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Báo cáo y học: " Effect of Weight Reduction on Cardiovascular Risk Factors and CD34-positive Cells in Circulatio"
... weight adults, and the colony-forming capacity of these cells is blunted [17, 18] Alterations in endothelial cells and EPC function associated with obesity precede atherosclerosis and thrombosis ... that endothelial progenitor cells and hematopoietic progenitor cells have common precursor, the hemangioblast and both may be subsets of bone marrow-derived progenitor cells expressing CD34 Moreover, ... percentage of cells after the diet to the percentage of cells before the diet) and the percentage of body fat lost by each subject during the study A correlation also exists between CD34+ cells and the...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... cells were suspended with 500 lL MACS buffer and applied onto MACS columns Magnetically selected positive cells and depleted cells were cultured independently as lymphatic endothelial cells and ... dishes and cultured at 33 °C (day in Fig 1) For the initial weeks, primary cells, including both tsA58T Ag-negative cells (primarily fibroblasts) and tsA58T Ag-positive cells, proliferated, and ECs ... receptors in these cells Mesenteric BECs and LECs (Fig 5A,B) were also obtained by the same strategy as illustrated in Figs and 4, and were treated with VEGF-A or VEGF-C (Fig 5C) MAPK and Akt phosphorylation...
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Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc
... that binds to CD40 and enhances adenoviral infection of mouse and human DC and B lineage cells Materials and methods Cell lines Epstein–Barr virus- (EBV-) transformed human B cells were provided ... as human and murine B cells DC, LCL, L10A, Blasts: these labels refer, respectively, to day human DC, EBV-immortalized human B cells, murine B lymphoma L10A cells and murine LPS blasts Cells (105) ... detectable homology to CD40 ligand (data not shown) and that enhances adenoviral infection of CD40-positive cells of both human and murine origin, including DC and B cells resistant to infection...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... FCS and 0.1% pluronic F-68Ò The cells were maintained at a density of 0.5–2.5 · 106 cells mL)1 and passaged every 2–3 days For infections, cells were seeded at a density of 0.3–0.6 · 106 cells mL)1 ... expression of HIV- and FLAG-tagged D2L and D2S receptors in Sf9 cells To achieve this, mAbs directed against gp120 (clone 11/4C) and the FLAG sequence were used, and Fig shows the band pattern visualized ... Anti-gp120 Ig and anti-FLAG Ig identified bands corresponding to proteins with a molecular mass equivalent to 43 kDa and 85 kDa for D2L and 39 kDa and 80 kDa for D2S (Fig 1) No bands were detected...
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Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc
... assigned to the asymmetric stretching of CH3, and the band at 2873 cm)1 to its symmetric mode The bands at 2926 and 2853 cm)1 can be assigned to an asymmetric and symmetric stretching mode of CH2, respectively ... between 1300 and 900 cm)1 arise mainly from phosphate associated with nucleic acids, i.e DNA and RNA The absorption bands at 1245 and 1087 cm)1 are characteristic of asymmetric and symmetric ... cm)1 (amide II) [15] The 1450 and 1400 cm)1 bands arise from the side chain of proteins [15], but the C–H bending vibration of fatty acids at 1467 and 1450 cm)1 [3] and the carboxylate vibration...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
... and WiDr cells COX-1 was highly expressed in HT29 and WiDr cells and weakly expressed in KM20L2 cells COX-2 was detected only in WiDr cells The two terminal PGE2-biosynthetic enzymes, cPGES and ... release and PGE2 generation Control cells and cells transfected with the WT or mutant mIID were stimulated for h with IL-1b to assess [3H]arachidonic acid release (B), PGE2 production (C) and COX-2 ... that by sPLA2-IIA and -V (net 4–5%) (Fig 3A) When cells expressing sPLA2-IID were cocultured with those coexpressing COX-2 and mPGES and then stimulated (transcellular prostaglandin biosynthesis...
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Báo cáo khoa học: Transport of the phosphonodipeptide alafosfalin by the H+/peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells doc
... incubating the cells for 10, 30, 60 and 120 with alafosfalin at a concentration of mM The recovery rates were 93 ± 0.7% (Caco-2) and 96 ± 0.3% (SKPT) after 10 and 81 ± 3% (Caco-2) and 90 ± 0.8% ... [14C]Gly-Sar flux PEPT1 and PEPT2 are expressed in the apical membranes of intestinal (PEPT1) and renal (PEPT1 or PEPT2) epithelial cells Absorption of intact di- and tri-peptides and related mimetics ... determined with an excess amount of unlabeled Gly-Sar (31.6 mM Gly-Sar for Caco-2 cells and 20 mM Gly-Sar for SKPT cells) and subtracted from total uptake rates The results are presented as Dixon plots...
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Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx
... conventional CD4+ and CD8+ T cells, and normal or increased development of CD4+ and CD8+ T cells, which have an activated memory-cell-like phenotype ([8–12], reviewed in [13] and [14]) These cells have ... Itk and i NKT and cd T cells Q Qi et al Fig Involvement of Itk in the development of i NKT and NKT-like cd T cells During T-cell development in the thymus, cd T cells separate from ab T cells ... iNKT and NKT-like cd T cells Itk and i NKT cell development NKT cells are a subset of innate T cells characterized by their expression of the NK1.1 marker along with the ab TCR Although these cells...
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Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx
... NPFF-related peptides from cells and tissues were extracted and purified by RP-HPLC, assessed by radioimmunoassay and identified by using on-line capillary HPLC/nanospray ion trap tandem mass spectrometry ... 94 °C and 30 s at 68 °C PCR products were cloned in pEGFPn3 (Clontech) and sequenced on both strands Tissue extraction Mouse spinal cord tissue Animals were handled in accordance with standard ... neurones should help to understand their role in neurotransmision Thus, we investigated the processing of proNPFFA in SH-SY5Y human neuroblastoma cells and COS-7 (nonneuronal) cells transiently transfected...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... HCC cells- reactive T cells are induced by fusion cells, T cells from healthy donors were stimulated by fusions of DCs from the same healthy donors (HLA-A2+) and the HCC cells (HLA-A2+, WT1+, and ... nonadherent cells were removed and the adherent cells were cultured overnight To generate immature DCs (DCs), the nonadherent and loosely adherent cells were collected on the next day and placed ... penicillin, and 0.1 mg/ml streptomycin DCs alone, the HCC cells alone, an unfused mixture of both DCs and the HCC cells were used as controls T cells were purified with nylon wool and cultured...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... growth factor stimulated and not stimulated cells was calculated and compared for different periods before and after intervention Stem-Kine mobilizes CD34 and CD133 Cells Statistics Differences ... 50 cells circumscribed by spindle shaped cells and were counted by microscope As the number of colonies depends on the number of plated cells, normalization of colony number based amount of cells ... total number of cells counted cells being 30,000 per sample The percentage of CD133 and CD34 positive cells was calculated based on the measured number of leukocytes (CD45-positive cells) Quantification...
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Báo cáo hóa học: " Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients" pdf
... Ltd, Japan); 200 cells were counted T cells sorting The CD4+ and CD8+ T cells from 19 CML cases and 17 healthy individuals were sorted using CD4 and CD8 monoclonal antibody and MACS® Magnetic ... cases, and CD4+ and CD8+ T cells from 19 cases TRBV sjTRECs were determined in PBMCs, CD4+ and CD8+ T Quantitative detection of δRec-ψJα sjTRECs in DNA from PBMCs and sorted CD4+ or CD8+ T cells ... δRec-ψJα sjTRECs and TRBV-BD sjTRECs in sorted CD4+ and CD8+ T cells from CML patients Page of cells from 10 patients The clinical data of the patients are listed in Table Mononuclear cells isolation...
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báo cáo hóa học:" Proliferating and differentiating effects of three different growth factors on pluripotent mesenchymal cells and osteoblast like cells" pdf
... followed by PDGFbb and then BMP-2 The mitogenic effect of the growth factors IGF-I and PDGFbb released from the implant coating on osteoblast like cells and pluripotent mesenchymal cells is also in ... experiments with the osteoblast like cells TV and GS participated in the study design and coordination and helped to draft the manuscript All authors read and approved the final manuscript Acknowledgements ... differentiate to adipocytes [19] or osteoblast like cells [18] and therefore serve as a model for pluripotent mesenchymal cells The potential of these cells to differentiate into the osteoblastic linage...
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REGENERATIVE MEDICINE AND TISSUE ENGINEERING - CELLS AND BIOMATERIALS pot
... Matsuoka and Arinobu Tojo Cells for Regenerative Medicine 21 Chapter Bone and Cartilage from Stem Cells: Growth Optimalization and Stabilization of Cell Phenotypes 23 Jan O Gordeladze, Janne E Reseland, ... mir-199a and mir-26a (both targeting SMAD1), mir-126 (targeting VCAM1 and HOXA9), mir-125b 28 Regenerative Medicine and Tissue Engineering - Cells and Biomaterials (targeting ERBB2), mir-145, and ... with development and treatment of disease states Bone and Cartilage from Stem Cells: Growth Optimalization and Stabilization of Cell Phenotypes 29 Gene and microRNA manipulations and selection...
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Cogeneration of Energy and Chemicals: Fuel Cells pps
... Electrodes; Fuel Cells – Alkaline Fuel Cells: Cells and Stacks; Fuel Cells – Direct Alcohol Fuel Cells: Direct Methanol: Overview; Fuel Cells – Molten Carbonate Fuel Cells: Overview; Fuel Cells – Overview: ... Overview: Introduction; Fuel Cells – Phosphoric Acid Fuel Cells: Overview; Fuel Cells – Proton-Exchange Membrane Fuel Cells: Cells; Fuel Cells – Solid Oxide Fuel Cells: Overview; Overview Further ... F, Cabot PL, and Brillas E, Fuel cells for chemical and energy cogeneration, 47–60, Copyright (2006), with permission from Elsevier membrane sandwiched between an H2-diffusion anode and an O2-diffusion...
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Báo cáo khoa học: "Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium" pdf
... normal and lung cancer cells (Fig 3) In addition to the 2 1 νs(PO3 ) and νs(PO2 ), bands at ∼970 and ∼1,085 cm , respectively, there were also bands observed at 916, 1,029, 1,056, 1,118, and ... band at ∼1,650 cm 1 Table Spectral data and vibrational assignments of normal and lung cancer cells* Normal cells † (NHBE ) Cancer cells (NCI-H358) Cancer cells (NCI-H460) 1,649 1,548 1,456 1,400 ... spectra of lung cancer cells The infrared spectra of lung cancer cells were recorded as shown in Figs 1b and c It is noteworthy that the νs(PO32) and νs(PO2 ), bands at ∼970 and ∼1,085 cm 1, respectively,...
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Báo cáo y học: "Targeting Toll-like receptor signaling in plasmacytoid dendritic cells and autoreactive B cells as a therapy for lupus" pps
... TLR9-expressing cells) includes myeloid and pDCs, macrophages and MZ-B cells in mice, and pDCs, MZ-B cells and memory B cells in humans, which can respond directly and without priming to both single-stranded ... only in B cells and plasmacytoid dendritic cells (pDCs) [36] In mice, in addition to B cells and pDCs, myeloid dendritic cells and macrophages also express TLR7 and TLR9 receptors and can respond ... pathway, activates T cells, and promotes development of myeloid dendritic cells (mDCs) Activated T cells direct isotype switching and affinity maturation in autoreactive B cells dependent on CD40/CD40L...
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Báo cáo y học: "Endogenous and exogenous stem cells: a role in lung repair and use in airway tissue engineering and transplantation" pdf
... population of endothelial cells resistant to bronchiolar and alveolar damages and which is capable of giving rise, not only to Clara cells, but also to type I and type II alveolar cells in vitro was ... bioengineering and transplantation Endogenous and exogenous stem and progenitor cells for lung repair The airway epithelium is subjected to a lifetime exposure by inhaled particles and pathogens ... bronchioles, two types of cells, Clara cells and neuroendocrine cells localized in neuroepithelial bodies possess the ability to proliferate in response to bronchiolar and alveolar damages [16]...
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Báo cáo y học: "Aortic dissection associated with cogans’s syndrome: deleterious loss of vascular structural integrity is associated with GM-CSF overstimulation in macrophages and smooth muscle cells" docx
... areas collocated with clusters of inflammatory cells (Fig 3B and 3C) MMP1 (Fig 3D) and MMP9 (Fig 3E) were strongly expressed at the intima-media border and in deeper areas of the media Interestingly, ... mRNA expression of collagenase (MMP1) and both gelatinases (MMP2 and MMP9) (CSx) compared with controls (ctrl) exceeding elastolysis and collagenolysis and thus a dramatic loss of structural ... activity at the intima-media border and in deeper areas of the media (m: media; i: intima; original magnification: ×100) (MMP1) and both gelatinases (MMP2 and MMP9) compared with controls Zymography...
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báo cáo khoa học: " Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells" potx
... myelomonocytic cells U-937 and THP-1 proliferate in response to MICA and MICB After we detected that MICA and MICB were secreted by U-937 and THP-1 cells, we determined if external MICA and MICB could ... respective Figure Leukemic myelomonocitic cells express and secrete MICA and MICB THP-1 and U937 cells (1 × 107) were lysed, proteins were immunoprecipitated and equal amounts of proteins from the ... MICA and MICB and found that both proteins were expressed in both cell lines (Figure 1A) To determine if the cells secreted MICA and MICB, we cultivated × 103 cells for up to eight days and evaluated...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu