... to the activity ofthe wild-type AMPK complexes measured in the absence ofAMP (the activity ofthe wild-type complex without AMP is set to 1) (C) The activity ofthe wild-type and mutated AMPK ... Table Effect ofthe mutations in the AMPK c3 gene on theAMP dependence ofthe enzyme Fold stimulation reflects the activation ofthe corresponding AMPK complexes by 200 lM AMP relative to the basal ... been shown to bind the allosteric activator ofthe kinase, AMP, whereas the two pairs of CBS domains both bind one molecule ofAMP [20] In terms ofthe structure of AMPK, the b-subunit has been...