acids peptides and proteins
amino acids, peptides and proteins in organic chemistry
... Edited by Andrew B Hughes Amino Acids, Peptides and Proteins in Organic Chemistry Further Reading Pignataro, B (ed.) Drauz, K., Gröger, H., May, O (eds.) Ideas in Chemistry and Molecular ... (Softcover) Edited by Andrew B Hughes Amino Acids, Peptides and Proteins in Organic Chemistry Volume - Protection Reactions, Medicinal Chemistry, Combinatorial Synthesis The Editor Andrew B Hughes ... Glycopeptides and Glycopeptide Vaccines 325 Synthesis of Tn, T, Sialyl-Tn, and Sialyl-T Glycosylated Amino Acid Building Blocks 325 Synthesis of Tn, T, Sialyl-Tn, and Sialyl-T Glycopeptides and...
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hplc of peptides and proteins, methods and protocols
... material The type of n-alkyl ligand significantly influences the retention of peptides and proteins and can therefore be used to manipulate the selectivity of peptide and protein separations Although ... isocratic elution of peptides and proteins can rarely be achieved as the experimental window of solvent concentration required for their elution is very narrow Mixtures of peptides and proteins are therefore ... I and Hearn, M T W (1996) High resolution reversed phase high performance liquid chromatography of peptides and proteins Meth Enzymol 270, 3–26 Mant, C T and Hodges, R S (1996) Analysis of peptides...
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Báo cáo y học: "The use of citrullinated peptides and proteins for the diagnosis of rheumatoid arthritis" pps
... Innala and colleagues [41] 210/102 98.0 80.4 69.0 ND ND Mutlu and colleagues [37] 93/83 98.8 57.0 to 60.2l,f 60.2g 29 ND 48.4 Soos and colleagues [52] 119/118 95 74.8 ND 69.7 ND 33.6 Vander Cruyssen ... (%) 124/158 95.2 73.1 92.3 70.5 76.7 88.2 Coenen and colleagues [44] 133/165 Correia and colleagues [39] 86/90 Dos Anjos and colleagues [40] Liu and colleagues [33] CCP2 CCP3 a a 89.7 to 91.4 87.1 ... 77.4 Lutteri and colleagues [24] 120/170 77.9 79.5 Sghiri and colleagues [29] 170/309 91.1 86.3 66.0 84.7 58.6 79.9 Soos and colleagues [52] 119/118 97.6 74.4 90.0 78.8 80.2 74.0 Ursum and colleagues...
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Characterization of new peptides and physiological amino acids present in cerebrospinal fluid of chronic pain patients
... classification and molecular mechanisms of pain, role of amino acids and known peptides in pain perception mechanisms 36 Chapter Two A Simple Quantitative HPLC method for measuring Physiologic amino acids ... system Some of the transmitters and receptors involved in pain transmission or modulation, especially peptides, excitatory amino acids (EAA) and inhibitory amino acids (IAA) are widely distributed ... with 30, 35 and 41 residues were identified in human, rat and mouse brains respectively (Lee et al., 1999) NST also attenuated the allodynia and hyperalgesia evoked by prostaglandin E2 and the inflammatory...
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Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx
... without amino acids and agar were from BD (Franklin Lakes, NJ, USA) Ammonium sulphate and amino acids were from Merck (Darmstadt, Germany) Strains, plasmids and primers The strains and plasmids ... Leu2F and Leu2R (supplementary Table S1) These two primers contained 20 nucleotides that matched the 5¢- and 3¢-sequence of LEU2, and 40 nucleotides with a sequence identical to the 5¢- and 3¢-sequences ... mm Mg(CH3COO)2, 100 mm KCl and mm dithiothreitol, and suspended in the same buffer containing mm PMSF and 4000 U RNasine The cell suspension was mixed with glass beads and the yeast cells lysed...
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Báo cáo khoa học: Evolutionary origin and divergence of PQRFamide peptides and LPXRFamide peptides in the RFamide peptide family pdf
... RFamide peptides T Osugi et al annelids, molluscs, and arthropods Invertebrate RFamide peptides produced within the nervous system can act as neurotransmitters and neuromodulators These neuropeptides ... years ago, and they therefore serve as a key animal in understanding the evolutionary history of PQRFamide and LPXRFamide peptides Here, we show that the lamprey brain possesses PQRFamide peptides ... al Novel lamprey RFamide peptides Fig Unrooted phylogenetic tree of the precursors of the identified lamprey PQRFamide peptides, and the identified and putative RFamide peptides in other vertebrates...
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Báo cáo Y học: Modulation of the oligomeric structures of HIV-1 retroviral enzymes by synthetic peptides and small molecules pptx
... Structure and function of HIV-1 PR HIV-1 PR catalyzes the hydrolysis of specific peptide bonds within the HIV-1 Gag and Gag-Pol polyproteins to generate the various structural and functional proteins ... from the a1 and a5 helices of both subunits Synthetic peptides corresponding to the respective sequences (93–107 and 167– 187) were found to strongly inhibit the 3¢-processing and strand-transfer ... (Eur J Biochem 269) 5105 Table Peptide and small molecule inhibitors of HIV-1 PR dimerization IC50 (lM) Peptides Derived from the Nand C- Termini of HIV-1 PR and MA Ac-Thr-Leu-Asn-Phe-COOH N-Pro-Gln-Ile-Thr-Leu-Trp-OH...
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DNA, RNA and proteins
... DNA and Protein Synthesis • DNA contains the genetic information to make amino acids • Amino acids combine to make proteins • These proteins determine the physical traits of an organism and control ... and control cellular functions • Proteins everything, and DNA gets all the credit! • Think of them as tiny minions who all the work DNA and Protein Synthesis DNA and Protein Synthesis • Codon: ... ribosomes (rRNA) in the cytoplasm DNA and Protein Synthesis – Codons and amino acids Name the Amino Acid: UAU Tyrosine CCG Proline AGU Serine GCA Alanine DNA and Protein Synthesis But… How does...
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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx
... conserved negatively charged amino acids and cysteines Tagging of Ntcp mutants by the FLAGÒ motif To determine whether the wild-type and the mutant proteins are expressed and located on the surface of ... basolateral (Ntcp rat [1], Ntcp mouse1 and [9], Ntcp rabbit [24], and NTCP human [2]) and four apical (Isbt rat [6], Isbt mouse [25], Isbt rabbit [26] and ISBT human [27]) was performed, using ... Probes, Leiden, Netherlands) They were again washed with NaCl/Pi and embedded in Technovit 7100 (Heraeus Kulzer, Wehrheim, Germany) Sections, 5-lm thick, were cut and proteins were detected by...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... The ligand-dependent interaction between AR NTD and AR LBD, N/C interaction, was studied in yeast and mammalian in vivo protein interaction systems, and in Ó FEBS 2002 Interaction between androgen ... amino acids 23, 26 and 27 completely abolished interaction with GalDBD-AR.LBD and alanines at positions 24 and 25 increased the interaction capacity All alanine substitutions of amino acids flanking ... pr24–39sense, and pr24–39antisense Primers pr18/19AA, pr20/21AA, pr22A, pr24/25AA, pr26/27AA, pr28/29AA, and pr30/ 31AA sense and antisense oligonucleotides were modified pr17–32 sense and antisense...
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Báo cáo Y học: Functional analysis of the rat bile salt export pump gene promoter Regulation by bile acids, drugs and endogenous compounds potx
... and b-estradiol; 50 lmolÆL)1 each of phenobarbital, rifampin, and tamoxifen Assay conditions were the same as described in Fig *P < 0.05 the specific band Supershifted bands were rather weak and ... indicates the ability of drugs and endogenous compounds to affect Bsep transcription, thus altering hepatic bile flow and clearance MATERIALS AND METHODS Genomic cloning and sequence analysis of the ... polyacrylamide gel and autoradiographed For supershift experiments nuclear extracts were preincubated with polyclonal antibodies against either FXRa (lane and 4), RXRa (lane and 6) or both (lane and 8) prior...
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Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
... of fluorescent proteins by arginine-rich intracellular delivery (AID) and Tat-protein transduction domain has been reported in onion and corn root tip cells [28,29] Cationic oligopeptides such ... have demonstrated noncovalent transduction of 27 kDa fluorescent proteins by Tatprotein transduction domain and AID proteins in corn and onion root tip cells In the present study, we demonstrate ... : 1, : and : of Tat2 and plasmid DNA However, before mixing, Tat2 and the DNA were prepared separately in 25 lL of sterile water The mixture was incubated for h for complex formation and subjected...
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Báo cáo sinh học: " Discovery of herpesviruses in multi-infected primates using locked nucleic acids (LNA) and a bigenic PCR approach" pdf
... CgueCMV-1.1 and CgueCMV-1.2 (B) Pairwise alignments of the UL56 and gB proteins and the partial DPOL proteins of CgueCMV-1.1 and CgueCMV-1.2 are shown Dots represent identical amino acids, dashes ... ORFs UL56 and UL55 (gB), and two thirds of the ORF UL54 (DPOL) (Figure 6B) The nucleotide and amino acid sequence differences of CgueCMV-1.1 and CgueCMV-1.2 were 0.6% and 0.5% (UL56), 20% and 8% ... found in the kidney and brain and the other in liver and mucosa (CMV-1 and CMV-2, respectively) They had a nucleotide sequence identity of 82% With LD-PCR, the CMV-1 gB sequence and the CgueCMV-1...
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Báo cáo toán học: " Improved labelling of DTPA- and DOTA-conjugated peptides and antibodies with In-111 in HEPES and MES buffer" ppt
... Improved labelling of DTPA- and DOTA-conjugated peptides and antibodies with 111In in HEPES and MES buffer Maarten Brom*1, Lieke Joosten1, Wim JG Oyen1, Martin Gotthardt1 and Otto C Boerman1 Department ... DTPA-octreotide and DOTA-anti-CAIX antibody were five times higher in MES and HEPES buffer Radiolabelling of DTPA-exendin-3, DOTA-exendin-3 and DTPA-anti-CAIX antibody in MES and HEPES buffer ... than that in NaAc and NH4Ac Labelling of DTPA-exendin3 decreased with 66% and 73% for NaAc and NH4Ac, respectively, at day 11 after the production date of 111InCl3, while for MES and HEPES, the...
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báo cáo hóa học:" Improved labelling of DTPA- and DOTA-conjugated peptides and antibodies with In-111 in HEPES and MES buffer" doc
... Improved labelling of DTPA- and DOTA-conjugated peptides and antibodies with 111In in HEPES and MES buffer Maarten Brom*1, Lieke Joosten1, Wim JG Oyen1, Martin Gotthardt1 and Otto C Boerman1 Department ... DTPA-octreotide and DOTA-anti-CAIX antibody were five times higher in MES and HEPES buffer Radiolabelling of DTPA-exendin-3, DOTA-exendin-3 and DTPA-anti-CAIX antibody in MES and HEPES buffer ... than that in NaAc and NH4Ac Labelling of DTPA-exendin3 decreased with 66% and 73% for NaAc and NH4Ac, respectively, at day 11 after the production date of 111InCl3, while for MES and HEPES, the...
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Báo cáo hóa học: " A Genetic Programming Method for the Identification of Signal Peptides and Prediction of Their Cleavage Sites David Lennartsson" pot
... training set consisting of 176 background proteins and 291 signal peptides and one validation set consisting of 75 background proteins and 125 signal peptides For every position in the peptide ... extraction and preparation of the dataset, see [2, 7] Nielsen et al performed their experiment on several different groups of proteins including human, E coli, eukary- 139 otes, and gram+ and gram− ... Figure The peptide sequences were truncated after 70 amino acids for background proteins In the case of signal peptides, the signal part and the first 30 positions of the mature protein were kept...
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Báo cáo y học: " Diagnostic value of anti-cyclic citrullinated peptides and association with HLA-DRB1 shared epitope alleles in African rheumatoid arthritis patients" ppsx
... Zurich, Switzerland), were used to detect IgM and IgA RF The assays and calculations were performed according to the manufacturer’s instruction Each kit included its own RF standard and the results ... Switzerland Authors’ contributions MSN recruited the patients and collected the samples MSN and CG designed the study SB and J-MT performed the analysis AF performed the statistical analysis MSN and ... Switzerland), and those detecting the IgG anti-CCP3 (Quanta Lite CCP3: third generation of CCP antigen), were purchased from Inova Diagnostics (Ruwag, Zurich, Switzerland) The assays and calculations...
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Báo cáo y học: "Flexible peptides and cytoplasmic gels" pptx
... serotonin, and GABA [20] In all these cases, protein structure is in fact loosened by ligand binding, and a relatively weak association is driven mainly by entropic changes Williams and colleagues ... Stephens E, Zhou M: Ligand binding energy and catalytic efficiency from improved packing within receptors and enzymes J Mol Biol 2003, 329:389-399 20 Williams DH, O’Brien DP, Sandercock AM, Stephens ... regions, one long and the other short Most impressive of all, the carboxy-terminal regions of the major receptor types, Tar and Tsr, consist of a length of perhaps 30 amino acids that so far...
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Computational techniques for simulating the interactions between peptides and carbon nanotubes
... nanotubes (CNTs) and peptides 1.1.1 General overview of CNTs 1.1.1.1 Molecular structure of CNTs 1.1.1.2 Properties of CNTs and their applications 1.1.2 Proteins and peptides ... diagnostic devices 1.1.2 Proteins and peptides Proteins are building blocks of a living cell, and they participate in essentially all cellular processes One of the major functions of proteins is enzymatic ... acids in the protein chain Most natural polypeptide chains contain between 50 and 2000 animo acid residues and are usually referred to as proteins Polypeptides made of small number of amino acids...
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