alterations in protein fractions

Báo cáo khoa học: Concepts and tools to exploit the potential of bacterial inclusion bodies in protein science and biotechnology pdf

Ngày tải lên : 06/03/2014, 00:20

... bottlenecks in the folding of speciﬁc proteins, for example those containing disulﬁde bonds or requiring cofactors, multidomain proteins, fusion proteins In the following we summarize recent progress in ... of protein deposition and removal, and further suggested that aggregated proteins can complete their folding and activation process once deposited in IBs [42] Protein protein interactions within ... result in crosslinking and misfolding Recombinant protein production might induce aggregation and elicit stress responses Heterologous protein production is by itself cause of toxicity for cells, independently...

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Báo cáo khoa học: Suppression of a cold-sensitive mutant initiation factor 1 by alterations in the 23S rRNA maturation region pdf

Ngày tải lên : 06/03/2014, 00:21

... to introduce mutations into pKK3535 A deletion was introduced into the gene rnc, encoding the RNase III, with pKO3::Drnc [36] As a result, only 15 N-terminal and 38 C-terminal amino acids remained ... R69L mutant of IF1 allows premature subunit joining by either failing to discriminate between certain TIR elements in mRNA, or inducing a conformation in the 30S subunit under nonpermissive cold ... coli contains an oligomer-binding motif EMBO J 16, 1436–1443 Weber MH, Beckering CL & Marahiel MA (2001) Complementation of cold shock proteins by translation initiation factor IF1 in vivo J...

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Báo cáo khoa học: Many fructosamine 3-kinase homologues in bacteria are ribulosamine⁄erythrulosamine 3-kinases potentially involved in protein deglycation docx

Ngày tải lên : 07/03/2014, 05:20

... Hypothetical binding proteins protein Cytophaga hutchinsonii Outer Membrane Protein PFKb Hypothetical protein FN3K YniB YniC Hydrolase Escherichia coli Nocardioides sp., Rubrobacter xylanophilus, Salinibacter ... Matthijs G & Van Schaftingen E (2003) A mammalian protein homologous to fructosamine-3-kinase is a ketosamine-3kinase acting on psicosamines and ribulosamines but not on fructosamines Diabetes 12, ... compounds, including ribuloselysine and erythruloselysine It was also inactive on more than 50 other potential phosphate acceptors, including d-ribulose, d-xylulose, choline, ethanolamine, l-serine,...

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Báo cáo khoa học: Flexible nets The roles of intrinsic disorder in protein interaction networks potx

Ngày tải lên : 07/03/2014, 21:20

... regions of the protein involved in interaction with Cdk2 and cyclin A: 1, domain interacting with cyclin A; 2, a linker helix involved in binding both cyclin A and Cdk2; 3, domain interacting with ... biological protein protein interaction networks maintain a scale-free nature throughout full interactomes remains to be verified Secondly, experimental protein protein interaction data contains a significant ... alpha [193]; BRCA1, cyclin A [194] profilin [196]; deoxyribonuclease I, vitamin D binding protein, thymosin beta-4, cofilin [197] neurogranin, calcineurin, AC1 [198]; calponin [199]; caldesmon [200]...

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$Báo cáo khoa học: Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C pdf$

Báo cáo khoa học: Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C pdf

Ngày tải lên : 08/03/2014, 08:20

... enterotoxin in human intestine and in a human intestinal cell line (Caco-2) J Cell Physiol 156, 138–144 20 Cohen, M.B., Hawkins, J.A & Witte, D.P (1998) Guanylin mRNA expression in human intestine ... Disc-large, ZO-1 (PDZ) domain protein which interacts with GC-C was identiﬁed in a yeast two hybrid screen [44] This protein named Ôintestine and kidney-enriched PDZ protein (IKEPP) interacts with GC-C ... soybean trypsin inhibitor, lgÆmL)1 leupeptin, lgÆmL)1 aprotinin and 100 lM sodium orthovanadate The protein was estimated by using a modiﬁcation of the Bradford protein assay [29] In vitro guanylyl...

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Báo cáo khoa học: CHIP participates in protein triage decisions by preferentially ubiquitinating Hsp70-bound substrates pdf

Ngày tải lên : 15/03/2014, 23:20

... glycoproteins are folded in the calnexin ⁄ calreticulin cycle [48] Misfolded glycoproteins are bound in turn by the chaperones calnexin or calreticulin and the folding sensor UDP-glucoseglycoprotein-glucosyltransferase ... conditions [14,33] In the absence of a J-domain co-chaperone, c-phosphate cleavage is rate-limiting in the ATPase cycle of Hsp70 proteins [34,35] In the presence of a J-domain protein, nucleotide ... polyubiquitination Ubiquitination of a native chaperone substrate protein Hsp70 and Hsp90 not only interact with misfolded proteins but also with native or near-native proteins To investigate...

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Báo cáo khoa học: Signal peptide hydrophobicity is critical for early stages in protein export by Bacillus subtilis ppt

Ngày tải lên : 16/03/2014, 22:20

... ribosome-nascent chain complexes (RNCs) A C-terminal 4· methionine tag was introduced into the nascent chains to increase the labelling efﬁciency The nascent chain corresponding to the authentic preprotein comprised ... SRP cross-linking [18] In addition, hydrophobic alterations in the signal peptides of SecB-dependent proteins, re-routed these proteins into the SRP pathway [19,24,25] Precursor proteins from Gram-positive ... nascent chain; ?, unknown cross-linking adducts; *, cross-linking adducts with E coli L23; d, cross-linking adducts with E coli Ffh; s, cross-linking adducts with B subtilis Ffh; n, cross-linking adducts...

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Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx

Ngày tải lên : 17/03/2014, 09:20

... sterical hindrance between protein S and MoAb 44 Discussion The binding site for protein S on C4BP is predominantly contained in CCP1 of the b-chain [9] A key binding surface for protein S involves ... b-chain in immobilized C4BP In uence of protein S binding of monoclonal antibodies To investigate whether protein S and MoAb 15/ MoAb 44 had overlapping binding sites on the b chain, the following ... bind 125 I-labeled protein S in solution (Fig 2C) All variants yielded binding patterns similar to that of wild-type b-chain In the binding assay, the recombinant proteins were immobilized in...

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Báo cáo khoa học: Alterations in the photoactivation pathway of rhodopsin mutants associated with retinitis pigmentosa potx

Ngày tải lên : 22/03/2014, 16:20

... resulting in higher A280 ⁄ A500 ratios, which may not necessarily reﬂect misfolding of the protein Furthermore, many studies claiming that mutations in rhodopsin cause RP mainly by protein misfolding ... tolerated both in the inactive and in the G -protein binding states In view of all this experimental evidence, we propose that the mutants in the present study follow an activation pathway that includes ... (1997) G -protein- coupled receptors: molecular mechanisms involved in receptor activation and selectivity of G -protein recognition FASEB J 11, 346–354 Marinissen MJ & Gutkind JS (2001) G -protein- coupled...

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Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot

Ngày tải lên : 22/03/2014, 16:21

... intrinsic relationship between the RNA binding and protein folding in RNAUDE Interestingly, treatment of RNAUDE with RNase A did not result in signicant changes of the melting curve, indicating ... polypeptide chain, helping secondary structure formation Moreover, direct interaction of rRNA with proteins, especially with nucleic acid-binding proteins, also has an effect on protein refolding in vitro ... cotranslational folding Ligand binding often stabilizes the protein structures by enhancing the folding of exible or disordered regions, especially in the case of nucleic acid-binding proteins (e.g bateriophage...

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A Single-Molecule Perspective on the Role of Solvent Hydrogen Bonds in Protein Folding and Chemical Reactions pptx

Ngày tải lên : 22/03/2014, 18:20

... combined with polyprotein engineering has become a powerful approach to studying proteins Using this technique, we have investigated the force-dependency of protein folding,[46, 47] unfolding[37, ... extend single (I27SÀS)8 polyproteins The constant force caused individual I27 proteins in the chain to unfold, resulting in stepwise increases in length of the molecule following each unfolding event ... stretching the protein again at ~ 180 pN, two of the four modules unfold again, bringing the polyprotein to its original unfolded length Subsequently a further two modules in the polyprotein unfold...

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báo cáo hóa học:" Alterations in vitamin D status and anti-microbial peptide levels in patients in the intensive care unit with sepsis" pot

Ngày tải lên : 18/06/2014, 15:20

... levels of vitamin D bind- http://www.translational-medicine.com/content/7/1/28 ing protein (DBP), the major carrier protein of vitamin D, are decreased in the setting of sepsis leading to lowered ... Figure jects sepsis, vitamin ill subjects without critically subjects with Plasma criticallyD binding protein insepsis andillhealthy subPlasma vitamin D binding protein in critically ill subjects ... circulating forms of vitamin D, 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D, but it is also a scavenger of monomeric actin thus preventing its polymerization into F-actin [25,26] The actin binding...

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Báo cáo hóa học: " Alterations in intracellular potassium concentration by HIV-1 and SIV Nef" pptx

Ngày tải lên : 20/06/2014, 01:20

... bated with in intracellular K+ and SIV Nef protein Alterations recombinant HIV- 1in T-lymphoblastoid cells incuAlterations in intracellular K+ in T-lymphoblastoid cells incubated with recombinant HIV-1 ... cytotoxic against both CD4+ T cells and monocytoid cell lines [25] Several viral proteins, including influenza A virus M2, influenza B virus NB, HIV-1 Env, Vpu, and Vpr, induce alterations in cellular ... HIV-infected cells in patients Antibodies against Nef have been detected in HIV-infected individuals [33], suggesting the extracellular presence of Nef in vivo The concentrations of Nef used in...

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ADVANCES IN PROTEIN KINASES ppt

Ngày tải lên : 28/06/2014, 14:20

... remaining proteins were divided into the following groups; WD-40-repeat proteins with a SOCS box (WSB proteins), ankyrin repeat proteins with a SOCS box (ASB proteins), sprouty (SPRY) domain-containing ... coiled-coil domain, a DNA binding domain, a linker domain, an SH2 domain and a C-terminal transactivation domain The N-terminal domain is involved in Advances in Protein Kinases STAT dimerisation and ... box proteins (SSB proteins) and GTPase domain-containing proteins (RAR and RARlike proteins) (Krebs & Hilton, 2001) In addition to a central SH2 domain, all members of the SOCS family contain...

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Báo cáo sinh học: "Dimerization in protein kinase signaling" ppsx

Ngày tải lên : 06/08/2014, 18:21

... kinase TEC - Tec protein- tyrosine kinase ZAP70 - 70 kDa zeta T chain-associated kinase Non-receptor protein- serine/threonine kinases • Doubled catalytic domain kinases GCN2 - Kinase related to ... kinase BMX - Bone marrow kinase in chromosome X BTK - Bruton’s tyrosine kinase FAK - Focal adhesion kinase ITK - T cell specific kinase Pyk2 - Protein- tyrosine kinase Syk - Spleen tyrosine kinase ... lineage kinases DLK - Dual leucine zipper bearing kinase LZK - Leucine zipper bearing kinase MLK3 - Mixed lineage kinase In conclusion, dimerization has a crucial role in the regulation of many kinases,...

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Báo cáo y học: "Alterations in Arterial Blood Parameters in Patients with Liver Cirrhosis and Ascite" docx

Ngày tải lên : 08/08/2014, 18:20

... Conclusions In conclusion, in patients suffering from severe liver cirrhosis and ascites, portopulmonary shunt, intrapulmonary shunt and VA/Q inequality may induce a decrease in PaO2 and SaO2 in association ... is a common finding in patients with cirrhosis particularly in those with Child-Pugh grade C, (grade < 14), in whom, furthermore, clinical manifestations were rare [20-23] According to previously ... Fluid in the chest (pleurisity) may be found in at least 10 percent of patients with cirrhosis, being more common on the right side influencing when in large quantities the VA/Q ratio In the...

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Báo cáo y học: "Alterations in the complement cascade in post-traumatic stress disorder" potx

Ngày tải lên : 08/08/2014, 21:20

... receptor and regulator proteins It is a complex cascade involving proteolytic cleavage of serum glycoproteins often activated by cell receptors This cascade ultimately results in induction of the antibody ... neuroendocrine mechanisms related to PTSD [40,41] modulating the immune function [42,43] might affect the initial steps in the inflammatory cascade and thus influence alterations in the functional ... immune function in health and disease: neural, endocrine and immune interactions Brain Res Rev 1997, 23:79-133 43 Rohleder N, Karl A: Role of endocrine and inflammatory alterations in comorbid somatic...

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$Báo cáo khoa học: "Micronutrients in biomass fractions of holm oak, beech and fir forests of the Montseny massif (Catalonia, NE Spain)" pot$

Báo cáo khoa học: "Micronutrients in biomass fractions of holm oak, beech and fir forests of the Montseny massif (Catalonia, NE Spain)" pot

Ngày tải lên : 09/08/2014, 03:24

... analysed in order to determine individual tree variability in the different biomass fractions of 11 holm oaks The variability of the different elements is less than 12% in all the biomass fractions ... seen in table I In the holm oak, we can combine our data with those of Ferres (1984) for major nutrients to obtain the following nutrient ranking: Ca > N > K > Mg P > Mn > Fe > Na > Zn > Cu In ... Pressler borer In the beech stand (Santa Fe) we collected samples of leaves, twigs and thin we The concentrations in the different fractions agree with the general trends discussed in the introduction,...

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Báo cáo y học: "Alterations in peripheral blood memory B cells in patients with active rheumatoid arthritis are dependent on the action of tumour necrosis factor" potx

Ngày tải lên : 09/08/2014, 14:21

... lymphocytes into the inflamed synovium In order to study the changes in peripheral memory B cell subpopulations in RA patients, and to understand the possible role of TNF in regulating changes in specific ... Isolated PBMCs were stained by standard methods with fluorescein isothiocyanate (FITC), phycoerythrin (PE), peridinin-chlorophyll -protein Cy5.5 (PerCpCy5.5) or allophycocyanin (APC) conjugated mAb ... showing the difference in CD27 expression in peripheral blood and synovial CD19+ B cells Dashed line shows staining with the isotype control and the solid line for CD27 (b) Box-plots representing...

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Báo cáo y học: " Alterations in hippocampal serotonergic and INSR function in streptozotocin induced diabetic rats exposed to stress: neuroprotective role of pyridoxine and Aegle marmelose" doc

Ngày tải lên : 10/08/2014, 05:21

... from bound insulin intercede by increase in the insulin receptor Treatment with pyridoxine alone and in combination with insulin and Aegle marmelose to diabetic rats caused a reversal in the B ... Diabetic+Insulin Diabetic+Pyridoxine Diabetic+Insulin+Pyridoxine Bound/free (fmoles/mg protein) 80 60 40 20 0 50 100 150 200 250 Bound (fmoles/mg protein) b Bound/free (fmoles/mg protein) 100 ... protein/ nM) 400 Control Diabetic Diabetic+Insulin Diabetic+Pyridoxine Diabetic+Insulin+Pyridoxine 300 200 100 0 50 100 150 200 250 300 Bound (fmoles/mg protein) b Bound/free (fmoles/mg protein/ nM)...

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