and actin based motor proteins

Báo cáo khoa học: F1 – Structure and Function of Motor Proteins pot

Ngày tải lên : 23/03/2014, 15:20

... actin dimer and higher polymer spontaneously own catalyzing ability and can catalyze free actin monomer or dimer changing into actin residues (that refer to the G -actin monomers existing in actin ... cargo-binding and/ or regulatory subunit of both motor proteins Our goal was to identify and characterize the binding site of DLC on myo5a Various fragments of myo5a tail and DLC were expressed in E coli and ... were used The expression of PDGF-R and c-Kit, their ligands, MMP-9 and signal transduction pathways PI3/Akt and MAPK were studied by RT-PCT and western blotting and immunocytochemistry The three...

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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

Ngày tải lên : 07/03/2014, 01:20

... [24,27,29,35–38] Actin and ABPs in transcription regulation It is generally accepted that chromatin-remodeling complexes contain actin, actin- related proteins and ⁄ or ABPs Nuclear actin- related proteins ... includes lung and liver [58] Both ABLIM-2 and -3 strongly bind F -actin and colocalize with actin stress ﬁbers The interaction of STARS with ABLIM-2 and -3 was conﬁrmed by coimmunoprecipitation and further ... regulation B Zheng et al Table Role of nuclear actin- binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain Actin- binding protein Targeting sequence...

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Báo cáo y học: "Archaeal phylogeny based on proteins of the transcription and translation machineries: tackling the Methanopyrus kandleri paradox" pdf

Ngày tải lên : 09/08/2014, 20:21

... in two parts: B' and B" The black and white boxes correspond to the B' and B" parts of the gene, respectively S and F represent the split and fusion event hypotheses of the B' and B" parts of ... used to remove from the analysis proteins that have been affected by LGT [18-21] Our recent analyses of bacterial and archaeal phylogenies based on ribosomal proteins showed a minimal occurrence ... time, the position of M kandleri could not be tested, as its genome was not yet available A more recent tree based on a fusion dataset of ribosomal proteins has shown that M kandleri groups with http://genomebiology.com/2004/5/3/R17...

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Interruption in age based and gender based conversationsstudy on types, outcomes and functions = nghiên cứu về thể loại, tác động và chức năng của sự ngắt lời trong đàm thoại trên cơ sở về tuổi và giới

Ngày tải lên : 19/12/2013, 10:39

... Outcomes and Functions of Interruption in Age -based and Genderbased Conversations …………………………………………….… 19 2.1 Types, Outcomes and Functions of Interruption …………………… 19 2.2 Data Analysis and Discussion ... responses and actual situations recorded were compared and analyzed to provide the reliable and valid facts and figures - Data Analysis After collecting data, the researcher summed up and drew ... as by Searle and Vanderveken and Searle, J (1985) This approach, illocutionary logic, evaluates meaning both in terms of truth conditions and success conditions Speech act theory and its critiques...

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Tài liệu Báo cáo khoa học: Restricted localization of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase at the neuromuscular junctions – contribution and expression from motor neurons doc

Ngày tải lên : 18/02/2014, 08:20

... physiological states Both asymmetric and globular forms of AChE are known to be produced by muscle cells [11,12], and the presynaptic motor nerve terminals synthesize and secrete AChE at NMJs [13,14] ... expressed by motor neurons in chick spinal cord is G4 AChE [15] In this article, we analyze the expression and localization of the PRiMA I-linked G4 form of AChE in rat muscles and motor neurons ... data show that both presynaptic motor neuron and postsynaptic muscle ﬁber contribute to the synaptic expression of PRiMA-linked G4 AChE and illustrate its temporal and spatial expression at NMJs...

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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

Ngày tải lên : 18/02/2014, 16:20

... interaction with actin and that GSTC-Vrp1p465)533 binds endogenous actin in yeast lysates and directly binds purified yeast and rabbit skeletal muscle G -actin in vitro (Fig 4B–D and data not shown) ... We also incubated the GST fusion proteins with purified yeast actin or purified rabbit skeletal muscle actin in G -actin buffer and analysed the bound proteins Actin was purified from S cerevisiae ... (GST) fusion proteins as well as GST only in Escherichia coli and incubated beads bearing the purified GST only and GST fusion proteins with crude yeast-cell lysate in G -actin buffer The proteins...

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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modiﬁcations and activity-based analyses doc

Ngày tải lên : 18/02/2014, 16:20

... to further analysis Affinity -based isolation of N-glycosylated proteins is rather simple and is based on lectin-affinity chromatography Lectins are proteins or glycoproteins that recognize oligosaccharides ... Different methods have been used to isolate and identify N-glycosylated proteins and characterize their glycosylation sites In general, glycosylated proteins and peptides are affinity-isolated or chemically ... FSBG-targeted proteins Overlaying an image of the immunoblot with the 2D pattern of silver-stained proteins pointed to candidate ATP-binding or GTPbinding proteins that were selected from the 2D gel and...

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Tài liệu Báo cáo khóa học: A multi-protein complex containing cold shock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA Potential role in mRNA stabilization pptx

Ngày tải lên : 19/02/2014, 12:20

... CSD proteins and these are called dbpB (also known as YB-1, MSY-1, chkYB-1b, EF1A, p50 and FRGY1) and dbpA (MSY4, chkYB-2 and YB2/RYBa) DbpB and dbpA CSD proteins are ubiquitously expressed and ... IL-2 5¢-UTR [31] and the VEGF 5¢-UTR CSD site MSY1, ChkYB-1b and FRGY1 are dbpB/YB-1 proteins MSY-4 and chkYB-2 are dbpA proteins, and MSY2 and FRGY2 are germ cell-speciﬁc CSD proteins lapped ... assay The 44 and V1 RNA probes are derived from pGEM44 and pGEMV1, respectively Cytoplasmic complexes CC44a and CCV1 and unbound RNA probe are indicated (C) Cytoplasmic complexes CC44 and CCV1,...

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Tài liệu Báo cáo khoa học: "An Open Source Toolkit for Phrase-based and Syntax-based Machine Translation" docx

Ngày tải lên : 19/02/2014, 20:20

... including: standard phrase -based decoding, decoding as parsing, decoding as tree-parsing, and forest -based decoding It is easy-to-use and fast A new system can be built using only a few commands To ... Among these are: the phrase -based model, the hierarchical phrase -based model, and the syntax -based models that explicitly use syntactic information on either (both) source and (or) target language ... with all (hierarchical) phrase -based and syntax -based models In particular, our toolkit provides the following decoding modes Phrase -based decoding To fit the phrasebased model into the CKY paring...

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Tài liệu Báo cáo Y học: Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc ﬁnger proteins doc

Ngày tải lên : 22/02/2014, 07:20

... different roles in the activation and repression of transcription [9] In our hands, Sp1 generated a single band and Sp3 generated two bands in EMSAs (two of three bands migrated to the same position; ... The two proteins are found in the same cells and are indistinguishable in terms of DNA-binding speciﬁcity (Fig 5A,B), and both proteins bound independently to the GA motif (Fig 5C) All Sp proteins ... whether Sp1 and Sp3 could bind to the same GA motif, we prepared appropriate GST fusion proteins and performed EMSAs with the P3 probe that included the GA motif Speciﬁc and different shifted bands...

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Tài liệu Sustainable Procurement of Wood and Paper-based Products pdf

Ngày tải lên : 22/02/2014, 09:20

... jacaranda, jacarada cabiuna, jacaranda de Brasil, jacaranda wood, jacaranda-dabahia, jacaranda-preto, jacaranda-rajado, jacaranda-roxo, jacarandaholz, jacaradanda de Brasil, legno di jacaranda, ... and international laws and regulations, and in compatibility with international principles and criteria relevant at the national and sub-national level (FSC Policy and Standards; principles and ... producers and dealers across several Figure Wood and paper -based products have many inputs Energy supply Wood supply Wood and paperbased products Other supplies Water supply Wood and paper-based...

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Tài liệu Onomatopoeia in Spoken and Written English: Corpus- and Usage-based Analysis pot

Ngày tải lên : 24/02/2014, 18:20

... the grammatical and semantic characteristics of onomatopoeia The former includes Kloe (1977), Kaida et al (1985) and Taylor (2006), and the latter Schourup (1993) and Tamori and Schourup (1999) ... corpus- and usage -based analysis to clarify grammatical/semantic features of representative onomatopoeic words; and (iii) to examine register variations of onomatopoeic words Method and Data ... (documents, reports, etc.) / Learned and scientific writings / General fiction / Mystery and detective fiction / Science fiction / Adventure and Western fiction / Romance and love story / Humor) This...

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Advancing Reproductive Health and Family Planning through Religious Leaders and Faith-Based Organizations docx

Ngày tải lên : 05/03/2014, 17:20

... Home -Based Care Strong Partnerships in Kenya Reaching Internally Displaced Persons in Uganda Institutional Capacity Building and Improving Clinic -Based Care 11 Clinic Improvement and ... as female genital cutting and early marriage and childbirth El-Zanaty, Fatma and Ann Way 2006 Egypt Demographic and Health Survey 2005 Cairo, Egypt: Ministry of Health and Population, National ... Ministry of Health (MOH) [Kenya], and ORC Macro 2004 Kenya Demographic and Health Survey 2003 Calverton, Maryland: CBS, MOH, and ORC Macro., 222 communities, and have other programs already in...

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The Breast Cancer Epidemic: Modeling and Forecasts Based on Abortion and Other Risk Factors potx

Ngày tải lên : 06/03/2014, 02:21

... TARs in England & Wales, Scotland, Northern Ireland, Republic of Ireland, Sweden, Czech Republic, Finland, and Denmark; 1968-2006 Table Model Fitting by Country: Regression Intercept and Coefficients, ... incidence is available (2005 for Republic of Ireland; 2004 for England & Wales, Scotland, Northern Ireland, and Sweden; 2003 for Czech Republic and Finland; 2001 for Denmark) Linear Regression Response ... within Ages 45-49 in England & Wales, Scotland, Northern Ireland, Republic of Ireland, Sweden, Czech Republic, Finland, and Denmark; 1943-2005 Cancer registrations in Scotland started in 1960 Rates...

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Báo cáo khoa học: Synthesis and function of ribosomal proteins – fading models and new perspectives pptx

Ngày tải lên : 07/03/2014, 01:20

... identiﬁed messengers coding for proteins involved in cell proliferation (MIF and cenpA), survival (i.e survivin, BI-1, and dad1), and ribosome biogenesis (members of the small and large ribosomal subunits) ... understand the molecular mechanism involved in the growth-associated translational regulation of RP mRNAs, several studies have sought to identify the cis-acting elements and the trans-acting ... ubiquitin fusion proteins: ribosomal protein P1 and actin J Mol Biol 328, 771–778 54 Kirschner LS & Stratakis CA (2000) Structure of the human ubiquitin fusion gene Uba80 (RPS27a) and one of its...

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Reducing ill health and accidents in motor vehicle repair pot

Ngày tải lên : 07/03/2014, 11:20

... both feet inside and the gear disengaged Reducing ill health and accidents in motor vehicle repair of pages Health and Safety Executive Fire and explosion Always treat fuel and flammable materials ... use equipment and machinery correctly Do buy and use reduced-vibration tools wherever possible and maintain them Don’t use machines and equipment unless the correct safeguards are in position (eg guards and ... Reducing ill health and accidents in motor vehicle repair of pages Health and Safety Executive Checklist for safe mechanical repair and servicing Questions you should ask Yes/No Slips and trips Are...

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Báo cáo khoa học: Protein-misfolding diseases and chaperone-based therapeutic approaches pdf

Ngày tải lên : 07/03/2014, 12:20

... T K Chaudhuri and S Paul and non-native states of targeted proteins, but how they discriminate between correctly and incorrectly folded proteins and how they selectively retain and target the ... reticulum (ER), e.g calnexin and calreticulin, BiP and ERp 57 [6], is able to recognize misfolded proteins and help their retention in the ER, allowing only correctly folded proteins to reach the cytosol ... ubiquitinated proteins [4] Proteins associated with some neurodegenerative diseases like AD, PD and HD, are tau ⁄ b-amyloid (Ab), a-synuclein and huntingtin, respectively [8] For AD, PD and CJD a...

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LabelMe: a database and web-based tool for image annotation pdf

Ngày tải lên : 07/03/2014, 23:20

... where object and part polygons have high overlap The object-part score for a candidate label is NO,P /(NP + α ) where NO,P and NP are the number of images in IO,P and IP respectively and α is a ... from a photographer who randomly points their camera and ignores the scene However, most of the images in the LabelMe dataset were taken by a human standing on the ground and pointing their camera ... breadth, and depth of the dataset We showed how to enhance and improve the quality of the dataset through the application of WordNet, heuristics to recover object parts and depth ordering, and training...

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Báo cáo khoa học: "Integrating Graph-Based and Transition-Based Dependency Parsers" docx

Ngày tải lên : 08/03/2014, 01:20

... graph -based and transition -based models, respectively, though without integrating the two models Thus, Nakagawa (2007) and Hall (2007) both try to overcome the limited feature scope of graph -based ... node and spanning all nodes in V , as exempliﬁed by the graph in ﬁgure This is a common constraint in many dependency parsing theories and their implementations 2.2 Graph -Based Models Graph -based ... adjectives, adverbs, adpositions, and conjunctions Given that there is a strong negative correlation between dependency length and tree depth, and given that nouns and pronouns tend to be more deeply...

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Báo cáo khoa học: "Combining Statistical and Knowledge-based Spoken Language Understanding in Conditional Models" pptx

Ngày tải lên : 08/03/2014, 02:21

... two top-level commands At the bottom is a zoomed-in view for the “Flight” sub-network State and state are called precommands State and state are called postcommands States 2, 5, and represent slots ... emitted by a state It uses state-dependent ngrams to model the precommands, postcommands, preambles and postambles, and uses knowledge -based CFG rules to model the slot fillers These rules help compensate ... leaving no hidden variables and resulting in a CRF Here, PAC stands for “preamble for arrival city,” and PDC for “preamble for departure city.” The command prior and state transition features...

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