... Anticoagulant activityof ticks 50 3.1.1 Protein quantification ofcrudesalivaryglandextracts50 3.1.2 Activityofcrudesalivaryglandextracts50 3.1.3 Purification ofsalivaryglandextracts ... Anticoagulant activityofcrude R pulchellus salivaryglandextracts 51 Figure 3.2 Anticoagulant profile of R pulchellus SGEs 52 Figure 3.3 Anticoagulant profile of D reticulatus ... 2.1 Salivary glands and extractsSalivarygland and salivaryglandextractsof ticks were obtained from collaborators RNDr Mirko Slovak and RNDr Maria Kazimorova from the Slovak Academy of Sciences,...
... analysis of a T infestans salivarygland extract and puried recombinant triafestin-1 and triafestin-2 A crudesalivarygland extract (lane 1), lg of recombinant triafestin-1 (lane 2) and lg of recombinant ... and expression of recombinant triafestin-1 and triafestin-2 A cDNA library was constructed from the salivary glands of unfed fth instar nymphs of T infestans cDNA fragments from 550 clones were ... activation of FXII and PK FXII was preincubated with various concentrations of triafestin-1 or triafestin-2 for 10 The activation reaction was started by addition of PK and DS 500 The total activity of...
... of T infestans saliva Fig Western blot analysis of triplatin-1 and -2 from the salivaryglandof T infestans Extracts from a pair ofsalivary glands, recombinant triplatin-1 and recombinant triplatin-2 ... Isolation and sequencing of cDNA clones Salivary glands of T infestans were dissected from thoraces of unfed adults, and poly A(+) RNA was isolated from 30 pairs ofsalivary glands using a MicroPrep ... Biochemistry 67, 143– 150 Keller PM, Schultz LD, Condra C, Karczewski J & Connolly TM (1992) An inhibitor of collagen-stimulated platelet activation from the salivary glands of the Haementetia of cinalis...
... homozygous double congenic strain The purpose of the present study was to monitor the influence of each of these two loci on the incidence and severity of sialadenitis, and to identify genes located ... samples included high amounts (from to μg/μl) of good quality RNA Salivarygland gene expression The total RNA was submitted to the RNA-in/data-out service of the Norwegian Microarray Consortium Core ... significance level of 0.05 For each assay, one reaction without Multiscribe RT enzyme was included, to ensure RNA specificity of the assays Results Influence of NOD Idd5 and Nss1 loci on severity of sialadenitis...
... Diseases of the salivary glands Inflammatory salivarygland diseases, next to benign neoplasms, are the most frequent causes ofsalivarygland swelling in juvenile age [3] The acute forms of sialadenitis ... to Eneroth [13] salivarygland tumors make up 0.3% of all human tumors, and less than 10% of all juvenile head and neck tumors are located in the salivary glands [14] Only 1% of all head and ... instance of persisting postoperative nerve injury or symptomatic Frey’s syndrome following total extirpation of the parotid gland due to chronic sialadenitis Page of Tumors of the salivary glands...
... differentiation of secretory acinus and related ducts, the functional unit ofsalivary glands Immunohistochemical studies demonstrated different degrees of EGFR expression in several salivarygland carcinomas, ... the antitumor activityof anti-EGFR targeted agents in the salivarygland neoplasms In a phase II study of cetuximab monotherapy in 22 patients with recurrent/metastatic salivarygland carcinomas, ... palliative management of advanced salivarygland cancers J Clin Oncol 2006, 24:2673-2678 Agulnik M, Siu LL: An update on the systemic therapy of malignant salivarygland cancers: role of chemotherapy...
... the presence of proteins able to bind to the four DENV serotypes in mosquito salivaryglandextracts Because SGE might contain both salivarygland tissue (basal lamina or salivarygland epithelial ... phenylmethylsulfonyl fluoride and 10 μg/ ml of aprotinine) Each vial contained about 1 ,500 pairs ofsalivary glands and was stored at -80°C until needed [8] Salivary glands were then thawed and disrupted ... monoclonal polynesiensis salivary glands DENV-binding proteins from Ae polynesiensis salivary glands detected with anti-E monoclonal antibodies Ae polynesiensis salivaryglandextracts were treated as...
... currently professor of nutritional biochemistry in the Department of Nutrition and Dietetics, King’s College London and honorary professor of clinical biochemistry in the Department of Clinical ... disruption of mitochondrial membrane potential, activation of multiple caspases, arrest of cell cycle progression, and induction of differentiation The positive effect of this compound on other types of ... survival The ability of SFN and PEITC to inhibit the transcriptional activityof NF-κB is a consequence of the phytochemicals antagonizing phosphorylation of IκB, the inhibitor of NF-κB, which is...
... found in the salivary glands To detect protrialysin stored in the salivary glands, and determine whether it would retain lytic activity, glands were extracted in the presence, or absence, of APMSF, ... M Martins et al using twofold dilutions ofsalivary glands extracts, or stock solutions of the peptide proP7 Recombinant protein expression and purification An NL region of preprotrialysin (spanning ... amounts ofsalivarygland homogenates prepared in the presence (d) or absence (s) of APMSF In (A) the number of surviving cells was determined in a hemocytometer, and the percentage of lysis...
... the salivary glands of patients with SS There is clear evidence of an inflammatory environment with presentation of self-antigens characteristic of SS [22] that may permit the production of autoantibodies ... the CDR of VH and VL gene rearrangements of B cells obtained by minor salivarygland biopsies from two patients with SS [6] Detailed analyses of the frequency of occurrence and mutations of the ... each codon of productively rearranged VL genes of B cells isolated from peripheral blood and from the parotid glandof a patient with Sjögren’s syndrome The frequency of mutation of each codon...
... pathogenesis of RA after fractalkine and CX3CR1 expression were reported to be upregulated in the synovium of patients with RA [9] Supporting the notion of the disease-modulating activityof fractalkine ... Pichardo EL, Shah N, Peck AB, Humphreys-Beher MG: Elevated levels of cysteine protease activity in saliva and salivary glands of the nonobese diabetic (NOD) mouse model for Sjögren syndrome Proc ... profile but not with histopathologic changes in the salivarygland Arthritis Rheum 2006, 54:23002305 Delaleu N, Immervoll H, Cornelius J, Jonsson R: Biomarker profiles in serum and saliva of...
... negative in primary adenocarcinomas of the lung, a salivarygland origin was most likely Immunohistochemistry might also aid in the differential diagnosis ofsalivarygland carcinoma types In the present ... incidence of the adenocarcinoma (NOS) is decreasing with the establishment of new neoplastic entities of the salivary glands, this carcinoma still occurs and should be taken into account in case of ... Adenocarcinoma ofsalivary origin: Clinicopathologic study of 204 patients Am J Surg 1982, 144:423-31 Matsuba HM, Mauney M, Simpson JR, Thawley SE, Pikul FJ: Adenocarcinomas of major and minor salivary gland...
... Myoepithelioma is a rare benign tumor of the salivary glands and is usually seen in the parotid gland and the minor salivary glands It was once considered to be a type of pleomorphic adenoma (PA), but ... tumor is usually located in the parotid gland and the minor salivary glands of the soft palate and represents less than 1% of all salivarygland tumors [2] Several authors now consider this tumor ... epithelial neoplasms In Tumors ofSalivary Glands AFIP; 2002: 57-68 Ferri E, Pavon I, Armato E, Cavaleri S, Capuzzo P, Ianniello F: Myoepithelioma of a minor salivaryglandof the cheek: case report...
... with tumor-like lesions of the salivary glands at the outpatient care of an ENT-department (university hospital) 30.7% of the patients with tumor-like lesions in salivary glands underwent an imaging ... sensitivity of 98%, a specificity of 52% and an accuracy of 84% According to Takashima et al., MRI achieved a sensitivity of 60%, a specificity of 88% and an accuracy of 81% in the assessment of tumor ... Adenoma of the left parotid gland Pleomorphic Adenoma of the left parotid gland: Ultrasound image axial and Ultrasound image transversal ultrasound head or located deeper in the salivary gland, ...
... 1C) The effect of the length of the incubation period on the rate of inhibition of the HIV-1 infection of the CEM Table Mucin toxicity Sample Con CEM SS cells % of dead cells % of live cells MUC5B ... of the salivary glands which results in a decline in the amount of saliva and a change in its constituents [10] This in turn may affect the glycosylation pattern or sugar composition of the salivary ... immunoreactivity differences between salivary MUC5B and MUC7 mucins Habte et al Virology Journal 2010, 7:269 http://www.virologyj.com/content/7/1/269 Page of Figure Anti-HIV-1 activities of salivary...
... to estimate activityof the RAS with a greater decrease in SBP connoting augmented system activity [19] Serum ACE Activity: ACE refers to the angiotensin converting enzyme Serum ACE activity was ... dose of 10 mg/kg Each rat received a single dose of LNAME Measurements of SBP were taken at four, seven, 10, 15, and 20 minutes post injection The area under the curve was used to estimate activity ... initiated on day 338 in the life of the female SD, the SBP of all 40 SD ingesting the control diet containing 20% sucrose had been recorded previously starting on day 50of life up to that point in...
... antioxidant extracts, each of which has been claimed to exert chemopreventive activity in cellular models of cancer [43,44] Recent publications have stated that the antiproliferative activityof flavonoids ... Polyphenolic fractions at IC50 Polyphenolic fractions at IC50 Fig Scavenging activityof OH and OÁÀ analyzed by ESR Grape and pine fractions were evaluated at: (A) 50 lM and (B) IC50 in HT29 cells in ... properties of natural extracts D Lizarraga et al Table Comparative chemical characteristics and HT29 cell growth inhibition of grape and pine fractions Percentage of galloylation (%G), mean degree of...
... respectively, in isoform A of SAL, the structure of which is described here, and are replaced in isoform B by Ala and Val, respectively Replacement of residues 43 and 75 with those of isoform B in the ... OBP of 441 A3, hamster ˚ OBP of 500 A ˚ aphrodisin 350 A3, and horse allergen (Fig 5B), has the ˚ smallest, 273 A3 The small size of the main cavity in SAL may be due to the presence of ... periphery of the protein (Fig 5A) Unexpectedly, the volume of the main cavity is one of the smallest among lipocalins of known three-dimensional structures The ˚ cavity of MUP has a volume of 510...
... anticoagulants of H longicornis It was previously reported that anticoagulants are present in the salivary glands of H longicornis [24] According to this report, an extract of the salivary glands prolonged ... Chemical Industry, Osaka, Japan Mass sequence analysis of cDNA clones H longicornis salivarygland mRNA was isolated from 10 pairs of glands of ticks at three distinct feeding stages (unfed, slow ... mechanism of action of human antithrombin-heparin cofactor J Biol Chem 248, 6490– 6505 14 Tollefsen, D.M., Pestka, C.A & Monafo, W.J (1982) Heparin cofactor II Purification and properties of a heparin-dependent...
... concentrations, solution of50 ll was pipetted into the 96 orifice plate and followed by addition of 150 ll DPPH reagent ( 250 lL DPPH per liter methanol) After 30 min, the absorbance of the mixture at ... the conversion of the Au(III), X the value of any of the five parameters of each broth, Cov(X, Y) the covariance value and rxy the correlation coefficient of X and Y with a range of [-1, 1], E the ... Technologies Holdings Co Ltd.) Mixture of ethanol solution ( 150 ll) and the broth (50 ll) served as the blank and DPPH solution ( 150 ll) plus ethanol (50 ll) as the control The DPPH radical scavenging...