... While formality and completeness of typed user -adviser dialogues resemble more Formal Written language, the general syntactic features of typed user -adviser dialogues resemble more Informal ... user -adviser dialogues have some features of planned, Formal Written language but also have features of unplanned, Informal Spoken language. Table 4: Distribution of Propositional Attachments CtHmp~x ... Academic Press. Sager, N. (1982). Syntactic Formatting of Science Infor- mation. In R. Kittredge -~ J. Lehrberger (Eds.), Sublanguage: Studies ofLanguage in Restricted Semantic Domains. New York:...
... coupling constant of thespectrum of the turnover form of the T2Cu center areslightly different from that of the as-purified form (cf.Table 2) which suggests the existence of modifications in ... recent model for the catalytic mechanism of Cu-Nir supposes that nitrite binds to the oxidized form of the T2Cu center, displacing a solvent molecule and forminga hydrogen bond between one of its ... 1.7.2.1).*Present address: Institute of Pharmacology and Therapeutics, Faculty of Medicine of Porto, and Institute for Molecular and Cell Biology,University of Porto, Alameda Prof. Hernaˆ ni Monteiro,4200–319...
... theevent of metal ion donation will be of significant value for understanding the role of MT in the cellular meta-bolism of Zn2+. Despite the relatively large thermo-dynamic stability of the ... side of Fig. 3 clearly show the formation of inter-mediate Cd1a,Cd2a and Cd3a species, with the Cd4aspecies forming only after > 3 equivalents of Cd2+have been titrated. Addition of ... fold-ing of MT results in the formation of discrete metal–thiolate clusters within each of the a and b domainswith stoichiometries of [M4(Scys)11] and [M3(Scys)9],respectively, for...
... is beginning to be perceived. For instance, one striking example of c-Src location insideone organelle has been reported for the inner mem-brane of mitochondria of osteoclasts [57]. Moreover,there ... InMadin–Darby canine kidney (MDCK) cells, a study of chimeric forms of DPP IV has shown that the luminaldomain of DPP IV carries dominant apical sortinginformation while the short cytoplasmic tail ... concentration of thesoluble form of DPP IV may result in an increasedproteolysis of GLP-1 peptides and thus decreased insu-lin secretion [20]. The insulin-dependent phosphoryla-tion of DPP IV...
... spans of TatA and TatBDeletion of the TM spans of TatA or TatB leads to aloss of activity [31] but the important characteristics of these regions have not been probed. We constructeda series of ... 2267 Evidence for interactions between domains of TatAand TatB from mutagenesis of the TatABC subunits of the twin-arginine translocaseClaire M. L. Barrett and Colin RobinsonDepartment of Biological ... effects of substituting groups of basic residues inthese regions of TatA and TatB [24]. The present studyhas increased our understanding of this region in twoways. First, the removal of three...
... </number></MorphoFeat> </hasMorphoFeat> </InflectedForm> </hasInflectedForm> <hasInflectedForm> <InflectedForm rdf:ID="star"> <hasMorphoFeat><MorphoFeat ... </number></MorphoFeat> </hasMorphoFeat> </InflectedForm> </hasInflectedForm></LemmatiedForm>Figure 2: Formalization of the morphological layer and excerpt of a sample ... be a conjunction of Ewiand Ewj,and the counterpart is the intersection of set of translations for each language. This oper-ation would reduce the number of words of each language. That means,...
... conducted in the absence of Tween 80. Finally, in thepresence of Tween 80, oxidation of a-keto diarylpropane (X)resulted in the formation of 3,5-dimethoxybenzoic acid (XII,5.0%) ... Oxidativedecomposition of malonic acid as basis for the action of peroxidase in the absence of hydrogen peroxide. FEBS Lett. 434,362–366.292 G. V. B. Reddy et al. (Eur. J. Biochem. 270) Ó FEBS 2003Cleavage of ... aromatic ring toform an aryl cation radical [3,11,15,16]. Chemical and ESRspectroscopic evidence have confirmed the formation of cation radical species in the LiP-catalyzed oxidation of alkoxybenzenes...
... methylation of the C-terminalhalf of the a1(I) resulted in either reduced binding for a1(I)CB7, or a complete l oss of the binding for a1(I) CB6. Thevariation of the binding ability for N-methylated ... were analyzed for purity bymeans of a quantitative Hyp assay [38], electrophoresis indenaturing conditions [34], N-terminal sequencing for somepeptides and for conformation by means of CD spectro-scopy.Chemical ... Role of decorin on in vitro fibrillogenesis of type I collagen. GlycoconjJ. 14, 871–874.33. Bradford, M.M. (1976) A rapid and sensitive method for thequantitation of microgram quantities of protein...
... were subjected toone cycle at 95 °C for 2 m in; 40 cycles at 95 °Cfor30s,60 °C for 60 s and 72 °C for 90 s ; and a ®nal additionalextension period at 72 °C for 5 m in. Ampli®ed DNAfragments ... 2002understanding of the function and organization of theenzyme complex, the analysis of mutant a lleles of the ge nesinvolved in the biosynthetic pathway c an certainly providevaluable information.In ... activity of this mutant enzyme could not be restoredby the addition of Tween 40 [39], i t can be concluded thatthe 482 Gly residue is important for the activity of theenzyme, likely being one of...
... literature, espe-cially for more advanced stages of melanoma [179] (seeTable 7).An Italian cohort of 8,984 women was followed for anaverage of 9.5 years, with 207 incident cases of breastcancer ... increased risk of colorec-tal cancer [30]. In a study of a cohort in Washingtoncounty, Maryland, increased risk of colorectal cancer wasBioMed CentralPage 1 of 21(page number not for citation ... vitamin D, 25(OH)D, into the active hor-monal form, 1,25(OH)2D. So, there is a local mechanismin many tissues of the body for converting the form of vitamin D in the body that is elevated by...
... properties,providing kinetic evidencefor the direct complex formation of P450nor with NADH.Materials and methodsMutagenesis and expression plasmidsThe construction of each mutant of P450nor of F. oxyspo-rum ... omposition of Ithat follows its formation, although the rate of decomposition is m uchslower than that of I formation (cf. Figure 6).Fig. 5. Saturation kinetics observed o n the reduction (I formation) ... eduction of the D393A mutant is performed withNADH at a higher con centration, the formation of I shouldbe more complete, affording a clearer isosbestic point.Saturation kinetics of the intermediate...
... promis-cuous receptor. Evidence for the conservation of the mechanism of mesoderm induction in molluscs and acrossbilateriansAlthough Lartillot and colleagues have observed for a number of developmental ... Marseille) for provision of the pRN3 plasmid. We acknowledge J. Laisney andA. H. Hansen for their technical assistance. We alsothank Prof Manfred Schartl (Physiological ChemistryI, University of Wuerzburg, ... major group of bilateriananimals, have remained in the background of the molecular survey of metazoan development. We report the cloning and functional study of thecentral part of the BMP pathway...
... the carbonyl oxygen of G168. Inclu-sion of F221 obscures the local structure in the proximity of T171and for clarity is omitted.Table 1. Comparison of functional parameters for HRPC proximalpocket ... type [16,17]. The pKa for the alkalinetransition being similar to that of the wild type, % 11.1[18]. Finally, comparison of the X-ray structures of theoxidized forms of the native [5,19] and ... state of the enzyme. Wehypothesize that this residue imposes a degree of rigid-ity to the structure of the reduced state of class IIIperoxidases.Results and DiscussionTable 1 shows some of...
... Barbanti-Brodano, G., Russo, G. &Negrini, M. (2000) Low frequency of alterations of the a(PPP2R1A-) and b (PPP2R1B) isoforms of the subunit A of theserine-threonine phosphatase 2A in human neoplasms. ... reaction was performed using primers containingthe translation stop of the different isoforms to obtainspecific cDNA. After purification of this cDNA, semiquan-titative PCR was performed. PCR products ... &Goris, J. (1995) Molecular cloning and developmental regulation of expression of two isoforms of the catalytic subunit of proteinphosphatase 2A from Xenopus laevis. Biochem. Biophys. Res.Commun....
... preferences of thelectins for agalactosylated N-glycans. The specificity toagalactosylated N-glycans should help our understand-ing of the previously unknown mechanism of the func-tions of the ... theabsence of DC-SIGN–Fc. As shown in Fig. 9B, the threemajor bands (i.e. 1, of 160 kDa; 2, of 75 kDa; and 4, of 55 kDa) were present on a silver-stained gel, as well as anextra band (band 3, of 65 ... the DC-SIGN-relatedlectins for PA-glycans.Fig. S1. Schematic representation of 157 oligosaccha-ride structures used for FAC analysis.Fig. S2. Structural formulae of Man9GlcNAc2-MTX,Mana1-3Man-PA...