adipogenic differentiation of adipose derived stem cells
Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
... release of hematopoietic stem cells from the bone marrow into the bloodstream of patients with a variety of malignancies The stem cells are then harvested from the blood as a source of stem cells ... cells Since an SDF-1 gradient determines the direction of mobilization of hematopoietic stem cells and progenitors [6], we examined whether SDF-1 levels are decreased in bone marrow stromal cells ... bones of TNF-transgenic mice stromal cellsstromal cell -derived factor-1 expression in bone marDecreased stromal cell -derived factor-1 expression in bone marrow row stromal cells and bones of TNF-transgenic...
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Báo cáo y học: "Pro-inflammatory properties of stromal cell-derived factor-1 (CXCL12) in collagen-induced arthritis" pptx
... while they were treated with AMD3100 reduced the number of harvested cells to that of the negative control The number of cells in the air pouch of AMD3100-treated mice after challenge with 0.9% NaCl ... appearance (e) Joints of arthritic AMD3100-treated mice show a weak infiltration of mono- and polymorphonuclear cells and hyperplasia of the synovium (f) Joint section of a PBS-treated mouse, ... score of the entire group Haematoxylin-stained sections showed that the absence of redness and swelling in AMD3100-treated mice corresponded with the absence of infiltration of immunocompetent cells...
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Báo cáo khoa học: Syndecan-4 is a signaling molecule for stromal cell-derived factor-1 (SDF-1)/ CXCL12 pptx
... phosphorylation of CXCR4, tyrosine phosphorylation of SD-4 occurs in response to SDF-1 stimulation of HeLa cells This tyrosine phosphorylation depends on the time of incubation of the cells with the ... hetero-oligomerization of CXCR4 induced by SDF-1 on HeLa cells is prevented by heparitinases I and III pretreatment of these cells Heparitinases I and III pretreatment of the HeLa cells also significantly ... III-treated HeLa cells In parallel, we investigated whether the reduction of expression of SD-4 on HeLa cells by the use of RNA interference prevented these activations HS removal from HeLa cells or...
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Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
... immune system Binding of antigen to the TCR complex results in a cascade of events commencing with phosphorylation of critical domains of the receptor by membrane-associated Src family of protein ... systems Mechanisms of immune evasion include presentation of a tumor antigen by tumor cells without the necessary co-stimulatory signal [23,24], suppression or anergy of tumor-infiltrating T -cells ... anti-CD3 and anti-CD28 Typically, only ~10% of cells (not shown) became infected as judged by expression of GFP, a component of the receptor construct A variety of techniques were tried to increase...
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báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
... immune system Binding of antigen to the TCR complex results in a cascade of events commencing with phosphorylation of critical domains of the receptor by membrane-associated Src family of protein ... systems Mechanisms of immune evasion include presentation of a tumor antigen by tumor cells without the necessary co-stimulatory signal [23,24], suppression or anergy of tumor-infiltrating T -cells ... anti-CD3 and anti-CD28 Typically, only ~10% of cells (not shown) became infected as judged by expression of GFP, a component of the receptor construct A variety of techniques were tried to increase...
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Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx
... Clonal analysis of the differentiation potential of human adipose- derived adult stem cells J Cell Physiol 2006, 206:229-237 Fukuda K: Reprogramming of bone marrow mesenchymal stem cells into cardiomyocytes ... analysis software (SABiosciences) To study the effect of viral exposure on HD cells, we compared the expression of selected genes in HD-HIV cells versus un-infected HD cells The gene profile of HD-HIV ... expression of NFB1A slightly increased as a result of differentiation (Figure 1C) A highly productive HIV-1 infection is evident in virus exposed HD cells Since cells of the hematopoietic system are...
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Cloning of medaka glial cell derived neurotrophic factor (GDNF) and its receptor GFR alpha 1
... Spermatogonial Stem Cells (SSCs) Differentiation of germ cells in the testis originates from a constantly renewed small pool of stem cells, termed SSCs located at the basement membrane of the seminiferous ... all of you as my labmates i TABLE OF CONTENTS ACKNOWLEDGEMENTS I SUMMARY IV LIST OF ABBREVIATIONS V LIST OF FIGURES VII LIST OF TABLES VIII CHAPTER 1: INTRODUCTION 1.1 Spermatogonial Stem Cells ... (1971), these stem cells are represented only by a discrete sub-population of type A spermatogonia cells or more specifically, Asingle (As) spermatogonia and numbered no more than 0.03% of the total...
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báo cáo hóa học:" Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy – a case report" doc
... A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse Cells were cultured and ... cytolytic activity of patient -derived PBMNC derived from L1 to L6 and L7 to L9 against K562 cells (A) and autologous In vitro (B) In vitro cytolytic activity of patient -derived PBMNC derived from L1 ... The presence of CD3+ and CD45+ cells was used as an indicator of T cell infiltration and the prevalence of CD4+, CD8+, CD56+ cells as indicators of T helper, T cytotoxic and NK cells, respectively...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... function of tumor-educated myeloid cells was measured by their ability to inhibit the proliferation of autologous T cells in the following Suppression Assay: T cells isolated from 30 mL of PBMC ... shown from Suppression Assays of CD33+ or CD11b+ cells with autologous T cells, respectively * indicates statistically significant suppression of T cells by CD11b+ cells from co-culture (p
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Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
... therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury of the lung has not been investigated Recently, ADMSCs have been reported to have the distinct advantages of being ... Use of Laboratory Animals (NIH publication No Page of 13 85-23, National Academy Press, Washington, DC, USA, revised 1996) Animal Grouping and Isolation of Adipose- Derived Mesenchymal Stem Cells ... parenchyma from IR damage Figure Impact of Adipose- Derived Mesenchymal Stem Cells (ADMSC) Transplantation on the Severity of IR-Induced Lung Parenchymal Injury Number of alveolar sacs and crowded area...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
... Results Differentiation test of ASCs Adipogenic differentiation of ASCs was apparent after weeks of incubation with adipogenic medium By the end of the third week, half of the cells contained Oil red ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine,...
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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... mechanism of regeneration is not yet clear It could be through direct differentiation of stem cells that were introduced to the diseased joints Alternatively, it could be due to the tropic effects of ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of full-thickness...
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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf
... dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells ... function of DCs Polarization of naïve Th cells into Th1 cells is critical for the induction of cellular immunity against intracellular pathogens and cancer cells The observed impairment of the ... the percentage of positive cells and the mean fluorescence intensity (MFI) of the population were determined FACS sort of lacZ-positive cells The lentiviral siRNA vector-transduced cells co-expressing...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... Detection of smooth muscle cells after differentiation of the BMP2+ cells Figure Detection of smooth muscle cells after differentiation of the BMP2+ cells (a) Expression of SMA in the BMP2+ cells ... expression (e) Figure Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls ... BMP2-expressing cells during early differentiation of ES cells will pave the way for a better understanding of NCSCs and their differentiation into lineages The BMP2+ cells derived from the ES cells may...
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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
... cells [31,32] and other cells of the innate immune system (for example, natural killer cells) , as well as in exerting Genome Biology 2007, 8:R30 information Our finding of negligible levels of ... Figure Phenotypic comparison of cells Phenotypic comparison of cells The expression of cell surface markers on (a) immature and (b) mature mDCs, moDCs and KG1 cells Immature cells were freshly isolated ... model systems, moDCs and KG-1 cells, our findings identified important differences between the proteomes of these cells, and the findings were confirmed by ELISA detection of a selection of proteins...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem cell as a vehicle ... (elaborated in section 3.1) 1.4 Stem cells 1.4.1 Adult stem cells (ASCs) Adult stem cells (ASCs), also known as somatic stem cells, are multipotent undifferentiated stem cells found in a tissue or ... 58 10 LIST OF FIGURES Figure Generation of iPS cells from mouse embryonic fibroblasts (MEFs) 55 Figure Generation of neural stem cells from mouse iPS cells and characterization of derived NSCs...
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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... neuroepithelial-like stem cells for the generation of modified neuronal cultures 59 4.2 Generation of gene-corrected neural stem cells from MJD patient -derived iPS cells 59 4.2.1 ... undergo differentiation into diverse and more restricted progeny All tissues and organs of the body are derived from cascades of stem cells, which become more and more restricted in differentiation...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... the oligomycin-resistant portion of respiration reflects the level of proton leak of mitochondria, the low level of respiration in the presence of oligomycin of L6 cells showed that they were very ... the presence of an uncoupler, such as CCCP, the respiratory activity of Fig Localization of hUCP3 in L6 cells by immunofluorescence L6 cells were seeded at a density of 1.5 · 104 cells cm)2 Infection ... adenovirus particles at the moment of cells transduction Infection of L6 cells with the adenovirus particles was performed with the help of the transfection reagent Lipofectamine Plus (Life Technologies)...
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