging everything during DKFZ trip I thank Ms Agata Pernus for sharing protocols and the discussions on cell measurements I thank Dipanjan Bhattachrya for helpful...
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LabelMe: a database and web-based tool for image annotation pdf
Ngày tải lên :
07/03/2014, 23:20
... where object and part polygons have high overlap The object-part score for a candidate label is NO,P /(NP + α ) where NO,P and NP are the number of images in IO,P and IP respectively and α is a ... object and the corresponding recorded polygon 2.5 Distributions of object location and size At first, one would expect objects to be uniformly distributed with respect to size and image location For ... the images Note that for applications where it is important to have uniform prior distribitions of object locations and sizes, we suggest cropping and rescaling each image randomly Extending the...
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Báo cáo khoa học: "Paragraph-, word-, and coherence-based approaches to sentence ranking: A comparison of algorithm and human performance" ppt
Ngày tải lên :
17/03/2014, 06:20
... control for the effect of paragraph information on human sentence rankings 4.1 Materials for approaches the coherence -based In order to test the tree -based approach, we took coherence trees for 15 ... that a simple paragraphbased algorithm that was intended as a baseline performed very poorly, and that word -based and some coherence -based algorithms showed the best performance The only commercially ... follows: cause effect for cause-effect; cause absent effect for violated expectation; condition consequence for condition; elaborating elaborated for elaboration, and source attributed for attribution...
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Knowledge-Based Approaches for Evaluating Municipal Bonds doc
Ngày tải lên :
22/03/2014, 18:20
... styles, and to easily switch back and forth between the different styles The information case -based reasoning system uses to construct a score for an attribute includes: Fuzzy Logic Combination Formulas ... incomplete or partial information In other words, MuniCredit can recommend and construct a particular case -based model with much fewer factors, based on the the risk and reward of information This is ... Case -Based Reasoning and Municipal Bond Credit Quality Based on the availability of the data, the nature of the judgments, and the style of analyst decisionmaking, it would appear that case-based...
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Báo cáo khoa học: "Combining Trigram-based and Feature-based Methods for Context-Sensitive Spelling Correction" docx
Ngày tải lên :
23/03/2014, 20:21
... occurred, and just guesses based on the priors Table shows the performance of the baseline method for the 18 confusion sets Trigrams Mays, Damerau, and Mercer (1991) proposed a word-trigram method for ... compared to known methods, which are based on word trigrams This method also has the advantage that training can be done once and for all, and quite manageably, for all confusion sets; new confusion ... experimented with each of the methods and found Bayes to be among the best-performing for the task at hand This method has been described elsewhere (Golding, 1995) and so will only be briefly reviewed...
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Báo cáo khoa học: "Complementing Word Net with Roget''''s and Corpus-based Thesauri for Information Retrieval" pdf
Ngày tải lên :
31/03/2014, 21:20
... file This information has been collected from the Wall Street Journal corpus and uses part of the corpus for training and the rest for testing Also, it has separate lists for such information as ... using only the corpus -based syntactic-relationbased thesaurus, expansion using only the corpusbased co-occurrence -based thesaurus, and expansion using combined thesauri For each method we also ... and Development in Information Retrieval, pages 61-69 Gerda Ruge 1992 Experiments on linguisticallybased term associations Information Processing and Management, 28(3):317-332 Gerard Salton and...
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dyslexia and inclusion classroom approaches for assessment, teaching and learning
Ngày tải lên :
03/06/2014, 01:55
... education institutions and voluntary associations to develop teaching programmes, and resources for identification and intervention for dyslexia (Reid, 2001; Crombie, 2002) Understanding and Identifying ... this effort Programmes and resources It is important therefore to ensure that class teachers have an understanding of dyslexia and of the strategies and supports that are appropriate and can ... results and the subsequent recommendations for support 10 Understanding and Identifying Dyslexia Information processing It can be suggested that dyslexia is an information processing difficulty Information...
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báo cáo khoa học: " Social networks, work and network-based resources for the management of long-term conditions: a framework and study protocol for developing self-care support" potx
Ngày tải lên :
10/08/2014, 10:23
... include the formal and informal, visible and invisible work that is involved in the engagement, sense-making, and appraisal of illnessrelated practices; they are done both individually and collectively, ... reviews [20,21] (one on information -based interventions and the other on social networks) The evidence from our review of informationbased interventions indicates that informational strategies that ... details Health Sciences Research Group, and Collaboration for Leadership in Applied Health Research and Care (CLAHRC) for Greater Manchester, School for Community Based Medicine, University of Manchester,...
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báo cáo khoa học: "P-loop mutations and novel therapeutic approaches for imatinib failures in chronic myeloid leukemia" pps
Ngày tải lên :
10/08/2014, 22:20
... tyrosine kinase inhibitors: review and recommendations for harmonizing current methodology for detecting BCR-ABL transcripts and kinase domain mutations and for expressing results Blood 2006, ... patients taking imatinib compared with 6.3 months for T315I mutations, 10.8 months for M351T mutations, 2.9 months for A-loop mutations and 8.7 months for other mutations [22] Additionally, of the ... nilotinib-resistant mutations at baseline and at progression are higher than those for dasatinib-resistant mutations The P-loop mutations E255K/V, Y253H and F359C/V accounted for 25% (26 of 104) of all baseline...
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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
Ngày tải lên :
12/08/2014, 01:22
... (HC-II) and approved by the US food and drug administration (FDA) The performance characteristics of HC-II assay and the PCR for detection of HPV DNA have been compared in cervical lesions and results ... and consistent study for OSMF and OSCC, in which viral integration and the expression of viral oncogenes (E6 and E7) have been shown [8] HPV detection in potentially malignant and malignant oral ... 94°C for followed by 34 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s and extension at 72° C for min, which was extended for at the final cycle Statistical analysis Level of...
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Báo cáo y học: "Network-based approaches for linking metabolism with environment" ppsx
Ngày tải lên :
14/08/2014, 21:20
... environment is referred to as the seed set, and is represented here as red circles Seed metabolites form the interface between the environment and the metabolic system and link the metabolic habitats of ... tightly linked together and perform basic metabolic functions, whereas the peripheral modules only interact with few other modules and accomplish relatively independent and specialized functions ... variability of size and diversity of composition On the other hand, obligate parasites like Buchnera aphidicola and those microorganisms, such as archaea, that live in extreme and narrowly defined...
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Developing peptide based approaches for systematic enzyme profiling
Ngày tải lên :
10/09/2015, 15:49
... and Ms Peggy Ler from NMR lab and Mdm Wong Lai Kwan and Mdm Lai Hui Ngee from MS lab A hearty “thank you” to my parents, my brother and Mr Du Xiaonan I sincerely thank them for their understanding ... molecular species and scaffolds are uniformly presented so as to maximize protein/ enzyme–ligand interactions Considerable effort has been expended to develop mild and selective methods for anchoring ... microarray format, and therefore may be used for potential highthroughput characterization and fingerprinting of protease activities In this work, we were interested to know whether FRET -based assays...
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Microfluidics and microarray based approaches to biological analysis 5
Ngày tải lên :
16/09/2015, 17:14
... Chapter for high-throughput screenings of kinase activities, and potentially other enzymatic activities The intercalation of various PEGs between the slide surface and the immobilized ... In addition, a novel fluorescence -based approach for quantitative detection of peptide phosphorylation on chip using fluorescently-labeled anti-phosphoserine and antiphosphotyrosine antibodies ... sensitivity, high specificity and the short incubation time needed to detect phosphorylation, this antibody -based approach presents obvious advantages over other existing methods, and may find wider uses...
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Microfluidics and microarray based approaches to biological analysis 4
Ngày tải lên :
16/09/2015, 17:14
... the array format yet In addition, post translation modifications may be lacking for in vitro synthesized proteins, and new strategies are required for the in vivo synthesis, purification and functionalization ... water, (c) M GuHCl and (d) no treatment for 30 min, and probed FITC-anti-GST 4.3 Summary Findings described here present a new strategy for site-specific protein biotinylation and immobilization ... acetic acid) for hr and 110 Chapter cured at 150 °C for hours The epoxy slides were reacted with a solution of mg/mL avidin in 10 mM NaHCO3 for 30 minutes, washed with water, air dried, and the remaining...
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Microfluidics and microarray based approaches to biological analysis 3
Ngày tải lên :
16/09/2015, 17:14
... incubated for an hour with the corresponding kinase (p60 tyrosine kinase for Figure 3.5 a) and PKA kinase for Figures 3.5 b)), and then detected with FITC-labeled anti-phosphotyrosine and antiphosphoserine ... slides incubated for hours, and probed with the FITC-labeled anti-phosphotyrosine antibody for hr 3.2.1.3 Fluorescent Antibody -Based Detection of Kinase Activity Current array -based kinase assays ... moiety, making it synthetically challenging and not easily accessible In order for kinase assay to be performed efficiently and accurately in a microarray format, the substrates need to be attached...
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Microfluidics and microarray based approaches to biological analysis 2
Ngày tải lên :
16/09/2015, 17:14
... independent and has no electrophoretic bias The gated injector is both time dependant and electrophoretically biased, but allows for injecting more analyte and is therefore appropriate for samples ... of PVP PVPbased coating is much easier to implement for microfabricated systems since the long protocols used for polyacrylamide are avoided, and was subsequently used for the chip -based separations ... oven for hours to cure at a temperature of 90 °C After curing, a tight and irreversible bond was formed between the slabs of PDMS and a closed network was formed Master generation by standard...
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Microfluidics and microarray based approaches to biological analysis 1
Ngày tải lên :
16/09/2015, 17:14
... genes and information about their function, but to understand how the components work together to direct cells and organisms Among the most powerful and versatile tools currently available for ... chemistry and linkers for attaching DNA to the chip Two DNA chip formats are currently widely used, these are the cDNA array format18 and the in situ synthesized oligonucleotides array format.19 ... glass slides and probed them with fluorescently labeled proteins and small molecules to screen for protein-protein and protein-small molecule interaction.52 The microarray format allows for the high...
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In situ forming supramolecules and chitosan based hydrogels for regenerative medicine
Ngày tải lên :
21/05/2016, 23:06
... leaving our lovely home and country All this would not be possible without my loving immediate family For good or for bad, they are the ones who always stand behind me, and let me know that I ... delivery systems for controlled and targeted therapeutic application Chapter provides general information of cancer and cancer treatment strategies The recently cancer treatment based on nanocarrier ... systems for cancer therapy 13 5.1 Passive targeting strategies and recent developments 15 5.2 Active targeting strategies and stimuli-triggered ligand presentation 16 Stimuli-response for...
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