... primers: Aba, 5Â-ATGGACGCTGAATTCCGTCACGACTCTGGTTACGAAGTTCACCACCAGAAGCTGGTG-3Â;Abb, 5Â-GTTCACCACCAGAAGCTGGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAGGGTGCT-3Â;Abc, 5Â-CACAACGCCACCAACCATCAGACCGATGATAGCACCCTTGTTAGAACCCAC-3Â;Ab-start, ... 5Â-CACAACGCCACCAACCATCAGACCGATGATAGCACCCTTGTTAGAACCCAC-3Â;Ab-start, 5Â-GCGTAGGGTCGACATATGGACGCTGAATTCCGTCACG-3Â;Abstop, 5Â-CCTGCCGAGCTCCTATTACACAACGCCACCAACCATCAG-3Â.The PCR solution was prepared in the buffer suppliedwith ... (F19P, A2 1G, E22G, E22K, E22Q andD23N), and the availability of a rapid and simpleexpression and purification protocol will facilitatelarge-scale investigations of the molecular determinantsof aggregation...
... tsA58T Ag cDNA carry-ing the A4 38V mutation were PCR-amplified from COS-7cDNAs using the following primers: LTA-1F, 5Â-CTCGAGATGGATAAAGTTTTAAACAGAG-3Â and LTA-1R, 5Â-TGAAGGCAAATCTCTGGAC-3Â for ... isolation and long-termculture of organ-specific blood vascular and lymphaticendothelial cells of the mouseTakashi Yamaguchi, Taeko Ichise, Osamu Iwata, Akiko Hori, Tomomi Adachi, Masaru Nakamura,Nobuaki ... Oreda Bet al. (20 03) The conditional inactivation of thebeta-catenin gene in endothelial cells causes a defectivevascular pattern and increased vascular fragility. J CellBiol 1 62, 1111–1 122 .8...
... 605–613,Ann Arbor, June 20 05.c 20 05 Association for Computational Linguistics A Nonparametric Methodfor Extraction of Candidate Phrasal Terms Paul Deane Center for Assessment, Design and Scoring ... Annual Meeting of the Association for Computational Linguistics, pages 188-195. Ferreira da Silva, J. and G. Pereira Lopes (1999). A local maxima method and a fair dispersion normalization for ... unigram distributions, and generalizations to bigram and n-gram distributions on large corpora are not as yet clearly feasible (Baayen 20 01 :22 1). Yet many of the best-performing lexical association...
... ocean. Some are mentioned as being able to divide the waters at their will, and to cause them toreturn to their natural place, merely by means of a short formula. An image of a man or animal made ... or a she-ass.Smaller retail bargains did not demand so many or such complicated calculations. Two townsfolk stop for a moment in front of a fellah who offers onions and corn in a basket for sale. ... celebrated lawsuit, some details of which are preserved for usin a papyrus of Turin, gives us some information in regard to a conspiracy which was hatched in the haremagainst Ramses II.** A passage...
... problemsAppendix C. Basic equations of displacement analysisAppendix D. Some integration formulae fora triangleAppendix E. Some integration formulae fora tetrahedronAppendix F. Some vector algebraAppendix ... explicitdynamic transient analysis (see Chapter 18 of Volume 1) is carried out to achieve a steady-state solution. 32; 33These forms are often characterized by:1. a diagonal or very sparse form of the matrix ... of transient non-linear calculations 19 disadvantage of a line search is the need for several evaluations of É. However, theacceleration of the overall convergence can be remarkable when applied...
... to develop and use chemical agents, Aum’s early struggles againstrival organizations reveal how it managed relations with others whom Asahara andhis close associates viewed as obstacles to their ... group are particularly significant: First, Aum was the crea-tion of Shoko Asahara, its founder, charismatic leader, and spiritual guru. Second,Aum learned how to raise money, ultimately amassing ... contacts thatit eventually exploited to procure weapons capabilities. For example, Aum had twocompanies that purchased chemicals for its chemical weapons programs without at-tracting unwanted...
... physiologically feasibleranges:1 2 k0ATPase kATPase 2k0ATPase(small variation of the energetic load)15k0ATPase kATPase 5k0ATPase(large variation of the energetic load)150k0ox ... tissue [25 ,26 ]. Finally, ifregulatory effectors have been elucidated by carefulkinetic characterization of an enzyme, there are suffi-cient data available to set up a mechanistic rate lawinstead ... notinclude all reactions that have been reported in theTable 5. Ranking of saturation parameters for hepatocyte purinemetabolism. Average ranking of saturation parameters according totheir impact...
... A hierarchical Bayesian lan-guage model based on Pitman-Yor processes. InProceedings of COLING-ACL 20 06, pages 985–9 92. Akira Terada, Minoru Yoshida, and Hiroshi Nakagawa. 20 04. A tool for constructing ... Estimation of Distributional SimilaritiesJun’ichi Kazama Stijn De Saeger Kow KurodaMasaki Murata†Kentaro TorisawaLanguage Infrastructure Group, MASTAR ProjectNational Institute of Information ... 48th Annual Meeting of the Association for Computational Linguistics, pages 24 7 25 6,Uppsala, Sweden, 11-16 July 20 10.c 20 10 Association for Computational Linguistics A Bayesian Methodfor Robust...
... conjuncts may be changed. The unconditional conjuncts of a formula contain informa- tion that is more definite than the information contained in disjunctions. Thus a formula can be regarded as having ... Press, Cam- bridge, England, 1985. [8] Perelra, F. C. N. and D. H. D. Warren. Definite clause grammars for language analysis - a survey of the formal- ism and a comparison with augmented transition ... disjunctions, if any at all. Most disjunctions in a systemic grammar represent possible alternative values that some par- ticular feature may have (along with the grammatical conse- quences entailed...
... the annealed titania nanotubesare crystalline (mostly anatase) and show varied activity de-pending on the material preparation and annealing atmosphere(Fig. 12) .Titania nanotubes prepared ... stirring and (b) ultrasonic. S.K. Mohapatra et al. / Journal of Catalysis 24 6 (20 07) 3 62 369 367Fig. 10. DRUV–vis spectra of (a) O 2 annealed UAT, (b) N 2 annealed UAT,(c) as-prepared UAT, and ... respectively. 2. 3. Annealing of the materialsThe anodized titania nanotubular arrays were annealed in a nitrogen and oxygen atmosphere at 500◦Cfor6hinaCVDfur-nace at a heating rate of 1◦C/min. The UAT samples...
... Cancer 20 08, 122 :25 62- 7. 22 . Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N,Hase T: Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 20 00, 28 :E63. 23 . Weitz ... sequences for amplification of CEA were: 5’ -AGACAATCACAGTCTCTGCGGA-3’ (forward) and5’ - ATCCTTGTC CTCCACGGGTT-3’ (reverse). Thecut-off was set at cycle time = 29 .6 for CK19, 28 .5 for CEA, and 30.0 ... levels for each of 2 LN slices). RNA quality wasassured by OSNA performed for beta-actin. 139 samplesgaveanegativeresultand37samplesgaveapositiveresult with both methods (table 2) . No isolated...
... times a week.The database will be made available free of charge to inter-ested parties.Table 7: Amplification efficiency for patients' ampliconsGenotype 1a Amplicon A1 A2 A3 A4 x A4 yAmplification ... K: Hypervariable regions in the putative glycoproteinof hepatitis C virus. Biochem Biophys Res Commun 1991,175 :22 0 -22 8.8. Kato N, Ootsuyama Y, Tanaka T, Nakagawa M, Nakazawa T, MuraisoK, Ohkoshi ... sequences and conservedsecondary structures at the 3' end of HCV genome and itsimplication for viral replication. Nucleic Acids Res 19 92, 20 :3 520 .7. Hijikata M, Kato N, Ootsuyama Y, Nakagawa...