... at the two spatial tips ofthe molecule, at the N-terminus with Arg1 and in the turn in the presence of Arg7 and Arg9 (see Figs and 4) The shorter analogue, BM-2, was the most flexible of all the ... ofthe peptide Two hydrophilic regions were located at the two spatial tips ofthe molecule, at both termini with Arg1 and Arg17, and in the turn in the presence of Arg8 and Arg10 (see Figs and ... on the membrane This may also account for differences in the haemolytic activity ofthe peptides A better understanding ofthe mode of action of these peptides is crucial for the development of...
... in the ÔupÕ position rows and shows that in the presence ofthe substrate, rotation of Asp142 towards Glu144 lowers the pKa ofthe latter by 0.8 pH units The calculated effects ofthe D140N and ... for the position of residue 142 [34] The pKa calculations indicate that the only candidate for a residue determining the basic arm ofthe pH-activity profile ofthe D142N mutant is Glu144 The ... simplicity, and in line with the results from the other calculations, the 20.0 to Asp142 b The calculations were performed on the crystal structure ofthe Dl40N...
... bound to the s-site only, and inactive mR14b, with d-ATP bound to the s- and a-sites [2] Addition of l-ATP should lead to saturation ofthe a-site and complete conversion to mR14b, leading to the ... higher than the apparent Km for d-ADP of 91 lm (Fig 6A,B) As above, this probably reflects retention ofthe base and especially b-phosphate interactions at theactive site and loss ofthe specific ... binds first to the s-site, inducing active R12 formation, and then to the a-site, inducing inactive R14b formation, thus accounting for the biphasic curves observed The changes in the curves in...
... Stereo figures ofthe model ofthe bound substrate in the AD2 E526A mutant (A) and D524A mutant (B) The structures of bound sugars andthe side-chains of three acidic residues in the conserved ... conformational changes, but ofthe removal of negative charge at these residues Conformation of chito-oligosaccharides bound to theactive site cleft For the structural determination ofthe AD2–substrate ... D524A, E526Q and E526A) The D522N and D522A mutants retained about 40% and 20%, respectively, ofthe wild-type kcat values The D524N mutation increased the Km value slightly, and decreased the kcat...
... recorded in the spectra were mapped onto the anticipated sequence of subunit C on the basis of their mass value andthe specificity ofthe enzyme, leading to the complete verification ofthe amino ... trifluoroacetic acid The identification ofthe flavin cofactor was obtained by comparing the retention time ofthe eluted peak with that of reference samples of authentic FAD and FMN Characterization of recombinant ... soluble fraction ofthe induced cell in the case ofthe expression of pET22b(+)/touBEA and /touC, whereas the product ofthe expression of pET22b(+)/touF was accumulated in the insoluble fraction,...
... is the angle between the projection ofthe tilt ofthe z axis on the xÂ, y plane (dened direction of tilt of z andthe x axis), and j % a + c denes the location ofthe rhombic (x, y) axes The ... The two structures of WT metHb and rWT metHbH2O, exhibit signicant differences in the interaction ofthe FG loop with the heme and in the position ofthe B-helix, with Tyr32(B10) closer to the ... of WT andthe other of rWT metHb, exhibit differences that include important portions ofthe protein that we have characterized above Thus parts ofthe FG corner move away from the heme and the...
... in the design ofthe study and performed the statistical analysis ML treated some ofthe patients included in the study and participated in the critical discussion ofthe data AM helped draft the ... to the combined sparing ofthe contralateral parotid and part ofthe contralateral submandibular gland Certainly, the salivary function can be further preserved using ipsilateral IMRT because the ... because ofthe morbidity of radiotherapy The results ofthe present study demonstrate that the failure rate in the opposite neck is rare in selected cases with well lateralized tumors ofthe tonsillar...
... stimuli [11-13] However, in spite ofthe relevance of these reservoir cells in the control of viral persistence, the mechanisms responsible of apoptosis resistance of persistently-infected cells ... independent ofthe magnitude of viral replication Apoptosis resistance is independent ofthe magnitude of viral replication A) Jurkat and J1.1 cells were incubated in the presence or absence of 1000 ... experiments of apoptosis by Hoechst and APOBrDU DOC and GAR were responsible of experiments using Western blot analysis, and contributed to writing ofthe manuscript MB, RL and MS performed and interpreted...
... 10 My pocket has been picked (by someone) 11 The enemy has been defeated by our army 12 I shall be obliged to go (by circumstances) Stay on top of your writing! Download our grammar guide from...
... mentioned The men, especially the husband andthe grandfather, sip alcohol and tell their family members what they see and hear or remind them of right or wrong things in life The others also ... denoting the objects covered by the scope ofthe notion expressed in the word, andthe significative function is the function of expressing the content ofthe respective notion The function of rendering ... difficult The oldest or the husband ofthe family sits at the head ofthe table and others sit in descending order according to age or wherever they like The meal starts when everyone has their own...
... “Introduction” states the rationale ofthe study, Semantics can be simply defined as the study of meaning It defines the aims, objectives andthe significance, presents the is central to the study of communication ... review ofthe research Chapter 3: “Methodology and Procedures” mentions the method and procedures ofthe research Chapter 4: “Findings and Discussions” is the main frame of need to understand semantics ... element ofthe meaning of a word; a description ofthe characteristic feature of what the word refers to E.g the sense of “grasshopper” is “an insect which can jump high the study, which describes and...
... recognition ofthe field Andthe findings of semantic aspects often borrow the WDBs for not only conveying the denotative as well as analysis of pragmatic aspects of these kinds of words will ... understand their cases of Secondly, English considers the WDB “owl” as the symbol meaning, it is important to consider them in their collocations From of “wisdom” on the basis of their experience and ... pragmatic features ofthe WDBs The finding ofthe pragmatic features ofthe WDBs leads both teachers and learners of study may be in one way or another beneficial to the language English and Vietnamese...
... disappearance ofthe heavier inactive peak Furthermore, FPLC analysis ofthe recombinant wild-type cN-II showed the presence ofthe tetrameric active form irrespective ofthe presence of effectors ... addition to the tetramer However, stabilization ofthe dimeric form had no effect on the catalytic capacity ofthe enzyme Our results show that the monomer is probably inactive, andthe dimer is the ... determine the value of K50 for these compounds Under these experimental conditions, the accumulation of radiolabelled inosine (nucleotidase activity) represents the sum ofthe phosphatase andthe phosphotransferase...
... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH ofthe ribose is hydrogen bonded to the main chain andthe side chain of Asp142 (Fig 1C) Both hydroxyl groups ofthe ribose are involved in ... Asp142, 2¢ and 3¢OH ofthe ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate ofthe GMP is hydrogen bonded to main chain and side chain atoms of amino...
... position at the present day The present theories ofthe transmission of light and sound; ofthe production of winds, and sun-spots, andofthe method of development and dissemination of heat, are ... except in the direction of other spheres These theories throw new light upon the character and extent ofthe atmosphere ofthe moon and planets, andthe consequent availability of those and other ... intimate and inherent nature of those forces? Do they, or either of them, belong to the domain ofthe supernatural? Are they the products of some supreme force, or forces, heretofore unappreciated? The...
... regards as the equivalent of thc npper part of t h e Portland Beds of southern England If, with ARKELL (1946, pp 24-28), we place the Portland Beds in the upper part ofthe Lower Volgian, they, the ... whorls of Tollia pnyeri Bitween 360 and 370 m, on the same flank ofthe moontain, the writer found similar doggers and platy-weathering sandstones, which contained occasional exarnples of Surifes and ... but further than this I am not prepared to go The dip ofthe rocks exposed in the 17all~gsides is often vcry nearly 1v Stratigraphy and Anr~noniteFauna of East Greenland 13 the same as the gradient...
... cause of offence In these habits we resemble the Aborigines of our country They load their huge pipes with the dried leaves of this plant, and when lighted, they breathe the dark cloud of smoke ... men void of understanding; capable of soothing the sorrows ofthe afflicted, of healing the wounds ofthe spirit, and assuaging the anguish of a broken heart But how it fulfils these desirable indications, ... form or other, either to ease the pains of dyspepsia, to allay the horrors of tedium vitæ, or to drown the anguish of a guilty conscience And may not many of these patients say to those ofthe Faculty,...