activated cd4 cd25 t cells via tgf b
Báo cáo y học: " HTLV-1 HBZ cooperates with JunD to enhance transcription of the human telomerase reverse " pptx
... investigated whether HBZ, in association with c-Jun or JunD, is able to regulate the activity of the hTERT promoter We demonstrated that HBZ together with JunD synergistically activates hTERT http://www.retrovirology.com/content/4/1/92 ... insight by which hTERT transcription is up-regulated by this viral protein Results HBZ regulates the activity of the hTERT promoter To examine the role of HBZ in regulating the activity of the hTERT ... increases the hTERT promoter activity, but to an extent lower than that observed in HeLa cells < /b> Both the N-terminal and leucine-zipper regions of HBZ are required to increase the hTERT promoter activity...
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Báo cáo y học: "Use of chinese and western over-the-counter medications in Hong Kong"
... pharmacists, we contend that the role of community pharmacists in primary care must not be underestimated This is vividly illustrated by the fact that 9.4% of the respondents had no consultation with ... characteristics were described by cross tabulations of the three patterns (OTC use only, sought consultations only or both) with other demographic, socioeconomic and health related variables Chi ... Report - Report No 30, Census and Statistics Department, The Hong Kong SAR Government [http://www censtatd.gov.hk/products_and_services/products/publications/ statistical_report/social_data/index_cd _B1 130230_dt_latest.jsp]...
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Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"
... out to be refractory against TMZ as shown in the TMZ-based therapy of advanced CaP In contrast to the described DNA fragmentation of glioma cells < /b> after TMZ treatment [54] the TMZ-BioShuttle treated ... intact cells < /b> with intact DNA content could be observed with higher relative fluorescence intensity in the gate M2 The observation of effects of the TMZ molecule and its TMZ-BioShuttle respectively ... increased but constant on the untreated lymphocytes and in the TMZ-BioShuttle at 10.2 % whereas the TMZ-probe showed 14.2 % dead cells < /b> It turned out that high-resolution flow cytometric measurements...
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Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
... product inhibitor, and it seems likely that this mechanism contributes to the reported 5¢-AMP activation of NR and GS Both the NADH pyrophosphatase activity and the inhibition of NR were inhibited ... 10 at 30 °C by an amount of a Mono Q fraction that appeared to inhibit NR by 55% (Table 3) These findings suggest that the NR/GS inhibitor functions during both the assay preparation and the assays ... purified further (Fig and Table 1) Similar to the interfering protein, the GS inhibitor was eluted from Q-Sepharose by 0.2 M NaCl (Table and data not shown) Consistent with the possibility that the NR...
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Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx
... we did not observe AMPK suggesting that there may be no physical interaction between the two proteins (data not shown) Taken together, this indicates that MCD may not be a direct substrate for ... be a direct substrate for AMPK [12,25] However, it cannot be excluded that AMPK could indirectly modulate MCD activity in intact cells < /b> Stimulation of MCD activity with AICAR in intact cells < /b> was ... FEBS 2004 2832 N Sambandam et al (Eur J Biochem 271) that MCD can either be inhibited or activated < /b> by phosphorylation [16,17] One potential kinase that could control MCD activity is 5¢AMP-activated...
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Company Insights on BP, Microsoft, and Western Digital
... illegal The Clayton Antitrust Act forbade mergers between competitors where the impact of a merger would be to substantially lessen competition The Federal Trade Commission Act created the Federal Trade ... of twenty states sued Microsoft, accusing the company of stifling both Internet browser competition and consumer selection to extend its operating system dominance Perhaps the greatest footnote ... promote and maintain competition in an industry The Sherman Antitrust Act outlawed agreements to fix prices, limit output, or share the market and declared that monopolies and attempts to monopolize...
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Illustrated Dictionary Of Symbols In Eastern And Western Art
... antelope See also DEER; GAZELLE Antlers A hunter confronted by a stag with a crucifix between its antlers is St Eustace or St Hubert [v: Italian, 18th cent.] It is also their attribute The cult ... deity and was often the consort of the MotherGoddess Its identification with known deities begins with the Hittite storm-god, Teshub, who holds a lightning bolt and stands above a bull (c 1000 BC) ... large numbers between the 6th and 12th cents., an artistic tradition that has continued to the present day The term is also used of Japanese art to denote a devotional image of a Buddhist deity surrounded...
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The Clinical Practice of Complementary, Alternative, and Western Medicine pot
... instances the attractor is so strong that it prohibits movement to any other state, the biology is then stuck, and its plasticity and its reactive abilities are compromised The attractor seems to represent ... in treatment of his patient, provided that the practitioner can demonstrate adequate training in the modality, that the patient was informed, and that the treatment was not grossly inappropriate ... wine These thoughts, to the uninitiated, will have the quality of Kool-Aid, at best, refreshing but without much substance To take the quantum leap from the comfort of the known deterministic...
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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
... to +1) sites driving the CAT reporter gene in U937, but not in CHP100, cells < /b> showed that the )107 construct was up-regulated by leptin (Fig 4B, wt and wt + L) Disruption of this site by mutation ... (Fig 5B) Taken together, it can be concluded that in human U937 cells < /b> leptin by binding to LR activates STAT3, which in turn up-regulates FAAH gene transcription by tethering to a CRE-like site ... heatinactivated fetal bovine serum was added at a final concentration of 10%, as reported [15] Controls were incubated with vehicles alone Cell viability after each treatment was tested by Trypan...
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PHƯƠNG PHÁP WESTERN BLOT & ELISA
... Buffer Protease & phosphatase Centrifuge tube VOATEX Mẫu chứa protein collect the supernatant LY T< /b> M 40C 40C ultra speed centraifuge tube RIPA Lysis Buffer: Lysates t< /b> b o Tris-HCl ... I.Giới thiệu Western blot Dùng để ph t < /b> protein chuyên bi t < /b> hỗn hợp phức t< /b> p nhiều protein(mô,dịch chi t < /b> mô) Western blot ph t < /b> triển George Stark Công nghệ dùng kháng thể để xác định vị trí Protein ... Chuẩn b mô thường thực nhi t < /b> độ lạnh để tránh biến t< /b> nh protein suy thoái ,b o vệ mẫu K t < /b> hợp voatex,ly t< /b> m Nhanh chóng giúp ly giải t< /b> b o hòa tan protein Video b ớc chuẩn b mẫu SDS -T< /b> c nhân...
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báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
... determined by real-time quantitative PCR (data not shown) This indicates that the integration levels are similar with both constructions but that, as previously described, the promoter activity is ... not modified compare to non-transduced cells < /b> (data not shown) Our results indi- cate that the LV transduction does not alter the phenotype of pDC or their capacity to mature Functional properties ... Authors' contributions VP contributed to the experimental design, data acquisition and analysis, and drafting of the manuscript BS contributed to the data acquisition and analysis MS designed lentiviral...
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báo cáo hóa học:" Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function" ppt
... results suggest that activation-induced expression of Foxp3 in CD4+< /b> CD25+< /b> T < /b> cells < /b> is more stable than that in http://www.translational-medicine.com/content/7/1/89 CD8 +CD25+< /b> T < /b> cells < /b> Absolute number ... heat inactivated FBS Allogeneic stimulating cells < /b> were irradiated in a cesium irradiator to a total dose of 5,000 rad, to abolish their capacity to proliferate Cultures were set up in flat-bottomed ... analyzed for the detection of CD25+< /b> Foxp3+ cells < /b> (A; bottom panel) Gated CD4+< /b> T < /b> cells < /b> (B; top panel) or CD8+ T < /b> cells < /b> (B; bottom panel) were analyzed for the expression of CD44< /b> , CD62L, Foxp3 The CD44< /b> +...
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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
... congenital or acquired defects Other objectives would be to establish the culture conditions of pluripotent stem cells < /b> using reliable cytotoxicity tests and the optimum type of cell or tissue to be ... related to identification of therapeutic targets and potential therapeutic tests Within these general objectives, other specific objectives will be related to studies of cell differentiation ... reliable cytotoxicity tests and the optimum type of cell or tissue to be transplanted depending on the disease to be treated Up to now, most cell therapy protocols have not been controversial The...
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Báo cáo sinh học: "Engineered artificial antigen presenting cells facilitate direct and efficient expansion of tumor infiltrating lymphocytes" pot
... autologous TILs CD3/28 beads not support TIL expansion (3:1 bead to T < /b> cell ratio) Day cell counts are shown (d) TILs stimulated with KT64/BBL aAPCs with or without anti-CD28 antibody not secrete IL-2 ... suboptimal and similar in level to that observed after activation with KT64/BBL aAPCs loaded anti-CD3 mAb at the 0.5:1 aAPC to T < /b> cell ratio By comparison, all PBLs divided extensively after stimulation ... CD3+ T < /b> cell contribution and used to calculate absolute T < /b> cell numbers (Total T < /b> cell number times % viable CD3+) Acknowledgements The authors would like to thank Dr Robert Vonderheide from the...
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Solar Cells New Aspects and Solutions Part 1 doc
... of P3HT With the help of cathode confinement in the thermal treatment, a better optical absorption and a more ideal bicontinuous interpenetrating networks can be obtained at the same time This ... compared to the sample without the cathode confinement (d in Fig 14) It is well known that the absorption capability of P3HT:PCBM system is directly related to the P3HT crystallites The crystallization ... P3HT:PCBM blend film with thermal treatment (c) Al covered P3HT:PCBM blend film without the thermal treatment (d) Al covered P3HT:PCBM blend film with the thermal treatment done before cathode...
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Solar Cells New Aspects and Solutions Part 2 pptx
... Uttar Pradesh Textile Technology Institute, Souterganj, Kanpur, India Introduction In recent years alternative renewable energies including that obtained by solar cells < /b> have attracted much attention ... and stationary electronic devices to communicate, lighten, cool and heat, etc by converting sun light into electrical energy The photovoltaic materials can be integrated onto the textile structures ... laminated onto a textile substrate followed by plasma treatment and coating of PEDOT electrode Subsequent screen printing of the active material and evaporation of the ultimate electrode finished the...
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Solar Cells New Aspects and Solutions Part 3 docx
... constant than the substrate in-plane lattice parameter of the growth material will coherently strain in order to match the atomic spacing of the substrate The elastic energy of deformation due to ... misorientation of the substrate and the difference of lattice constants between substrate and the epitaxial layer In the case of flat substrate, the supersaturation increases until surface nucleation of ... to the misfit in lattice constant destroys the epilayer lattice The substrate is sufficiently thick and it remains unstrained by the growth of the epitaxial layer If the film is thin enough to...
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Solar Cells New Aspects and Solutions Part 4 ppt
... bounded by the Coulomb attraction at the donor acceptor interface, which would not contribute to photocurrent The charge carrier transport (collection) to the electrodes is the third important limiting ... directly in the polymer matrix First attempts have been performed with a one pot synthesis of PbS in MEH-PPV by Watt et al (Watt et al 2005) Although the size distribution and concentration of ... relevant differences to the AAO template is that TiO2 itself is a semiconductor, while Al2O3 is an insulator, and that the pores in the TiO2 template are closed at the bottom towards the ITO and...
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Solar Cells New Aspects and Solutions Part 5 docx
... mobility and photoconductivity in the anatase structure exceed those in the rutile structure (Tang et al., 1994) It is difficult to forecast how the crystal structure of the TiO2 matrix will be ... determined through the variation of the lattice constant Figure 2-5 depicts the lattice constant of the Ti1-xGexO2 solid solution as a function of x Here, the lattice constant of the tetragonal system ... irrespective of x At 1mol%, the lattice constant at the PbSe (220) peak is estimated to be 0.6118nm, close to that of the bulk result (Fig 3-2) This result suggests that there is also a narrow solubility...
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Solar Cells New Aspects and Solutions Part 6 potx
... have to contact continuously and simultaneously with electrode This can be accomplished by immobilization of NR in graphite felt electrode based on the data that most of bacterial cells < /b> tend to build ... Solutions Some anaerobic bacteria (Hydrogenophaga sp and Clostridium sp.) that may be originated from the anaerobic wastewater treatment reactor are detected at the initial cultivation time but ... considered to be related to the nucleation rate, cluster growth, and crystal growth To investigate the initial stage of nucleation and cluster growth, different current densities with the same deposition...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25