... gene-expression profiling of 4- and 9-h treated and untreated MP consisted of 98 experimental samples Subsequent low-stringency filtering (80% gene presence across all experiments and removal of genes ... retention of phagocyte properties and promoting tissue remodeling Surprisingly, genes of the IL-1 family and its receptors, TNF and IL-6 were consistently upregulated by the alternative class of cytokines, ... 1.E+08 Figure IFN-γ and IL-4 Induction of gene expression by stimulation of LPS-activated MPs with Induction of gene expression by stimulation of LPS-activated MPs with IFN-γ and IL-4 MPs obtained...
... cellulose and with cellobiose (the main product of cellulolysis), and with a mixture of glucose and cellobiose Cell extracts and extracellular media were analysed by 2D NMR spectroscopy and by TLC ... rates of their utilization were 0.10 lmolÆmg of protein)1Æmin)1 and 0.06 lmolÆmg of protein)1Æmin)1, respectively, while the rates were 0.05 lmolÆmg of protein)1Æmin)1 and 0.03 lmolÆmg of protein)1Æmin)1 ... mixture of the two sugars (Fig 3C) Note that, for clarity, ‘MD’ is used here for both maltose and MDs of larger size, and that, similarly, MD1P is used for both maltose-1P and MD1P of degree of polymerization...
... between TM3 and TM4 of mouse and rat UCP3 (Lilly) C-Terminus of human UCP3, AB3046 (Chemicon) C-Terminus of human UCP3, AB3046 (Chemicon) C-Terminus of human UCP3, AB3046 (Chemicon) C-Terminus of human ... 88% Expression of UCP3 protein in SS and IMF mitochondria of various muscles Figure 3A illustrates the distribution of UCP3 in SS and IMF mitochondria obtained from different types of mouse muscle, ... specific and total levels of COX protein were not significantly different in IMF and SS mitochondria The yield in mitochondria, which was determined by comparing the level of COX protein in the sum of...
... chemotaxis, and cell adhesion) and proteolysis, and certain metabolic and signal pathways To validate our microarray findings at different phase of AA, we performed q-PCR on a set of randomly selected ... Bhsp65-tolerized rats showed downregulation of Th1 and Th17 (pro-inflammatory) response, andof other mediators of inflammation and angiogenesis, but of the upregulation of IL-10 (anti-inflammatory/ immunoregulatory) ... expression profile of LEW rats in the preclinical phase of AA The analysis of Bhsp65-induced gene expression profile of rats in the Inc phase of AA mostly reinforced the immune-based and inflammatory...
... performed with a cell density of % · 105 cells per mL Preparation of FeC-Tf and ferric-saturated Tf (Fe2-Tf) Monoferric- (selective loading of Fe3+ in the C-lobe of Tf) and diferric-Tf were prepared ... Binding of Yb3+ to apo-hTf ¼ ½Yb3þ total À ½Yb3 Æ Tf The thermodynamics of binding of Yb3+ to apoTf Binding of Yb3+ to apo-Tf was investigated by addition of aliquots of Yb3+ to a solution of apo-TF ... a solution of apo-Tf containing 2.5 mol equiv of Yb3+, a new broad band centred at 465 nm appeared and increased in Fig The dependence of e (top) and k (bottom) on the molar ratio of Yb3+ to...
... 2C), and the response isotherm gave a [A]0.5 of 168 lm Although Grp78 ⁄ BiP and Grp94 are the two major recipients of the pool of ATP translocated into the lumen of ER [24] the effects of ATP ... ðtheoreticalÞ ¼ ðMr;analyte =Mr;ligand ÞnðAÞRimmobilized ð1Þ where Mr,analyte and Mr,ligand are the relative molecular mass of the analyte and ligand, respectively; n is the number of analyte-binding sites ... 640 lM of VSV8 and a global Rmax(obs) of 27 RUÆ(ng proteinÆmm)2) (C) Double-reciprocal plot of the equilibrium-binding isotherms obtained in the absence of ATP ⁄ Ca2+ (d), in the presence of 500...
... contributions RS, NA and SN contributed to the concept and design of the study, and to the manuscript writing SN, RS and AA performed isolated of monocytes and culture of macrophages RS and AA performed ... exposure of macrophages to 50 μg/mL of either PHT or HPPH prior to LPS stimulation prevented a significant increase in MMP-9 and TIMP-1 (Fig 1D and Fig 2) Levels of Figure The effect of phenytoin, ... Serra et al Journal of Inflammation 2010, 7:48 http://www.journal-inflammation.com/content/7/1/48 Page of 10 Figure The effect of phenytoin, HPPH and LPS on levels of (A) TNF-a and (B) IL-6 in conditioned...
... contributions RS, NA and SN contributed to the concept and design of the study, and to the manuscript writing SN, RS and AA performed isolated of monocytes and culture of macrophages RS and AA performed ... exposure of macrophages to 50 μg/mL of either PHT or HPPH prior to LPS stimulation prevented a significant increase in MMP-9 and TIMP-1 (Fig 1D and Fig 2) Levels of Figure The effect of phenytoin, ... Serra et al Journal of Inflammation 2010, 7:48 http://www.journal-inflammation.com/content/7/1/48 Page of 10 Figure The effect of phenytoin, HPPH and LPS on levels of (A) TNF-a and (B) IL-6 in conditioned...
... treatment plants, and sewage treatment plants one each from the upstream of Andong area to downstream of Busan area of Nakdong river basin Estrogenic activity and concentration of estrogenic substances ... time of reaction (min), v: volume of culture used in assay (ml), OD420: absorbance at the end of reaction, OD570: light scattering at the end of reaction, OD595: cell density at the start of the ... Andong Daegu Busan Andong Waegwan Gangjung Daegu Gumho Riv Goryung Brd Hwang Riv Jeokpo Brd Namji RESULTS AND DISCUSSION Nam Riv Fig shows the sampling points along of Nakdong River in May, and...
... Fertilizer acquisition and relative importance ofdifferentmethodsof acquisition and source of financing of inputs in the 1994/95 season, by program membership 11 Distribution of fertilizer among ... apparent risk and decline in profitability of hybrid maize production because of the devaluation of the Malawi kwacha and the abolition of fertilizer subsidies in 1994/95 The low profitability of hybrid ... of households’ land is owned by the household heads Spouses own only 17 percent of land, and only percent of land is jointly owned by heads and their spouses Overall, spouses own 22 percent of...
... where kA and k0 are the inactivation rate constants either in the presence or in the absence of various concentrations of a ligand A, k2 and k1 are the rate constants of the inhibition of the bilitranslocase– ... detailed in the text and in Fig 5B n, Number of [A] tested; k2 ⁄ k1, the value of the intercept in the Scrutton and Utter plot, where k2 and k1 are the rate constants of the inhibition of either the ... Sigma-Aldrich and Carlo Erba (Milan, Italy), and were of the highest available grade Acknowledgements Thanks are due to Prof G.L Sottocasa and Dr Antonella Bandiera (University of Trieste) for...
... rights of man and the legitimacy of democracy in the human community and relation to the cosmic order Etlin, in his description, is not writing so much on the part of the actual structure of the ... emotion of the building and the metaphorical relationship man has with the contents of the building The example of the National Archives and its meaning as a building is the first major example of ... The civic architecture of Cret is Etlin's prime example Following the ideas set forth by the Beaux-Arts style of Durand, or the grandeur of Blondel, Cret was "a master of symbolic character."...
... latter is of special interest in view of a recent publication on the identification of a testis and brain specific isoform of mouse PDK1, mPDK-1b [29], in which the authors suggest that this isoform ... Ser166 and Thr168 located within a T-loop play a significant role in the regulation of TSSK3 activity and suggest a similar mechanism of activation to that of the AGC kinase family For a number of ... Right: One-dimensional TLC of hydrolysates of 32P-labelled GST–peptides phosphorylated by TSSK3 The positions of standard phosphoamino acids are indicated (D) bands of peptides used in (B, C)...
... (Davidov and Rappoport, 2006; Widdows and Dorow, 2002) and meronymy (Berland and Charniak, 1999; Girju et al., 2006) Since named entities are very important in NLP, many studies define and discover ... number of patterns (b) For each corpus D different from DX : i Scan D for clusters C2 that share at least S percent of their patterns, and all of their core patterns, with C1 ii Add all patterns of ... modify the relative weight of core and unconfirmed patterns Corpora and Parameters In this section we describe our experimental setup, and discuss in detail the effect of each of the algorithms’ parameters...
... independent of the others Then this relation is representing by the following form of rewriting rule of CFG: B =~ A B , where A is the attribute of the anterior bunsetsu and B is that of the posterior ... these numbers Let d be the number of bunsetsu depending on it and v be the number of bunsetsu with comma depending on it, the set of terminal symbols T and that of non-terminal symbols V is represented ... context-free grammar (SCFG) The terminal symbol is the attribute of a bunsetsu, represented by the product of the head of the content part and that of the function part From the attribute, a word sequence...
... (Mr) of 650.32 This Mr value is 17 units less than the sum of the Mr of DCA (335.50) and that of a tripeptide, Gly–Gln–Lys (331.37), the sequence of which corresponds to Gly8–Gln9–Lys10 of histone ... wavelength; right) Lanes and 4, DCA only; lanes and 5, DCA and EDTA; lanes and 6, DCA and MAB Sizes of molecular-mass-marker proteins are shown at the left (B) RP-HPLC profile of DCA-labeled histones ... carried out in the presence of the methyl ester of alutacenoic acid B, a specific and potent inhibitor of transglutaminase [19], at a concentration of 80 ngÆmL)1, the bands were not detected (Fig...
... coordinate of the fiber, α is the attenuation coefficient of the fiber, and Ep, Eprobe and Eidler are the electric field of the pumping, probe and idler waves γ is the nonlinear coefficient, and is ... networks of the future using optical cross connects A number ofmethodsof wavelength conversion have been proposed, of which parametric conversion using optical fiber FWM offers two major advantages: ... measurement with the states of polarization of the probe light and pumping light carefully aligned and without the use of a polarizer (see Figure 3) Measurements of input probe light were taken...
... adipocytes and whole adipose tissue (Figures 2A, B and 2C) Regulation of SLPI expression in adipocytes by inflammatory stimuli and anti-inflammatory effect of SLPI Adipocytes express both TLR2 and TLR4 ... mRNA was determined by RT-PCR and western blotting Both LPS and peptidoglycan increased SLPI mRNA (Figure 3A) and protein (Figure 3B) Bars represent means and ± SEM ofdifferent replicates Superscript ... adipocytes The anti-inflammatory action of SLPI may involve stabilization of IKBa abundance Activation of TLR2 and increased expression of SLPI in macrophages [10,24] and in adipocytes as confirmed in...
... adipocytes and whole adipose tissue (Figures 2A, B and 2C) Regulation of SLPI expression in adipocytes by inflammatory stimuli and anti-inflammatory effect of SLPI Adipocytes express both TLR2 and TLR4 ... mRNA was determined by RT-PCR and western blotting Both LPS and peptidoglycan increased SLPI mRNA (Figure 3A) and protein (Figure 3B) Bars represent means and ± SEM ofdifferent replicates Superscript ... adipocytes The anti-inflammatory action of SLPI may involve stabilization of IKBa abundance Activation of TLR2 and increased expression of SLPI in macrophages [10,24] and in adipocytes as confirmed in...
... proteins and because of unavailability of sequence information for rest of the genome; we speculate that GA98 may be a derivative of the Ark DPI strain Analysis of the structural region of Ark ... number of substitution events Figure genome Schematic presentation of the structural region of Ark DPI Schematic presentation of the structural region of Ark DPI genome Entire genome of Ark DPI ... Jackwood MW: Molecular cloning and sequence comparison of the S1 glycoprotein of the Gray and JMK strains of avian infectious bronchitis virus Virus Genes 1995, 9:219-229 Hofstad MS: Cross-immunity...