... · 10) 8 (± 1.3) 0. 7 · 10) 1 (± 0. 07) 0. 8 · 10) 1 (± 0. 1) 1.7 · 106 (± 0. 06) 1.5 · 106 (± 0. 1) Ó FEBS 200 3 Cell -free synthesis of a disulfide-containing protein (Eur J Biochem 2 70) 4785 Fig Biotinylation ... cell -free synthesis of the active disulfide-containing scFv Materials and methods Materials The gene encoding the scFv fragment of the Se155–4 IgG antibody, in the orientation VL-linker-VH (scFvLH) ... through G25 spin column pre-equilibrated with 10 mM PBT (pH 8 .0) The biotin cuvette was first coated by streptavidin (34 ng), then by the crude protein solution including the biotinylated scFv...
... 68 103 104 – 64 43 36 90 10 57 58 18 – 12 36 90 30 28 – – 36 12 45 38 45 10 28 40 – 109 106 113 101 116 1 20 111 113 100 Interestingly, the absence of subunit also resulted in an increase in the ... strain In the case of the VZ9 strain, this was to be expected, based on the reduced growth rate of the single deletion strain lacking subunit The remaining double deletion mutants, VZ4 (D6/D7), VZ6 ... containing 1% (w /v) yeast extract, 2% (w /v) bacto-peptone, 2% (w /v) agar, 3% (v/ v) glycerol and 2% (v/ v) ethanol (YPEG) Viability of the strains on fermentable medium was confirmed on 1% (w /v) yeast...
... income (including spouse's) in 1989 is measured by a seven-category item: less than NT$3 ,00 0 (=1), NT$3 ,00 0 to NT$4,999 (=2), NT$5 ,00 0 to NT$9,999 (=3), NT$ 10, 000 to NT$14,999 (=4), NT$15 ,00 0 ... to NT$ 20, 000 (=5), NT$ 20, 000 to NT$49,999 (=6), and NT$ 50, 000 and over (=7) This income information, however, was missing for 397 cases Their income measures were replaced with the median incomes ... 1.61 2 .04 § 1 .07 3.33*** 2.82*** 1.27 14.29*** 4.97** 3.48* Having any serious conditions 13.78*** 2.81 Having any ADL 27. 70* ** 5.68*** §p
... would be produced deriving from SiNWs oxidated (Fig 4) The ring in the SAED image inserted in Fig 4(b) is from the reflection of SiO2 ( 400 ) The mechanism of SiNWs growth is explained now At high temperature, ... those of Si ( 200 ) and ( 400 ) It is well established that Si nanowires grown by the metal-catalyzed SLS technique usually have a growth direction along ( 200 ) and are single- crystalline [ 10] Therefore, ... end of nanotubes as shown by the arrow Fig 4(b) shows TEM image of an individual nanotube and the corresponding SAED pattern (diffraction ring), in which the ring is from reflection of SiO2 ( 400 )...
... 100 0 N Clà ment et al / Physica E 13 ( 200 2) 999 – 100 2 e the silicon top-layer, even for ultra-thin silicon layers (thickness as low as – 20 nm), and allows to obtain a very sharp interface ... up to 2× 105 A=cm2 ows through the wire It can be re- 100 1 Fig IDS –VDS curves and IDS –VG transconductance (inset) curves acquired at 300 K for the silicon nanowire device presented in Fig A ... for low drain–source bias voltages (0. 1– 10 mV) the drain–source current presents oscillations with respect to the gate voltage These 100 2 N Clà ment et al / Physica E 13 ( 200 2) 999 – 100 2 e Fig...
... 117 and 148 are involved in the binding to N domain of TnC [11–16] Serial deletion studies of the C terminus of TnI showed that residues 166–182 are involved in binding to the thin filament [16] ... )9 300 )94 50 )8 800 )88 50 343 343 – – 351 351 – – No 100 30 + 65c 23 ++ 52c 30 No 100 23 a In the presence of GdmCl, kMax ¼ 347 nm and mass centre ¼ 353 nm b ++, Strong binding; + weak binding [16] ... TnI(98–147) vectors were transformed in E coli strain FEBS Journal 272 ( 200 5) 779–7 90 ª 200 5 FEBS A O Tiroli et al BL21(DE3)pLysS and expressed by adding 100 mmÆL)1 of lactose at D 600 ¼ 0. 8 The induced...
... PGE1 pretreatment None Thrombin U73122 + thrombin Thimerosal 0. 48 1. 40 0.51 0. 58 ND 0. 75 ± 0. 04b ND 0. 56 ± 0. 10 ± ± ± ± 0. 06 0. 15a 0. 08c 0. 14 a P < 0. 005 ; b P < 0. 05 compared to the control condition, ... 17% of control with 500 instead of 50 nM InsP3 c Relative to corresponding control value at 5 50 nM [Ca2+] d P < 0. 01 compared to control conditions those used by InsP3, in a way insensitive to ... samples of resting and activated platelets (1 80 lL, 3.5 · 108 cells) Cellular activity was stopped by addition of 75 lL ice-cold 10% (w /v) HClO4 After standing on ice for 30 and centrifuging at 200 0...
... 800 lgÆmL)1 G418 (Invitrogen BV, the Netherlands) or 400 lgÆmL)1 hygromycin (Invitrogen BV, the Netherlands) or 400 lgÆmL)1 Zeocin (Invitrogen BV, the Netherlands) Three individual transfections ... Damaj, B ( 200 0) Combinatorial gene expression using multiple episomal vectors Gene 243, 187–194 14 Johansen, F., Braathen, R & Brandtzaeg, P ( 200 0) Role of J chain in secretory immunoglobulin formation ... use in expression vectors Curr Opin Biotechnol 10, 458–464 38 Van, C.K., Vanhoenacker, P., Duchau, H & Haegeman, G ( 200 0) Molecular integrity and usefulness of episomal expression vectors derived...
... Ethanol ( 100 ppm) Ethanol ( 600 ppm) CO ( 10 ppm) CO ( 30 ppm) MoO3 MoO3±WO3 WO3 6.7 10. 0 2.5 14 .0 53 .0 7 .0 1 .05 1 .0 1 .0 1.25 1 .0 1 .0 Conclusions MoO3 based sensors showed promising O3 gas sensing characteristics ... a response of 2.3 and a time response of 60 s to ppm of NO2 as shown in Fig 4.5 Ethanol vapor sensing The sensors where exposed to 100 ± 600 ppm of ethanol and to 10 30 ppm of CO to investigate ... sensors [16] have a response of 10 500 ppm (0. 05%) of ethanol Fig The dynamic response of a MoO3±WO3 film to CO and NO2 2 80 K Galatsis et al / Sensors and Actuators B 83 ( 200 2) 276±2 80 Table Ethanol...
... 505 0 203 3), and the Chinese Academy of Sciences References À1 the current density of 20 mA g (0. 1 C) between 1 .0 and 4 .0V vs Li/Li+ In the discharge curve of the first cycle, there are two obvious ... (2.6–2.75 V and 2 .0 1 .0V vs Li+/Li) During the second discharge, only one discharge slope is observed in the range of 2 .0 1 .0 V, with a decrease of the discharge capacity This change in potential ... S.X Dou, Angew Chem Int Ed 43 ( 200 4) 4212–4216 ARTICLE IN PRESS Z Gu et al / Journal of Solid State Chemistry 1 80 ( 200 7) 98– 105 [8] (a) X.F Duan, C.M Lieber, Adv Mater 12 ( 200 0) 298; (b) C.J Barrelet,...
... fabricating singlecrystalline b-FeOOH hollow nanorods with length in the range of 70 1 10 nm and width in the range of 20 30 nm In particular, a process mechanism has been revealed for synthesis of single- crystalline ... only one big cavity in each particle with length of 50$ 70 nm and width above 10 nm The Fast Fourier Transform (FFT) image in the inset indicates the single crystalline nature of the single hollow ... No 80 17 70, Fe8O8(OH)8Cl1.35, a kind of b-FeOOH, Akaganeite, a=1 .06 0 nm, b =0. 303 4 nm and c=1 .05 1 nm) SEM, TEM and HRTEM images of the final product are shown in Figure The center portion of structure...