adhesion and osteogenic differentiation of human mesenchymal stem cells

Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf

... Materials and methods [above]) The relative number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic ... osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression during osteogenic differentiation of human MSCs ... bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 J Cell Biochem 1997, 67:386-396 Jager...

Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells

Danh mục: Thạc sĩ - Cao học

... 2.20 Representative SEM images of nanofibers fabricated by different techniques: (A) Random nanofibers fabricated by electrospinning, (B) Aligned nanofibers fabricated by electrospinning (Adapted ... native bone (bone structure, bone cells, bone formation and bone repair), bone grafts and available bone graft substitutes, stem cell response to biomaterial topography, the importance of nanofibers ... craniofacial bones and the lateral part of clavicles, are formed by intramembranous ossification, whereas the long bones of the limbs, basal part of the skull, vertebrae, ribs and medial part of...

Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Danh mục: Cao đẳng - Đại học

... expand human mesenchymal stem cells using basement membrane extracellular matrix Biochem Biophys Res Commun (3):503-508 60 Chen XD, Dusevich V, Feng JQ, et al 2007;Extracellular matrix made by bone ... 1997;Differential binding of fibroblast growth factor-2 and -7 to basement membrane heparan sulfate: comparison of normal and abnormal human tissues Am J Pathol (4):1443-1455 67 Yayon A, Klagsbrun M, Esko ... differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express vascular cell phenotypes upon...

Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Danh mục: Báo cáo khoa học

... space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by the appearance of hypertrophic cells ... (basic concepts) J Tissue Eng Regen Med 2008, 2:1-13 43 Bandyopadhyay A, Tsuji K, Cox K, Harfe BD, Rosen V, Tabin CJ: Genetic analysis of the roles of BMP2, BMP4, and BMP7 in limb patterning and ... ethidium bromide and visualized using the Bio Profile software (LTF, Wasserburg, Germany), allowing correlation between EF1α signals and cycle number for each sample The densities of the PCR bands...

Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx

Danh mục: Báo cáo khoa học

... from mesenchymal stem cells in Wharton’s Jelly of the human umbilical cord for transplantation to control type diabetes PLoS ONE 2008, 3(1):e1451 Liu M, Han ZC: Mesenchymal stem cells: biology and ... in enhancing IPC differentiation by FN and LAM, the cells were pretreated with LY294002 (a specific inhibitor of PI3 Kinase) or PD98059 (a specific inhibitor of MEK 1) followed by induction with ... other hand, PD98059 decreased the activation of ERK both in cells treated with FN or LAM (Figure 5B and 5D) and increased the activation of Akt about 2.7-fold and 2.1fold compared to FN and LAM...

báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Danh mục: Hóa học - Dầu khí

... green I6, H9 and BG01v are names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day ... cells, expression of OCT4 in hES was increased by 4,324-fold, LEFTY1 by 769-fold, and TDGF1 by 2,443-fold We did not find that Nanog was upregulated in hES cells by microarray analysis, and by ... hematopoietic stem cells (HSCs) from human umbilical cord blood, and it promotes differentiation of HSCs into progenitor cells by inhibiting ABCG2 expression[56] Page 10 of 17 (page number not for...

Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages

Danh mục: Tổng hợp

... mark the cells overlying both embryonic and extraembryonic regions and are subsequently restricted to the visceral layer of the yolk sac (Weber et al 1999; Beddington and Robertson 1999) By the ... immunosuppressive drugs like tacrolimus (binds calcineurin and thereby inhibits T-cell signaling ) and sirolimus (blocks 11 activation of T- and B- cells by inhibiting interleukin-2 responsiveness) that prolonged ... form of blood islands in the yolk sac, a derivative of the extraembryonic mesoderm by embryonic day E7.5 of gestation (Russell and van den Engh 1979) This is preceded by the expression of key...

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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders

Danh mục: Cao đẳng - Đại học

... of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated ... the various types of skin cells Some recent reports suggest that adult stem cells such as haemopoietic stem cells, neuronal stem cells and mesenchymal stem cells could cross boundaries and differentiate ... characteristics and behaviour to embryonic stem cells served as a model to isolate comparable cells from mammalian embryos The first report on the growth of ICMs and the isolation of stem cells from human blastocysts...

Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt

Danh mục: Báo cáo khoa học

... ASB/SBP ni detulid erew seidobitna ehT seidobitna yramirp fo gnidnib cificeps-non eht kcolb ot Co73 ta nim 001 rof )ASB/SBP( ASB %4 gniniatnoc SBP ni )ASU ,demyZ( mures taog %2 dna )ASU ,LRB-ocbiG( ... eulb napyrt gnisu yb dna retemotycomeh a htiw denimreted erew sllec elbaiv dna detaelcun fo srebmun latot ehT )ASU ,LRB-ocbiG ;SBF( mures enivob latef %01 htiw detnemelppus saw taht )ASU ,LRB-ocbiG( ... 2-ateb dna 1-ateb fo noitanibmoc a aiv amorts worram enob ot dnib sllec aimekuel dioleym etuca namuH JD beilttoG ,K lepeltroK ,JL lladneB secnerefeR ecneicS yranireteV rof margorP 12 aeroK niarB...

Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt

Danh mục: Báo cáo khoa học

... RT-PCR analysis of expression levels of hTERT and GAPDH, and (B) Real-time RT-PCR analysis of expression levels of DNMT1, DNMT3A and DNMT 3B in primary human mesenchymal stem cells transfected ... cultured human embryonic stem cells Nature genetics 2005, 37:1099-1103 43 Takahashi K, Yamanaka S: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined ... cell body with bipolar elongated cell processes, and by RT-PCR after induction with noggin for 14 days (C) Cobble stone formation by co-culture with hematopoietic stem cells (D) Trophoectoderm- and...

Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx

Danh mục: Báo cáo khoa học

... J, Gimble JM, Bunnell BA: Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue J Cell Biochem 2006, 99:1285-1597 Boyer LA, Plath K, Zeitlinger J, Brambrink ... at least doubled the number of CFU-Fs present while enhancing the expression of oct-4 and rex-1, genes expressed by embryonic stem cells and thought to be pivotal in maintaining ‘stemness’ These ... FGF, fibroblast growth factor; CFU-F, colony forming unit-fibroblast; c, chondroblast; o, osteoblast; a, adipoblast; m, myoblast; cm, cardio-myoblast; t, tenoblast and FGF10 feedforward loop between...

Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1

Danh mục: Y - Dược

... ANOVA Analysis of Variance BESTT BMP-2 Evaluation in Surgery for Tibial Trauma BM Bone Media BM-MSC Bone-marrow derived MSC BMP Bone Morphogenetic Protein BSA Bovine Serum Albumin BTE Bone Tissue ... potential in BTE applications Table 2-9 Summary of the main characteristics of mature endothelial cells and its progenitor cells Table 2-10 Comparison between UCB and adult PB-EPC in terms of its colony ... control, a biotinylated protein as indicated by the manufacturer Table S5 Osteogenesis Genes (shown for UCB x PB), fold difference between UCB-EPC and PB-EPC (n=3), LN2 Values given for UCB and PB-EPC...

Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Danh mục: Báo cáo khoa học

... Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin ... factor (LIF) These cells express the Rex-1 and b- actin genes but not the embryonic and adult globin genes (Fig 2) Upon differentiation the embryonic- and adult-speciﬁc b- globin genes are sequentially ... nature and extent of covalent histone modiﬁcations and association of transcription complexes at the murine b- globin locus during the in vitro differentiation of murine embryonic stem cells We observed...

báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Danh mục: Báo cáo khoa học

... individual lines may be a big contributor to the reported DMRs However, a significant subset of DMRs is shared among iPSC lines of different genetic background and cell type of origin, and is transmitted ... megabase-scale regions of reduced methylation can be detected in iPSCs, often within the vicinity of centromeres and telomeres [90] Biased depletion of DNA methylation from subcentromeric and subtelomeric ... fraction of the human genome can be significantly reduced by defining the regulatome of a given cell type via epigenomic profiling of chromatin signatures that define different types of regulatory...

Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc

Danh mục: Báo cáo khoa học

... immunomodulatory activity of human bone marrow mesenchymal stem cells Stem Cells 2006, 24:386-398 Meisel R, Zibert A, Laryea M, Gobel U, Daubener W, Dilloo D: Human bone marrow stromal cells inhibit allogeneic ... http://arthritis-research.com/content/12/1/R31 Page of 11 Figure Phenotype and differentiation potential of mesenchymal stem cells (MSCs) Bone marrow cells of DBA/1 wild -type and interferon-gamma receptor knockout ... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of...

Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot

Danh mục: Báo cáo khoa học

... Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Wuertz K, Godburn K, Neidlinger-Wilke C, Urban J, Iatridis JC: Behavior of mesenchymal stem cells in the chemical ... http://arthritis-research.com/content/13/3/R81 Page of 13 Table Study design for treatment of MSCs with B, C, NCA and NCT groups Media group Number of human samples Time in culture PCR GAG Cell viability Alcian blue stain B 21 days C ... source for IVD repair, as described by a number of in vitro and in vivo studies [10-19] The interaction between MSCs and cells of the native IVD, including the adaptation of MSCs to the IVD microenvironment,...

Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28

Danh mục: Thạc sĩ - Cao học

... Summary V List of Tables VII List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory ... SAGE_Skin_melanoma _B_ DB1 Undifferentiated HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE_Embryonic _stem_ cell_H9_normal_p38_CL_SHES1 LSAGE_Embryonic _stem_ cell_BG01_normal_p20_CL_SHE19 ... wingless -type MMTV integration site family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are...

Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Danh mục: Y học thưởng thức

... Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... pre-implantation embryos of mice (8-cell and older) can be shipped overnight at room temperature with no apparent ill effects, the same cannot be said of human embryos, which are much less robust and hardy ... all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells were washed off prior to the MTT assay From the raw absorbance...

Atlas of Human Pluripotent Stem Cells doc

Danh mục: Sức khỏe giới tính

... amenable for long-term culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the ... ﬁrst IVF test-tube baby in 1978 (Steptoe and Edwards 1978) and the discovery in 1981 by Martin (1981) and Evans and Kaufman (1981) of mouse embryonic stem cells Later, development of sequential ... Atlas of Human Pluripotent Stem Cells Derivation and Culturing With contributions by Ilana Laevsky, BA and Atara Novak, MSc Michal Amit, PhD Department of Obstetrics and Gynecology Rambam Health...

Báo cáo sinh học: " Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" potx

Danh mục: Hóa học - Dầu khí

... MSC get through the blood brain barrier (BBB) after stroke At first, one of potential mechanisms is passive translocation of MSCs to the brain parenchyma through a disrupted BBB after stoke The ... hour, and were concubated overnight at a dilution of 1:200 with FITC labeled goat anti-GFP (AbCam) and rabbit anti-rats Neuronal nuclei (NeuN, which is a marker of neuron.) (DAKO), and then incubated ... homogeneous population of fibroblast-like cells, (B) The Passage MSCs grew as whorls of densely packed spindle-shaped (scale bar = 200 um in A and B) (C) Adipocyte differentiation of MSCs: Upon induction...

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adhesion and osteogenic differentiation of human mesenchymal stem cells supported by b type carbonated hydroxylapatite