... mutant E1 In all assays, the E1aS283C and E1aF26 6A mutants behaved essentially the same as wild-type E1 In the DCPIP assay, the E1aY28 1A and E1aR28 2A mutants displayed a catalytic activity about ... Kinetic parameters of wild-type and mutant E1s determined by the DCPIP assay Pyruvate ThDP kcat (s)1) Wild-type E1aF26 6A E1aR26 7A E1aD27 6A E1aY28 1A E1aR28 2A E1aS283C E1aY28 1A/ R28 2A/ S28 3A E1aY28 1A/ R28 2A ... activity about twice that of wild-type E1 The single mutants E1aR26 7A and E1aD27 6A and the multiple mutants E1aY28 1A/ R28 2A/ S28 3A and E1aY281S/R282S displayed catalytic activities 2.5 and 3.5 times, respectively,...
... green) and TAA (in black) The superimpositioning was guided by the catalytic acids (D179AMY2, E204AMY2, and D289AMY2 and D206TAA, E230TAA, and D297TAA) The invariant Y51AMY2 and Y82TAA are at subsite ... substrate analogues derived from acarbose and barley a- amylase (AMY2 [16]); and Taka-amylase A (TAA [17]) (A) Stereo view of interactions involving segments of ba loops and (i.e domain B) from AMY2 ... Bacterium Bacterium Bacterium Bacterium Yeast Bacterium Archaea Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Bacterium Plant Plant a- Amylase Barley...
... appears as a row, y as a column, and z as an entry A k-plex is a subset of L in which each symbol appears as a row, column, and entry precisely k times A 1-plex is called a transversal and a k-plex ... then a1 (a2 (· · · (ak ) · · · ) ∈ A( Q) Proof Set A := A( Q) (i) Let ρ = La1 · · · Lak Then A (qA) = a1 (a2 · · · (ak q) · · · )A Since Q /A is a group, we may reassociate to get A (qA) = a1 (a2 ... the translation notation feels cumbersome, the idea is very basic Given the product a1 (a2 (· · · (ak x) · · · ) = x, we may reduce both sides mod A to get a1 Aa2 A · · · ak AxA = xA a1 Aa2 A ·...
... measurements and involving all stages of disease and a demographically broad sample Prospective rather than cross-sectional measurements have important advantages, in guarding against cohort bias (differences ... patient group in a rheumatology practice? The available literatures have been based on a posteriori analyses of data from randomized, controlled trials and small, qualitative research Figure arthritis ... patients with an HAQ-DI greater than a threshold value can serve as a standardized 'morbidity ratio' of that particular patient population Using this method, Sokka and colleagues compared the HAQ-DIs...
... mediating/causal factors in the association between the mortality rates and the MGI Either way, in all multivariate models, the association between globalization and the mortality indicators remains ... associations between the indicators used For this we applied the non-parametric Spearman’s correlation analyses, as not all variables showed a normal distribution [37]iii Martens et al Globalization ... correlations, and simple and multiple linear regression analyses) indicate that the infant morality rate, under-five mortality rate and adult mortality rate all show a negative association with...
... Science, and Technology, and the Ministry of Health, Labour and Welfare, Japan 19 References 20 10 11 Shibata R, Sakai H, Kawamura M, Tokunaga K, Adachi A: Early replication block of human immunodeficiency ... interests 14 Authors' contributions TS and EEN designed the research, AK, AS, YS, and EEN performed the research, TS, MN, AA, and EEN analyzed the data, and AA, HA, TS, and EEN wrote the paper 15 Acknowledgements ... neutralizing antibodies Nat Med 2000, 6:207-210 Nishimura Y, Igarashi T, Haigwood N, Sadjadpour R, Plishka RJ, Buckler-White A, Shibata R, Martin MA: Determination of a statistically valid neutralization...
... considered Alternative approach I: linear regime One such alternative approach is applicable when the relationship between the membrane voltage and the calcium influx and the indicator signal is linear ... disadvantage is that enough dual measurements of membrane voltage and indicator need to be at hand to calculate the optimal reverse filter grev(t) As another caveat, this method only works as ... numerical simulations of calcium dynamics, analytical treatment of the resulting equations are beyond the scope of the present paper and have to await future investigation Feasibility of the approach...
... Intensive Care Med 2004, 30:996-997 doi:10.1186/cc8189 Cite this article as: Aardema LM, et al.: Value and price of ventilator-associated pneumonia surveillance as a quality indicator Critical Care ... in the acute care setting Am J Infect Control 2008, 36:309-332 Tulleken JE, Zijlstra JG, Ligtenberg JJ, Spanjersberg R, Van der Werf TS: Ventilator-associated pneumonia: caveats for benchmarking ... for the interdisciplinary team Crit Care Med 2006, 34:211-218 Horan TC, Andrus M, Dudeck MA: CDC/NHSN surveillance definition of health care-associated infection and criteria for specific types...
... of data that could allow for a structured evaluation of the organizational changes It is evident that a well-planned evaluation of changes in the organizations, before they are actually made, ... pre-hospital deaths and priority A assignments was quite small, and was spread over several years Another bias in our result could have been caused by the impact of external factors such as ambulance ... on performance Acta Anaesthesiol Scand 2010, 54(6):689-695 Handbooks of the Ministry of Social Affairs and Health Ambulance and emergency care services: A handbook for drawing up an alarm procedure...
... exists Zaidi A, Clough P, Cooper P, Scheepers B, Fitzpatrick AP Misdiagnosis of epilepsy: many seizure-like attacks have a cardiovascular cause J Am Coll Cardiol 2000;36(1):181-4 Kanjwal K, Kanjwal ... 210 diagnosed as epilepsy (1, 2, 3) An ILR can be invaluable in identifying these patients and facilitating prompt therapy (3) Conflict of Interest References The authors have declared that no ... Clinical Symptoms Associated with asystolic or bradycardic responses on implantable loop recorder monitoring in patients with recurrent syncope Int J Med Sci 2009; 6:106-110 Kanjwal K, Karabin...
... Step 2: Addinga swap file To add swap space on a system, add a swap file by using the following steps: Create a file that is equal in size to the swap file that is going to be created Do this ... mkswap –f /root/swap.swp or type /sbin/mkswap –f /root/swap.swp To activate this swap file, use the swapon command To make this swap file inactive, use the swapoff command in place of the swapon ... in made in /etc/fstab Reflection This method is the quickest and easiest way to add swap space However, it does create some problems By creating this swap file on a partition that is already...
... DisplayDataRow( DataRow myDataRow, DataTable myDataTable ) { Console.WriteLine("\nIn DisplayDataRow()"); foreach (DataColumn myDataColumn in myDataTable.Columns) { Console.WriteLine(myDataColumn ... mySqlConnection.CreateCommand(); mySqlCommand.CommandText = "SELECT CustomerID, CompanyName, Country " + "FROM Customers"; SqlDataAdapter mySqlDataAdapter = new SqlDataAdapter(); mySqlDataAdapter.SelectCommand ... underlying DataTable until you call the AcceptChanges() method of your DataTable For example: customersDT.AcceptChanges(); Note You can call the RejectChanges() method of a DataTable to undo...
... // Add the tables to the DataSet and create the relationship ds = new DataSet( ); ds.Tables.Add(parentTable); ds.Tables.Add(childTable); ds.Relations.Add(new DataRelation("Parent_Child_Relation", ... System.EventArgs e) { DataColumnCollection cols; DataColumn col; // Build the parent table DataTable parentTable = new DataTable(PARENTTABLENAME); cols = parentTable.Columns; col = cols.Add(PARENTID_FIELD, ... generate new GUIDs for primary keys parentTable.RowChanging += new DataRowChangeEventHandler(parentTable_RowChanging); childTable.RowChanging += new DataRowChangeEventHandler(childTable_RowChanging);...
... create a table in the database and a primary key on the new table in a SQL Server database You can programmatically drop a table using the DROP TABLE statement in a similar way To drop the table ... syntax for each database varies slightly because of differences in database server capabilities and architecture For example, the CREATE TABLE statement for Oracle is different because Oracle ... Management Language (DML) Used to manipulate—select, insert, update, and delete—data in the database objects Database objects are defined using DDL The solution executes a DDL CREATE TABLE statement...
... (cathepsin C): exclusion domain added to an endopeptidase framework creates the machine for activation of granular serine proteases EMBO J 20, 6570–6582 18 Molgaard A, Arnau J, Lauritzen C, Larsen ... N-terminal trunk binds into the nonprimed Table Average distances between CA atoms of the stefins and catalytic residues of cysteine proteases Distance calculated ˚ d (A) Papain–stefin B Cathepsin ... Karaoglanovic-Carmona A, Juliano L & Turk D (2000) Crystal structure of cathepsin X: a flip-flop of the ring of His23 allows carboxy-monopeptidase and carboxy-dipeptidase activity of the protease...
... cigars manufactured by the company are marketed under various international, regional, local and niche trademarks, but all carry the “Habanos” label This name has been registered as a geographical ... agreements that are directly related to authenticity and tradition, and the more regulatory or conventional agreements that guarantee safety and facilitate product marketing (Casabianca et al 2000) ... industrial products can aspire to a geographical indication, whereas, in EU states, only agricultural and agri-food products can achieve recognition (Wattanapruttipaisan 2009) The TRIPS Agreement also...
... markets Market performance Differences in market practices Commercial real estate sectors The US real estate market Market performance Commercial real estate sectors The Asia Pacific real estate market ... real estate? Gaining exposure to real estate Real estate derivatives Key benefits and challenges of investing in real estate Global real estate investment The UK and European real estate markets ... business areas are Alternative and Quantitative Investments, Global Real Estate, Infrastructure, Private Equity and Fund Services UBS Global Asset Management combines investment expertise and attention...
... Ottawa, 2000, page 3 Health Canada, Health Canada’s Gender-based Analysis Policy, Ottawa, 2000, page 4 Health Canada, Health Canada’s Women’s Health Strategy, March 1999, page Health Canada, Health ... blanket health warnings about smoking Health Canada, Health Canada’s Gender-based Analysis Policy, Ottawa, 2000, page Health Canada, Health Canada’s Gender-based Analysis Policy, Ottawa, 2000, pages ... Canada, Access to Health Care Services in Canada 2001, catalogue no 82-575-XIE, June, 2002, and Statistics Canada, CANSIM II database 15 Health Canada, A Report on Mental Illnesses in Canada,...
... substrate (SIGMA 104Ò, Sigma-Aldrich, Bornem, Belgium) solution was added and the absorbance was measured after 30 and 60 at 405 nm on a SPECTRAmax PLUS384 microplate reader system (Molecular Devices, ... increasing pH OD was measured 60 after adding the substrate (B) Inhibition ELISA with monosubstituted HNE peptides Each Cys was replaced by an Ala (C38 1A, C38 6A, C39 4A) or an amino butyric acid ... displayed quasi-identical backbone structures and side-chain orientations (Fig 7A, B) The circular peptide appears as a fairly flat structure with an amphiphilic character The hydrophilic amino acids...