... Figure Levels of IL-6 and IL-8 in nasal lavage Levels of IL-6 and IL-8 in nasal lavage (A) Time course of IL-6 (B) Time course of IL-8 (C) Mean levels of IL-6 in nasal lavage when stable and at exacerbation ... Among the panel of cytokines measured in nasal lavage fluid changes were only detected in the levels of IL-6 and Virology All subjects had a negative PCR for picornavirus and the panel of other ... obstruction and less reversibility and are smokers or ex-smokers To investigate the feasibility and safety of developing a modelof COPD exacerbation and to investigate a causative role of virus...
... g; Harlan Nossan; Italy) were housed in a controlled environment and provided with standard rodent chow and water Animal care was in compliance with Italian regulations on protection of animals ... All values in the figures and text are expressed as mean ± standard error of the mean (SEM) of N observations For the in vivo studies N represents the total number of ani- http://respiratory-research.com/content/7/1/137 ... role of leukotrienes in vivo come from the experimental modelof bleomycin -induced lung fibrosis Intratracheal instillation of the antitumour agent bleomycin is the most commonly used animal model...
... Hamburg, Germany) All animal procedures adhered to the animal care guidelines of the Institute for Laboratory Animal Research (Guide for the Care and Use of Laboratory Animals) in accordance with ... pictures and drafted the manuscript GM participated in the design of the study and performed the statistical analysis HTA conceived of the study, and participated in its design and coordination and ... short and thick processes and positive lectin stain For each retina, the mean number of microglial cells Page of from the three fields of each counting position (central or peripheral zone, and...
... Santos L, Sayegh M, Nussenblatt RB, Trentham DE, Hafler DA: Oral tolerance: immunologic mechanisms and treatment of animal and human organ-specific autoimmune diseases by oral administration of ... and erythema limited to the foot and/or ankle; = slight oedema and erythema from the ankle to the tarsal bone; = moderate oedema and erythema from the ankle to the tarsal bone; and = oedema and ... tolerance, we studied an animal modelof collagen -induced arthritis (CIA), in which mice undergo repeated oral administration of CII, and monitored changes in immune cell function and factors associated...
... AK and ERL performed the construction of the adenoviral vectors and analysis in the rabbit modelof AIA and AK wrote the initial draft of the manuscript JN and ZM assisted in the therapeutic analysis ... The animals were acclimatized for three days before experimentation and were fed chow ad libitum and water All animal experiments were conducted in accordance with NIH standards of animal care and ... values shown represent the mean ± standard error of the mean IL-10 of human IL-10 (hIL-10), viral IL-10 Effect(mut.hIL-10) on leukocytic infiltration(vIL-10), and mutant human IL-10 (mut.hIL-10) on...
... individual animals Values are means ± standard error of the mean (p < 0.05 versus baseline) acquisition of data and manuscript preparation MES and RV contributed to manuscript preparation JV and YX ... interpretation of data, and manuscript preparation JV contributed to study design, acquisition of data, analysis and interpretation of data, manuscript preparation, and statistical analysis CJS ... of the animal model and implications for the treatment of autoimmune disease Chin Med Sci J 1991, 6:78-83 17 Myers LK, Rosloniec EF, Cremer MA, Kang AH: Collageninduced arthritis, an animal model...
... contributions GC and JD drafted the manuscript FH, LH, SM, CH, FC, JD and NL participated in the design of the study GC and JD conceived of the study, and participated in its design and coordination ... between monosomy and disomy for the X chromosome Conclusions Stimulations by LPS and PW mitogen of blood cells from male and female prepubescent children showed quantita- Page of tive and qualitative ... syndrome group had a significantly lower median production than females (p < 0.03) Increasing the concentration of LPS increased the median production of IL1 in females and in males (p < 0.036; p...
... contributions GC and JD drafted the manuscript FH, LH, SM, CH, FC, JD and NL participated in the design of the study GC and JD conceived of the study, and participated in its design and coordination ... between monosomy and disomy for the X chromosome Conclusions Stimulations by LPS and PW mitogen of blood cells from male and female prepubescent children showed quantita- Page of tive and qualitative ... syndrome group had a significantly lower median production than females (p < 0.03) Increasing the concentration of LPS increased the median production of IL1 in females and in males (p < 0.036; p...
... anti-human iNOS antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and used at a concentration of μg/ml The primary antibodies were incubated overnight at 4°C (anti-TGF-β1 and ... plot of NO2-/NO3- in relation to active and total TGF-β1 in urine of female (Panel A & B) and male (Panel C & D) rats The different dots (circle, triangle, square and diamond) represent values of ... known for its anti-neoplastic and immunosuppressant properties, that is used clinically for malignancy, bone marrow transplantation, and multiple sclerosis A prominent side effect of CYP is hemorrhagic...
... similarities and differences in CXCR2 and its ligands between rats and humans [50] Humans express IL-8 and two IL-8 receptors, CXCR1 and CXCR2, whereas rats not express an IL-8 ortholog and only ... (Britz and Co., Wheatland, WY) in an American Association for Accreditation of Laboratory Animal Care-accredited laboratory animal facility at the University of Wisconsin School of Medicine Page of ... interpretation of the data and revision of the manuscript JEG co-conceived the study and participated in the interpretation of the data and revision of the manuscript RLS co-conceived the study and participated...
... similarities and differences in CXCR2 and its ligands between rats and humans [50] Humans express IL-8 and two IL-8 receptors, CXCR1 and CXCR2, whereas rats not express an IL-8 ortholog and only ... (Britz and Co., Wheatland, WY) in an American Association for Accreditation of Laboratory Animal Care-accredited laboratory animal facility at the University of Wisconsin School of Medicine Page of ... interpretation of the data and revision of the manuscript JEG co-conceived the study and participated in the interpretation of the data and revision of the manuscript RLS co-conceived the study and participated...
... using an analysis of variance and for post hoc comparisons of means a Newman-Keuls test was used A p < 0.05 was accepted as significant All values are expressed as the mean + one standard error of ... amelioration of markers of airway injury is likely mediated by anti-oxidant properties of DMTU Glutathione is an important endogenous antioxidant and changes in its intracellular and extracellular ... and CW contributed to the revision of the paper and provided the analysis of 4-HNE KG performed the measurements of NOx and approved the manuscript HKQ provided critical review of the paper and...
... equation and parameters compared using the extra sum of square principle [27] Parameter errors are expressed as percentage coefficient of variation (%CV) and calculated by simultaneous analysis of ... ± mg The mean resting force and the mean normalizedresponse to carbachol were 0.83 ± 0.05 g and 14 ± gr/gr of tissue, without significant differences between sensitized, challenged, and treated ... desensitization of β2-AR in both HASMC and isolated human bronchi through an acute mechanism involving PKC but not PKA, and that this desensitization might be prevented by the CysLT1R antagonist montelukast...
... (Harlan Nossan, Italy) The animals were housed in a controlled environment and provided with standard rodent chow and water Animal care was in compliance with Italian regulations on protection of ... avidin and biotin The sections were then incubated overnight with primary anti-ICAM-1 antibody (1:500), with 1:1000 dilution of primary antinitrotyrosine antibody (DBA), and anti-PAR antibody ... Salzman AL, Szabó C: Anti-inflammatory effects of mercaptoethylguanidine, a combined inhibitor of nitric oxide synthase and peroxynitrite scavenger, in carrageenan -induced models of inflammation Free...
... from 28 animals The aim of the study was to compare physiological and biochemical events to try to elucidate the mechanisms of response and nonresponse to INO in an endotoxin -induced animal lung ... this in vivo model Severity of pulmonary damage The severity of pulmonary dysfunction in terms of gas exchange and respiratory mechanics (compliance) did not differ between responders and nonresponders ... The animals were then given INO for a period of 10 minutes A single exposure to INO was given to 20 animals at hours and to another eight animals at hours after onset of lung injury Nine out of...
... GABAergic mechanism of action for propofol, but not for isoflurane Br J Anaesth 2001, 86:618-626 31 Trapani G, Altomare C, Liso G, Sanna E, Biggio G: Propofol in anesthesia Mechanism of action, ... in models of transient [4044] but not permanent [45] focal ischaemia The effects of propofol in mechanical TBI have rarely been investigated to date, although there is evidence that propofol can ... statistical analysis and drafted the manuscript RR participated in the study design and coordination and helped to draft the manuscript JW, MF and SR helped to draft the manuscript MC conceived of the...
... performance of experimental work, preliminary data analysis, and drafting of the manuscript LCF contributed to animal preparation, performance of experimental work, analysis of mechanical data, and ... performance of experimental work, analysis of mechanical and morphometrical data, and drafting of the manuscript DSO contributed to analysis of molecular biology data, and drafting of the manuscript ... and drafting of the manuscript CSNBG contributed to analysis of histological data, and drafting of the manuscript MMM contributed to analysis of molecular biology data, and drafting of the manuscript...
... different numbers of longitudinal gj-channels (panel B) Panel A of Figure shows that the presence of many (or few) transverse channels had no effect on TPT (and hence on transverse propagation ... G10 and H10, H1 and I1, I10 and J10 Assuming that each gj-channel has a conductance of 100 pS, these channels corresponded to a shunt resistance across each junction (Rgj) of 100,000 MΩ (0 channels), ... a function of the number of transverse (A) and longitudinal (B) gj-channels Panel A shows that increasing the number of transverse gj-channels decreases the TPT only when there are many longitu-...
... such as changes in smooth muscle, collagen deposition and mucus glands, is considered as another possible mechanism of AHR, especially the persistent ones (Cockcroft and Davis, 2006; Homer and Elias, ... receptor (Bisset and SchmidGrendelmeier, 2005) Enhanced expression of eotaxin-1 and CCR3 were detected in both human atopic asthmatics and animal asthma 80 models, with increased AHR and eosinophilia ... of IL-4 and IL-13 Comparing OVA group and OVA-RV group, RV infection significantly increased the expression of IL-4 (day 3) and IL-13 (day and 7) RV infection also influenced the expression of...
... exposure to nitrogen dioxide (NO2) and the severity of virus -induced asthma in children Lancet 361, 1939–1944 Chen, S., Huang, F., Tan, G., Wang, C., Huang, Y., Wang, H., et al (2009) RNA interference ... levels and asthma severity among African American, Mexican, and Puerto Rican patients with asthma J Allergy Clin Immunol 120, 137–143 Newcomb, D.C., Sajjan, U.S., Nagarkar, D.R., Wang, Q., Nanua, ... Effect of IL-5 and Eotaxin Expression in the Lung on Eosinophil Trafficking and Degranulation and the Induction of Bronchial Hyperreactivity J Immunol 164, 2142– 2150 Mukherjee, A.B., and Zhang,...