and acr12 gfp fusion constructs
Báo cáo khoa học: Crystal structures of Nipah and Hendra virus fusion core proteins ppt
... comparison between the NiV F fusion core and SV5 fusion core The NiV F and SV5 fusion cores are represented as gold and blue Ca backbone traces, respectively (C) and (D) Comparison of the end ... outside f, b and c positions in HR1, and three out of 21 nonconservative changes occur at positions other than a and d in HR2 (Fig 1B) Comparison with other fusion proteins and a fusion core model ... HR1 and HR2 interactions, including the e and g positions in HR1 and the a and d positions in HR2, are labeled Residues that are important in the end deep groove in HR1 and HR2 are framed and...
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Báo cáo khoa học: "Multi-Field Information Extraction and Cross-Document Fusion" pdf
... 15 people, and testing on 137 people However, the postfusion evaluation differs from the pre -fusion evaluation After fusion, the system returns one consensus target for each person and thus the ... times each candidate x was extracted: Vote(x) = |C(x) > 0| Each of these methods ranks the candidate targets by score and chooses the top-ranked one The experimental setup used in the fusion experiments ... (Riloff and Schmelzenbach, 1998; Huffman, 1995; Soderland et al., 1995) Seymore et al (1999) use HMMs for information extraction and explore ways to improve the learning process Nahm and Mooney...
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Báo cáo hóa học: " Research Article Subspace-Based Algorithms for Structural Identification, Damage Detection, and Sensor Data Fusion" ppt
... in (21), and that a moving average sequence (εk ) can also be encompassed [12, 22] For handling both known and unknown excitations, the use of input/output identification methods is mandatory ... fixed and the moving pools, and thus in each record j, the measurement vectors (0, j) ( j) and Yk may have different dimensions This setup, Yk usually referred to as multipatch measurements setup and ... coefficient, and each frequency (or sum and difference), appear to behave in a reasonably decoupled manner, and to perform a correct isolation of the parameter which has changed [60] The advantages and...
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Báo cáo khoa học: "Enhanced immune response with foot and mouth disease virus VP1 and interleukin-1 fusion genes" potx
... suriv esaesid htuom dna toof eht gnidocne seniccav AND dimsalp ehT noitaniccav lanoitnevnoc ot evitanretla tneiciffe dna efas a sedivorp noitazinummi AND erutcurts citnehtua eht ni sepotipe lanoitamrofnoc ... esaesid htuom-dna-toof tsniaga eniccav AND A Y eiX ,TZ gnehS ,WE nahC ,WF niS ,CS gnehC ,TH gnoW 31 9471-5471 ,952 ,3991 ecneicS nietorp lariv a gnidocne AND fo noitcejni yb azneulfni tsniaga ... secnahne eniccav AND VDVB fo ycnavujda enikotyC EM snilloC ,J eilnworB ,I nospmohT ,I noriboN 777-567 ,81 ,9991 eniccaV stcepsorp dna ssergorp ,selpicnirp :seniccav desab-ANR dna AND PN ofitseR...
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A 1020 _ A 1020M _ 02 : Standard Specification for Steel Tubes, Carbon and Carbon Manganese, Fusion Welded, for Boiler, Superheater, Heat Exchanger and Condenser Applications
... capable of withstanding expanding and beading without showing cracks or flaws, or opening at the weld When properly manipulated, superheater tubes shall withstand all forging, welding, and bending ... heat, and furnace speed 9.2 For Grade A tubes, hardness test shall be made on specimens from each of two tubes from each lot and shall not have a hardness number exceeding 72 HRB 9.3 Grade C and ... as follows: Tensile and Hardness Requirements 9.1 The term lot for tension and hardness tests applies to all tubes prior to cutting, of the same specified outside diameter and wall thickness,...
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Plasma Physics and Controlled Nuclear Fusion, Miyamoto
... Molecule, and Cluster Beams I Basic Theory, Production and Detection of Thermal Energy Beams By H Pauly 29 Polarization, Alignment and Orientation in Atomic Collisions By N Andersen and K Bartschat ... Physics and Controlled Nuclear Fusion By K Miyamoto Vols 1–26 of the former Springer Series on Atoms and Plasmas are listed at the end of the book K Miyamoto Plasma Physics and Controlled Nuclear Fusion ... and 12 1.3 Fusion Core Plasma Progress in plasma physics has been motivated by the desire to realize a fusion core plasma The necessary condition for fusion core plasmas is discussed in 1.3 Fusion...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... of fusion protein was present in inclusion body (Fig 2B) Figure Maps of N-terminal His-tagged gAd-GLP-1-A fusion protein and gAd-GLP-1-A fusion protein (A) N-terminal His-tagged gAd-GLP-1-A fusion ... adiponectin and the C-terminus of GLP-1-A Compared with native GLP-1, the fusion protein has a modified site and larger molecular size, and may circumvent the rapid inactivation and renal clearance ... gAd-GLP-1-A fusion protein (Fig 4B) The fusion protein without His-tag was gAd-GLP-1-A fusion protein (Fig 1B) Figure Enterokinase cleavage of the N-terminal His-tagged gAd-GLP-1-A fusion protein...
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Tài liệu Báo cáo khoa học: How disorder influences order and vice versa – mutual effects in fusion proteins containing an intrinsically disordered and a globular protein docx
... 20 and 60 Samples were separated on 16% SDS PAGE and stained by Coomassie (A) NTAIL -GFP, NTAIL and GFP (B) PNT -GFP, PNT and GFP M, molecular weight markers; 0Â, untreated samples The framed bands ... then inserted downstream NTAIL, PNT and SIC1 at the NcoI and ScaI sites to obtain pET NTAIL -GFP, pET PNT -GFP and pET SIC1 -GFP These constructs encode for fusion proteins bearing a 14 residues ... Thus, the hydrodynamic values of NTAIL -GFP and PNT -GFP proteins not reect the sum of the Table Apparent Mr of IDPs -GFP, IDPs and GFP derived from SDS PAGE and gel ltration Hydrodynamic radii were...
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Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf
... hemifusion but not complete fusion [32] and with the report that lipid-anchored influenza haemagglutinin promotes hemifusion [33]; both imply that the complete fusion cannot be supported by the fusion ... IFP–wt and its relationship to the secondary structure Lipid mixing measurements on the internal and N-terminal fusion peptides indicate that the IFP has higher fusion activity than N-terminal fusion ... facilitates fusion of both inner and outer leaflets of fusing membranes Comparison between class I and II fusion proteins The crystal structures of soluble domain of two class II fusion proteins...
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Tài liệu Báo cáo khoa học: "Nonlinear Evidence Fusion and Propagation for Hyponymy Relation Mining" pdf
... nonlinear evidence fusion and propagation significantly improve the precision and coverage of the extracted hyponymy data This is one of the technologies adopted in our semantic search and mining system ... Tianjin Science and Technology Pillar Program References E Agirre, E Alfonseca, K Hall, J Kravalova, M Pasca, and A Soroa 2009 A Study on Similarity and Relatedness Using Distributional and WordNet-based ... Conference on Very Large Data Bases (VLDB’2008), pages 538–549, Auckland, New Zealand B Van Durme and M Pasca 2008 Finding cars, goddesses and enzymes: Parametrizable acquisition of labeled instances...
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Tài liệu Báo cáo Y học: Differential response of neuronal cells to a fusion protein of ciliary neurotrophic factor/soluble CNTF-receptor and leukemia inhibitory factor pot
... (GST) -fusion protein, purified by glutathione Sepharose 4B and cleaved from GST by thrombin treatment as described by the manufacturer (Pharmacia, Freiburg, Germany) The fusion proteins gp130-Fc and ... structure of the CNTF/sCNTF-R fusion protein is shown in Fig 1B Expression of CNTF/sCNTF-R fusion protein and interaction with the signal transducing b-subunits gp130 and LIF-R Expression of Hyper-CNTF ... in Laemmli buffer and proteins were separated via SDS/PAGE and blotted onto poly(vinylidine fluoride) membranes Membranes were cut into two pieces below the 62-kDa marker band and phosphorylated...
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Information Fusion in Signal and Image Processing ppt
... to present, on the one hand, the general ideas of fusion and its specificities in signal and image processing and in robotics, and on the other hand, the major methods and tools, which are essentially ... of modeling and describing it The information handled in a fusion process is comprised, on the one hand, of the elements of information we wish to fuse together and, on the other hand, of additional ... will be discussed further in an example in Chapter 10 1.6 Fusion in signal and image processing and fusion in other fields Fusion in signal and image processing has specific features that need to...
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Báo cáo khoa học: "Query-based sentence fusion is better defined and leads to more preferred results than generic sentence fusion∗" pdf
... these questions, by collecting and comparing four different fusions for various pairs of related sentences, both generic and question-based ones, and both intersection and union ones While it seems ... # Id 73 189 109 134 Table 1: Mean sentence length (plus Standard Deviation) and number of identical fusion results as a function of fusion type (n = 550 for each type) follows As our starting ... Participants were randomly assigned to either the intersection or the union condition, and within each condition they first had to produce 25 generic and then 25 question-based fusions In the latter...
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Báo cáo khoa học: Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans potx
... for DC-SIGNR and NGA2-Fmoc for LSECtin (Fig S2) As shown in Fig 1, the Bt and appKd values were 1.72 nmol and 49.4 lm for DC-SIGN, 4.25 nmol and 136.4 lm for DC-SIGNR, and 0.39 nmol and lm for ... exogenous ligands, including viruses (e.g HIV and Ebola) and parasites (e.g Schistosoma), and mediates HIV dissemination [10,22,23] Similarly to DC-SIGN, DC-SIGNR also recognizes endogenous ligands, ... specificities of DC-SIGN and its related receptors, DC-SIGNR and LSECtin to 157 pyridylaminated (PA) glycans, including high-mannose-type and agalactosylated complex-type N-glycans, and blood-antigen-type...
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Báo cáo khoa học: Membrane trafficking of CD98 and its ligand galectin 3 in BeWo cells ) implication for placental cell fusion pot
... folding and for ligand–receptor interactions, and because CD98 is an N-glycosylated protein [29,30], in this study we investigated the effect of BFA on the expression and the function of CD98 and ... oligoneucleotide and small interfering RNA affects both amino acid transport and cell fusion in BeWo cells [15–17] More recently, we have shown that CD98 involvement in the process of cell fusion that ... increased surface expression of this molecule and increased fusion of BeWo cells (a well-established choriocarcinoma cell line that can undergo fusion and morphologic differentiation similar to...
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Báo cáo Y học: Fusion of farnesyldiphosphate synthase and epi-aristolochene synthase, a sesquiterpene cyclase involved in capsidiol biosynthesis in Nicotiana tabacum docx
... FPPS and eAS as for the two fusion enzymes FPPS/eAS and eAS/FPPS and these Km were similar to those reported previously for FPPS and sesquiterpene synthases from other sources Apparently, the fusion ... fragments FPPS-L and eAS-L were digested with BamHI and XhoI and cloned into the plasmid pET32eAS-nsc and pET32FPPS-nsc, respectively, as described above yielding plasmids pET32FPPS/eAS and pET32eAS/FPPS ... vector pET32c Two fused enzymes, i.e FPPS/eAS and eAS/FPPS, were constructed First, fragments FPPS-nsc and eAS-nsc were digested with NcoI and BamHI and separately cloned into pET32c as described...
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Báo cáo sinh học: " Relationship between the loss of neutralizing antibody binding and fusion activity of the F protein of human respiratory syncytial virus" doc
... the F protein and characterized these mutations with respect to expression, neutralizing mAb binding, and fusion activity as previously described [21] in an attempt to better understand the mechanism ... better understanding of the mechanism by which the F protein mediates cell fusion Such studies may provide additional insights into the function of the F protein, and help in the selection and development ... LG, RS, and AD declare that they are employees of Centocor, Inc which provided supported for this work Authors' contributions CL generated reagents and performed the fusion assays PB and ND performed...
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Báo cáo sinh học: " The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment" docx
... Intracellular transport and TGN localization of UL20p mutants and gK Transport and localization of UL20p and gK was further assessed by transient coexpression of gK and UL20p and simultaneous detection ... production and virus-induced cell fusion The results suggest that virus-induced cell fusion mechanisms are not required for cytoplasmic virion envelopment Materials and methods Cells and viruses ... wild-type UL20 gene As previously described [30], and as shown here, the CL49 and Y49A mutations partially complemented virus spread and virus-induced cell fusion caused by syncytial mutations in either...
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Báo cáo sinh học: " Inhibition of Henipavirus fusion and infection by heptad-derived peptides of the Nipah virus fusion glycoprotein" doc
... conceived and contributed to the design and use of heptad derived peptides as fusion inhibitors henipaviruses, designed and carried out all cell -fusion assays, interpreted data, and edited and corrected ... conceived and contributed to design and use of heptad derived peptides as fusion inhibitors for henipaviruses and PEG-linked versions of peptides, provided overall supervision and financial support and ... 2:57 and they were large and somewhat insoluble making synthesis and purification problematic In preparation to evaluate these peptides as potential therapeutic fusion inhibitors against NiV and...
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báo cáo hóa học:" Treatment of stiff thoracic scoliosis by thoracoscopic anterior release combined with posterior instrumentation and fusion" potx
... Figure Standing5radiograph years after surgery Standing radiograph years after surgery micro-trauma and less complications [4-6] While some physicians thought thoracoscopic release and open chest ... flexibility was first suggested and applied clinically by the authors It was used to select the fusion segments and predict the correction after surgery, so that patients and their family could be ... data KDKL conceived of the study, and participated in the design of the study and coordination All authors read and approved the final manuscript 13 14 15 Anand N, Regan JJ: Video-assisted thoracoscopic...
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