... numbers The left inset of (B) shows the spectra ofthe four main peaks obtained bythe diode array detector The right inset of (B) reports the SDS/PAGE of phycobilisomes recorded by scanning the ... 6803, together withthe determination of their molecular mass, has successfully been achieved [14] bythe combined use of HPLC coupled on-line with a mass spectrometer equipped with an electrospray ... calculated by using the Prot Parameter tools in the EXPASY program The SDS/PAGE molecular masses were deduced bythe marker used in the electrophoresis reported in the right inset of Fig 1B Molecular masses...
... interaction withthe fiscal revenues and spending variables The feedback effects further increase or decrease the longerterm impact ofthe policy, providing a quantitative picture of whether the economy ... so, the extensive margin (expanding the labor force).24 The change in the labor supply variables were adjusted bythe macro-labor elasticity of two, which is a middle estimate ofthe ranges The ... underlying the adjusted GI long-term baseline in the years 2022 to 2041 trend to the percentage change in the series applied to the value ofthe previous quarter beginning with 2021 Quarter There...
... Ca2+, as the binding site is created upon the formation of a complex withthe E2 subunit ofthe PDH complex and requires the lipoyl groups of E2 [44] Nevertheless, the calcium-dependence of PDP1 ... Biology 2009, of eutheria) The duplication pattern of post-translational regulators ofthe PDH complex differs from that ofthe complex itself The inactivating phosphorylation ofthe PDH complex ... signal coinciding withthe gene duplication event could provide the rationale for the retention ofthe duplicated gene As the change of localization alters the role of a protein in the cell, it could...
... non-verbal language, the tone of voice, the idea, the cadence ofthe words and the rhythm ofthe sentences and so forth However, this thesis provides a very modest analysis on the success of two commencement ... other six items With ―Coherence‖ the importance ofthe relations between the concepts, in a text words, is comprehended; with ―Intentionality‖ the necessity ofthe leadership ofthe aim and the ... ―Informativity‖ the vitality of getting the new message correctly and being capable of differentiating the order ofthe importance of these messages, with ―Situationality‖, maybe the primary one, the context...
... sites within the MGP coding sequence (Fig 2) Analysisofthe phase of each ofthe xMGP introns located within the coding region revealed that introns and are of phase I while intron is of phase ... this study, we present the molecular organization ofthe rst nonmammalian MGP gene and the functional analysisof its promoter We identied a region within the rst 70 bp ofthe xMGP promoter that ... obtained withthe )70/)36TATALUC was approximately sevenfold higher than the one obtained withthe )134/)36TATALUC, suggesting the presence of negative regulatory elements located in the region...
... China) The cartridge was firstly conditioned with ml of methanol and then equilibrated with ml of water After sample was loaded, the cartridge was washed with 2.0 ml of methanol (30%) The analyte ... The comparison ofthe spectra of quasi-MS/MS and of quasi-MS/MS/MS may yield the quasi-third order precursor ions The quasi-MS4 spectra may be obtained through collision ofthe ions with argon in ... isonaringin We found that themassspectrometry fragmentation behaviors between the two pairs of flavanone 7-glycoside isomers were very different, while the within pair massspectrometry behaviors...
... Lin M: The study on the constituents ofthe roots of Polygonum multiflorum Thunb Zhongcaoyao 1993, 24:115-118 Zhang ZG, Lu TS, Yao QQ: Study on the anthraquinone components ofthe roots of Polygonum ... solid sample prepared according to themethod Stability ofthemethod was determined withthe sample solution after 0, 2, 4, and 12 hours in a single day and for further one and two days Data processing ... Multiflori; HPLC: high performance liquid chromatography; MS: mass spectrometry; THSG: 2,3,5,4′-tetrahydroxystilbene-2-O-b-D-glucopyranoside Acknowledgements The project was supported bythe Faculty...
... low LOD and LOQ with UV detection Hence the UV detection method can be used for conventional analysisof these compounds even without massspectrometry However, the MS LOD of these compounds ... method to determine these components Concerning theanalysisof these compounds, there are a few reports that introduced the strategy for the determination of sildenafil bythe widely used HPLC ... constructed by plotting the average peak areas ofthe standard compounds against the corresponding concentrations The limit of detection (LOD) of UV detection and MS–SIR was evaluated as themass giving...
... improved methodology for the determination of sulfonamide residues in meat Journal ofChromatography A 1159 (2007)233-241 SUMMARY DETERMINATION SULPHONAMIDES RESIDUES IN FOOD BYHIGH PERFOPvMANCE LIQUID ... Dunnbier Gudrun Massmann, Asaf Pekdeger - Quantitative determination of three sulfonamides in environmental water samples using liquidchromatographycoupled to electrospray tandem massspectrometry ... (2004) 2329 Che-Yi Lin, Shang-Da Huang - Application of liquid- liquy microextraction and high performance liquidchromatography for the determination of sulfonamides in water Analytica Chimica Acta...
... using online capillary liquidchromatographycoupledwith tandem massspectrometry (MS/MS) using an LTQ-Orbitrap XL mass spectrometer (Thermo Scientific, San Jose, CA) Thechromatography conditions ... compatible with MS-based shotgun proteomics? 2) What is the composition and extent of contamination by saliva ofthe exudate proteome? 3) Can the differential abundance of protein within exudates ... relative amounts in the whole saliva samples compared to exudate samples To further elucidate the composition ofthe exudate proteome, we compared it to theproteome derived from the epithelial cells...
... peak ascribed to the excess of benzoyl reagent decreased, without the loss ofthe peak areas ofthe analytes Another advantage of using benzoyl chloride is the simple way to stop the derivatisation ... exceed the safe limits (50 mg/kg) at the end ofthe life ofthe experiment 16 Figure 7: Mean of histamine concentrations in tuna samples stored at 4oC by day of storage Figure 8: Mean of histamine ... suggested by Earola et al (1993), could not totally remove the excess of dansyl chloride As a result, low amounts ofthe by- products can still be detected in the chromatogram and the peak of histamine...
... preconditioned with 5.0 mL methanol followed by 5.0 mL acidified water The samples were loaded through the cartridges at a rate of less than 3.0 mL/min The cartridges were then rinsed with 5.0 mL of 15% ... dard solution of these dyes, and their optimum values are listed in Table 2.4 Method validation Quantitative analysis was carried out bythe external standard calibration methodThe calibration ... prepared by appropriate dilution of intermediate mixed standard solutions in water to concentrations between 0.0015 and 10 g/mL The sensitivity ofthemethod was evaluated by estimating the limit of...
... actin in the nucleus has been demonstrated by immunogold staining with partial digestion ofthe surface lamina ofthe nuclear matrix to allow penetration ofthe gold particles into the nuclear ... accordance withthe guidelines ofthe Ministry of Education, Science, Sports and Culture of Japan for the use of laboratory animals Cell fractionation Subcellular fractions of rat liver were ... underneath the inner nuclear membrane and function to maintain the nuclear architecture [3] The majority of vimentin may be bound to the nuclear lamina from the outside the nucleus [18] The presence of...
... All in all, the above analysisof Themes in this speech proves the assumptions made earlier Thematization highlights the implicit ideology ofthe author the thematic concern to the freedom cause ... say Mr Bush is the addresser with absolute power: as the Head ofthe State and as the Commander -in -Chief ofthe Army His authority is unlimited over the nation The audience ofthe speech; meanwhile, ... point of departure ofthe message denoting what the clause is concerned with Rheme meanwhile is the part where Theme is developed and it lies in the remainder ofthe clause Normally, the choice of...
... delivery to theliver and induces susceptibility to atherosclerosis The regulatory effect of KLF4 on SRBI reveals a novel pathway to elucidate the mechanism of SR-BI in the development of atherosclerosis ... of oxLDL on the expression of KLF4, the potential reason may be the deficiency of a corresponding ligand–receptor interaction Here, the changes in the SR-BI response to oxLDL were consistent with ... to the binding element ofthe SR-BI promoter region The ChIP assay was used to detect the binding of KLF4 to the SR-BI promoter The cross-linked protein-DNA complexes were immunoprecipitated with...
... outside ofthe nuage in the cytoplasm ofthe migrating PGC FCS analysis revealed that Olvas GFP diffused with a diffusion constant of 7.0 lm2ặs)1 (Fig 3A) During the measurement ofthe PGC, the 10 ... and to measure the mobility ofthe components in the PGCs The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised for observation by microscopy and ... protein in the HeLa cell [24], suggesting the existence of some interactive cellular partner FRAP analysisofthe nuage revealed the slow recovery of OlvasGFP (Fig 3B) When the whole part ofthe single...
... avoiding the use of highly flammable or explosive gases in the laboratory The oxygen is added either in the spray chamber or using a T-connector before the torch When oxygen is added to the plasma, the ... analysisofthe organic solvent is the preferred methodof analysis, either untreated or simply diluted in a suitable solvent Direct organic solvent analysis demands some ... match the detection capability ofthe ShieldTorch System in high purity matrices This application note deals only withthe use of cool plasmas for theanalysisof organics, and this technique...
... coincides withthe expected molecular massofthe ternary complex ( 110 kDa) The TIPRL peak fractions indicated in the bottom ofthe left panel were fractionated by ion exchange chromatographyThe ... binding ofthe third protein without displacing the one that was previously associated withthe complex was interpreted as an evidence of simultaneous binding to PP2Aca The results of these in ... regulator ofthe rapamycin -sensitive signaling pathway by competing with Sit4 for Tap42 [20] The fission yeast homolog of Tip41 has been characterized as a regulator ofthe activity of type 2A...
... interact withthe antitoxin The possible effects ofthe mutations on the stability and structure ofthe protein were analysed by inmunoblotting and CD, respectively The potential ofthe Kid mutants ... 1A) Substitution of these residues by glycine abolished interactions withthe bases without disturbing the structure ofthe flexible region in which they are located The fact that these substitutions ... semiquantitive assessment ofthe relative levels of toxicity ofthe different mutants was derived by comparing the number of colonies and growth ofthe spots at the different levels of dilution 4984 CD...