4— expression of results
Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
... inhibition of proliferation of VSMC, which is mechanistically linked to a KLF4-induced enhancement of the expression of the tumour suppressor gene p53 [5] Because of the important roles of KLF4 ... with siRNA of KLF4 The relative values of all results were determined and expressed as mean ± standard error of the mean of three experiments in duplicate *P < 0.05 siRNA expression of KLF4 in ... that the promoter of SR-BI contained multiple putative KLF4 binding sites However, the direct effect of KLF4 on the expression of SR-BI remains unknown Here, the expression of KLF4 in response...
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Báo cáo khoa học: Silencing the expression of mitochondrial acyl-CoA thioesterase I and acyl-CoA synthetase 4 inhibits hormone-induced steroidogenesis potx
... interfering RNA (siRNA) or of ACS4 by siRNA results in the inhibition of steroid synthesis, an effect that is overcome by the addition of exogenous AA In summary, our results demonstrate a critical ... MTE-I expression confirms a previously expected role of MTE-I in the regulation of the rate-limiting step in steroidogenesis through the release of AA [12] Considering the crucial role of StAR ... AA regulates the expression of this protein in steroidogenic cells [10], we hypothesized a decrease in StAR expression after MTE-I silencing Thus, we examined the expression of StAR protein in...
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Báo cáo khoa học: Functional expression of Pseudomonas aeruginosa GDP-4-keto6-deoxy-D-mannose reductase which synthesizes GDP-rhamnose docx
... with MALDI-TOF MS and NMR Protein expression and analysis The GAL1 and GAL10 promoters of the pESC-LEU expression vector are repressed by dextrose and induced by galactose In the expression experiments, ... alignments and the trees RESULTS Cloning of P aeruginosa rmd into a S cerevisiae expression vector We have previously cloned the gmd and wcaG genes of E coli and the gmd and wbcJ genes of H pylori into ... a modi®cation of the weft sequence for water suppression [16] A total of 4096 transients were acquired with a spectral width of 6100 Hz For the DQF COSY spectrum [17], a total of 4000 ´ 256 complex...
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Báo cáo khoa học: Thyroid hormone induces the expression of 4-1BB and activation of caspases in a thyroid hormone receptor-dependent manner pptx
... levels of the expression of each cDNA and of the reliability (Present/Absent call) were calculated with software (AFFYMETRIX MICROARRAY SUITE ver.4.0) For comparisons of the expression level of each ... Proliferation of the cells was also impaired in the presence of T3, though the degree of the inhibition of proliferation by T3 was rather weak (Fig 5C) According to the results of DNA microarray ... proliferation of the cells and significantly increased the degree of DNA fragmentation in FRTL cells (Fig 6C,D) Thus, it appears that induction of the expression of 4-1BB and subsequent activation of caspases...
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Báo cáo hóa học: "IThe tumor microenvironment of colorectal cancer: stromal TLR-4 expression as a potential prognostic marker" potx
... value of the percentage of expression for each marker in any tumor compartment was used as cut-off Statistical differences between groups were evaluated by the Log-rank test Results Expression of ... of tumor cells [48] We observed a strong correlation between the increased expression of these inflammation markers and increasing tissue dysplasia up to malignancy Page of 16 of the length of ... Journal of Translational Medicine 2010, 8:112 http://www.translational-medicine.com/content/8/1/112 Page of 16 Figure Expression of HGF in human colon tissues 4a Different expression of HGF (present...
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báo cáo hóa học: " 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside (AICAR) attenuates the expression of LPS- and Aβ peptide-induced inflammatory mediators in astroglia" pptx
... of the involvement of SM-ceramide cascade-signaling in expression of iNOS and cytokines [12] These observed alterations of SM-Cer- and ROS-mediated signaling, with LPS/Aβ-induced expression of ... http://www.jneuroinflammation.com/content/2/1/21 of AMP for activation of AMPK [18] Recently, we reported anti-inflammatory properties of AICAR through activation of AMPK [19] in glial cells AICAR was found to inhibit expression of pro-inflammatory ... Figure shows the expression of iNOS, MnSOD and COX-2 in glial cells Aβ peptide upregulated the LPSmediated expression of MnSOD Aβ peptides (1–40) and (1–42) induced the expression of iNOS, COX-2,...
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báo cáo hóa học: " Differential aquaporin 4 expression during edema build-up and resolution phases of brain inflammation" doc
... regulation of AQP4 expression could be an important determinant of the overall water content based on its involvement in the resolution of edema There have been several reports of altered AQP4 expression ... 5’ to 3’ Location of amplicon Amplicon length Efficiency AQP4 Isoform 1: NM_012825.3 Sens: TTGGACCAATCATAGGCGC 770 to 788 Isoform 213 pb 98.2% 90 pb 102.0% 778 to 796 Isoform Isoform 2: NM_001142366.1 ... interpreted as the result of a modification of BBB permeability Our study focused on AQP4 because of its role in the resolution of interstitial edema We found that AQP4 expression was strongly...
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Báo cáo y học: " Decrease in expression of bone morphogenetic proteins 4 and 5 in synovial tissue of patients with osteoarthritis and rheumatoid arthritis" ppt
... of RA and ND expression values: all values of RA tissues were lower than the minimum level found in ND tissues In OA, expression values of of 12 synovial tissues were within the range of ND expression ... http://arthritis-research.com/content/8/3/R58 Figure Expression of TNF-α, IL-1β, stromelysin and collagenase I in synovial tissues Results are presented as percentage of GAPDH expression on a logatissues rithmic ... decreased expression of BMP-4 and BMP-5 in RA in comparison with ND Moreover, BMP-4 was also lower in synovial tissue of patients with RA than in those with OA There was no difference of BMP expression...
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Báo cáo y học: "Association of reduced heme oxygenase-1 with excessive Toll-like receptor 4 expression in peripheral blood mononuclear cells in Behçet''''s disease" potx
... (Y Kirino) The source of funding had no role in the writing of the report or the decision to publish the results TLR4 of forced HO-1 expression on monocytes Effect mRNA expression in peripheralToll-like ... suppresses HO-1 expression in human monocytes, leading to augmentation of inflammatory responses, and that clinical efficacy of anti-TNF therapy is associated with restoration of HO-1 expression in ... dilutions of the PCR amplified products The data on HO-1, TLR2, TLR4, and TNF-α were standardized to the expression of GAPDH in the same samples, using multiplex PCR technique Expression level of HO-1...
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Cyclic stretch enhances the expression of Toll-like Receptor 4 gene in cultured cardiomyocytes via p38 MAP kinase and NF-B pathway doc
... computer-controlled application of sinusoidal negative pressure with a peak level of ≅ 15 kPa at a frequency of Hz (60 cycles per min) for various periods of time To determine the roles of c-Jun N-terminal ... inhibitor of JNK SB203580 is a highly specific, cell permeable inhibitor of p38 kinase PD98059 is a specific and potent inhibitor of p42/p44 MAP kinase For evaluation of secreted TNF-a on TLR4 expression, ... increase of TLR4 by cyclic stretch As shown in Fig 4, addition of losartan (100 nM) before stretch significantly inhibited the increase of TLR4 protein expression induced by stretch Addition of losartan...
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Báo cáo y học: "Divergent expression of claudin -1, -3, -4, -5 and -7 in developing human lung" potx
... http://respiratory-research.com/content/11/1/59 Page of 10 Relative Expression of Claudin A 100 80 60 40 20 Relative Expression of Claudin B W eeks 25 20 15 10 C Relative Expression of Claudin 13 13 16 16 20 20 20 ... great individual variability in the expression of all claudins analysed Expression of claudin-1 RNA increases towards the end of gestation, whereas RNA expressions of claudin-3 and -4 are at their ... Identification of methods for use of formalin-fixed, paraffin-embedded tissue samples in RNA expression profiling Genomics 2009, 94:341-348 23 Livak KJ, Schmittgen TD: Analysis of relative gene expression...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... designing a mutation in the gene of GLP-1 (7-37) This mutation resulted in the substitution of glycolamine for alanine at the second site of GLP-1 (7-37) peptide A sequence of nucleotide including 45 ... significant Results Expression of N-terminal His-tagged gAd-GLP-1-A fusion protein and Western blot analysis The Escherichia coli host strain BL21 (DE3) cells transformed with the expression vector ... function of protein, we designed an enterokinase cleavage site at the 5’ terminus of the gene of the gAd-GLP-1-A fusion protein, which was used to remove the His-tag [9] In our study, most of the...
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Báo cáo y học: "Effects of Losartan on expression of connexins at the early stage of atherosclerosis in rabbits"
... (16) These results suggest that the expression of Cx40 mRNA induced by high-fat diet is independent of the role of Ang II Conflict of Interest The authors have declared that no conflict of interest ... Figure 5: mRNA expression of Cx40 (panel A) and Cx43 (panel B) in the rabbit iliac arteries of different groups by RT-PCR Top panels represented mRNA expression of Cx40 and Cx43 from each of the groups ... difference in the expression of Cx40 between losartan group and high-fat diet group (P>0.05) Figure and independently showed the protein expression of Cx43 and Cx40 in the iliac arteries of different...
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Báo cáo y học: "Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene"
... amount of ldh expression Percentage of increase/decrease expression was calculated by dividing treated cultures over control cultures (control = 100%) Values represent the mean and standard error of ... [7] The number of mature liver schizonts increased in the presence of mAbs anti-CSP of P yoelii and the authors suggested that enhancement of infection could be a consequence of an interaction ... was 3.07 +/- 0.73 (mean +/- SD)] Efficacy of invasion was calculated as the percentage of ring infected erythrocytes of test cultures over the percentage of ring infected erythrocytes in control...
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Báo cáo y học: "High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis
... Results The profile of IAPs expression in the cells producing HBV The change of IAPs expression in the HBV expressing cells was first investigated by the comparison of the gene expression profile ... unchanged The results suggest that the long time stimulation of HBV viral proteins indeed changes the expression of apoptosis profile of host cells, which probably results in the carcinogenesis of liver ... (Table 1) The strong intensity of the dots implies that the increase of the expression of cIAP2 and cIAP1 most likely are reliable In contrast, the gene expression of XIAP seemed to be 21 folds...
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Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"
... demonstrated no expression of hMSH2 gene in the tumoral cells [15] With regard to sporadic forms of tumors, reduced expression of hMSH2 have been reported in a distinct subset of oral and neck ... characterized by the lack of hMSH2 protein expression [15] Moreover, Lo Muzio et al [29] reported that 5% of the cases of oral squamous cell carcinoma show diminished expression of hMSH2 protein In ... errors, the inclusion of cases that had a known history of exposure to recognized carcinogens, and the report of cases of lichenoid dysplasia as malignant transformation of lichen planus Despite...
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Kết quả chọn tạo giống lúa lai hai dòng mới TH3-4 Result of breeding new two-line hybrid rice combination, TH3-4
... (Nguyễn Hữu Nghĩa dịch) HAU-JICA ERCB Project (2003), Experimental technique for Bacterial blight of rice, Hanoi, 42p Phạm Chí Thành (1986), Phơng pháp thí nghiệm đồng ruộng (Giáo trình Đại học),...
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Influence of Polyferric Sulfate Coagulant on the amoA mRNA Expression of Ammonia Oxidizer in Activated Sludge
... each species of ammonia oxidizer was demonstrated by T-RFLP analysis of cDNA of amoA mRNA The profiles of T-RFLP in Run are shown in Fig Table shows the species of ammonia oxidizer of the major ... activity of ammonia oxidation Amount of amoA mRNA Number of AOB (×107 cells mL-1) (×106 Copies PCR-reaction-1) Community analysis of ammonia oxidizer based on amoA mRNA The difference of the effect of ... However, in the case of Fe/P molar ratio of 5, copy number of amoA mRNA obviously decreased and 40% of ammonia remained at the end of the batch experiment According to the result of T-RFLP analysis,...
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