and atrmr1 receptor homology regiontransmembrane domainringh2 motif protein
Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
Ngày tải lên :
07/03/2014, 03:20
... D
1
and D
2
receptor fusion proteins (D
1
–CFP and D
2
–YFP) in
comparison with emission spectra of the cell popula-
tions that co-express dopamine D
1
receptor fusion
protein (D
1
–CFP) and ... D
1
receptor
fusion proteins (D
1
–CFP and D
1
–YFP).
c
Measured in cell
co-expressing two dopamine D
1
receptor fusion proteins (D
1
MUT–
CFP – genetic variants of dopamine D
1
receptor and ... D
1
receptor.
b
Measured in cell co-expressing dopamine D
1
and D
2
fusion proteins (D
1
–CFP and D
2
–YFP).
c
Measured in cell
co-expressing dopamine D
1
and D
2
fusion proteins (D
1
–CFP and
D
2
R1–YFP...
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Báo cáo khoa học: Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck potx
Ngày tải lên :
08/03/2014, 02:20
... I)-X
(6)8)
-Y-X-X-(L or I) and are
found in the intracellular portions of the TCR complex c,
d, e and f chains, as well as in other immunoreceptors
including the B-cell receptor [3] and several Fc receptors
[4,5]. ... without washing.
NMR of His-cTCRf
The protein was resuspended in 500 lLH
2
Oand50lL
D
2
O, and the pH adjusted to 6 with dilute HCl. Standard
1D and 2D NOESY and DQF-COSY experiments were
recorded ... mgg@soton.ac.uk
Abbreviations: ITAM, immunoreceptor tyrosine-based activation
motif; His-cTCRf, histidine-tagged cytosolic TCRf;PTK ,protein
tyrosine kinase; SH2, src homology domain 2; TCR, T-cell receptor.
(Received...
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Tài liệu Báo cáo khoa học: How disorder influences order and vice versa – mutual effects in fusion proteins containing an intrinsically disordered and a globular protein docx
Ngày tải lên :
18/02/2014, 04:20
... n
2
Þ
where [h]
1
and [h]
2
correspond to the measured mean ellip-
ticity values per residue of proteins 1 and 2, respectively,
and n
1
and n
2
correspond to the number of residues of
proteins 1 and 2.
When ... con-
tent of unordered stretches (50–59%) and a low con-
tent in a-helices and b-strands (2–3% and 22–29%,
respectively), whereas GFP is rich in b-strands (46%)
and exhibits a low content in unordered ... indi-
vidual proteins. M, molecular weight markers; TF, total protein frac-
tion; SOL, soluble protein fraction; proteins purified by immobilized
metal affinity chromatography (IMAC).
Ordered and disordered...
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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt
Ngày tải lên :
18/02/2014, 16:20
... dataset
of all protein RNA structures. This kind of method
was proposed by Sipple [20], and has been applied to
protein structure prediction, protein protein and pro-
tein–ligand interactions ... number of groups
for the prediction of protein structures, protein DNA
and protein ligand interactions [18–35], such an
approach has never been applied to protein RNA
interactions.
In the present ... energetic of protein RNA complexes.
Knowledge-based potential functions have been
employed in protein structure prediction [18–27], as
well as in the prediction of protein protein [25,28–30]
and protein ligand...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
Ngày tải lên :
22/02/2014, 04:20
... LPS/IFN-induced
NO production
Protein kinases such as PKA and PKC, and various factors
such as tumor necrosis factor receptor 6 (TRAF6) and
myeloid differentiation protein (MyD88) are reported to
participate ... mitogen-activated protein kinase kinase;
MyD88, myeloid differentiation protein; NF-jB, nuclear factor-kappa
B; NO, nitric oxide; PKA, protein kinase A; PKC, protein kinase C;
PP2A, protein phosphatase-2A; ... Effects of PKA inhibitors (KT-5720 and H8) and PKC inhibitors
(Ro31-8220 and GF109203X) on the enhancement by TS of LPS/IFN-
induced NO production in VSMC (A), and Western blot analysis of
PKCa...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... blue and 30 m
M
dithiothreitol) and then incubated
at 86 °C for 8 min. Typically, cell extracts (5–10 lgof
protein) were separated by SDS/PAGE using the standard
Laemmli system [30] with 13% and ... Western blot analysis of proteins in samples
collected in (A). 5 lgand1 lg of total protein in samples were separated by SDS/PAGE and immunoblotted with anti-UP12 Ig and anti-GroEL Ig,
respectively. ... buffer,
proteins were eluted from the resin with buffer C [0.1
M
Tris/
HCI (pH 8.0), 1% SDS, 2 m
M
EDTA and 20 m
M
dithiothreitol] and analyzed by SDS/PAGE and Western
blotting.
Subcloning and deletion...
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Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot
Ngày tải lên :
22/02/2014, 07:20
... ribosomal protein S6 kinase and acetyl-CoA
carboxylase by AMP-activated protein kinase and protein
phosphatases in isolated hepatocytes
Ulrike Krause*, Luc Bertrand and Louis Hue
Hormone and Metabolic ... the
process of activation of ACC and p70S6K by amino acids is
inhibited by okadaic acid and calyculin A, two inhibitors of
type 1 and 2A protein phosphatase, and by incubation of
liver cells ... activate p70 riboso-
mal protein S6 kinase (p70S6K) and acetyl-CoA car-
boxylase (ACC) involved in protein and fatty acids
synthesis, respectively. In contrast, the AMP-activated
protein kinase (AMPK),...
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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
Ngày tải lên :
06/03/2014, 11:20
... assay, and
that the relationship between protein concentration and
activity was linear (Fig. S1). The protein concentrations
were 1 lm for beryllium fluoride (BeF
3
)
and BeF
4
2)
) inhibi-
tion and ... in b-cells and neurons, SUR2A
in cardiac and skeletal muscle, and SUR2B in smooth
muscle and some neurons [1]. SUR2A and SUR2B are
encoded by splice variants of a single gene, ABCC9, and
differ ... state and thus
increased channel activation.
Experimental procedures
Protein expression and purification
A FLAG tag was inserted into rat SUR2 between Ala1026
and Asp1027. Full-length SUR2A and...
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Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc
Ngày tải lên :
07/03/2014, 00:20
... transthyretin+TLP
sequences, three motifs were identified,
A’–C’. Motif A’ is equivalent to motif A from
the TLP motif set. Motif B’ is similar but
extended in the N-terminal and C-terminal
regions to motif B. Motif C’ ... dublin TLP and zebrafish TLP are found in the flex-
ible portions of strands B and C that protrude towards
the solvent and in the conformation of the long loop
connecting strands D and E [17]. ... C-terminal
regions to motif B. Motif C’ is shorter than
motif C and its location is shifted towards
the N-terminus. Motif D is specific to the
TLP set of proteins. (C) Motifs A’–C’ were
superimposed on the tertiary...
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Báo cáo khoa học: Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata pot
Ngày tải lên :
07/03/2014, 06:20
... (1980) Exita-
tion profiles, absorption and resonance Raman spectra
of the carotenoprotein ovorubin, and a resonance
Raman study of some other astaxanthin proteins. JAm
Chem Soc 102, 6693–6698.
19 ... 40,
175–215.
30 Randall D, Burggren W & French K (1997) Energy
acquisition: feeding, digestion and metabolism. In Eck-
ert Animal Physiology. Mechanisms and Adaptations
(Randall D, Burggren ... quantities of protein uti-
lizing the principle of protein- dye binding. Anal Bio-
chem 72, 248–274.
34 Davis B (1964) Disc electrophoresis. II. Method and
application to human serum proteins. Ann...
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Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artificially introduced cross-links in proteins and protein complexes potx
Ngày tải lên :
07/03/2014, 12:20
... cross-linking and mass spectro-
metry for mapping three-dimensional structures of
proteins and protein complexes. J Mass Spectrom 38,
1225–1237.
7 Wong SS (1991) Chemistry of Protein Conjugation and
Cross-Linking. ... the
structural analysis of proteins and protein complexes
and in large scale analysis of the function of genes
(proteomics) [1].
For mass spectrometric analysis, the proteins and
proteomes under study ... Koster
1
and Luitzen de Jong
1
1 Biomolecular Mass Spectrometry group, Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands
2 Laboratory of Protein Biochemistry and Protein...
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Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx
Ngày tải lên :
16/03/2014, 00:20
... estrogen and androgen,
because use of antagonists to androgen and estrogen
receptors (flutamide and ICI 182,780, respectively),
and letrozol, an inhibitor of the aromatase responsible
for converting androgen ... and then to
estradiol by the P450 aromatase. It has been shown
that approximately 35% of cervical carcinomas express
aromatase [37] and that DHEA binds to the androgen
receptor and estrogen receptors ... R
(2005) Dehydroepiandrosterone inhibits the prolifera-
tion of HUVEC by enhancing the expression of p53
and p21, restricting the phosphorylation of RB, and is
androgen- and estrogen -receptor independent....
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Báo cáo khoa học: Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia coli transthyretin-related protein potx
Ngày tải lên :
16/03/2014, 01:20
... Stability and fibril formation properties of human and
fish transthyretin, and of the Escherichia coli
transthyretin-related protein
Erik Lundberg
1
, Anders Olofsson
2
, Gunilla T. Westermark
3
and ... tetrameric and monomeric
structures.
Thermostability and protein structures
We have analyzed the structures of hTTR (Protein
Data Bank code: 1F41 [12]), sbTTR (Protein Data
Bank code: 1SN2, [16]) and ... C, and D, of the
tetrameric structures were: hTTR, A10–A122, B10–B122, A¢11–
A¢122, and B¢10–B¢122; sbTTR, A10–A122, B10–B122, C11–C122,
and D10–D122; and ecTRP, A4–A114, B4–B114, C5–C114, and
D4–D114.
c
The...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
Ngày tải lên :
16/03/2014, 18:20
... a-helices and 12%
b-turns (Fig. 1). Incubation of the protein with dif-
ferent ligands such as palmitate, caprylic and arachi-
donic acids and retinol in concentrations sufficient to
saturate the protein ... ethanol and embedded in paraffin. For
R. Jordanova et al. Conformation, ligand binding and distribution of nematode protein Ag-NPA-1
FEBS Journal 272 (2005) 180–189 ª 2004 FEBS 187
Conformational and ... nonpolar and com-
pletely isolated from the solvent.
Here we analyze the conformational and functional
properties of Ag-NPA-1 as well as the tissue and cellular
distribution of the protein. The...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
Ngày tải lên :
16/03/2014, 18:20
... extracts a single band was recog-
nized at 43 kDa indicating the presence of the full-
length protein, in mitochondria three bands at about
42, 43 and 44 kDa were detected, while no bands were
visible ... could
assume that in mitochondria the unprocessed protein,
the mature protein and a post-translationally modified
protein might be present. N-terminal sequencing and
analysis of peptides by MS could help ... d-CDes protein was fused
with the C terminus of the green fluorescent protein
(GFP) in the pGFP-C vector the fusion protein
remained in the cytoplasm (Fig. 4C).
Expression studies on the RNA and protein...
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