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23/03/2014, 12:20
... primer, 5¢-CGATCCAAGCTTTAAGGAGG AAtagGAAATGGAATTCATCGAAAAAATCCG-3¢ antisense primer, 5¢-TGCATCCATCTAGAGCATTCA GC-3¢ The amplified PCR product was digested with HindIII and XbaI, separated by agarose ... sonicate was centrifuged at 15 000 g for 20 at °C After centrifugation, the resulting supernatant was fractionated with solid ammonium sulfate The precipitate obtained at 50–70% saturation was ... Xanthomonas pathogens with differing host specificities Nature 417, 459–463 Kaneko, T., Nakamura, Y., Sato, S., Asamizu, E., Kato, T., Sasamoto, S., Watanabe, A. , Idesawa, K., Ishikawa, A. , Kawashima,...