...
all ofthe OLP epithelium. The count was performed by two investigators without knowledge of
whether the samples were control or experimental. The total number of basal and suprabasal epithelial ... Negative controls consisted of omission ofthe
primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit
serum instead ofthe primary antibody.
Cell quantification ... OLP
would require an increased immunolocalization of hMSH2. Therefore, the reduced expression of
hMSH2 protein could make the epithelium of OLP more susceptible to DNA mutation, making it prone...
... respectively, of that of HPRT. This low activity
could be explained by the fact that PRTFDC1 has a Gly in the position of
the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at
the position of ... spectrophotometric methods.
Because the enzymatic activity of PRTFDC1
towards hypoxanthine and guanine is only 0.26% and
0.09%, respectively, ofthe activity of HPRT, the role
of the PRTFDC1 in purine metabolism ... activity [10].
The structural characterization of numerous com-
plexes ofthehuman HPRT and several bacterial and
protozoan HPRTs have been undertaken [13–17]. The
structure ofhuman HPRT can...
... per
monomer of poly(dT), showing that the changes were
mainly due to the binding ofthe first DNA, while the
binding ofthe second DNA had less influence
(Fig. 5A). To ensure that the estimation ofthe ... by
mutations on the DNA-binding site of Rad51, suggest-
ing that the L2 loop of Rad51 is not far from the
DNA-binding site. Consistent with this idea, the fluor-
escence ofthe tryptophan inserted in the ... 1).
These results confirm that the residues are close to the
DNA-binding site.
We then examined if these fluorescence changes
occurred by the binding ofthe first or second DNA,
by titrating these...
... encoded another type of KLK11, isoform 2,
despite the presence of exon 1c. The secretory path-
ways of KLK11 isoforms appeared to differ, although
both isoforms were secreted from cells. Hence, the ... show the transcription initiation sites ofthehuman and a white arrowhead shows the transcription initiation site of
the mouse. Dashes show gaps and asterisks show the same nucleotide in the ... mechan-
ism of KLK11 gene expression.
Results
Three different primary transcripts are derived
from thehuman KLK11 gene
We determined the nucleotide sequences of 15 clones
of the PCR products from the human...
... and phylogeny. How-
ever, the nature ofthe connections between these
factors needs to be understood at the level ofthe pro-
tein domain. The global properties ofthe HTFN
topology are partially ... property of the
bHLH domains. Therefore, this seems to be a topo-
logical constrain derived from the evolution of this
family.
Evolution based on domain reusing might explain
the abundance of certain ... TFIIE-a, TFIIE-b
F 57 42% Zn finger domains. It contains the 90% of the
members of nuclear receptor
superfamily (they are Zn fingers
also) ofthe HTFN.
14-3-3 zeta, STAT1a, STAT1b, dCREB, ATF-1,
FTF,...
... cells. The positions ofthe molecular size markers (kDa) are indicated to the left and right ofthe (C) and (D),
respectively, whilst the position ofthe Oct-1 (98 kDa approximately) and the two ... (2002) Characteri-
zation ofthe 5¢ untranslated region of alpha and beta
isoforms ofthehuman thromboxane A2 receptor (TP).
Differential promoter utilization by the TP isoforms.
Eur J Biochem 269, ... (Prm)3
within thehuman TXA
2
receptor (TP) gene that
directs expression of TPb in HEL92.1.7 and HEK293
cells [25]. A schematic ofthehuman TP gene highlight-
ing the positions ofthe previously...
... to the
protein of interest. In the case ofthe rpL32 fragment
or the (GCC)
9
sequence, there are several repeated
ZF5-binding sites within a single oligonucleotide.
Therefore, the affinity of ... (1994)
Identification of a GC element in the promotor of the
murine L32 ribosomal protein L32 gene and the 3¢-ter-
minal region ofthehuman apolipoprotein A-I gene,
capable of binding with murine and human ... Institute of Influenza, Ministry of Health
of the Russian Federation. The first pair of primers
(5¢-GGCCTTCAAGGCATTAAG-3¢,5¢-AAACAAATGG
CCTGTCCG-3¢) spanned a 958 bp 5¢-part ofthe ZF5
coding region; the...
... epitopes located
in the MUC5AC C-terminal cysteine-rich part.
Further localization ofthe epitopes of 45M1,
2-12 M1 and 166M1
In order to further locate the epitopes ofthe hitherto
unmapped 45M1, ... detailed
map ofthe epitopes for the mAbs, where 45M1 recog-
nizes an epitope located in the N-terminal region of
the C-terminal cysteine-rich part of MUC5AC, pre-
sumably in the last CysD domain ofthe ... 5. Mapping ofthe 45M1, 166M1 and 2-12M1 epitopes on the C-terminal cysteine-rich part ofhuman MUC5AC. The upper part of the
figure shows a schematic representation of full-length human MUC5AC...
... subunit of
Pph3p required for the binding of Psy2p, we examined
the sensitivity ofthe YBL046w ⁄ Psy4p deletion strain
to cisplatin. The comparable increased sensitivities to
cisplatin ofthe homozygous ... was the same as that ofthe control
strain transformed with the vector YCplac111 (data
not shown). The YBL046w gene was therefore termed
PSY4 (for platinum sensitivity 4).
The primary target of ... Superose
6 at the same anomalous position of 450 kDa as Dro-
sophila His
6
–R2 (Fig. 3) and human His
6
–R2 [16].
Examination ofthe phenotype of S. cerevisiae
strains carrying a deletion ofthe YBL046w...
... per-
centages ofthe cells in the G0 ⁄ G1, S and G2 ⁄ M phases ofthe cell
cycle after treatment with 20 l
M or 100 lM ATP for 24 h. The gat-
ing used for the quantification ofthe cells in the G0 ⁄ ... completely
reversed the inhibition of LXF-289 cell proliferation
by ATP. Further indirect support for the involvement
of ERK1 ⁄ 2 is derived from the observed translocation
of ERK1 ⁄ 2 to the nucleus. The translocation ... detectable
decrease ofthe p50 form of NF-jB1 in the cytosol after
10 min, which is still visible after 60 min (Fig. 5D).
Simultaneously with the decrease of p50 in the cytosol,
an increase of NF-jB1 p50 in the...
... late steps ofthe process. For example, early in the
NER process, RPA assists TFIIH in the opening of
the DNA helix around the damage site [36]. Further-
more, in the presence ofthe XPA minimal ... sites.
Taken together, these findings suggest that the struc-
tural properties of DNA-damaged substrates, whether
intrinsic or the result of protein binding, function in
the recruitment ofthe XPC–hHR23B ... Binding of XPG induces the release
of XPC–hHR23B, whereas XPF–ERCC1 triggers exci-
sion ofthe damaged DNA and the release of XPA
and TFIIH. Subsequently, the newly formed gap in
the DNA is filled by...
... in front ofthe HSVtk promoter and transfected into HeLa cells.
CAT activity was normalized for transfection efficiency. The values of
the activities are set relative to the activity ofthe clone ... mL of sample. The column was eluted at
0.1 mLÆmin
)1
. Fractions of 0.5 mL were collected after the
void volume ofthe column.
SDS/PAGE and protein identification by MALDI-TOF MS
Samples from the ... identifica-
tion of NF1 by MALDI-TOF MS is based primarily on
peptide mass matches within the conserved DNA binding
domain, we cannot distinguish between the various
isoforms. Furthermore, NF1 exist in the...
... localization in
the ER [19]. We found that the TMD of UGT1A pro-
teins appended to the C-terminus ofthe ectodomain of
CD4 plasma membrane glycoprotein was able to retain
the chimeric protein in the ER ... that disruption ofthe dilysine
motif KSKTH of UGT1A by mutation of lysine to
serine residues or by extending the length of the
cytoplasmic tail to relocate the dilysine from the crit-
ical positions ... together, these data indicated that the
TMD domain of UGT1A was sufficient to retain the
ectodomain of CD4 protein in the ER. Because
the carbohydrate moieties of proteins that are trans-
ported to the...
... of the
15
N-
1
H NOEs were obtained
from the difference of two independent experiments. The
values of error were adjusted for the proper tensor2 analy-
sis, by increasing ofthe errors from the ... further our knowledge with
respect to the mechanisms of action of these proteins,
it is crucial that we define the precise boundaries of
the STI1-homologous domain and compare the struc-
tures of ... characteristics ofthe 275–284 segment of
hHR23B. Because ofthe high quality of our
15
N-HSQC spectra, 54 ofthe 58 protonated backbone
nitrogen atoms were available for relaxation measure-
ments. The values...
... independent of 15d-PGJ
2
stimulation.
Thereafter, the precise site of action of 15d-PGJ
2
was identified by further 5¢ deletion analysis dissecting
the )192 to )154 region of Prm3. Deletion of the
PPARc ... (DR5). These data
provide direct evidence for the role of PPARc in the regulation of human
TP gene expression within the vasculature and point to further critical dif-
ferences in the modes of transcriptional ... 15d-PGJ
2
-suppres-
sion of luciferase expression. On the other hand, either
deletion ofthe latter PPARc (b) and RXR IV half
sites, such as within the )154 subfragment, or dis-
ruption ofthe RXR IV half...