and e2 conformations of the na k pump
Báo cáo Y học: Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase b subunit in the energy coupling mechanism of the Na+ pump ppt
... decrease of the Na+ affinity (at pH 8.2–8.3) and an eightfold increase of the Na+ concentration required for half maximal inhibition Upon further increasing the pH, the Na+ affinity of the mutant ... Na+ and H+ movements across the membrane to the decarboxylation of carboxybiotin The model shows the approximate location of important residues of helix IV, helix VIII and of region IIIa of the ... in the presence of 360 mM NaCl The most dramatic effect of the R389A mutant is a shift of the pH optimum by more than 2.5 pH units to the alkaline compared to the wild-type This shift in the...
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Báo cáo Y học: Properties of the Na+/H+ exchanger protein Detergent-resistant aggregation and membrane microdistribution potx
... concentrations of the fractions, together with the relative amount of Na+ /H+ exchanger present in the supernatant and the pellet [19], it was found that over 80% of the Na+ /H+ exchanger remained in the ... recently shown that the Na+ /H+ exchanger is regulated by MEK-dependent (mitogenactivated protein kinase/extracellular signal-regulated kinase kinase-dependent) specific signaling and that the cytoplasmic ... amount at the 105–110 kDa size The amount of 105–110 kDa protein was reduced and in many instances evidence of aggregation was evident at the top of the gels The amount of aggregate present at the...
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Báo cáo Y học: Inhibition of the SERCA Ca21 pumps by curcumin Curcumin putatively stabilizes the interaction between the nucleotide-binding and phosphorylation domains in the absence of ATP pot
... side of the membrane While in the E2 form the Ca2þ binding sites have translocated across the membrane to the luminal side and are of low affinity Furthermore, in the E1 conformational state, the ... sites and ligands, respectively, and [EL] is the concentration of bound ligand The total concentration of sites can be expressed as N[E]0, the product of total protein concentration ([E]0) and the ... 3A) and ATP (Fig 3B) in the absence and presence of curcumin, using the coupled enzyme assay In Fig 3A, the half-maximal activation of the ATPase by Ca2þ was measured in the absence of and in the...
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Báo cáo khoa học: Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus ppt
... near the N-terminus of helix I of the domain and the three residues in loop L2 contributing to the E3-binding site Backbone dynamics of PSBD The region (Met132 to Ser134) before the start of helix ... programme of mutation was therefore undertaken on a subgene encoding the interface domain of the B stearothermophilus E3 The final version of the solubilized E3int domain contains seven mutations of ... representing the interface domain (residues 343–470) of B stearothermophilus E3 The use of the engineered E3int domain (27 kDa as the dimer) was introduced because the high molecular mass (112 kDa) of the...
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Báo cáo khoa hoc:"Effect of the slow (K) or rapid (k +) feathering gene on body and feather growth and fatness according to ambient temperature in a Leghorn × brown egg type cross" ppsx
... K/ k 12 ± 2.4 + w + k /k 17 ± 3.2 + Periods (weeks) and genotypes w w + + + + K/ k k /k K /k k+ /k+ 6 6 31 ± 9.6 23 ± 6.6 33.8 ± 10.2 43.8 ± 9.9 K/ k – – + 10 w k+ /k+ – – % of feathers 6 10.24 ± 1.24 ... whom the body and feathers of the chick at weeks of age are in competition for arginin and cystein, of which the requirement is more important during the synthesis of feathers Feathering genes and ... h per day Finally 36 males and 48 females, half of the rapid feathering genotype (k+ /k+ or k+ /W) and half of the slow feathering (K/ k + or K/ W) were kept in two group batteries and samples...
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Comparing the cultural and linguistic analysis of the English word “meal” and words relating to it in contrast with Vietnamese equivalents.
... favorite of the spirits in Britain It is, of course, the national drink of Scotland, and Scotch is exported to the rest of the world 3.2.3 Other drinks As well as large amounts of hot drinks such ... served, the diners remove the napkin from their place setting, unfold it and put it in their laps When the diners leave the table at the end of the meal, they place napkin loosely next to their ... drink and talk during the meal in which everything from the heaven to the earth is mentioned The men, especially the husband and the grandfather, sip alcohol and tell their family members what they...
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A study of semantic and pragmatic features of the adjective warm and its vietnamese equivalents
... “Introduction” states the rationale of the study, Semantics can be simply defined as the study of meaning It defines the aims, objectives and the significance, presents the is central to the study of communication ... feeling in the heart, positive or negative serve as the theoretical background for the analysis of the semantic feeling, our angry or kindly feelings, feeling of pride or of humility and pragmatic ... semantic and pragmatic features The researcher investigates the data taken from CA Warm-blooded literary works and their Vietnamese equivalents, examines how they are used and then describes them...
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A contrastive analysis of semantic and pragmatic features of the words denoting birds in english and vietnamese
... recognition of the field And the findings of semantic aspects often borrow the WDBs for not only conveying the denotative as well as analysis of pragmatic aspects of these kinds of words will ... details of meanings expressed by the WDBs that once the WDBs are used, the speaker and the hearer must have This thesis has made a study of the semantic and pragmatic the same image or meaning of these ... the semantic and pragmatic features of the WDBs in English and Vietnamese - Analyse the similarities and differences in using the WDBs to express the emotion, attitude, … in English and Vietnamese...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in coordinating a putative ... coordinated by Glu141, Asp142, 2¢ and 3¢OH of the ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and ... Research, the Canadian Foundation for Innovation, Genome Canada through the Ontario Genomics Institute, GlaxoSmithKline, Karolinska Institutet, the Knut and Alice Wallenberg Foundation, the Ontario...
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Tài liệu NEW AND ORIGINAL THEORIES OF THE GREAT PHYSICAL FORCES doc
... position at the present day The present theories of the transmission of light and sound; of the production of winds, and sun-spots, and of the method of development and dissemination of heat, are ... except in the direction of other spheres These theories throw new light upon the character and extent of the atmosphere of the moon and planets, and the consequent availability of those and other ... rightly understanding the modus operandi of the conveyance, and utilization, of these sun-elements, and the workings of this sun-power The presence of a veritable flood of light, heat, and magnetic...
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Tài liệu STRATIGRAPHY AND AMMONITE FAUNA OF THE VOLGIAN AND BERRIASIAN ROCKS OF EAST GREENLAND ppt
... regards as the equivalent of thc npper part of t h e Portland Beds of southern England If, with ARKELL (1946, pp 24-28), we place the Portland Beds in the upper part of the Lower Volgian, they, the ... rei:orrlerl as 020 and 322 m The 11iffel.enrein altibllrle is douhtlcss duc either to the dip of the rocks or to inaccnracy of the altimeters nsed The fauna here comprised species of Snrifes and small ... Institute map, km south-west of the Nicsen) where a thick series of conglon~cratcsand sandstones outcrops A careful examination by the present writer ol the conntry between the Niesen and the Kigi, both...
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Tài liệu A Dissertation on the Medical Properties and Injurious Effects of the Habitual Use of Tobacco pptx
... men void of understanding; capable of soothing the sorrows of the afflicted, of healing the wounds of the spirit, and assuaging the anguish of a broken heart But how it fulfils these desirable indications, ... except what arose from the prejudices of the ignorant, or the obstinacy of those who are slaves to the practice of it The bare assertion of Deimerbroek, "that it kept off the plague," without a ... pipes with the dried leaves of this plant, and when lighted, they breathe the dark cloud of smoke from their mouth and nostrils, and as it curls around their head, ascending towards heaven, they present...
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Tài liệu The Dynamic Retention Model for Air Force Officers- New Estimates and Policy Simulations of the Aviator Continuation Pay Program doc
... analyst The individual then experiences a positive or a negative shock The value of the shock is unknown in advance to either the officer or the analyst The shock affects the value the individual places ... 1984, and the extension of the basic DRM to take into account the effect of the availability of multiyear contracts to certain classes of Air Force officers Unlike other models, the DRM takes into ... response to the creation of the DRM by Glenn Gotz and John McCall At the time the DRM was originally created it was pushing the limits of the capabilities of even the fastest computers The model...
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Tài liệu Static and Dynamic Analysis of the Internet’s Susceptibility to Faults and Attacks docx
... as the average diameter, K, and DIK of the original networks and df , Kf and DIKf denote the diameter, K, and DIK after 10% of the nodes are removed Results are the average of ten runs (a) and ... diameter after 10% of the nodes have failed Similarly, the DIK ratio is defined as DIKf /DIKo where DIKo is the DIK value of the original network, and DIKf is the DIK value after 10% of the nodes have ... least k different edges? Novel 0.7 0.35 0.6 0.3 0.5 0.45 0.4 0.5 0.25 0.35 0.2 0.4 f (k) f (k) 0.3 0.25 k= 1 k= 2 k= 3 k= 4 k> 4 0.3 0.2 f (k) k= 1 k= 2 k= 3 k= 4 k> 4 0.15 0.2 0.1 0.1 0.15 0.05 k= 1 k= 2 k= 3 k= 4...
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... region of Ab(L1–40) and Ab(L1–42), and expressing and purifying the peptides The procedure is of course not limited to the peptide variants produced in this work (F19P, A21G, E22 G, E22 K, E22 Q and ... rounds of sonication in buffer, and at the end of each sonication step the suspension was centrifuged and the supernatants (labeled S1, S2 and S3) were stored pending analysis The pellet was then ... Isolation of monomeric Ab and kinetic analysis of aggregation E Fig Recombinant and synthetic peptides are highly pure and behave similarly on SDS-PAGE and HPLC Peptides were isolated by SEC and analyzed...
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Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf
... residues and hence does not conflict with the global interpretation of the data With the exclusion of the N-terminal octapeptidyl and C-terminal nonapeptidyl fragments of Glu17, Leu34 and Lys46, the ... interruption of the geometry of the recognition helix for either Antp or the TTF-1 HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal portion of helix III ... unreliable within the framework of the theory This could arise from the very wellknown limitations of MF formalism for the case of internal motions occurring on a timescale similar to that of the overall...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... that the novel fold in the N-terminal modules of the catalytic cores of AEPs and prim–pols is unrelated to that of other known polymerases, whereas the RRM-like fold encompassed by their C-terminal ... the two-metal-ion mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the ... connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... Tissue Kinet 21, 169–182 Saxena, S .K. , Sirdeshmukh, R., Ardelt, W., Mikulski, S.M., Shogen, K & Youle, R.J (2002) Entry into cells and selective degradation of tRNAs by a cytotoxic member of the RNase ... analyzed by MALDI-TOF MS The addition of the protease is considered the zero time point The relative intensities of the peaks from the mass spectra were used to calculate the fraction of Pyr-ONC (M23L), ... characterization of onconase activation (Eur J Biochem 271) 1169 13 Table Thermodynamic parameters of the thermal denaturaturation of the different onconase (ONC) variants at 25 °C and pH 2.0 (CGdm/HCl)½,...
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Tài liệu Báo cáo khoa học: The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB pdf
... Kawasaki, S., Moriguchi, R., Sekiya, K. , Nakai, T., Ono, E., Kume, K & Kawahara, K (1994) The cell envelope structure of the lipopolysaccharide-lacking gram-negative bacterium Sphingomonas paucimobilis ... Fragments of HSQC and HMBC spectra of compound Labels illustrate assignment of the amide linkage between the amino group of Asp and the carboxyl group of GalA residue E Scheme The structures of oligosaccharides ... under the stream of air, acetylated and then analysed by GC in comparison with authentic standards, prepared from the respective monosaccharides with (S)- and (R)-2-butanol For determination of the...
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Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf
... rate of 50 nmÆmin)1 with a time constant of s, step resolution of 0.1 nm, and bandwidth of nm The final spectra were taken from the average of five scans The VARSELEC program was used for the secondary ... difference in the biophysical property between the N-terminal and internal FPs, in the hope of improving our understanding of the role of fusion peptides in the fusion event Structure and membrane ... in analogy to the N-terminal FP such as that of gp41 [22] and of influenza HA2 [23], demonstrating the conformational plasticity of the viral fusion peptides However, the helix population in the...
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