amino acids to hydroxymethyl groups of supports
Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx
... mutagenesis of Ntcp (Eur J Biochem 270) 1121 Fig Topology model of rat Ntcp based on hydropathy analysis of the amino acid sequence (according to [1]) Transmembrane domains are symbolized as blocks of amino ... expression of the injected cRNA caused this lack of transport Therefore, up to three times the amount of the cRNA compared to the standard amount was injected into oocytes, i.e 6.9 ng instead of 2.3 ... the Baylor College of Medicine Search Launcher (Houston, USA) to identify conserved negatively charged amino acids and cysteines Tagging of Ntcp mutants by the FLAGÒ motif To determine whether...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
... grouped together as follows: aromatic amino acids (Phe, Tyr, and Trp); negatively charged amino acids (Asp and Glu); positively charged amino acids (Lys and Arg); small aliphatic amino acids (Gly ... used to determine the amount of phage detached in each round of selection Dilutions of the supernatant were plated in 2.5 mL of 0.6% top agarose containing 300 lL of E coli (BLT5615), 100 lL of ... aliphatic amino acids (Val, Leu, Ile, and Pro); and hydrophilic amino acids (Ser, Thr, His, Asn, Gln, Cys, and Met) The nomenclature of Schechter and Berger [5] was adopted to designate the amino acids...
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Biodegradable Polymers Composed of Naturally Occurring α-Amino Acids
... products This leads to imbalance of stoichiometry and contributes to the limitation of chain growth In spite of this, Huang’s study initiated a rational synthesis of a large variety of key monomers ... refluxed benzene or toluene in the presence of TosOH monohydrate (2 mol), Scheme 5.1 The presence of TosOH•H2O (2 mol) serves as both the reaction catalyst and amino group protector, preventing undesirable ... cause of low-molecular-weight polymers presumably is a high tendency of alkyl esters of α-AAs to enter into various undesirable self-condensation reactions [62] with the formation of diketopiperazines...
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Microbial Production of Amino Acids in Japan
... soybea1ns contain lower amounts of lysine, which is one of Microbial Production of Amino Acids in Japan 77 the essential amino acids for those livestocks The estimated amount of l-lysine produced in ... successful introduction of the technology, various methods were searched for and developed for microbial production of other amino acids Today a whole array of amino acids are produced by microbial ... various amounts of sodium pyruvate, g of ammonium acetate, 0.6 g of pyrocatechol, 0.2 g of sodium sulfite, 0.1 g of EDTA, and cells harvested from 100 ml of broth, in a total volume of 100 ml.The...
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Tài liệu Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase doc
... chain of any bound amino acid The lability of the a-proton observed for a large number of amino acids [5] under the action of TPL gives evidence for the orthogonal orientation of the a-proton with ... with respect to the cofactor plane [17], and shows that the pattern of binding is the same for a variety of amino acids It has been established [5] that for a number of amino acid inhibitors bearing ... reasonable to conclude that the transfer of the proton (or deuteron) from the amino group to the a-carbon atom of the quinonoid intermediate should go faster than the isotopic exchange of the proton...
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Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx
... sequence of the open reading frame (ORF) of their cDNAs and the corresponding aa sequences, in Table Summary of the purification of DNases I from the pancreases of three species of snake The results of ... from reptiles to birds after deletion of Ser205 from the enzymes of amphibians as they evolved into reptiles Thermal stability of the enzyme might be evaluated as one of the factors that reflect ... presence of Val67 is one of the essential factors responsible for the actin-binding capacity of these snake DNases I Tissue distribution of snake DNases I Fig Electrophoretic patterns of snake...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
... reaction was stopped by transferring the tubes to an ice bath The amount of fructose produced was determined by the cysteine/carbazole/sulfuric acid method [33] To determine the effect of temperature ... One unit of isomerase activity is defined as the amount of enzyme that produces mmol of product per under the assay conditions Activation of apo-BLXI by metals To examine the effect of bivalent ... eds), pp 339–357 Stockton Press, New York Converti, A & Del Borghi, M (1998) Kinetics of glucose isomerization to fructose by immobilized glucose isomerase in the presence of substrate protection...
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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
... activities of NBD2A and NBD2B could result from an inhibitory effect of the C-terminal 42 amino acids of NBD2B or a stimulatory effect of the equivalent amino acids of NBD2A To determine which of these ... last 42 amino acids Figure 2A and Table show that the ATPase activity of NBD2-DC was greater than that of SUR2B but similar to that of NBD2A, favoring the idea that the last 42 amino acids of NBD2B ... of the last 42 amino acids of SUR2 One possibility is that interaction of the heterodimer produces a conformational change that physically reduces the inhibitory effect of the last 42 amino acids...
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Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx
... Transcription factors involved in the action of amino acids on gene expression Amino acid(s) manipulations Factor(s) implied Inhibiting effect resulting from the presence of amino acids Pooled amino acids ... modifications or modulation of their activators or inhibitors The amino acid appears to be a valuable tool to study the potential diversity of AAR pathways, but despite its central regulatory role in numerous ... glutamine), together with the identified transcription factors and the responsive elements involved Most of the data concern the inhibitory effect of amino acids Initial studies were performed to explore...
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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx
... glycogen synthase, phosphofructokinase 2, ceramide synthesis, glucose uptake, apoptosis, insulin receptor substrate 1, mammalian target of rapamycin kinase (mTOR), mitogen-activated protein kinase ... feature of the activation of AMPK by fatty acids and one that is common to the ÔclassicalÕ activation pathway However at this time we cannot discount the possibility that exposure to fatty acids ... sensitivity to AMP after exposure of hearts to fatty acid (Table 1) The AMP binding site on AMPK appears to be a higher order structure contributed by two or more of the a, b and c subunits of the...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... combination of ionexchange chromatography, Lentil-Lectin Sepharose chromatography and chromatofocusing, as described previously [21] Results Expression and secretion of truncated variants of GCPII To ... seems to be essential for its hydrolytic activity [15] The protein is proposed to consist of six domains: the N-terminal cytoplasmic tail (amino acids 1–18), the helical transmembrane region (amino ... Recently, a 3D model of the extracellular region of rat GCPII has been published [20] In addition to the model of the ligand-free protein, the authors docked several GCPII inhibitors into the GCPII-binding...
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Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt
... the introduction of two adjacent b -amino acids induced a 10-membered hydrogen-bonded turn (C10) analogous to the b-turn made of a -amino acids [20] This propensity of b -amino acids to mimic canonical ... structures of b -amino acids overlap the canonical structures of a -amino acids? Subsequently, is it possible to substitute one a -amino acid in the sequence of a linear peptide for one b -amino acid ... overlap any conformation of a -amino acids In order to visualize on the potential energy surfaces the conformers of b -amino acids that fit with canonical conformations of a -amino acids, two (/ ) w) Ramachandran...
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Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt
... Nmyristoylation In the present study, to examine the effect of the combination of amino acids at positions and on protein N-myristoylation, sequential vertical-scanning mutagenesis of the amino acids ... the combination of amino acids at positions and might be a critical determinant for protein N-myristoylation In this study, to examine the effect of the combination of amino acids at positions ... addition to the combination of amino acids at positions and 6, it was demonstrated that the combination of amino acids at positions and also affects the susceptibility of the protein to protein...
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Báo cáo khoa học: Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx
... half-lives of plasmin in these experiments (also before 50% of the plasmin activity was inhibited) Computer model of the C-terminal 40 amino acids in antiplasmin A computer model of the C-terminal 40 amino ... established Mutagenesis of antiplasmin The cDNA of wt-antiplasmin, subcloned into the expression vector pMT/Bip/V5 at the BglII and XhoI sites, was used to produce the selected mutants of antiplasmin ... from one hydrophobic to another hydrophobic amino acid distant from the C-terminal portion of antiplasmin seems therefore to be of little importance Expression and purification of antiplasmin variants...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... substitutions of amino acids flanking 23FQNLF27 reduced the binding to AR LBD Most prominent inhibitory effects were found for amino acid residues directly flanking 23FQNLF27 Note that expression levels of ... fragment from pAR34 [23] into the corresponding sites of pGEX-2TK-CHB yielded pGSTAR.LBD Insertion of the AR LBD BamHI/SalI fragment of pGST-AR.LBD into the corresponding sites of pCMVGST [25] yielded ... without amino acids, 2% w/v glucose, pH 5.8), supplemented with the required amino acids Yeast transformation was carried out according to the lithium acetate method [26] A yeast liquid b-galactosidase...
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Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc
... modifications of amino acids Chemical modifications of arginine, lysine, carboxyl groups, tryptophan and histidine were performed as described before [28] The number of essential residues of a certain amino ... stability of the proteins [28] The C terminus of CpcF shows only little homology for as much as 50 amino acids A truncation by 53 amino acids reduced the solubility of the protein, possibly due to misfolding, ... observation of low activities of chromophore binding to all binding sites studied, including a-C84 of PecA [16–18], by the site-selective effect of Triton X-100 (TX100) on chromophore binding to PecB,...
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Báo cáo khoa học: Inhibition of a-synuclein fibrillization by dopamine analogs via reaction with the amino groups of a-synuclein Implication for dopaminergic neurodegeneration pot
... region of HSQC spectra reflect formation of imino groups during reaction of the side-chain amino groups with Q or DAQ Fig Cytotoxicity of DA-modified a-Syn (A) Time course of the MTT reduction of different ... compared with that of intact a-Syn This suggests that at least three amino groups of an a-Syn molecule are modified by Q or DA to generate imino groups that link to the aromatic ring of quinone and ... concentration of a-Syn was 200 lM and that of the compounds was mM (B) Time courses of the reactions of Q with a-Syn (circle) and some amino acids as recorded at 345 nm The amino acids are Gly...
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Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt
... transport of amino acids under photorespiratory and nonphotorespiratory conditions Results Expression of the genes for GOGATs and CPSase In order to understand the physiological role of GOGATs ... that the mitochondria are highly capable of using glycine to convert ornithine to citrulline (up to 126 lmolặh)1ặmg)1 protein) [9] Because of a lack of bioinformatic tools to predict to what extent ... ammonium 48 h after transfer from high CO2 to air, owing to photorespiratory ammonium release (Fig 3A) A slight accumulation of photorespiratory and nonphotorespiratory ammonium was detected in the GLT...
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Báo cáo khoa học: D-Amino acids in the brain: the biochemistry of brain serine racemase potx
... assembly of the receptor and accessory proteins GRIP can bind to the glutamate receptor subunit (GluR2) subunit of AMPA receptors via PDZ4 ⁄ PDZ5, both PDZ domains working in concert to establish ... simultaneous phosphorylation of the GluR2 subunit of the AMPAR together with the phosphorylation of serine racemase releases GRIP to the cytoplasm in the absence of any dual interaction A L-Ser ... carboxy-terminal end of the polypeptide chain, disrupts its interaction with PDZ4 ⁄ PDZ5 of GRIP, it is tempting to speculate that phosphorylation of Ser336 of human serine racemase or of Thr336 of mouse...
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