562 da in mice post delivery of claudin 5 sirna

In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles pptx

Ngày tải lên : 10/08/2014, 05:21

... PBS three Groups of BLAB/c mice were intraperitoneally injected with a single dose of 25 μg of LPS in 100 μl PBS After two hours, mice were introvenousely treated with 250 μg of JCV VLPs with ... http://www.jbiomedsci.com/content/17/1 /51 Page of Figure In vivo effects of IL-10 shRNA on production of IL-10 and TNF-α in BALB/c mice Groups of BALB/c mice were intraperitoneally injected with LPS ( 25 μg) After two hours, mice ... and the effects of cell apoptosis were determined by 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) staining (C) Cell viability was determined by trypan blue staining VLPs IL-10...

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Báo cáo y học: "Early Reversal of experimental colitis disease activity in mice following administration of an adenoviral IL-10 vector" pps

Ngày tải lên : 11/08/2014, 08:21

... Pearlstein GR, Jain A, Strober W: Antiinterleukin 12 treatment regulates apoptosis of Th1 T cells Page of 10 (page number not for citation purposes) Journal of Inflammation 20 05, 2:13 49 50 51 52 53 ... CD45RBhi CD4+ T cells Immunity 1994, 1 :55 3 -56 2 Powrie F, Leach MW, Mauze S, Caddle LB, Coffman RL: Phenotypically distinct subsets of CD4+ T cells induce or protect from chronic intestinal inflammation ... Pennline K, Clark DA, Miller MJ: Anti-inflammatory properties of interleukin-10 administration in hapten-induced colitis Eur J Pharmacol 1997, 323:2 45- 254 Oshima T, Pavlick K, Grisham MB, Jordan...

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Báo cáo y học: " Candida soluble cell wall β-glucan facilitates ovalbumin-induced allergic airway inflammation in mice: Possible role of antigen-presenting cells" ppt

Ngày tải lên : 12/08/2014, 14:20

... lungs Histological findings of hematoxylin and eosin (H&E)-stained Histological findings of hematoxylin and eosin (H&E)stained lungs The groups of mice were intratracheally inoculated with vehicle, ... homogenates Protein levels of cytokines and chemokines in the lung Protein levels of cytokines and chemokines in the lung homogenates The groups of mice were intratracheally inoculated with vehicle, ... manufacturer's instructions This technique is based on the incorporation of the pyrimidine analogue 5- bromo-2'-deoxyuridine (BrdU) instead of thymidine into the DNA of proliferating cells BrdU incorporated...

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Báo cáo y học: " Global gene expression patterns in the post-pneumonectomy lung of adult mice" ppt

Ngày tải lên : 12/08/2014, 14:20

... Wang W, El-Deiry WS: Insulin-like growth factor factor binding protein-2 is a novel mediator of p53 inhibition of insulin-like growth factor signaling Cancer Biol Ther 2006, 5: 1408-1414 Muller ... matrix in insulinlike growth factor (IGF) binding protein-2 regulation of IGFII action in normal human osteoblasts Growth Horm IGF Res 2003, 13:328-3 35 Arai T, Busby W Jr, Clemmons DR: Binding of insulin-like ... development at the end of gestation Endocrinology 2006, 147 :55 84 -55 91 Mariani TJ, Reed JJ, Shapiro SD: Expression profiling of the developing mouse lung: insights into the establishment of the extracellular...

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Báo cáo sinh học: "Comparison of different delivery systems of DNA vaccination for the induction of protection against tuberculosis in mice and guinea pigs" pps

Ngày tải lên : 14/08/2014, 19:22

... single subcutaneous injection of about 1 05 live bacteria in 50 μl saline Animals received a single-dose of an intramuscular injection of 2 .5 mg of microspheres in 50 μl saline into each quadriceps ... bacteria in 50 ml saline); Me-DNAhsp 65/ TDM-loaded microspheres (single intramuscular injection of DNAhsp 65 plus TDM-loaded PLGA 50 :50 microspheres); MePrime/boost (single intramuscular injection of ... 100% of DNAv plus TDM-loaded PLGA 50 :50 microspheres - 100% of DNAhsp 65 plus TDM-loaded PLGA 50 :50 microspheres A mixture of PLGA microspheres containing: - 50 % of DNAhsp 65 plus TDM-loaded PLGA 50 :50 ...

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Báo cáo y học: " Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice

Ngày tải lên : 02/11/2012, 10:19

... in 5- day period (Fig 1) † * Nor NS AP5 AP10 AP 25 Figure 250 .00 WBC no (x100/mm3) † † 200.00 150 .00 Nor NS AP5 AP10 100.00 AP 25 50.00 * * 0.00 day day day day 11 day 14 Figure Figure Effects of ... by a 45- minute incubation in peroxidase-labeled streptavidin (from DAKO kit) Finally the sections were stained with diaminobenzidine-H2O2 solution at room temperature The number of stained cells ... level on day and day 14 in NS group AP at all doses did not have any effect on peripheral WBC count in CY-treated mice on either day or day 11 However, the rate of recovery of WBC number in mice...

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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"

Ngày tải lên : 03/11/2012, 11:17

... expression of claudin- 1 in the cytoplasm, with some nuclear expression of claudin- 1 B) Treatment of these cells with PKA inhibitor results in nuclear translocation of claudin- 1 in as little as 15 minutes, ... [pS/pT]X[R/K] PKA PKC A568G_C569A_A570C_ A571G_C572A A589G_G590A_G591C A589G_G590A_G591C F :5' -gccccagtggaggatttacgcatatgccggcgaca-3' R :5' -tgtcgccggcatatgcgtaaatcctccactggggc-3' F :5' -ccagtgcaaagtctttgacgacttgctgaatctgagcagc-3' ... involved in claudin- 1 expression and localization 99 Indeed, a study shows that increasing β-catenin increases the expression of claudin- 1 in colorectal cancer cells, and that claudin- 1 expression...

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Textile and Apparel Barriers and Rules of Origin in a Post-ATC World

Ngày tải lên : 12/04/2013, 16:17

... 622N, in square meters) Change in unit value, a 2002 5 Tariff rate, b 20 05 ETE, 20 05 138.7 56 5.2 214.9 94.1 239.6 82.0 110.4 380.6 113.4 52 .5 5.3 -27.4 -59 .7 -22.0 -49.0 -9.7 -48 .5 -57 .5 -41.4 ... 15. 5 21.2 15. 4 16.3 11.7 9.3 29.2 22.6 6 .5 93.3 57 .6 33 .5 38.6 23.3 64.8 35. 3 34 .5 33.1 129.9 153 .7 260.4 141.0 129.4 127.6 854 .7 21.0 163.0 154 .9 62.2 11.1 26 .5 16.9 12 .5 23.9 94.2 16.8 41 .5 ... sectors in non-WTO countries that were subject to binding quotas in 20 05 Using a quota fill rate of 90 percent to indicate a binding quota, exports were restrained in 10 sectors from China, 10...

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Tài liệu Guidelines for the appointment of General Practitioners with Special Interests in the Delivery of Clinical Services pdf

Ngày tải lên : 14/02/2014, 21:20

... aims In principle ,the main activities of the service will be based around education and improving standards of sexual health medicine within the primary care organisation as well as offering ... within the PCO Good understanding of roles, responsibility and structure of PCOs and how to influence them to bring about improvement in delivery of sexual health services Understanding of primary ... evidence of taking part in multidisciplinary teamwork to plan and deliver service provision and individual patient care • Delivering multi- and uniprofessional training • Base line experience working...

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Tài liệu ‘Unheard voices’: listening to Refugees and Asylum seekers in the planning and delivery of mental health service provision in London. pptx

Ngày tải lên : 24/02/2014, 18:20

... about difficult things during this period User involvement User involvement was discussed both in terms of involvement in the planning and delivery of services and in terms of direct involvement or ... ongoing difficulties of individuals; for focusing too much on a limited range of reactions; for undermining traditional coping strategies; and for ignoring the role of culture in shaping meaning ... seclusion Interpretation and investigation of violent incidents Monitoring and investigating death in mental health services Reducing imprisonment and fear of mental health services Increased...

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Báo cáo khoa học: Efﬁcient and targeted delivery of siRNA in vivo pdf

Ngày tải lên : 06/03/2014, 01:23

... and intracellular barriers in siRNA delivery in vivo Regardless of administration routes, the final destination of siRNA is the cytoplasm of the target cell, where it incorporates into RNA-inducing ... half-life in vivo) [ 25 27] In addition, targeted delivery systems localize siRNA in the desired tissue, resulting in a reduction in the amount of siRNA required for efficient gene silencing in vivo, ... increase in inflammatory cytokines, including interferon-a and interleukin-12, in serum was observed when the low molecular mass protamine ⁄ siRNA complexes were administered, indicating negligible...

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Báo cáo khoa học: "Age Prediction in Blogs: A Study of Style, Content, and Online Behavior in Pre- and Post-Social Media Generations" ppt

Ngày tải lên : 07/03/2014, 22:20

... predicting age without using the ages of friends focuses on identifying important candidate features, including blogging characteristics (e.g., time of post) , text features (e.g., length of post) , ... of posts written in total Mode hour (00-23) and day the blogger posts Average number of comments per post number of emoticons1 number of internet acronyms1 number of words that are not found in ... 2009 The weka data mining software: An update Susan C Herring and John C Paolillo 2006 Gender and genre variation in weblogs Journal of Sociolinguistics, 10(4):439– 459 Susan C Herring, L.A Scheidt,...

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Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

Ngày tải lên : 16/03/2014, 23:20

... antibody staining Immunoreactivity is observable in recombinant protein (Rec), protein extracts from testis of mice at 20 and 30 dpp but absent in 10 dpp Coomassie blue staining of total protein shows ... biotinylated Stk33-fusion protein was affinity-purified using Soft LinkTM Soft Release Avidin Resin (Promega) following the manufacturer’s instructions Western blotting Recombinant Stk33-Protein ... 493 52 1 52 Hanks SK & Hunter T (19 95) Protein kinases The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification FASEB J 9, 57 6– 59 6 53 Hanks SK, Quinn AM...

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Báo cáo khoa học: Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans docx

Ngày tải lên : 16/03/2014, 23:20

... behavior of the thyroglobulin (670 kDa), c-globulin ( 158 kDa), ovalbumin (44 kDa), myoglobin (17 kDa) and vitamin B12 (1. 35 kDa) standards (Lower) Fractions containing standards and analyzed proteins ... important in determining the chain length of ubiquinone by replacing either component with homologs from other species and analyzing the type of ubiquinone produced FEBS Journal 272 (20 05) 56 06 56 22 ... (20 05) 56 06 56 22 ª 20 05 FEBS R Saiki et al Mammalian prenyl diphosphate synthases Fig (Continued) FEBS Journal 272 (20 05) 56 06 56 22 ª 20 05 FEBS 56 09 Mammalian prenyl diphosphate synthases investigate...

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Báo cáo Y học: Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice usingin vivo 13 C NMR measurements of hepatic carbohydrate metabolism docx

Ngày tải lên : 17/03/2014, 17:20

... start of glucose infusion For the calculation of glycogen C6 changes, data acquired within h of the start of the glucose infusion was not included to avoid any in uence of transient changes in the ... the increased rate of 13C label incorporation into the C6 position of glycogen in the ADM group is indicative of activated 6-phosphofructo-1-kinase, which can lead to increased 13C labeling at ... control (solid line) and ADM mouse (dashed line) 13C glucose concentration in the liver was maintained at about 10 lmolÆg)1 in both groups as shown in the top plot that into glycogen C1 in both groups,...

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Báo cáo khoa học: Disruption of the gene encoding 3b-hydroxysterol D14-reductase (Tm7sf2) in mice does not impair cholesterol biosynthesis pdf

Ngày tải lên : 23/03/2014, 06:20

... expressed sequence AI 451 557 1.60 1 .59 1 .59 1 .58 1 .58 1 .58 1 .57 1 .57 1 .53 1.62 1.62 1.61 1.60 1.60 1 .52 1 .52 1 .51 1 .50 1 .50 1 .50 Fold change determined by qRT-PCR cholesterol in microsomes from liver, ... Gfra1 1424118_a_at NM_0 255 65 Spc25b 1416299_at 1433 955 _at NM_011369 NM_1 451 25 Shcbp1b Brwd1b 1419319_at 1448314_at 14 259 93_a_at 1416 757 _at NM_011316 NM_007 659 NM_01 355 9 NM_02 650 7 Saa4 Cdc2ab Hsp110 ... NM_0107 45 NM_0 157 31 NM_033373 – NM_008706 NM_001034 851 , NM_0 254 59 NM_2071 05 Ly86 Atp9a Krt23 Nav2 Nqo1 1810015C04Rik H2-Ab1 1417900_a_at 1 455 316_x_at 1 458 5 85_ at 1447643_x_at NM_013703 XM_9 158 04...

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Báo cáo khoa học: Benzo[a]pyrene impairs b-adrenergic stimulation of adipose tissue lipolysis and causes weight gain in mice A novel molecular mechanism of toxicity for a common food pollutant doc

Ngày tải lên : 30/03/2014, 11:20

... saline (s), or (B) 0 .5 mgÆkg)1 B[a]P for the indicated times prior to receiving injections of either saline or 0. 25 mgÆkg)1 epinephrine Forty-ﬁve minutes after injection of epinephrine or saline, ... doses of norepinephrine exerted a signiﬁcant inhibition on lipoysis (Fig 1C) The initial step involved in the epinephrine ⁄ norepinephrine-induced lipolytic cascade includes activation of the ... 14 15 16 17 Time (days) Fig Effect of withdrawal of B[a]P treatment on weight gain in C57Bl ⁄ 6J mice on normal chow diet Mice (20–22 g, 11 weeks of age) maintained on normal chow diet were injected...

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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx

Ngày tải lên : 18/06/2014, 15:20

... hMNCs: 0.9 25 ± 0.008, n = 15 hES-ECs: 0. 952 ± 0.0 25, n = P < 0. 05) The CBF ratio of the hES-MC-injected group (0.968 ± 0.023, n = P < 0. 05) increased significantly compared to that of the saline- or ... hES-EC+MC-injected group (17.8 ± 2 .5/ mm2: n = 5, P < 0. 05) significantly decreased compared to that of the saline-injected group (43 .5 ± 5. 4/mm2: n = 5) (Figure 6D, E) Assessment of recovery of impaired motor ... positive controls An initial 15- minute, 95 C hotstart was used, followed by cycles consisting of minute denaturation at 94°C, minute annealing, and minute extension at 72°C A 10minute extension was...

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báo cáo hóa học:" Regression of orthotopic neuroblastoma in mice by targeting the endothelial and tumor cell compartments" pdf

Ngày tải lên : 18/06/2014, 15:20

... frequent administration at 20 mg once a day for days in cycles of 28 days duration [19] In preclinical studies in mice, CHS 828 could reduce growth of s.c NB without any signs of toxicity [17] In order ... survival 85: 155 5- 155 9 Wassberg E, Stridsberg M, Christofferson R: Plasma levels of chromogranin A are directly proportional to tumour burden in neuroblastoma J Endocrinol 1996, 151 :2 25- 230 Seregni ... with intact plasma membrane (blue; DAPI staining) and cells with disrupted membrane (red; propidium iodine staining), magnification in A-B: × 100 CHS 828 induces regression of rapidly growing...

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Báo cáo hóa học: " Comparison of three rapamycin dosing schedules in A/J Tsc2+/- mice and improved survival with angiogenesis inhibitor or asparaginase treatment in mice with subcutaneous tuberous sclerosis related tumors" docx

Ngày tải lên : 18/06/2014, 16:20

... agent vincristine, combination vincristine plus rapamycin, single agent sunitinib, combination sunitinib plus rapamycin, single agent bevacizumab, and combination bevacizumab plus rapamycin As ... lesions of Woodrum et al Journal of Translational Medicine 2010, 8:14 http://www.translational-medicine.com/content/8/1/14 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 the kidney, brain, and ... Asparaginase * Asparaginase+Rapamycin * # 100 3000 Percent survival Tumor Volume (mm 3) 350 0 250 0 2000 150 0 1000 75 50 25 500 0 10 20 30 40 50 60 70 80 90 100 110 Days of Treatment * p < 0. 05 as compared...

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