4epigenetics affecting neural cell fate
Effects of high glucose concentrations on the expression of genes involved in proliferation and cell fate specification of mouse embryonic neural stem cells
Ngày tải lên :
30/09/2015, 06:36
... involved in proliferation and cell fate specification of embryonic neural stem cells Society for Neuroscience 35th Annual Meeting, Abstract Number 24.10 (24 Neural Stem Cells in Brain Injury and ... High glucose alters the expression of genes involved in proliferation and cell- fate specification of embryonic neural stem cells Diabetologia (2006) 49: 1027-1038 J Fu, S S W Tay, E A Ling, S T ... stress in mouse neural stem cells J Neurochem (In revision) Conference Abstracts: J Fu, S S W Tay, E A Ling, S T Dheen (2004) Analysis of proliferation and differentiation of neural stem cells in a...
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Báo cáo khoa học: Tec family kinases Itk and Rlk⁄ Txk in T lymphocytes: cross-regulation of cytokine production and T-cell fates pot
Ngày tải lên :
28/03/2014, 22:21
... of CD4+ T cells into distinct effector T cells These subsets include Th1, Th2 and Th17 cells, which produce different cytokines that drive distinct types of immune responses [10] Th1 cells express ... and the elimination of extracellular parasites, but which also provide help for humoral (B -cell) responses Th17 cells are a more recently identified subset of T-helper cells that secrete IL-17A, ... cytokines in differentiation of CD4+ cells have been extensively studied, CD4+ T cells also need to be activated through their T -cell receptors in order to become effector cells Although less appreciated,...
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REGULATION OF CHOP TRANSLATION IN RESPONSE TO eIF2 PHOSPHORYLATION AND ITS ROLE IN CELL FATE
Ngày tải lên :
24/08/2014, 12:32
... proteins in the islet β cells can trigger ER stress, inducing cell death and depletion of the β cell mass Deletion of which in Akita mice significantly alleviates depletion of the β cell mass, supporting ... of CHOP sensitizes cells to apoptosis in response to ER stress .88 21 Regulation of CHOP levels in response to stress and induced eIF2~P is critical for cell fate .96 ... CHOP-/- FRT recipient cell line 42 vii Construction of the WT-uORF-CHOP/ FRT or ΔuORF-CHOP/FRT reporters 44 Stable expression of CHOP in FRT cells 45 10 Cell survival assays ...
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Connecting brain tumor stem cells with their primary tumor and exploring glycogen synthase kinase 3ß regulation of cell fate
Ngày tải lên :
03/10/2015, 21:57
... have shed light on the role of neural stem cells as the transformational cells in GBM formation, hence their accurate terminology as glioma stem cells or -initiating cells14, 16, 17 Traditionally, ... Cell Proliferation and Viability Assays To assess cellular proliferation rates, cells were harvested and plated into 96well plates at 5000 cells per well Wells containing media only without cells ... of the cell- of-origin alongside its differentiated progeny, as has been elegantly shown in intestinal cancers15 Recent works have highlighted the importance of neural stem cells as the cells-of-origin...
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Identification of novel cytosolic binding partners of the neural cell adhesion molecule NCAM and functional analysis of these interactions
Ngày tải lên :
25/11/2015, 13:27
... 36 3.3 Cell culture and immunofluorescence 36 3.3.1 PDL coating of glass coverslips for cell culture 36 3.3.2 CHO cells 37 3.3.2.1 Cell culture of CHO cells ... deleted intracellular domain Intracellular domain of human NCAM isoform 140 Human NCAM isoform 180 Intracellular domain of human NCAM isoform 180 Extracellular domain of human NCAM Intracellular ... 2-(N-Morpholino)-Propansulfonsọure Muscle specific domains Neural cadherin Neural cell adhesion molecule Intracellular domain of NCAM Nuclear factor-kappaB Neuron-glia cell adhesion molecule Nickel-nitrilotriacetic...
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Trends in Cell Signaling Pathways in Neuronal Fate Decision Edited by Sabine Wislet-Gendebien potx
Ngày tải lên :
16/03/2014, 20:21
... Epigenetic Regulation of Neural Differentiation from Embryonic Stem Cells 305 Atsushi Shimomura and Eri Hashino Chapter 13 Neural Fate of Mesenchymal Stem Cells and Neural Crest Stem Cells: Which Ways ... Injury-Induced Fate Decisions of Adult Neural Precursor Cells: Focus on SOCS2 and Related Signalling Pathways 241 Harleen S Basrai, Kimberly J Christie and Ann M Turnley Chapter 11 Neural Stem/Progenitor Cells ... 10 Trends in Cell Signaling Pathways in Neuronal Fate Decision and ependymal cells [95] All three of these receptors are expressed in type A cells of the SVZ, while type B and C cells express...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
Ngày tải lên :
18/06/2014, 16:20
... administration or neural stem cell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stem cell transplant ... neurotrophins in the lesioned micro-environment of the CNS Materials and methods Culture of Neural stem cells Neural stem cells were harvested from the cortex of E16 Sprague-Dawley rat embryos The head was ... SP, Tsang KS, Chan AB, Lu G, Poon WS, Li K, Baum LW, Ng HK: Trophism of neural progenitor cells to embryonic stem cells: neural induction and transplantation in a mouse ischemic stroke model J...
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Báo cáo y học: " A cell surface interaction network of neural leucine-rich repeat receptor" doc
Ngày tải lên :
09/08/2014, 20:20
... pair is expressed during neural development, we could construct dynamic maps of the neural intercellular recognition program Using a recombinant protein library of 150 neural receptor ectodomains ... large families of neurally expressed receptors, there are several other families of cell surface proteins that contribute to neural recognition processes A comprehensive extracellular network ... motility of migrating cells or growth cones when many receptor proteins arrayed on apposing cell membranes interact These properties, however, make identifying novel extracellular recognition...
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báo cáo khoa học: " All-trans retinoic acid inhibits KIT activity and induces apoptosis in gastrointestinal stromal tumor GIST-T1 cell line by affecting on the expression of survivin and Bax protein" pdf
Ngày tải lên :
10/08/2014, 10:20
... confluent cell monolayer, the cell on the edge of the newly created gap will move toward the opening to close the scratch until cell to cell contacts are established again In this study, GIST-T1 cells ... avoid its cytotoxicity Cell proliferation assays Cell proliferation was determined by trypan blue dye exclusion test Cells were seeded in 6-well plates at a density of × 105 cells/ml in the presence ... were from Cell Signaling Technology Japan (Tokyo, Japan) Anti-PARP antibody was from WAKO Chemicals (Osaka, Japan) Cell morphologic assessment Cells were plated at a density of × 105 cells/ml...
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báo cáo khoa học: "Uptake and fate of surface modified silica nanoparticles in head and neck squamous cell carcinoma" pps
Ngày tải lên :
11/08/2014, 00:23
... comparable to other cancer cells, e.g cervical adenocarcinoma cells [43], osteosarcoma cells [42], lung adenocarcinoma cells [37,41], and gastric and colon cancer cells [44] Despite this, nanoparticle ... commercial cell proliferation assay (Cell Proliferation ELISA, BrdU, chemiluminescent, Roche, Basel, Switzerland) For this experiment the cells were cultured in black Greiner-96-well plates (2000 cells/well, ... exited the cells In contrast, our data implicates that both internalized Ru@SiO -OH and Ru@SiO -NH remain within the cell and are apparently distributed between daughter cells at cell division...
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Development of new neural stem cell based tumor targeted gene therapy approaches
Ngày tải lên :
09/09/2015, 10:06
... highly proliferating cells (hematopoietic progenitors, epithelial cells and endothelial cells), all antigen-presenting cells (DCs, activated monocytes and activated B cells), CD8+ T cells and eosinophil ... 5-fluorocytosine XV CHAPTER INTRODUCTION 1.1 Neural stem cells Neural stem cells (NSCs) are a self-renewing and multipotent population that gives rise to the three major neural lineages (neurons, astrocytes ... syncytiosomes 1.2.1.1 Cell fusion Overexpression of FMGs in tumor cells leads to massive cell to cell fusion, formation of gigantic, multinucleated syncytia and subsequent cell death in 2-5 days...
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Investigations on rab31s role in EGFR trafficking and neural progenitor cell differentiation towards astroglia
Ngày tải lên :
09/09/2015, 11:17
... constructs 36 2.2 Antibodies 37 2.3 Cell culture and transfection 38 2.4 Primary mouse neural progenitor cell (NPC) culture 38 ii 2.5 Expression silencing ... Introduction: Astrocytic cells and neurogenesis in the brain 110 6.2 Results: Rab31 in the adult rodent brain 112 6.3 Results: Rab31 in neural progenitor cells 117 6.4 Chapter ... appeared to decrease the percentage of GFAP-positive cells obtained This suggests that Rab31 plays a role in the differentiation of neural progenitor cells of the brain This may be, in part, due to...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
Ngày tải lên :
09/09/2015, 17:54
... ectoderm (including neural lineages), mesoderm and the endoderm However, the efficient differentiation of pluripotent stem cells into specific neural cell types requires exposure of the cells to the ... of ES cells and the mouse PA6 stromal cells could induce efficient dopamine neuronal differentiation They described the neuralizing activity conferred by the stromal cells as “stromal cell- derived ... important for determining regional cell fate, and this is mediated by signaling between adjacent cells by cellsurface proteins, and by gradients of signaling molecules The neural tube becomes regionalized...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
Ngày tải lên :
09/09/2015, 17:54
... the neuronal lncRNAs altered cellular differentiation fate from a neurogenic to a gliogenic program, and suggests that the lncRNAs play a key role in neural cell fate specification 115 Figure ... Figure 7.5: Schematic representation of differentiation of ReN-VM neural stem cells following transfection of siRNAs Neural stem cells were plated one day prior to siRNA transfection Neuronal differentiation ... regulate gene expression and specify neural cell fate specification 118 Figure 7.10: Apart from lncRNA_N2, the other neuronal lncRNAs were nuclearlocalized Cellular RNA was separated into the...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
Ngày tải lên :
09/09/2015, 17:54
... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... transcription factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST ... upon RMST knockdown in ReN-VM neural stem cells (Figures 7.4, 7.5 and 8.7) Knockdown of RMST appeared to shut down the neurogenic program of neural stem cells, with the cells adopting a glia lineage...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
Ngày tải lên :
09/09/2015, 17:55
... The cell biology of neurogenesis Nat Rev Mol Cell Biol 6, 777-788 Gregg, C., and Weiss, S (2003) Generation of functional radial glial cells by embryonic and adult forebrain neural stem cells ... G., Socci, N.D., Tabar, V., and Studer, L (2008) Human ES cell- derived neural rosettes reveal a functionally distinct early neural stem cell stage Genes Dev 22, 152-165 Engelen, E., Akinci, U., ... embryonic stem cells Cell 113, 643-655 Chambers, S.M., Fasano, C.A., Papapetrou, E.P., Tomishima, M., Sadelain, M., and Studer, L (2009) Highly efficient neural conversion of human ES and iPS cells by...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
Ngày tải lên :
09/09/2015, 18:56
... pluripotent stem cell derived -neural stem cell human induced pluripotent stem cell derived -neural stem cell expressing CodA human induced pluripotent stem cell derived -neural stem cell expressing Fcy human ... cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem cell as a vehicle for cancer gene therapy 1.5.1 Stem cell based cancer gene ... stem cell Human embryonic stem cell- derived neural stem cell Herpes simplex virus thymidine kinase Interleukin Intraperitoneal human induced pluripotent stem cell human induced pluripotent stem cell...
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