4mechanisms of peptide induced tolerance
Báo cáo khoa học: Secondary structure conversions of Alzheimer’s Ab(1–40) peptide induced by membrane-mimicking detergents pdf
... b-sheet structures would be induced by peptides interacting with other peptides In this model, the complete loss of structure and higher mobility of the C-terminus of the peptide may be due to its ... Detergent -induced Ab(140) secondary structures Fig Circular dichroism spectra of 75 lM Ab(140) peptide in 10 mM phosphate buffer at pH 7.2 in the presence of different concentrations of detergent ... follow the structural transitions of the Ab(140) peptide (75 lm) induced by increasing concentrations of the membrane-mimicking detergent LiDS The 1H-15N HSQC spectrum of uniformly 15N-labeled Ab(140)...
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báo cáo hóa học: " 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside (AICAR) attenuates the expression of LPS- and Aβ peptide-induced inflammatory mediators in astroglia" pptx
... time of addition of stress stimuli Preparation of aged Aβ (1–40), (1–42) and (25–35) and induction of cells with β-amyloid peptide The Aβ peptides (25–35), (1–40), (1–42) and the reverse peptide ... shows the expression of iNOS, MnSOD and COX-2 in glial cells Aβ peptide upregulated the LPSmediated expression of MnSOD Aβ peptides (1–40) and (1–42) induced the expression of iNOS, COX-2, and ... of the involvement of SM-ceramide cascade-signaling in expression of iNOS and cytokines [12] These observed alterations of SM-Cer- and ROS-mediated signaling, with LPS/Aβ -induced expression of...
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báo cáo hóa học: " Participation of MCP-induced protein 1 in lipopolysaccharide preconditioning-induced ischemic stroke tolerance by regulating the expression of proinflammatory cytokines" pdf
... Participation of MCP -induced protein in lipopolysaccharide preconditioninginduced ischemic stroke tolerance by regulating the expression of proinflammatory cytokines Jian ... mechanisms of LPS -induced ischemic brain tolerance Methods: Transcription and expression of MCPIP1 gene was monitored by qRT-PCR and Western blot Mouse microglia was prepared from cortices of C57BL/6 ... studied whether there is loss of LPS preconditioning -induced ischemic stroke tolerance in MCPIP1 knockout mice and whether such effects involve regulation of expression of proinflammatory cytokines...
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báo cáo khoa học: " Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets" ppsx
... situation of sucrose -induced tolerance was characterised by the lifting of atrazine repression, in the case of APX1 and sAPX, or by the induction by sucrose-atrazine combination, in the case of http://www.biomedcentral.com/1471-2229/9/28 ... carried out on the adaptation of organisms to fluctuations of ROS combinations Mechanisms of sucrose -induced tolerance to singlet oxygen In contrast with non-induction of H2O2-scavenging systems, ... metal cofactor (Fe, Mn, or Cu and Zn), in Arabidopsis thaliana [46] Transcriptome profiling was carried out after 24 hours of treatment [18] Measurements of enzyme activities at different times of...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... expression profiles of proteins involved in the mitochondrial (bcl-2, bax, and bcl-x, etc.) and stress reaction-related pathways of apoptosis (Fig 2) Table Altered protein expression of JAR cells induced ... importance of Fas and FasL [15], and that of TNF-R1 and TNF-α[16], in preeclampsia Thus, hypoxia -induced changes in the expression of both proapoptotic and antiapoptotic proteins, and 43 the balance of ... transfection of Bag-1 Morpho/AS to JAR cells did not alter the expression of PTEN after the exposure to hypoxia, which would mean that the expression of PTEN, and also that of Bcl-X, was independent of...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... (CLP), the hepatic mRNA level of CD163 was similar with that of the sham group, which markedly decreased to approximately 7.0% of that of the CLP group (Fig 1) Table Effect of GdCl3 on serum aminotransferase ... to the inhibition of CYP activity observed in the absence of changes in protein expression in rabbit hepatocytes [22] ROS indirectly reduced the activity of selected isoforms of CYP by inducing ... role of KCs in the regulation of CYP isoform activities and gene expression profiles, partly by investigating the inflammatory signaling pathway Sham CLP GdCl GdCl3 + CLP CD163 β-actin Fig Effect of...
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
... compilation ª 2008 FEBS 689 PI3K/Akt in PrPC -induced MDR J Liang et al Multidrug resistance (MDR) is one of the major causes of failure of chemotherapy of human malignancies Studies have shown that ... various steps in the cytotoxic activity of the drug, from a decrease of drug accumulation in the cell to abrogation of apoptosis induced by the chemical substance Often several different mechanisms ... reverse the PrPC -induced MDR effect, partly through decreasing transcriptional activation of P-gp A Discussion B Fig The inducible effect of PrPC on Akt (A) Western blot analysis of the cells transfected...
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Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt
... with a tolerance of 0.00001 for direct space sum cut-off Simulation is performed with shake (tolerance ¼ ˚ 0.0005 A) on the hydrogens [63], a 2fs integration time ˚ and a cut-off distance of A ... interaction of A6 with either the six-member ring of T7 or through the methyl group and ⁄ or O4 of T7 Thus, base stacking prevails at the AC(6–7) step (Fig 2A) of PDwt, and the AT(6–7) step of PDmut ... the fluctuations Complete loss of stacking interaction is seldom seen (A) of the amino group of C7 with A6 (Fig 4B) or through the overlap of the six-member ring of C7 with A6 (Fig 4C) Thus, unlike...
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Báo cáo khoa học: A novel mechanism of TGFb-induced actin reorganization mediated by Smad proteins and Rho GTPases docx
... adenoviral overexpression of Smad2 ⁄ 3, we studied the role of Smad proteins in regulation of the RhoA ⁄ B genes, activation of these small GTPases and the induction of actin reorganization Finally, ... significance of the TGFb -induced actin reorganization, we assessed the Smad -induced transcriptional regulation of a-SMA, and its expression and incorporation into the microfilamentous network of fibroblasts ... in the cell [38,39] Of interest was the periodic pattern of induction of RhoB gene transcription by TGFb As shown in Fig 1B, TGFb induced RhoB gene expression 2.6-fold at h of treatment in HepG2...
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Báo cáo khoa học: Template-assisted rational design of peptide inhibitors of furin using the lysine fragment of the mung bean trypsin inhibitor pptx
... inhibitory activity of the Lys domain was higher than that of the Arg domain, this study focused on the former The Lys domain consists of two peptide chains, which are composed of 26 and nine residues, ... confirmed the hydrolysis of the P1–P1¢ peptide bond (Fig 3) Numerous studies suggest that conformational rigidity in the reactive site loop region of a peptide ⁄ protein inhibitor of proteases is a ... a gift from R.S Fuller (University of Michigan Medical School) [20] Peptide synthesis Peptides were synthesized by solid-phase peptide synthesis using a 430A peptide synthesizer (Applied Biosystems)...
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Báo cáo khoa học: Fragile X-related protein FXR1 controls posttranscriptional suppression of lipopolysaccharide-induced tumour necrosis factor-a production by transforming growth factor-b1 doc
... lipopolysaccharide (LPS) -induced TNF-a protein production Furthermore, inhibition of FXR1 production can abolish the suppression of TNF-a TGF inhibits LPS induction of TNF via FXR1 protein production induced ... of TNF via FXR1 production (Fig 4C) These findings show that TGFb1 -induced inhibition of TNF-a protein production is reversed when FXR1 is inhibited B Overexpression of FXR1 can suppress LPS -induced ... mL tube: 20 lL of Optimem, lg of each plasmid, and lL of Lipofectamine LTX (Invitrogen) and incubated at room temperature for 30 This mixture was then added slowly to cells in mL of Optimem The...
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Báo cáo khoa học: Fluorescence and FTIR study of pressure-induced structural modifications of horse liver alcohol dehydrogenase (HLADH) potx
... studies of pressureinduced structural changes of proteins, the first approach cannot be easily used because of the need of a data set of reference proteins as a function of pressure In pressureinduced ... explain the decrease of CSM for this range of pressure and hence the increase of polarity of the environment of Trp314 Moreover, pressure could also induce a reduction of size of internal cavities, ... any modifications of the enzyme’s intrinsic fluorescence would be a proof of pressure -induced unfolding, denaturation and/or dissociation of the dimeric enzyme However, because of technical limitations,...
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Báo cáo Y học: Regulation of peptide-chain elongation in mammalian cells pptx
... structure of the catalytic domain of one member of the a-kinase family (ChaK, a TRP channel) was recently analysed [45] This revealed surprising similarities to the overall fold of members of the ... availability of metabolic energy The potential physiological significance of these observations is clear: under conditions of modest energy deficit, activation of AMPK leads to increased phosphorylation of ... mutation of Trp84 Ó FEBS 2002 Control of translation elongation (Eur J Biochem 269) 5363 REGULATION OF eEF2 KINASE BY PKA Fig Schematic depiction of the structure of human eEF2 kinase The known in...
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Báo cáo Y học: Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge pptx
... engaged in the formation of four internal disulfide bridges in the native peptide The measured mass of the native peptide agreed with the calculated mass of the 21-residue peptide with four disulfide ... exclusive of the polyA tail and contains an ORF of 347 bases with a coding capacity of 85 amino acids The amino acid sequence of the 21-residue peptide was found at the C terminus of the ORF ... cycle (Ct) values of the serially diluted cDNA from the liver of a challenged fish The level of hepcidin gene expression was determined by the formula: units of hepcidin cDNA/units of 18S cDNA · 100...
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Báo cáo khoa học: ERK and cell death: Mechanisms of ERK-induced cell death – apoptosis, autophagy and senescence doc
... with classical markers of autophagy, such as induction of LC3 and conversion of LC3-I to LC3-II [48,49,118], induction of beclin [48], induction of BNIP-3 [66] and activation of G-interacting protein ... independently of p53 [24,35,36,41] Together, these data suggest that upregulation of the tumor suppressor p53 may be an important mechanism of Ras ⁄ Raf ⁄ ERK -induced apoptosis Other mediators of Ras ... mesenchyme by FGF8 -induced DUSP6 activity When activated by the expression of constitutively active MEK1, downregulation of DUSP6 or by the expression of a phosphataseinactive mutant of DUSP6 (C294S),...
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Báo cáo khoa học: Inhibitory activity of double-sequence analogues of trypsin inhibitor SFTI-1 from sunflower seeds: an example of peptide splicing docx
... example of peptide splicing A Łegowska et al ˛ A B Fig MS spectra and results of HPLC analysis of (A) [FK]BiSFTI-1 (8) and (B) a mixture of b-trypsin and [FK]BiSFTI-1: peak 2, analogue without tripeptide ... complex of SFTI-1 with trypsin However, its formation was significantly slower compared to that of a mixture of with trypsin In a mixture of with trypsin, only trace amounts of a complex of [Phe5]SFTI-1 ... compatible with the inhibitory activity of the peptides (Table 1), with all of them being potent inhibitors of the target enzymes Proteolytic susceptibility of the inhibitors was found to be in...
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benoiton - chemistry of peptide synthesis (crc, 2006)
... synthesis of peptides Part The use of anhydrides of N-acylated amino acids and derivatives of inorganic acids Ann Chem 572, 190, 1951 17 J Meienhofer The mixed carbonic anhydride method of peptide ... Fundamentals of Peptide Synthesis In practice, a peptide is formed by the combination of two amino acids joined together by the reaction of the carboxyl group of one amino acid with the amino group of ... synthesis of enantiomerically pure peptides Peptides are always synthesized starting from the carboxy-terminal residue 20 Chemistry of Peptide Synthesis chiral amino acid, so activation of a peptide...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
... Phenotypic profiles of DC precursor CD14+ monocytes A Phenotypic profiles of DC precursor CD14+ monocytes Illustrating the absence of DC markers on this monocyte population B DC phenotypic profiles: ... melanoma specific peptide epitopes [43] The use of hTERT peptides allowed this approach to be used with a wide range of tumour types, as hTERT peptides are expressed on >85% of cancers [55-57] ... no peptide, as illustrated in Figure 9B This was a surrogate measure of in vitro cytotoxicity against naturally processed peptides of hTERT, as SCC-4 is known to inherently express hTERT peptides...
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Báo cáo hóa học: " Prevention of hyperglycemia-induced myocardial apoptosis by gene silencing of Toll-like receptor-4" potx
... representative of experiments Page of Figure Inhibition of caspase-3 after gene silencing of TLR4 (A) Suppression of Fas expression in the hearts of STZ mice treated with TLR4 siRNA Diabetes was induced ... Figure Suppression of TLR4 and prevention of apoptosis by gene silencing of TLR4 (A) Suppression of TLR4 expression in the heart of STZ mice treated with TLR4 siRNA Diabetes was induced by STZ injection ... hearts of STZ mice (A) TLR4 expression in the hearts of STZ mice Injection of STZ induced Type I diabetes as described in Materials and Methods Control mice were injected with the same volume of...
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Báo cáo sinh học: " Kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line" docx
... cell surface of epithelial cells Confocal laser scanning image of the distribution of viral glycConfocal laser scanning image of the distribution of viral glycoproteins on cell surface of epithelial ... effect of the presence of RSV-specific IgG on infected epithelial cells over a period of time (1 hour) and on the viability of these cells Here, we present evidence that anti-RSV IgG induced ... Antibodyinduced modulation of rhabdovirus infection of neurons in vitro J Neuropathol Exp Neurol 1980, 39(5):507-22 Chesebro B, Wehrly K, Doig D, Nishio J: Antibody -induced modulation of Friend...
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