8 embryonic stem cells from parthenotes
báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), ... suggest that vascular cells derived from human ES cells may have a potential to be a source for therapeutic vascular regeneration after stroke Abbreviations ES cells: Embryonic stem cells; VEGF-R2: ... Different differentiation kinetics of vascular progenitor cells in primate and mouse embryonic stem cells Circulation 2003, 107:2 085 -2 088 Masakatsu Sone, Hiroshi Itoh, Kenichi Yamahara, Jun Yamashita...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts Science 19 98, 282 :1145-1147 Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J ... determination of death USGPO Washington DC; 1 981 Landry DW, Zucker HA: Embryonic death and the creation of human embryonic stem cells J Clin Invest 2004, 114:1 184 -1 186 Voullaire L, Slater H, Williamson ... http://www.tbiomed.com/content/2/1/25 Figure Experimental embryonic stem cell colonies derived from a single blastomere Experimental embryonic stem cell colonies derived from a single blastomere of research in...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... ISOLATION OF CHONDROGENIC CELLS …………… 86 4.3.1 4.3.2 Expansion of hESC-derived chondrogenic cells ……………… 88 4.3.3 Differentiation capability of hESC-derived chondrogenic cells … 88 4.3.4 Characterization ... relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee ... fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 20 08) Human embryonic stem cells...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 19 98; 282 (5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... 2000; 18( 4):399-404 Richards M, Fong CY, Tan S, Chan WK, Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779 -89 ... feeder layer of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington,...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes but not the embryonic and...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells derived from developing embryos ES cells derived from the ... profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing embryos ... derived from all three germ layers Teratomas, as well as fetuses, have FEBS Journal 275 (20 08) 1624–1630 ª 20 08 The Authors Journal compilation ª 20 08 FEBS 1625 Epigenetic study of embryonic stem cells...
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embryonic stem cells, methods and protocols - kursad turksen
... Chapter 33.) Murine Embryonic Stem Cells 1 Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells Marsha L Roach and John D McNeish Introduction Embryonic stem (ES) cells were rst ... Murine Embryonic Stem Cells Table Media Protocols for ES Cells and Feeder Cells Reagents sDMEM SCML G4 18/ Ganc/SCML G4 18/ SCML HAT/SCML KO-DMEM FBS L-Glutamine MEM/NEAA BME LIF Pen/Strept G4 18 Gancyclovir ... human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers Mol Med 6, 88 95 17 Reubinoff, B., Pera, M., Fong, C., Trounson, A., and Bongso, A (2000) Embryonic stem...
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differentiation of embryonic stem cells
... Megakaryocytes from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells ... AND SHIN-ICHI HAYASHI 98 v vi TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils ... Culture of Embryonic Germ Cells MARIA P DE MIGUEL AND PETER J DONOVAN 353 Section III Gene Discovery by Manipulation of Mouse Embryonic Stem Cells 26 Gene Trap Mutagenesis in Embryonic Stem Cells...
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báo cáo hóa học:" Stem cells from umbilical cord blood do have myogenic potential, with and without differentiation induction in vitro" pdf
... stem cells transdifferentiated in muscle cells in vitro CD34+ stem cells transdifferentiated in muscle cells in vitro CD34+ stem cells that transdifferentiated in dystrophin producer muscle cells ... with CD34+ stem cells and DMB affected patient muscle cells (data not shown) In order to confirm if there was any expression of dystrophin from the CD34+ stem cells, we used muscle cells from a DMD ... transdifferentiation of adherent cells obtained from prior CD34+ stem cells Nc) normal control (human skeletal muscle) SC) CD34+ stem cells aSC) adherent stem cells (prior CD34+) Emery AEH: Duchenne...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... severe skeletal defects Mol Cell Biol 2007, 27(10): 385 5- 386 7 http://www.translational-medicine.com/content/7/1/20 78 79 80 81 82 83 84 85 86 87 88 Steele-Perkins G, Plachez C, Butz KG, Yang G, Bachurski ... ovarian cancer Cancer Res 2007, 67( 18) :86 99 -87 07 Cao H, Yang CS, M RT: Evolutionary Emergence of microRNAs in Human Embryonic Stem Cells PLoS ONE 20 08, 3(7):e 282 0 Liao R, Sun J, Zhang L, Lou G, ... reported in human [16,17] and mouse embryonic stem cells [ 18- 20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs...
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báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot
... immune rejection than adult cells Stem Cells 2006, 24(2):221-9 Koch CA, Geraldes P, Platt JL: Immunosuppression by embryonic stem cells Stem Cells 20 08, 26(1) :89 - 98 Fabricius D, Bonde S, Zavazava ... molecules on murine embryonic stem cells Biol Reprod 1997, 57(3):561 -8 Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, 24(10):2192-201 ... embryonic stem cells from lysis by antigen-specific CTL J Immunol 2007, 1 78( 6):3390-9 Drukker M, Katchman H, Katz G, Even-Tov Friedman S, Shezen E, Hornstein E, et al.: Human embryonic stem cells...
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Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot
... Proteome Res 2010, 9(11): 584 8- 585 8 doi:10.1 186 /1479- 587 6-9-96 Cite this article as: Ponnikorn et al.: Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia ... hematopoietic stem cells (HSCs) Bone marrow samples (BM) from Hb E/b-thalassemic patients and healthy donors were collected for HSCs isolation Briefly, mononuclear cells (MNCs) from BM were separated ... erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according to a procedure previously described [13] with some modifications HSCs/CD34 + cells were...
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o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx
... Proteome Res 2010, 9(11): 584 8- 585 8 doi:10.1 186 /1479- 587 6-9-96 Cite this article as: Ponnikorn et al.: Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia ... hematopoietic stem cells (HSCs) Bone marrow samples (BM) from Hb E/b-thalassemic patients and healthy donors were collected for HSCs isolation Briefly, mononuclear cells (MNCs) from BM were separated ... erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according to a procedure previously described [13] with some modifications HSCs/CD34 + cells were...
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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES doc
... stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... human embryonic stem cells J Biol Chem, 20 08 283 (36): p 24991-5002 [27] Zhang, P., et al., Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells Blood, 20 08 111(4): ... pathways in human embryonic stem cells Stem Cells, 2006 24(6): p 1476 -86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells Int J Mol Med,...
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EMBRYONIC STEM CELLS – DIFFERENTIATION AND PLURIPOTENT ALTERNATIVES pdf
... stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In the book Embryonic Stem ... human embryonic stem cells J Biol Chem, 20 08 283 (36): p 24991-5002 [27] Zhang, P., et al., Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells Blood, 20 08 111(4): ... pathways in human embryonic stem cells Stem Cells, 2006 24(6): p 1476 -86 [105] Katoh, M., CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells Int J Mol Med,...
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EMBRYONIC STEM CELLS – BASIC BIOLOGY TO BIOENGINEERING doc
... 752-761 Lavon N, Narwani K, Golan-Lev T et al 20 08 Derivation of euploid human embryonic stem cells from aneuploid embryos, Stem Cells 26: 187 4- 188 2 Munne S, Velilla E, Colls P (2005) Self-correction ... Applications of Embryonic Stem Cells in Research and Development 191 Chapter 11 Methods to Generate Chimeric Mice from Embryonic Stem Cells 193 Kun-Hsiung Lee Chapter 12 Embryonic Stem Cells in Toxicological ... pp (1534-1541) 14 Embryonic Stem Cells – Basic Biology to Bioengineering Dottori, M., & Pera, M.F (20 08) Neural Differentiation of Human Embryonic Stem Cells, In: Neural Stem Cells : Methods and...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... neurons from mouse embryonic stem cells Nat Biotechnol 2000, 18, 675-679 41 Levenberg S, Golub JS, Amit M, Itskovitz-Eldor J, Langer R Endothelial cells derived from human embryonic stem cells ... and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem- derived cells Trends Biotechnol 2004, ... from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells Proc Natl Acad Sci USA 1 981 , 78, 7634-76 38 43 Martin MJ, Muotri A, Gage F, Varki A Human embryonic stem cells...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf
... pluripotency in mouse embryonic stem cells Cloning Stem Cells 2004, 6, 386 -391 17 Cobb MH, Goldsmith EJ How MAP kinases are regulated J Biol Chem 1995, 270, 1 484 3-1 484 6 18 Cochrane CG Cellular injury ... dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress 255 10, 423-426 12 Brook FA, Gardner RL The origin and efficient derivation of embryonic stem cells in the mouse ... of Ohkawa et al [ 48] The mouse ES cells were washed twice with PBS, then harvested and sonicated One hundred microliters of sonicated cells were mixed with 8% SDS (100 μl), 0 .8% 2-thiobarbituric...
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