analysis — initial isolation screening characterisation and manipulation of cells other components and storage
Báo cáo khoa học: " Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study" doc
... and pre-emptive isolation of highrisk patients only No discharge screening Conventional culture methods Screening of high-risk patients only No pre-emptive isolation No discharge screening Conventional ... on-admission and discharge screening policy Extension of pre-emptive isolation to all patients Rapid PCR technique for admission screening Systematic on-admission and discharge screening policy ... postintervention status (extension of pre-emptive isolation and introduction of the qMRSA test to the surgical ICU; initiation of qMRSA testing and pre-emptive isolation in the medical ICU) was...
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Cloning, characterisation and functional analysis of horseshoe crab c reactive proteins
... Interactions of recombinant CRP-1 and -2 Comparison of expression efficiencies of rCRP-1 and -2 Interactions of CRPs are enhanced in the presence of calcium, as well as during infection CRP-1 and -2 ... tCRP-2 and tCRP-3, and each consists of several isoforms These exhibit differential binding affinity to various carbohydrate moieties, and have different hemolytic and haemagglutination profiles Of ... hemocytes and hemolymph of the horseshoe crabs 2.1 Primers used in the cloning of CrCRP-1 and 31 2.2 Proteins used for calibration of MALDI TOF MS/MS 51 3.1 Assessing the expression and purification...
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Energy and exergy analysis of particle dispersed latent heat storage system
... making and energy storage Dr.Pohekar is a life member of Solar energy society of India and International society of multi-criteria decision making, and fellow of Institution of Engineers (India) and ... International Journal of Energy and Environment (IJEE), Volume 1, Issue 3, 2010, pp.445-458 The effect of Stefan number on energy storage and exergy storage has also been investigated and the results ... variation of HTF temperature and interface location at any instant can be easily obtained by integrating equations (5) and (6) with respect to X and Fo respectively and subsequent application of the initial...
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GENETIC MANIPULATION OF DNA AND PROTEIN – EXAMPLES FROM CURRENT RESEARCH pptx
... domain of Gal4 of yeast (pGAD424-X) A suspected interacting Genetic Manipulation of DNA and Protein – Examples from Current Research Fig Crystal structure of Tus-Ter complex of E coli and RTP ... interaction between Net1 and Sir2 on one hand and between Net1 and Fob1 on the other, and loads Sir2 near the Ter sites This process, as noted above, causes silencing of rDNA and prevents unwanted ... 2-hybrid based selection of random mutations (described below), and biochemical characterizations of the mutant forms of the Tus protein, many aspects of the mechanism of replication fork arrest...
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Báo cáo khoa học: Modular kinetic analysis reveals differences in Cd2+ and Cu2+ ion-induced impairment of oxidative phosphorylation in liver pot
... modularization of the system of interest and subsequent application of modular kinetic analysis allows identification of molecular targets of Cd2+ and Cu2+ ions In the first application of the analysis, ... conclude that the site of action of Cd2+ and Cu2+ ions must be cytochrome bc1 and ⁄ or COX Effects of Cd2+ and Cu2+ ions on the activity of succinate dehydrogenase Bimodular kinetic analysis showed ... Effect of Cd2+ and Cu2+ ions on the kinetics of the CoQ-reducing and CoQoxidizing modules Effect of Cd2+ on the kinetics of the CoQ-oxidizing module (A) and CoQ-reducing module (B) Effect of Cu2+ on...
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Báo cáo khoa học: Isolation, characterization, sequencing and crystal structure of charybdin, a type 1 ribosome-inactivating protein from Charybdis maritima agg. potx
... Overall structure of charybdin b-Strands are shown in blue and a-helices in red The structural elements are labeled as follows: b-strands 1–10 and helices A–J The N-termini and C-termini of the protein ... Muscari sp., Hyacinthus and Iris [10]), some of them highly active and others inactive If this is the case, the protein that we isolated and studied may be an inactive isoform, and the observed activity ... additional two-stranded b-sheet (strands and 3, Figs and 5), which lies opposite the C-terminal domain This b-sheet is not well conserved among the known RIPs and is missing in the numbering of the structural...
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Báo cáo Y học: Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components ppt
... typical profile of sucrose density gradient centrifugation The 30S and 50S subunit peaks and the fractions analyzed in B are indicated B, Western blot analysis of the respective mrps Feature of newly ... human and mouse proteins predicted from the genome analysis data led to the discovery of proteins homologous to them [17,18] Subsequent analysis suggested the presence of Ypr100w homologues in other ... (YPL013c), MRPS35 (YGR165w) and MRPL51 (YPR100w) failed to grow on YPGE medium and showed slow growth on YPD as in the case of disruptants of most other mrp genes In the case of MRPS8, it was indicated...
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Genetic Manipulation of DNA and Protein – Examples from Current Research Edited by David Figurski potx
... domain of Gal4 of yeast (pGAD424-X) A suspected interacting Genetic Manipulation of DNA and Protein – Examples from Current Research Fig Crystal structure of Tus-Ter complex of E coli and RTP ... interaction between Net1 and Sir2 on one hand and between Net1 and Fob1 on the other, and loads Sir2 near the Ter sites This process, as noted above, causes silencing of rDNA and prevents unwanted ... 2-hybrid based selection of random mutations (described below), and biochemical characterizations of the mutant forms of the Tus protein, many aspects of the mechanism of replication fork arrest...
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Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc
... hydrolysis of 1,3-b-oligoglucosides, the subsite theory of Hiromi [46,47] was employed for construction of a subsite map for the estimation of affinities and number of subsites in the active center of ... model kinetic analysis of the hydrolysis of oligosaccharides with different DP is an effective tool for characterization of subsite structure of the active center of exo-glucanases and glucosidases ... Km and Vmax for hydrolysis of G6G are similar to those of G3G (Tables and 4) Therefore, it may be assumed that the enzyme has a mixed mode of action towards laminarins and that it is capable of...
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scanning probe microscopies beyond imaging. manipulation of molecules and nanostructures, 2006, p.559
... observed, 1.2 Chirality and STM: From 0D to 2D Fig 1.16 Monolayer image and model of (S)ISA (A and C) and (R)-ISA (B and D) DL1 and DL2 indicate lamellae composed of ISA and 1-octanol molecules, ... University of Twente MESAþ Institute for Nanotechnology and Faculty of Science and Technology Department of Materials Science and Technology of Polymers 7500 AE Enschede The Netherlands Pascal ... Laboratory of Biophysics and Surface Analysis School of Pharmacy University of Nottingham Nottingham NG7 2RD UK Shan Zou MESAþ Institute for Nanotechnology and Materials Science and Technology of Polymers...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... infected cells Results A range of cell lines and primary cells were infected with HIV-1 (BaL, NL4.3, RFW strains), HSV-1 (NC-1 strain), CMV (Toledo and Towne strains) and VZV (Schenke strain) and ... VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage of cells infected are ... value of 7.41 and the most reliable gene in of the 10 viral infections Other genes with low sumv values were SDHA (8.91), GAPDH (8.74), TBP (9.06) and EEF1G (9.16), though in the context of HSV-1NC1...
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báo cáo hóa học: " Measuring the ICF components of impairment, activity limitation and participation restriction: an item analysis using classical test theory and item response theory" potx
... conception and design of the study, the analysis and the drafting and revision of the manuscript MJ participated in the conception and design of the study and the drafting and revision of the manuscript ... size of the sample and the nature and number of items as well as having other limitations IRT can overcome many of the problems of CTT but is more difficult to perform and understand [20] and ... measures (Ab-IAP) comprising Ab-I, Ab-A and Ab-P The results for the CTT and IRT analysis are initially reported by construct and then the reliability and validity of final measures are explored together...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... infected cells Results A range of cell lines and primary cells were infected with HIV-1 (BaL, NL4.3, RFW strains), HSV-1 (NC-1 strain), CMV (Toledo and Towne strains) and VZV (Schenke strain) and ... VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage of cells infected are ... value of 7.41 and the most reliable gene in of the 10 viral infections Other genes with low sumv values were SDHA (8.91), GAPDH (8.74), TBP (9.06) and EEF1G (9.16), though in the context of HSV-1NC1...
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Báo cáo hóa học: " Characterisation and Skin Distribution of Lecithin-Based Coenzyme Q10-Loaded Lipid Nanocapsules" ppt
... (CoQ10 and GTCC), and the amount of lipid (CoQ10 and GTCC) was kept at a fixed concentration of 12.5% (w/w) with regard to the total mass of 100 g Briefly, CoQ10 and GTCC were mixed at 60°C, and then ... e.g., type and concentration of lipids and surfactants [34], the viscosity of the lipid phase [24] and homogenisation pressure and cycle time [35], affected the mean particle size of LNCs During ... heating enthalpy change and a high likelihood of a supercooled state The decrease of melting point and CI and the presence of supercooled state were explained by the effect of the nanometre particle...
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Báo cáo nghiên cứu nông nghiệp " Marketing and Policy Analysis in the Post-Harvest and Resource Sectors of Vietnam " pot
... philosophies, grading and quality, and product positioning, branding and packaging Policy Analysis and Marketing Workshop II The final of the CARD-sponsored workshops on "Policy Analysis and Marketing ... teaching and research and develop research skills, and to foster networking and collaboration between The University of Sydney and Vietnamese research and teaching institutions Description of Design ... scanned and converted to PDF format 3.2 Project Outcomes The key outcomes of the project are: • development of an understanding of policy analysis and marketing concepts over two sets of workshops...
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Báo cáo vật lý: "Synthesis, Characterisation and Catalytic Performance of Porous Nafion Resin/Silica Nanocomposites for Esterification of Lauric Acid and Methanol" potx
... Amount of Nafion resin on silica (wt%) (b) Figure 7: Conversion (●), selectivity (○) and yield (▼) of methyl laurate from the esterification of lauric acid and methanol as a function of (a) Nafion ... that of NR/S nanocomposite with a Nafion resin load of wt%, as shown in Table Table 2: Comparison of the effects of NR/S-5 nanocomposite and Nafion resin on the esterification reaction Type of ... adsorptiondesorption isotherm for nanocomposites was isotherm type IV, which is the isotherm for mesoporous structured material (Fig 2) Studies of mesoporous pore structure are always linked to isotherm type...
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Báo cáo lâm nghiệp:"Interaction of initial seedling diameter, fertilization and weed control on Douglas-fir growth over the first four years after planting" ppsx
... were dried, ground, and sent to a lab for analysis of the concentrations of all major and micro nutrients with standard laboratory procedures 2.6.3 Vegetation The percent cover of vegetation in ... sampled except Ca and Mg Fertilization resulted in an increase of N, S, and B and a decrease in concentration of P, Douglas-fir seedling treatments and growth K, Cu, Fe, Zn, and Mn Fertilization ... Rainier, east of the town of Orting, and the other on the western coast of the Puget Sound near the town of Belfair Both sites had been harvested during the summer of 1996; the treatment regimes...
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Báo cáo y học: "Virtual screening, identification and experimental testing of novel inhibitors of PBEF1/Visfatin/ NMPRTase for glioma therap" doc
... further analysis The result of the virtual screening of the dataset is summarized in Table For selection of potential ligands, analysis of ligand protein contacts for top ranking poses of every ligand ... USA Virtual screening Different steps involved in virtual screening are briefly described below: Page of 12 a Selection of ligand library and preparation of ligands and protein Virtual screening ... binding modes of the known and new ligands in NMPRTase; (a) NMN, (b) FK866, and docked binding modes of compounds and (c) and (e) panels indicate the first binding modes of compounds and respectively...
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báo cáo khoa học: " Surface electromyography as a screening method for evaluation of dysphagia and odynophagia" ppt
... justified in cases of diseases of temporomandibular joint and some other disorders For screening purposes, however, simple water swallow tests are sufficient Protocol analysis Protocol analysis includes ... reactions are objective and EMG can provide us with qualitative and quantitative data Figure day, pain score and odynophagiasingle swallow and normal drinking of 100 cc of example of a7)due to tonsillectomy, ... the evaluation of dysphagia but also of odynophagia and in the differential diagnosis in cases of compulsive water drinking, excessive water drinking, the malingering of dysphagia and psychogenic...
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báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc
... the experimental work and the majority of analysis, prepared the tables and figures and the primary drafts of the manuscript, and contributed to finalisation of the text and journal-specific formatting ... both rice and Arabidopsis thaliana, and are distributed non-randomly throughout the genome [30-32] Clustering of R genes is known to facilitate tandem duplication of paralogous sequences and generation ... E: Isolation and mapping of resistance gene analogs from the Avena strigosa genome Theor Appl Genet 2004, 109:713-724 Li J, Xu Y, Fei S, Li L: Isolation, characterisation and evolutionary analysis...
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