... GAA GG 5'; probe: 3'FAM- CTG ATA GGC ACT GTT GAC - MGB 5'; RP: 3' GGG TAG TTG GGC AGT GAG AC 5') (Accession numbers: [GenBank:NM_001135 .2] (variant 1) and [GenBank:NM_01 322 7 .2] (variant 2) primers ... regenerating the degenerate disc Previous studies investigating the effect of CDMP1 on rabbit disc cells in monolayer [9] andmouse [22 ] and bovine disc cells in alginate [21 ] have shown significant increases ... resulted in significant increases in GAG production [21 ,22 ] The accumulation of GAG within alginate beads was investigated within the compartments: CM and FRM, together with GAG released into media...
... Le Grice SF (1995) Footprint analysis of FEBS Journal 2 76 (20 09) 15 06 15 16 ª 20 09 The Authors Journal compilation ª 20 09 FEBS J J Champoux and S J Schultz 57 58 59 60 61 62 63 64 65 66 67 68 69 ... Journal 2 76 (20 09) 15 06 15 16 ª 20 09 The Authors Journal compilation ª 20 09 FEBS J J Champoux and S J Schultz Rausch JW & Le Grice SF (20 04) ‘Binding, bending and bonding’: polypurine tract-primed initiation ... that is derived by proteolysis of p 66 andis missing the C-terminal RNase H domain (Fig 2) The p51 subunit is enzymatically inactive and simply plays a structural role in the protein The avian sarcoma-leukosis...
... Sense 5'GGTGAAGGTCGGAGTCAACG3', Antisense 5'CAAGTTGTCATGGATGACC3' Discussion Our results indicate that there is no correlation between CD4 and CD8 mRNA levels and numbers of cells expressing these ... virus type glycoprotein precursor retains a CD4-p56lck complex in the endoplasmic reticulum J Virol 19 92, 66 (4) :22 96- 23 01 Geleziunas R, Bour S, Boulerice F, Hiscott J and Wainberg MA Diminution ... template in a 25 µl PCR The remainder of the reactions were made up by µl PCR Buffer (Gibco BRL, Grand Island, NY), 0 .25 g of each primer, mM MgC 12 in each CD4 reaction and mM MgC 12 for CD8...
... 5'-CCTCGGTTTCCCAAAGCAGAA-3'; late RT forward, 5'-AAGCAAGTGTGTGTTCCCATCT-3'; late RT reverse, 5'-CACTTACCTGCAACCGGAGG-3'; integrated forward, 5'-GCTGCCGATTGGGATTTACAAC3'; integrated reverse, 5'-AATGTCTGATCCTCTTGGCTCTC-3' ... SIVsmE 660 replication was defined by TCID50, and SIVsmE543-GFP infection was defined as % GFP+ PBMC following infection with VSV -G pseudotyped SIVsmE 660 -GFP determined using flow cytometric analysis ... as participated in all assays SL participated in B-LCL phenotyping and staging assay TS participated in B-LCL phenotyping, staging, and fusion assay TC participated in staging and fusion assay...
... (5'-AAATCCTGTGTTACCCGCTC-3'), P2 (5'-TGGTGGCTCTGCAATATGAA3'), and P3 (5'-AATGAATGGATGCCTGGGGTTT-3') were used as the primer specific for CDV species, wildtype strains, and vaccine strain Onderstepoort, ... (AF478544), Dog Turkey (AY09 367 4), 5804-Han90 (X85000), 007LMT(AB2 127 29), Dog5B (AY297453), Dog26D (AB040 766 ), Dog98-0 02 (AB 025 270), DogHM-3 (AB040 767 ), Greenland (Z47 760 ), Liu (AF1 724 11), Onderstepoort ... Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China and 2Qingdao Agricultural University, Qingdao 26 6 109, China Received: 28 January 20 10 Accepted: May 20 10 Published: May 20 10...
... Medicine, Pittsburgh, PA 15 26 1 , USA and 2Department of Medicine, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15 26 1 , USA Received: 22 February 20 10 ... HIV-1 replication [23 - 26 ] and that the expression of many inflammatory cytokines, including TNF-α, is tightly regulated at the post-transcriptional level by HuR [27 ] Interestingly, several studies ... Microbe 20 08, 4:495-504 32 Ahn J, Byeon IJ, Byeon CH, Gronenborn AM: Insight into the structural basis of pro- and antiapoptotic p53 modulation by ASPP proteins J Biol Chem 20 09, 28 4:138 12- 13 822 33...
... Clone M36m3-1 Wild-type 5’ LTR Eae I Eae I BLV gag BLV s1 U3 68 cggcca U5 gccggt R 88 gag 67 9 PCR BLV s1 BLV gag U3 69 9 68 R cgAcca U5 gcTggt 88 gag 509 Eae I digestion PCR PCR 67 9 69 9 Eae I digestion ... PCR 63 2 pb 63 2 pb M 45 36 + 4538 4533 + - M 63 2 pb GG C TC C A A GGG C G T C T T G T432C T/C TC C A A GGG C G T C T/C C GG C T T G T 861 7C BLV provirus TG TCT T A C T T 3’ LTR C TG T T G TTT ... 20 08, 5: 16 http://www.retrovirology.com/content/5/1/ 16 B A 5 06 3 96 344 29 8 22 0 20 1 154 134 M -3 S +3 +50 +24 0 M M -3 S M4535 +3 +50 +24 0 M M45 36 D C 5 06 5 06 3 96 3 96 344 344 29 8 29 8 22 0 20 1 22 0...
... by Page 13 of 15 (page number not for citation purposes) Retrovirology 20 05, 2 : 62 using primers including 5'-Alu oligo (5'TCCCAGCTACTCGGGAGGCTGAGG-3') and 3'-LTR oligo (5'-AGGCAAGCTTTATTGAGGGCTTAAGC-3') ... amplifying IN1 -21 2 cDNA are IN- HindIII-ATG-5' andIN -21 2-XmaI3'(5'-CAATTCCCGGGTTTGTATGTCTGTTTGC-3) IN substitution mutants INKK215,9AA-YFP, INKK240,4AE-YFP and INRK 26 3 ,4AA-YFP, were generated by ... replaced an arginine and a lysine at positions of 26 3 and 26 4 by alanines inthis region and generated a mutant (INRK 26 3 ,4AA-YFP) The protein expression of different IN- YFP mutants in 29 3T cells...
... H.A Great Lakes Cladophora in the 21 st century: Same algae, different ecosystem J Great Lakes Res 20 10, 36( 2) , 24 8 -25 5 ISSN 20 76- 28 95 (Print), ISSN 20 76- 29 09 (Online) 20 13 International Energy ... depending on the season, chlorinated, dechlorinated, and then discharged Figure is a photograph of the algae growing in the launders The algae are a combination of several coexisting species including ... nitrogen dioxide (NO2), which often is greater Additionally, CO and hydrocarbons ISSN 20 76- 28 95 (Print), ISSN 20 76- 29 09 (Online) 20 13 International Energy & Environment Foundation All rights...
... SU9 U A 6 0. 564 140.8 SG9 G A 0.938 141.1 SU9 U A 0.894 109.3 SG9 G A 1.140 75.3 SU9 U A 0.535 73.9 SG9 G A 0. 865 97.3 SU9 U A 12 0. 26 3 76. 6 SG9 G A 13 0.447 85.4 35 .2 55.8 43.7 61 .0 35.0 63 .3 43.3 ... introduced by Ohtsuka and colleagues [29 ,30], are derived from the conventional hairpin ribozyme by dissecting the two domains at the hinge between helix and helix and rejoining helix to helix via a linker ... extended inactive conformation [34,35] Because of the single-stranded linker, FEBS Journal 27 2 (20 05) 4 464 –4474 ª 20 05 FEBS An engineered ribozyme for RNA sequence exchange joining the two domains in...
... feeling 33% 50% 17% 4% v.good good avg poor results first sight query answered interesting facts suprising facts 43% 65 % 62 % 66 % 38% 20 % 24 % 15% 20 % 15% 10% 13% 4% 6% overall feeling 54% 28 % 14% ... andis labeled with the corresponding PoS–tagged word group Node actions are used to trigger additional processing, e .g. , displaying the snippets, expanding the graph etc The nodes and edges ... G nter Neumann 20 06 Lanu guage Independent Answer Prediction from the Web In proceedings of the 5th FinTAL, Finland Eugenie Giesbrecht and Stefan Evert 20 09 Part-ofspeech tagging - a solved task?...
... converting linguistic resources Proceedings of LREC 20 02, Las Palmas, Spain, 20 02 A Przepiórkowski and M Wolinski The Unbearable Lightness of Tagging: A Case Study in Morphosyntactic Tagging of Polish ... Proceedings of ILT&CIP, 20 01 Figure 2: ExtraLink GUI The links in the right-hand window are generated after clicking on the marked named entity for Lisbon (marked in dark) The bottom left window ... languages covered by SProUT This involves the adaptation of the mapping into the ontology and a multilingual presentation of the ontology in the link target page D Petitpierre andG Russell MMORPH–the...
... text parsing systems This process is necessary for natural language parsing, because it is the first step in the parsing However, it is more laborious for Japanese and several other languages, which ... string in the morpheme dictionary and one sub-string of input text is repeated Thisis one to one comparison On the other hand, many to one comparison or one to many comparison is practicable in ... Unification Grammar", Proc l Oth International Joint Conference on Artificial Intelligence: 61 5 -61 8 Hamaguehl, S mad Suzuki, Y (1988) "Haxdwaxe-matchlng Algorithm for High Speed Linguistic Processing in...
... following pages, I’ll be guiding you through the ins and outs of cooking and eating according to Dr Rob’s glycemic-load diet, drawing on Dr Rob’s expertise of course The purpose of this cookbook is ... oz 12 oz 2 oz oz oz oz 51⁄3 oz oz oz oz oz oz oz 1¾ oz oz oz oz 3¼ oz oz 1¾ oz 5½ oz oz oz oz oz oz 2 oz oz Load 3 46 340 314 301 28 3 2 76 26 0 2 46 23 4 22 7 22 2 21 9 21 8 21 3 20 8 20 5 199 171 169 154 ... thisby preventing large amounts of glucose from rushing into your bloodstream all at once Eliminating these “glucose shocks” not only helps you lose weight while continuing to enjoy satisfying...