a a and rn
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... DNA (A) EMSA was performed with EWS (lanes and 4) and 32P-labeled ETS-1 (lanes and 4) or ssDNA L (lanes and 2) (B) EMSA was performed with EWS (lanes 2, and 6) and 32P-labeled Htelo (lanes and ... ACA G) and EWS reverse d(CGC TCG AGT CAC TAG TAG GGC CGA TCT CTG C), for pGEX–EWS; EAD forward d(CGG AAT TCA TGG CGT CCA CGG ATT ACA G) and EAD reverse d(CGC TCG AGT CAT CCG GAA AAT CCT CCA GAC ... RGG3 (lanes and 4) and 32P-labeled Htelo (lanes and 2) or rHtelo (lanes and 4) (B) EMSA was performed with RGG3 (lanes and 4) and 32P-labeled rHtelo (lanes and 2) or mut rHtelo (lanes and 4)...
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Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
... primers (forward, 5¢-TAATACGACTCACTATAGGTACTATG TATCGCATGCCAAT-3¢; reverse, 5¢-TAATACGACTC ACTATAGGTACTTTAAAGTCCCGGGTTGA-3¢) For PCR, the reaction mix consisted of · Taq buffer containing 1.5 mm MgCl2, ... either: (a) dsRNA; or (b) small interference (si)RNA The longer dsRNA may generate a large population of siRNA (with 21–23 nucleotides), and the use of longer dsRNA may be advantageous over siRNA In ... successful RNAi has been reported for many animal models For example, Caenorhabditis elegans can be soaked in dsRNA or can be fed plasmids that make dsRNA and consequently exhibit RNAi effects In many...
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Báo cáo khoa học: A structured RNA in hepatitis B virus post-transcriptional regulatory element represses alternative splicing in a sequence-independent and position-dependent manner pot
... unspliced pgRNA and the SP1 splicing variant of HBV, primers SP1 (tgcccctatcctatcaacac), SP2 (actcccataggaattttccgaaa) and U2 (ttccaatgaggattaaagacag) were used Quantification of the splicing ratio by ... analysis of the fragments of the 5¢ end labeled and folded PRE-ISS wild-type RNA, which was cleaved by RNase T1, RNase V1 and RNase A The T1 seq and A seq lanes represent RNase T1 and RNase A ... Triangles, diamonds and ellipses represent signals for RNase T1, RNase V1 and RNase A, respectively; larger ones represent stronger signals and smaller ones weaker signals (C) Conservation analysis...
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Báo cáo sinh học: " Open Access Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" docx
... TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG Consensus (1851) TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG ... Rat TATTGGACCTGGAGATAGGTACTGACACGC Mouse TTTGGGCCGCCGGGTTATATGCTGACACGC 216 bases TGCCTTATATGTTCGTCTGTAGGAGCGAGT GCATGTGCGGGCAGGAAGGTAGGGGAAGAC GATC Drosophila TATTGTACCTGGAGATATATGCTGACACGC 726,713 ... Caledonia/20/1999 ca A/ Solomon Island/3/2006 MDV -A (ca A/ Ann Arbor/6/1960) ca A/ Wisconsin/67/2005 ca A/ California/7/2004 ca A/ Panama/2007/1999 ca A/ Hong Kong/213/2003 ca A/ Hong Kong/1997(491 H5/486 N1) ca A/ Vietnam/1203/2004...
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Báo cáo sinh học: " Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinant" doc
... gel and stained with ethidium bromide Abundant ribosomal RNAs 28S and 18S are indicated B and C: PKR knockout (PKR-/-) and PKR WT cells (PKR+/+) (panel B), as well as RNase L knockout (RL-/ -) and ... principle, and uses mouse monoclonal antibodies directed against DNA and histones to estimate the amount of cytoplasmic histoneassociated DNA fragments Total RNA isolation Total RNA from uninfected ... regulation Adv Virus Res 1993, 42:57-102 Hassel BA, Zhou A, Sotomayor C, Maran A, Silverman RH: A dominant negative mutant of 2- 5A- dependent RNase suppresses antiproliferative and antiviral effects...
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Báo cáo hóa học: " Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" pdf
... TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG Consensus (1851) TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG ... Rat TATTGGACCTGGAGATAGGTACTGACACGC Mouse TTTGGGCCGCCGGGTTATATGCTGACACGC 216 bases TGCCTTATATGTTCGTCTGTAGGAGCGAGT GCATGTGCGGGCAGGAAGGTAGGGGAAGAC GATC Drosophila TATTGTACCTGGAGATATATGCTGACACGC 726,713 ... Caledonia/20/1999 ca A/ Solomon Island/3/2006 MDV -A (ca A/ Ann Arbor/6/1960) ca A/ Wisconsin/67/2005 ca A/ California/7/2004 ca A/ Panama/2007/1999 ca A/ Hong Kong/213/2003 ca A/ Hong Kong/1997(491 H5/486 N1) ca A/ Vietnam/1203/2004...
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báo cáo hóa học:" Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinant" docx
... gel and stained with ethidium bromide Abundant ribosomal RNAs 28S and 18S are indicated B and C: PKR knockout (PKR-/-) and PKR WT cells (PKR+/+) (panel B), as well as RNase L knockout (RL-/ -) and ... principle, and uses mouse monoclonal antibodies directed against DNA and histones to estimate the amount of cytoplasmic histoneassociated DNA fragments Total RNA isolation Total RNA from uninfected ... regulation Adv Virus Res 1993, 42:57-102 Hassel BA, Zhou A, Sotomayor C, Maran A, Silverman RH: A dominant negative mutant of 2- 5A- dependent RNase suppresses antiproliferative and antiviral effects...
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Báo cáo y học: " Dual role of TRBP in HIV replication and RNA interference: viral diversion of a cellular pathway or evasion from antiviral immunity?" potx
... specificity of action [10] Adenovirus VA RNAI and VA RNAII are cleaved by Dicer and act as RNAi suppressors [13] Both Tat protein and VA RNAs inhibit Dicer activity A striking feature of RNAi suppressors ... translation and replication and as a consequence becomes unavailable to bind Dicer and mediate RNAi In this case, both TRBP and Tat would participate in the inhibition of HIV restriction by RNAi ... Lancaster AM, Lemon SM, Sarnow P: Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA Science 2005, 309:1577-1581 Sheldon M, Ratnasabapathy R, Hernandez N: Characterization...
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optimization of protein and rna detection methodologies and a new approach for manipulating protein activity in living cells
... TTTTGCGGCCGCCMNNMNNMNNGCAGCAGCCGGGGCAGCA AATACGACTCACTATAGGGA GTAATCCAGAGGTTGATTCTCGAGAAAA TTTTAAGCTTGCCACCATGGCCGGATCCTAAGCGGCCGCAGCAAGGGCGAGGAG CTG 10 CCCCATCGATCTCGAGTTACTTGTACAGCTCGTCCAT 11 ACCTACAGGTGGGGTCTTTCATTCCC ... 12 AGCTCGTTTAGTGAACCGTCAGATC 13 GACAAGCGGCCGCTTAAGAACCGC 14 AAACTCGAGTTAGCGGCCGCCCCTCCACATGCAG 15 AAAGCGGCCGCCAGAACCGCAGCACCCGGGGCA 16 TTTGCGGCCGCATGGATGATGATATCGCCGCG 17 TTTCTCGAGCTAGAAGCATTTGCGGTGGAC ... TTTCTCGAGCTAGAAGCATTTGCGGTGGAC 18 CTCAGATCTCGGGCTATGGATGATGATATCGCCGC 19 TCGAGATCTGAGTCCGGACTTGTACAGCTCGTCCATG 20 TTTAAGCTTGCCACCATGGATTACAAGGATGACGACGATAAGGGATCCGCCGGAT CCTTTTTGAATTG 32 Table 1.2 Continued 21 TTTAAGCTTGCCACCATGGTGTACCCCTACGACGTGCCCGACTACGCCGGATCCG...
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600 sentences of certificate A and B
... desk a the b a c an d some > c 134 What is color of your pen? a the b a c an d any > a 135 Kate and Mary are going to cinema a the b a c an d no article > a 136 My parents are always at ... southern countries a Sun b always c on d in > a 52 The man that wife and family are away seems very lonely a that b and c are d seems > a 53 Each year more and more people try setting new and ... communication > c 72 Though TV, home viewers can see and learn about people, places and things in faraway lands a Though b learn c about d in > a 73 TV provides many more entertainment programs as...
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Test yourself A and Test 1(Kiều Tính)
... climbing, backpacking, and adventure tourism Some recreational activities are made illegal such as gambling and drug use Research has shown that recreation contributes to life satisfaction, quality ... life, health and wellness, and that the use of recreation as a diversion may have clinical applications to individuals with chronic pain and other health impairments In some cultures and religions, ... "the day of rest." Holiday is also a common time for recreation Traditionally, music and dance serve as recreation in many cultures Playing sports, hobbies, games and tourism are popular forms...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... TCCTGGCAATCGTGGTT CAA and ACCAGCTGGGCCAACATTTC; collagen III: TGGACAGATGCTGGTGCTGAG and GAAGGCCAG 3696 CTGTACATCAAGGA; alpha smooth muscle actin (a- SMA): AGCCAGTCGCCATCAGGAAC and CCGG AGCCATTGTCACACAC; ... 5¢-GCTGTGGCAGCTACCTATGTCTTG-3¢ and 5¢-AGGTCGTCATCATCCCACGAG-3¢; TNF -a: 5¢-AACTCGAGTGACAAGCCCGTAG-3¢ and 5¢-GTAC CACCAGTTGGTTGTCTTTGA-3¢; IL-10: 5¢-CAGACCC ACATGCTCCGAGA-3¢ and 5¢-CAAGGCTTGGCAA CCCAAGTA-3¢; collagen I: ... AGCCATTGTCACACAC; and glyceraldehyde-3-phosphate dehydrogenase: 5¢-GGCACAGTCAAGGCTGAGAATG-3¢ and 5¢-ATGGTGGTGAAGACGCCAGTA-3¢ Immunocytochemical staining for NF-jBp65 MSCs in IMDM supplemented with 10% fetal...
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... supernatants after 24 h of incubation FEBS Journal 276 (2009) 24022415 ê 2009 The Authors Journal compilation ê 2009 FEBS G Vaaje-Kolstad et al Degradation of a- and b-chitin The degradation rates ... containing a putative transcription regulator (GenBank ID: AAK06047.1), chitinase gene (GenBank ID: AAK06048.1) and gene encoding a family 33 CBP (GenBank ID: AAK06049.1) was amplied FEBS Journal ... initial phase was maintained longer than in the absence of LlCBP3 3A, indicating that LlCBP3 3A acts synergistically with LlChi1 8A However, the effect of LlCBP3 3A was small and ceased after approximately...
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Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf
... b1,3galactosidases, giving rise to a disaccharide and a monosaccharide, and was thus identified as a mixture of Galb1-4[Fuca1-3]GlcNAcb1-3Gal and Galb1-3[Fuca1-4]GlcNAcb1-3Gal The calculated amounts ... b1,4galactosidases, giving rise to a disaccharide and a monosaccharide, and is thus identified as a mixture of Galb1-3GlcNAcb1-3Gal and Galb1-4GlcNAcb1-3Gal The tetrasaccharide is sensitive to a1 ,3/4 ... large O-glycans contain a minimal amount of radioactivity and were not analysed further, whereas those released from T5AS clone mostly show a disaccharide peak and a smaller trisaccharide peak...
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Tài liệu Báo cáo khoa học: The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression ppt
... section and Fig 1) The RNP and RNP motifs are located in the central strands of the b-sheet, namely b3 and b1, respectively, and are highly conserved apart from a few RRM domains such as ALY and TAP ... domain, a plastic RNA-binding platform RNA-binding affinity and specificity and diversify its biological functions A protein domain in such abundance is necessarily biologically important and associated ... domain and another domain In some cases, it has been demonstrated that RRMcontaining proteins can associate with RNA only in the presence of another protein that acts as a cofactor Both U2B¢ and...
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Báo cáo khoa học: Estrogen-related receptor a and PGC-1-related coactivator constitute a novel complex mediating the biogenesis of functional mitochondria potx
... synthase subunit b: 5¢-CCTTCTGCTGTGGGCTATCA-3¢ and 5¢TCAAGTCATCAGCAGGCACA-3¢; ND5: 5¢-TAACCCC ACCCTACTAAACC-3¢ and 5¢-GATTATGGGCGTTGA TTAGTAG-3¢; b-globin: 5¢-CAACTTCATCCACGTTCA CC-3¢ and 5¢-ACACAACTGTGTTCACTAGC-3¢ ... 5¢-AAGACAGCAGCCCCAGTGAA-3¢ and 5¢-ACACCCAGCACCAGCACCT-3¢; PRC: 5¢-CACTGG TTGACCCTGTTCCT-3¢ and 5¢-GTGTTTCAGGGCTTC TCTGC-3¢; Cyt c: 5¢-CCAGTGCCACACCGTTGAA-3¢ and 5¢-TCCCCAGATGATGCCTTTGTT-3¢; ATP ... quantification was performed by nonsaturating picture scanning by a gel Doc 1000 Molecular Analyst apparatus (Biorad) Respiratory parameters and respiratory ratio in intact cells Respiratory parameters and...
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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
... FITC was measured in the FL1 channel (510–535 nm bandpass filter) Data were recorded and analyzed with flowmax software from Partec Statistical analysis of ELISA experiments Each experiment was repeated ... FnBRA lacking FnBPA-9 and FnBPA-10 (A, C) Recombinant, His-tagged full-length FnBRA and FnBRA lacking FnBPA-9 and FnBPA-10 (FnBRAD9,10) were immobilized on microtiter wells (1 lg in 100 lL) and ... 4501 FnBPA and FnBPB from S aureus G Provenza et al (BioRad, Hercules, CA, USA) For the western blot assay, FnBRA, FnBRB and the corresponding single repeats were separated by SDS ⁄ PAGE, and then...
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Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces significant structural changes doc
... distorted chair The C-terminal a- domain is characterized by an adamantane-like four-metal cluster Solution structures of 113 Cd-substituted Cd7MT-2 from rabbit, rat and human are available and revealed ... (Tubingen, Germany), and all ¨ other chemicals were from Merck (Darmstadt, Germany) Synthesis of the individual a- and b- domains The individual a- and b-domains (KSCCSCCPVGCSKCA QGCVCKGAADKCTCCA and MDPNCSCSTGGSCTCT ... a high-resolution solution structure of the C-terminal a- domain has become available The data revealed a tertiary fold very similar to that of MT-1 and MT-2, except for a loop that contains an...
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Báo cáo khoa học: Puroindoline-a and a1-purothionin form ion channels in giant liposomes but exert different toxic actions on murine cells pptx
... channels, and in particular with data obtained with b-PTH showing the formation of cationselective ion channels in artificial lipid bilayer membranes and in the plasmalemma of rat hippocampal ... PTH-containing and PIN-containing crude fractions were dialyzed against deionized water and freeze-dried, and a1 -PTH, a2 -PTH and b-PTH were separated (at room temperature) by semipreparative RP-HPLC ... extracellular Ca2+ concentration on the effect of a1 -PTH (B and C) Note that in (A) only a1 -PTH produces membrane depolarization, and in (B) and (C) increasing extracellular Ca2+ concentration...
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Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
... rebinding are shown in Figs and The transient absorption decays were analyzed using a standard least-squares technique using homemade software for PC After kinetic normalization, analysis showed that ... the a and b subunits from the averaged parameters of HbA oxygenation (Table 1, Average) The association and dissociation rate constants for the b subunits are found to exceed 2.2 ± 0.3- and 3.1 ... dissociation rate constant at an insignificant change in the association rate constant (Fig 4, D2 and D1, respectively) Also, at pH 8.5, the rebinding study reveals an increase in the association and...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25