adhesion molecules in osteoclasts
Báo cáo y học: " Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung" pot
... we examined BALT T cells and B cells for the expression of L-selectin, which binds to PNAd; α4 integrin, which is a subunit of α4β1 integrin (which binds mainly to VCAM-1) and α4β7 integrin (which ... rinsed in PBS, incubated with biotin-antimouse IgG (to detect CD31, MAdCAM-1 or VCAM-1) or biotin-anti-rat IgM (to detect PNAd) at room temperature for 30 minutes (min), rinsed in PBS, and incubated ... has HEV adhesion molecules that are capable of recruiting distinct subsets of lymphocytes from the bloodstream into the tissue As in mice, the combination of adhesion molecules on HEVs in human...
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Báo cáo khoa học: "Changes in some Blood Micronutrients, Leukocytes and Neutrophil Expression of Adhesion Molecules in Periparturient Dairy Cows" ppt
... were collected in the morning, using Vacutainer® tubes (Becton Dickinson Vacutainer Systems, Meylan, France), one month before estimated calving, at calving (within 24 hours after calving) and one ... significant increase in the numbers of WBC at calving This was mainly due to an increase in the numbers of circulating neutrophils, and to a less extent, an increase in monocytes At calving, the ... neutrophil adhesion molecules were done using the General Linear Model (SAS Institute Inc., Cary, NC, USA) The effects of cow and period were included in the model Mean fluorescent intensity for...
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Báo cáo y học: " Adhesion molecules in different treatments of acute myocardial infarction" doc
... decreasing or increasing intra-individual In patients undergoing PTCA, sICAM-1 levels were significantly elevated in comparison with controls and patients with tPA or SK lysis, whereas no intra-individual ... remained significant during the whole observation period No increasing or decreasing intra-individual trends could be observed, either in the tPA group or in the PTCA group SK patients, in contrast, ... to L-selectin, this mode is unique to L-selectin among adhesion molecules of the selectin or immunoglobulin families, and results in baseline soluble L-selectin concentrations exceeding those...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf
... precipitation of insulin B C Fig aB-crystallin protects against the DTT-induced aggregation of insulin, and this activity is enhanced by Arg-HCl Insulin ( , 0.25 mgÆmL)1) was incubated at 37 °C in 50 mM ... aB-crystallin against this fibril-forming protein, implying that it is the common positively charged group that plays a role in increasing the activity of aB-crystallin against this target protein To ... monitored in real time by incubation at 37 °C in 50 mm phosphate buffer, pH 7.2, without shaking for 15 h Single-point ThT readings were taken for a-synucleinA53T during incubation at 37 °C in 50...
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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
... missing links in insulin signal transduction? FEBS Lett 410, 3–10 Dedhar, S., Benjamin, W & Gregory, H (1999) Integrin-linked kinase (ILK): a regulator of integrin and growth-factor signalling ... that of PKB protein and activity in the insulin treated and S-PKB cells, indicating that SLex might be regulated by other insulin-induced factor(s) in addition to PKB signalling pathways It is ... alteration in PKB protein was similar to that in PKB activity (Fig 7), suggesting that the insulininduced PKB protein was almost fully phosphorylated and activated Whether insulin was internalized...
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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
... [7] To gain a more complete inventory of factors required for the entry of ricin A chain to the cytosol it will be useful to express inducible toxin in the ER of mutant strains of yeast, in a manner ... profile to RTAD, again indicating some difficulty in assuming a tightly folded conformation By contrast, RTAE177D is mainly non-glycosylated with only a minor fraction carrying a single glycan This ... present, but the emerging picture indicates that toxins like ricin can exploit an unknown number of ER and membrane components normally involved in perceiving and extracting proteins from the ER to...
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Báo cáo khoa học: Upregulation of endothelial adhesion molecules by lysophosphatidylcholine pptx
... GPR4, emphasizing the involvement of GPR4 in LPC-induced AMs, thereby providing a new insight into the molecular mechanism of LPC’s role in the expression of AMs in ECs The LPC-induced increase of ... eliciting second messenger cAMP in several cell types [23–25], so the levels of cAMP were determined in LPC-challenged YPEN-1 cells As indicated in Fig 4B, a 15-min incubation with LPC at increasing ... of insulin [15] We are currently examining the possible participation of GPR119 in LPC-induced AM expression The data from the present study indicated that downstream signaling following LPC-induced...
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atoms and molecules in strong external fields
... decreasing the radius and increasing the density? That depends on how the two pressures in the balance react to increasing density using again the equation of state for an ideal gas In the beginning ... projecting onto the we obtain the following system of coupled partial differential equations for the functions of the internal coordinates Those functions were determined by expanding them in terms ... the evolution from ordinary stars to NSs, including the collapse This assumption results in typical fields depending on the field in the (ordinary) progenitor star, According to radio pulsar models,...
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Báo cáo y học: "RANKL increases the level of Mcl-1 in osteoclasts and reduces bisphosphonate-induced osteoclast apoptosis in vitro" pps
... in osteoclasts for continual protein synthesis to prevent apoptosis [41,45] Together, these observations indicate that Mcl-1 plays an important role in maintaining osteoclast survival Mcl-1 inhibits ... BPs such as CLO and ALN) in preventing local, inflammation-induced bone loss in RA 11 Competing interests MJR has received research funding from Procter & Gamble (Cincinnati, OH, USA), Novartis ... RANKL prevents BP-induced apoptosis, we examined in more detail the events involved in ALN-induced apoptosis Co-treatment with RANKL prevented the increase in the proportion of osteoclasts with...
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báo cáo khoa học: "Malignant papillary peritoneal mesothelioma presenting as recurrent adhesion obstruction in general surgery: a case report" potx
... abdominal CT scan examination may show the presence of ascitic fluid and peritoneal thickening [5], and calretinin immunostaining of ascitic fluid can be done This procedure has significantly increased ... but in peritoneal tuberculosis the lesions are white with surrounding erythema and normal-appearing intervening serosa In adhesions, the bands are obvious and the bowel serosa is normal In the ... writing the article MJR wrote and edited the manuscript CLL assisted in researching the literature and writing the paper Competing interests The authors declare that they have no competing interests...
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Báo cáo y học: "Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis" docx
... HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric ... cells [16] Galectin-9 has been shown to induce apoptosis of T cells via the calcium-calpain- caspase-1 pathway [17] In mice, interference with the TIM3-TIM-3-ligand binding resulted in hyperproliferation ... observed in BALF versus blood (in patients, p = 0.004; in controls, p = 0.03) The lines indicate T cell subpopulations from the same individual * p < 0.05, ** p < 0.01 was down-regulated in MS [25] Indeed,...
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Báo cáo y học: "Abnormal networks of immune response-related molecules in bone marrow cells from patients with rheumatoid arthritis as revealed by DNA microarray analysis" ppsx
... was intraoperatively obtained from the nine RA patients and the 10 OA patients while undergoing joint arthroplasty Detailed patient characteristics, medication usage, including disease-modifying ... maintenance of chromosome (SMC)-related molecules, and two kinesin family member (KIF)-related molecules are also represented in the second network CCNE1, CDK inhibitor 1B, and histones are involved ... serum IL6 in BM has been reported in RA patients [10] This is important because IL6 induces the secretion of hepcidin, a humoral factor regulating intestinal iron absorption and iron storage in microphages...
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Báo cáo y học: " The predictive role of serum and bronchoalveolar lavage cytokines and adhesion molecules for acute respiratory distress syndrome development and outcome" pptx
... p44/42 mitogen-activated protein kinase (MAPK) pathway and the phosphatidylinositol 3-kinase (PI 3-kinase) pathway [8] Insulin initially binds to the insulin receptor causing autophosphorylation of ... human lung adenocarcinoma cell line, NCI-H441, was maintained in vitro in monolayer culture in 10% fetal bovine serum in the presence of penicillin (100 U/ml), streptomycin (100 µg/ml) and fungizone ... kinase was increased in the presence of insulin after 15 of incubation and remained increased after 16 hours Rapamycin abolished the phosphorylation of Thr389 in both control and insulin-treated...
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Báo cáo y học: " The predictive role of serum and bronchoalveolar lavage cytokines and adhesion molecules for acute respiratory distress syndrome development and outcome" doc
... found to be increased in BALF in the early phase of ARDS, including endotoxin-binding proteins, tumour necrosis factor alpha (TNF-α), interleukin (IL)-1, IL-6, chemokines, adhesive molecules as ... Discussion We investigated two categories of soluble molecules that might be involved in the pathogenesis of ARDS: proinflammatory cytokines and adhesion molecules We studied these molecules within the ... survival in all groups Conclusion In conclusion, our data show that the two groups of molecules (proinflammatory cytokines and adhesion molecules) studied in a single measurement, within hours...
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Báo cáo y học: "Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules" ppt
... II alveolar epithelial cell line, and NCI-H292, a tracheal epithelial cell line, were maintained in RPMI 1640 containing 10% fetal bovine serum (FBS) MT-2 cells, obtained by coculture of peripheral ... resulted in infection with HTLV-I Figure of expression of cytokines, chemokines and cell adhesion molecule in A549 cells cocultured with MT-2 cells Induction Induction of expression of cytokines, ... lymphocytic interstitial pneumonia [7,8] In Tax-expressing transgenic mice, inflammatory cells consisting mainly of lymphocytes accumulate in peribronchiolar and perivascular areas as well as in alveolar...
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Light sheet based fluorescence correlation and cross correlation spectroscopy for quantitative measurements of bio molecules in live cells
... players in biological systems to enable understanding in protein-protein interactions or protein-DNA interactions A number of techniques, including protein microarrays, pull down assays and chromatin ... circumvented in spinning disk confocal FCS by scanning widely separated pinholes over the sample [40] However, in this case the detection efciency is reduced, since each pinhole resides at each point ... imaging method can provide spatio-temporal dynamics and binding maps of proteins localized in membrane, cytosol and nucleus This particular study combines fast SPIM imaging (single plane illumination...
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Rovibrational analysis of asymmetric top molecules in vibrationally excited states by high resolution FTIR spectroscopy
... parameters occurring in it are to be determined Problems of this type tend to be indeterminate, because the eigenvalues may depend only on certain combinations of the parameters The parameters in S must ... handling system 2.2 Dynamic Alignment During a scan, the moving mirror has to move in a highly parallel manner In order to achieve this and also to overcome the difficulty of getting an optimum initial ... and after substituting it into S1(ν), the output of a monochromatic input for finite path differences is Ap1(ν,νo) = 2L{sinc[2π(ν − νo)L + sinc[2π(ν + νo)L]} [1-9] which is a sinc function with...
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Systematic study of hadronic molecules in the heavy quark sector
... Leading (a) and subleading (b) contributions to light meson-scattering in ChPT 2.4.1 Chiral Perturbation Theory For the power counting in ChPT we have in principle to distinguish between two ingredients: ... shown in Fig 2.2 Expanding Eq (2.23) to leading non-vanishing order we find the correct spin-0 kinematic terms for the Goldstone bosons Parallel to the expansion in terms of momenta we can also incorporate ... are degenerate To incorporate the fact that this is not realized perfectly in nature we introduce the leading SU(3) and spin symmetry breaking terms The first spin symmetry breaking term appears...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
... (GO:0007166) The basigin protein was shown to be present in vascular endothelium in both in the non-neoplastic region of the brain and in tumor cells (http://amigo.geneontology.org/cgi-bin/amig o/gp-details.cgi?gp=UniProtKB:P35613) ... Software and are described in the following part according the definitions of the Illumina’s (ILL) data as well as of the Illumina’s annotations (table 2) http://www.medsci.org Int J Med Sci 2009, 22 ... Its cytoplasmic gene product is a serine/threonine-protein kinase N1 which is a member of the protein kinase C superfamily (http://amigo.geneontology.org/cgi-bin/amigo/ gp-details.cgi?gp=UniProtKB:Q16512)...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... the increase in the prooxidant state caused by iron loading has an impact in protein integrity, as indicated by an increase in protein-bound acrolein adducts at the cell surface, which point ... ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured without and with iron as indicated (D) Perls’ staining of cytospins ... as determined by the increase in mean fluorescence intensity (MFI) (Fig 1C) As shown in Fig 1B, permeabilization with saponin did not increase background staining when rabbit immunoglobulins were...
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