alpha mmp 9 and cyclind1 protein expression in mda mb 231 and mcf 7 cells by western blot analysis
báo cáo khoa học: "Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells" pptx
... cell lines MDA- MB- 231, MDA- MB- 435S, MDAMB-453, SK-Br-3, T47D, ZR -75 -1 and normal breast cells HBL-60; the gene was highly expressed in MDA- MB4 35S, T47D, MCF- 7 We also detected IDO expression in ... (B) IDO gene integration and transcription by PCR and RT-PCR (C) Western blot analysis of IDO protein expression in CHO-IDO cells using anti-IDO antibody In transfected group, CHO cells transfected ... kDa IDO protein, indicating that CHO cells stably transfected with IDO could produce IDO protein (D) Analysis of free amino acids in culture supernatant Amino acid level in CHO cells 72 h after...
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Pulmonary tuberculosis associated with increased number and percentage of natural killer and B cells in the peripheral blood pot
... 1354.4 ± 244.4 77 5 .7 ± 225.4 33.1 ± 7. 5 177 .1 ± 133 .7 248.8 ± 96 .4 205 .7 + 69 P value 0.0 670 0.0250* 0 .7 190 0.0000* 0 .96 90 0.0250* PTB = Pulmonary tuberculosis (n = 10), normal healthy Individuals ... response against MTB by increasing the infection inoculum (Vordermeier et al., 199 6; Maglione et al., 20 07) In addition the protection of mice against MTB infection by administration of intravenous ... al., 198 5; Onwubalili et al., 198 7; Singhal et al., 198 9; Jones et al., 199 7) In this study decreased CD4 + counts and percentage were observed in patients with PTB, whereas the CD8 + cells were...
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Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
... IL- 17, IL-10, and TGFβ in the culture supernatant were measured by sandwich ELISA Flow cytometric analysis of intracellular indoleamine 2,3-dioxygenase and regulatory T cells Single mononuclear cells ... monocyte-derived macrophages, and in vitro-derived DCs induce IDO expression and inhibit T-cell proliferation In this regard, it will be interesting to investigate IDO expression in different subsets ... 44: 191 7- 192 7 Garcia G, Komagata Y, Slavin AJ, Maron R, Weiner HL: Suppression of collagen-induced arthritis by oral or nasal administration of type II collagen J Autoimmun 199 9, 13:315-324 Weiner...
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Báo cáo khoa học: Effect of 5-lipoxygenase inhibitor MK591 on early molecular and signaling events induced by staphylococcal enterotoxin B in human peripheral blood mononuclear cells doc
... determined JNK SEB SB-203580 MK 591 MK886 Curcumin SP600125 PD980 59 500 178 .4 212.5 173 .5 168 74 .2 3 37 p38kinase ± ± ± ± ± ± ± 29. 5 18.6 78 .5 19. 5 22 2.5 25 MAPK 250 34 76 55 43 3 37 225 2 79 38.5 71 .5 ... lipoxygenase inhibitors (MK886, MK 591 and curcumin), p38kinase inhibitor (SB203580), JNK inhibitor (SP600125) and MAPK inhibitor (PD980 59) to observe the effect in human PBMCs As all investigated protein ... penicillin G sodium, 20 lgÆmL)1 streptomycin sulfate, 20 lgÆmL)1 kanamycin sulfate, and minimal essential Immunoblotting and western blotting The cells were lysed after a brief sonication and incubated...
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Báo cáo y học: "Nature of Regulatory T Cells in the Context of Allergic Disease." ppsx
... and asthma J Allergy Clin Immunol 2001;108: 92 1–8 27 Durham SR, Varney MA, Gaga M, et al Grass pollen immunotherapy decreases the number of mast cells in the skin Clin Exp Allergy 199 9; 29: 1 490 –6 ... immunotherapy inhibits allergen-induced infiltration of CD4+ T lymphocytes and eosinophils in the nasal mucosa and increases the number of cells expressing messenger RNA for interferon-c J Allergy Clin ... tolerance induced by SIT includes suppression of T cells and switching of antibody profile into noninflammatory types IgG4 and IgA, with a decrease in IgE Effector cells such as mast cells, eosinophils,...
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Báo cáo y học: "Decreased effector memory CD45RA+CD62L– CD8+ T cells and increased central memory CD45RA–CD62L+ CD8+ T cells in peripheral blood of rheumatoid arthritis patients" ppt
... indicating increased recruitment into the inflamed synovium in RA Inflammation and production of chemokines such as macrophage inflammatory protein- 1α and RANTES [7, 8] in the synovium may result in ... set up in RA in which there is continuous generation of CD8+ T cells that can be recruited into the synovium, resulting in chronic inflammation and joint destruction Recently, memory CD8+ T cells ... CD 29 bright+) peripheral blood T-lymphocytes in health and in rheumatoid arthritis Rheumatol Int 199 6, 16 :77 87 Mueller YM, De Rosa SC, Hutton JA, Witek J, Roederer M, Altman JD, Katsikis PD: Increased...
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Báo cáo y học: "Direct Toll-like receptor 2 mediated co-stimulation of T cells in the mouse system as a basis for chronic inflammatory joint disease" ppsx
... CD40/CD154 interactions in the priming, differentiation, and effector func- 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 tion of helper and cytotoxic T cells J Leukoc Biol 199 8, 63:418-428 ... resting CD4+ T cells by the interaction of VLA-4 and VLA-5 with fibronectin or VLA-6 with laminin J Immunol 199 0, 145: 59- 67 22 Brett SJ, Mazurov AV, Charles IG, Tite JP: The invasin protein of ... the third restimulation in vitro, the three CTL lines consisted of more than 99 % T cells (Thy-1.2+), including 93 .1 97 .4% CD8+ and 0 .7 2.0% CD4+ T cells (Fig legend) When incubated on plate-bound...
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Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot
... Nature 199 7, 3 89: 73 7 -74 2 Groux H, Bigler M, de Vries JE, Roncarolo MG: Interleukin-10 induces a long-term antigen-specific anergic state in human CD4+ T cells J Exp Med 199 6, 184: 19- 29 Jonuleit ... inflammation, and the TR cells inhibit proliferation of antigen-specific and bystander T cells This results in a small number of CD4+ T cells surviving, which persist as memory T cells 15 16 17 18 19 of ... Roberts AB, Sporn MB, Weiner HL: Suppressor T cells generated by oral tolerization to myelin basic protein suppress both in vitro and in vivo immune responses by the release of transforming growth factor...
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The role of CD8 t cells in the differentiation of TNF iNOS producing dendritic cells and TH1 responses
... during the early phases of infection (Gazzinelli et al., 199 4) It also plays an important role by activating bystander resting T cells and maintaining the antigen specific Th1 response (Kubin ... uninfected cells in a bystander manner (Mitrovic et al., 199 4) 1 .7. 2 Bystander activation of T -cells Bystander activation of T cells, i.e the stimulation of unrelated, heterologous T -cells by ... chromosome and in humans, and chromosome and 11 in mice respectively (Trinchieri, 199 8) These two genes are regulated independently (Trinchieri, 199 8) and have to be expressed in a highly coordinated...
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Báo cáo khoa học: " An immunohistochemical study of chromogranin A and Sp-1 immunoreactive cells in the gastrointestinal tract of ovariectomized rats" pdf
... yhotiS ,M yhlaS-lE 91 4-314 ,242 , 79 9 1 deM nretnI J esaesid lewob yrotammalfni ni sllec enircodne cinoloC L suilemirG ,R gnilnetS ,A nossleinaD ,M yhlaS-lE 570 1 -96 01 ,31 , 899 1 lohtapotsiH lotsiH ... ihsietaT ,D sinuA ,A ihsokanuF ,N arumiK 41 106- 395 ,41 ,99 91 seR reniM enoB J noitprosbalam muiclac lasuaponemtsop fo ledom enirum dezimotceiravO C ,nehC ,ND ulaK 31 191 - 571 ,51 , 199 1 seR reniM ... sllec RI-GCB fo rebmuN giF 591 star citoropoetso dezimotceiravo fo TIG ni sllec RI ninargomorhC . 0 97 1-6 871 ,94 ,4002 icS siD giD aitreni cinoloc htiw stneitap fo asocum cinoloc ni sllec evitcaeronummi...
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Báo cáo y học: "Enhanced effector function of cytotoxic cells in the induced sputum of COPD patients" doc
... Lymphocytes, % 0 .9 (0.3-1.3) 3 .9 (0 .7- 7.4) 9. 5 (3.2-13.5) COPD vs Smokers p < 0.05 COPD vs HNS p < 0.001 0 .7 (0-1.2) 0.6 (0.1-0 .9) 1.3 (0.2-1 .9) NS TCC, × 1 07 cells/ g Eosinophils, × 1 07 cells/ g Eosinophils, ... × 1 07 cells/ g 0.53 (0.14-0.63) 2. 37 (1.23-4.06) 7 .94 (3.82-12.13) COPD vs Smokers p < 0.01 COPD vs HNS p < 0.001 Macrophages, × 1 07 cells/ g 0.58 (0.21-0 .95 ) 2.41 (0.88-3 .99 )) 1. 89 (0 .76 -2 .75 ) ... (44 -73 ) Gender (M/F) 1/4 5/5 6/5 Packs/yrs Smoking status (Current/Ex) Chronic bronchitis (Yes/No) (0) 35 ( 19- 51) 10/0 51 ( 27- 77) 5/6 4/6 7/ 4 112 (88-124) 78 (72 -86) 100 ( 89- 122) 74 (73 -82) 59...
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Báo cáo y học: " Aberrant splicing of the hRasGRP4 transcript and decreased levels of this signaling protein in the peripheral blood mononuclear cells in a subset of patients with rheumatoid arthritis" pdf
... solution containing 3,3'-diaminobenzidine tetrahydrochloride (DAB) Nuclear staining was done with hematoxylin, and the resulting stained cells were examined by light microscopy Immunoblotting After ... number: [FJ768 677 ]); the first 2 07 nucleotides of exon (splice variant 6, GenBank accession number: [FJ768 678 ]); exon (splice variant 7, GenBank accession number: [FJ7686 79 ] ); exons 7, 8, and ... were also determined RasGRP4 protein expression in PBMCs/monocytes was evaluated Recombinant hRasGRP4 was expressed in mammalian cells Results: Circulating CD14+ cells in normal individuals were...
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Báo cáo y học: " In situ detection of Gag-specific CD8+ cells in the GI tract of SIV infected Rhesus macaques" pptx
... Total cells/ mm2 CD8+ cells/ mm2 Gag CM9+ cells/ mm2 % Gag CM9 of CD8+ cells RM RM Spleen Spleen 16 678 (± 032) 20 7 89 (± 552) 399 (± 386) 048 (± 6 27) 164 (± 39) 198 (± 70 ) 6.80 (± 0.6) 9. 59 (± 2.0) ... Spleen 20 276 (± 877 ) 694 (± 494 ) 206 (± 49) 7. 78 (± 2.0) RM Submandibular LN 31 340 (± 076 ) 333 (± 511) 170 (± 11) 5.21 (± 1.1) RM Submandibular LN 20 7 89 (± 552) 885 (± 134) 255 (± 78 ) 5.16 (± ... verifying that nonspecific binding of the Qdot 655 multimer is low Staining pattern and staining intensity of Gag CM9 Qdot 655 multimer positive cells Confocal microscopy revealed a punctate staining...
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Báo cáo khoa học: " Water extraction by tree fine roots in the forest floor of a temperate Fagus-Quercus forest" pdf
... remarkably con- ing the summers in 199 1 and 199 2 Values -1 peaked at 0.8 and 1.0 mm d in the wet months August 199 1 and August 199 2 (figures4 and 5) Even in the dry July 199 1 a high root uptake rate ... storage during summer between 12.5 mm (for plots under beeches in 199 2, see table IV) and 32.2 mm (for plots under oaks in 199 1), root water uptake (88 and 89 mm in the summers 199 1 and 199 2, respectively) ... Bundestages, Göttingen, 199 3 Walsh R.P.D., Voight P.J., Vegetation litter: an understimated variable in hydrology and geomorphology, J Biogeo ( 1 97 7) 253- 274 2 87- 292 1 47 ( 199 2) 69- 78 Strong W.L.,...
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Báo cáo y học: " Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen" pptx
... Immunol 199 8, 160: 470 8- 471 8 Shi HN, Grusby MJ, Nagler-Anderson C: Orally induced peripheral nonresponsiveness is maintained in the absence of functional Th1 or Th2 cells J Immunol 199 9, 162:5143-5148 ... patterns of cytokine gene expression in vivo in orally tolerant mice Eur J Immunol 199 4, 24: 272 0- 272 4 Tsuji NM, Mizumachi K, Kurisaki J: Interleukin-10 secreting Peyer’s patch cells are responsible ... York: Wiley; 199 6:15.5.1-15.5. 19 Seetharaman R, Mora AL, Nabozny G, Boothby M, Chen J: Essential role of T-cell NF-kappa B activation in collageninduced arthritis J Immunol 199 9, 163:1 577 -1583 Butler...
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Báo cáo y học: "CD4+CD25+ immunoregulatory T cells may not be involved in controlling autoimmune arthritis" ppsx
... self-tolerance maintained by CD25+CD4+ naturally anergic and suppressive T cells: induction of autoimmune disease by breaking their anergic/suppressive state Int Immunol 199 8, 10: 196 9- 198 0 10 Nakamura ... Rheum 199 0, 33:866- 876 17 Mikecz K, Glant TT: Migration and homing of lymphocytes to lymphoid and synovial tissues in proteoglycan-induced murine arthritis Arthritis Rheum 199 4, 37: 1 395 -1403 ... receiving spleen cells containing CD25+ cells or spleen cells depleted of CD25+ cells were identical The number of CD4+CD25+-expressing regulatory T cells from BALB/c and C57BL/6 mice during immunization...
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Báo cáo y học: "A paragon of self-tolerance: CD25+CD4+ regulatory T cells and the control of immune responses" pps
... role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation J Exp Med 199 9, 190 : 99 5-1004 51 Berg DJ, Davidson N, Kuhn R, Muller W, Menon S, Holland G, Thompson-Snipes ... polyclonal T cell activation in vitro by inhibiting interleukin production J Exp Med 199 8, 188:2 87- 296 39 Yamazaki S, Iyoda T, Tarbell K, Olson K, Velinzon K, Inaba K, Steinman RM: Direct expansion ... they become ineffective or suppressive stimulators of TE cells (d) CD25 expression by the TR cells acts as an interleukin-2 sink and hinders the autocrine/paracrine stimulation of TE cells 23 Arthritis...
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Báo cáo y học: "The role of regulatory T cells in antigen-induced arthritis: aggravation of arthritis after depletion and amelioration after transfer of CD4+CD25+ T cells" doc
... allografts [28], in Langerhans islets and pancreatic lymph nodes in inflammation-induced diabetes [ 29] , in chronically inflamed skin in a Leishmania infection model [30], and in the mucosa and mesenteric ... destruction and pannus formation A score for inflammatory changes (Inf) was calculated by adding the points for synovial hyperplasia and infiltration, and for joint destruction (Dest) by adding the points ... antiCD4 (H1 29. 9), biotinylated anti-αE 7 (M 290 ), biotinylated anti-CD25 (7D4), allophycocyanine or FITC-conjugated anti-CD25 (PC61), streptavidin-allophycocyanine and streptavidin-PE, and matched...
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Báo cáo y học: " Expansion of CD4+CD25+ helper T cells without regulatory function in smoking and COPD" potx
... (35-65) NA NA and and FEV1/FVC % Pre bronchodilatation 77 (74 -83) 78 (75 -82) 62 (60- 67) 60 (56- 67) FEV1/FVC % Post bronchodilatation NA 78 (77 -81) 64 (62- 67) 60 ( 59- 66) Age Smoking (pack years) ... airway T cells are highly activated in COPD as indicated by increased expression of CD 69 and HLA-DR [6] In addition, CD4+ cells express high levels of CD25 in COPD and smokers, suggesting the ... COPD 19 (9. 6-22) 27 (20-42) P < 0.05 COPD ex-s vs COPD s 0.2 (0.045-0.31) 0. 49 (0.23-1.1) 0. 19 (0.06-0 .93 ) NS 0.36 (0.04-1. 07) 0. 19 (0. 07- 5.2) NS Lymphocytes 1 .7 (1.1-2.2) 2.3 (1.5-3 .9) 1.5 (0 .90 -2.8)...
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