3beta from rat brain
Báo cáo khoa học: Activation of PMCA by calmodulin or ethanol in plasma membrane vesicles from rat brain involves dissociation of the acetylated tubulin/PMCA complex pdf
... PMVs from rat brain (A) PMVs were analyzed by western blotting by staining on separate lanes with 5F10 Ig, antiPMCA4 Ig and 6-11B-1 Ig (Ac-tubulin) Another sample of the same membrane preparation ... to estimate the Ca2+ concentration in living cells and how the concentration varied following ethanol treatment We used CAD cells from a mouse brain tumor that proliferate in serum-containing medium, ... epifluorescence with a confocal Zeiss LSM microscope Isolation of brain plasma membrane vesicles Isolation of plasma membrane vesicles from rat brain was based on the method of Michaelis et al [22], and...
- 13
- 339
- 0
Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
... Figure 4: Concentration of NOxx, TASy and TBARSz in different regions of rat brain in anoxia and reperfusion x Concentration expressed as nanomol of NOx /g wet weight of tissue y Concentration expressed ... 4-5 after the exposure of rats to hypobaric condition, the rats became lethargic and motionless At this juncture, the rats were removed and killed by decapitation The brains were quickly removed ... type Brain Res Rev 2001; 35: 73-96 42 Serrano J, Encinas JM, Salas E, et al Hypobaric hypoxia modifies constitutive nitric oxide synthase activity and protein nitration in the rat cerebellum Brain...
- 8
- 622
- 0
Báo cáo Y học: Variations in receptor site-3 on rat brain and insect sodium channels highlighted by binding of a funnel-web spider d-atracotoxin pdf
... 1,10-phenantroline (1 mM) All membrane preparation steps were performed on ice Rat brain synaptosomes were prepared from adult albino Sprague-Dawley rats ( 300 g, laboratory bred), according to the method ... locust sodium channels rather than between rat brain and cockroach pipettes d-ACTX-Hv1a was obtained from adult male or female H versuta spiders while d-ACTX-Ar1 was obtained from adult male A robustus ... dissociation rate [15,26,39,40,44–47] Allosteric modulation of d-ACTX-Hv1a binding site on rat brain synaptosomes Fig Scatchard plots of 125I-labelled d-ACTX-Hv1a binding to rat brain synaptosomes...
- 11
- 538
- 0
Báo cáo khoa học: Purification and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes doc
... Biochem 270) Table Substrate specificity of NTPDase1 and NTPDase2 from porcine brain cortex synaptosomes Reactions were carried out for 20 in the presence of mM substrates and liberated orthophosphate ... activity were concentrated, dialyzed and separated on a Mono Q HR 5/5 column equilibrated with 20 mM Tris/HCl buffer pH 8.0 with 0.05% polydocanol (w/v) The proteins were eluted from the column with ... separation of synaptosomal NTPDases Chromatography on this carrier allowed not only a marked purification of both proteins but also separation of NTPDase1 from NTPDase2 The NTPDase2 was eluted from...
- 8
- 321
- 0
Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid pdf
... resulted in the amelioration of the above parameters to a considerable extent (Table 1) Identification of monosaccharides by GLC As a first step, laminin from rat kidneys from different experimental ... of total carbohydrate content in SFC and SFD groups, respectively Purification of 2AA-labeled oligosaccharides from different experimental groups The low yield of laminin from rat kidney cortex ... sugar, urine sugar, urine volume and glomerular filtration rate (GFR) in diabetic rats Values are the mean ± SEM of six controls and 10 diabetic rats SFC, starch-fed control; FFC, dietary fiber-fed...
- 13
- 336
- 0
Báo cáo khoa học: Abundance of intrinsic disorder in SV-IV, a multifunctional androgen-dependent protein secreted from rat seminal vesicle pot
... major protein from rat seminal vesicle secretion A potential marker for androgen action J Biol Chem 254, 383–390 10 Pan Y-CE & Li SSL (1982) Structure of secretory protein IV from rat seminal vesicles ... inserted into it This process is totally different from the non-specific massive cooperative binding of SDS to proteins at submicellar concentrations, and mimics the situation that SV-IV experiences ... both farand near-UV circular dichroism data from our laboratory [18,20,22], experimental evidence that regular structure is scarce in SV-IV comes from SDS-PAGE, which is routinely used to assess...
- 12
- 337
- 0
Báo cáo khoa học: Cleavage of focal adhesion proteins and PKCd during lovastatin-induced apoptosis in spontaneously immortalized rat brain neuroblasts ppt
... immortalized rat brain neuroblasts undergoing apoptosis Lovastatin effects were concentration dependent and completely prevented by simultaneous exposure of cells to exogenous mevalonate, demonstrating ... and that the fluorogenic peptide substrate Ac-DEVD-AMC is also a substrate for caspase-7 [59] suggests that the cleavage of PKCd induced by lovastatin in rat brain neuroblasts undergoing apoptosis ... (Transduction Laboratories, BD Bioscience Europe) : 1000 for 90 at room temperature, mouse monoclonal anti-p130Cas (Transduction Laboratories, BD Bioscience Europe) : 750 for 90 at room temperature, rabbit...
- 13
- 364
- 0
báo cáo hóa học: " Evaluation of 18F-nifene binding to a4b2 nicotinic receptors in the rat brain using microPET imaging" ppt
... from running brain extracts and were compared to the peak to the parent compound providing evidence that the primary species within the brain of the rat was 18F-nifene After sacrificing the rat, ... deviations; Ratio measured at 85-90 postinjection of 18F-nifene; bRatio measured in the 60-min summed ex vivo scan of the same rats; cRatios measured in sections after the ex vivo scans of the same rats ... http://www.ejnmmires.com/content/1/1/6 Page of B A D C COR E CB Brain Hemisphere Homogenate Extract F COR TH 18F-Nifene Standard TH CB Figure Blood and brain metabolite analysis in rats postadministration of intravenous 18F-nifene...
- 9
- 392
- 0
Báo cáo y học: " Upregulation of Bax and Bcl-2 following prenatal cocaine exposure induces apoptosis in fetal rat brain" doc
... Bax in the brains treated with cocaine The middle panel shows quantitative data obtained from five separate experiments The bottom panel shows the Bax-to-Bcl-2 ratio in fetal rat brain Data are ... apoptosis DNA ladders in fetal rat brains were examined as previously described using the apoptotic DNA ladder kit from Sigma [8, 9] DNA was extracted from the brain according to the 296 instruction ... in fetal brain weight and the ratio of brain/ body weight (g/g) between cocaine 30 mg/kg/day and 60 mg /kg/day groups (Table 1) Table Effects of maternal cocaine administration on fetal brain weights...
- 8
- 403
- 0
Báo cáo y học: "Immunohistochemical localization of mu opioid receptor in the marginal division with comparison to patches in the neostriatum of the rat brain" docx
... border of the globus pallidus (GP), in the brain of rats (Figure 5A) The MrD can be characterized from the criteria of the rat atlas [49], as well as from its special neuronal morphology (Figure ... a mu-opioid receptor from rat- brain Molecular Pharmacology 1993, 44(1):8-12 Fukuda K, Kato S, Mori K, Nishi M, Takeshima H: Primary structures and expression from cdnas of rat opioid receptor ... in vivo from the Atlas of the rat brain Our data indicate that the MrD is an ideal choice for the study of MOR in the neostriatum in vivo Conclusions We have for the first time demonstrated the...
- 9
- 370
- 0
Báo cáo sinh học: " Transduction of the rat brain by Bovine Herpesvirus 4" pdf
... post BoHV-4EGFPΔTK injection Rat RMS Rat RMS area days post BoHV-4EGFPΔTK injection EGFP expression, 20× epifluorescence each brain and fields of view for each slice The ratio between EGFP positive ... in the rat brain, as compared to the replication-competent behaviour of BoHV-4EGFPΔTK observed in glioma cells in vitro To characterize further EGFP expressing cells in the transduced rat brains, ... Interestingly, all rats inoculated with the virus showed no clinical signs The transduction capability of BoHV-4EGFPΔTK was analyzed through EGFP expression in serial rat brain section, at 4,...
- 4
- 249
- 0
Identification and segmentation of the central sulcus from human brain MR image
... getting the mask of the brain tissues and getting the boundary look-up table 26 Chapter Removal of the Skull and Other Non -Brain Tissues 4.1 Introduction The disconnection of brain from skull and other ... and segment the central sulcus (CS) from human brain magnetic resonance (MR) volumetric images automatically The CS is an important landmark in the human brain since it demarcates the primary ... modify tissue contrast, MRI technology is suitable for imaging the human brain 1.1.2 Human Brain The study of the human brain, especially the cortex, is challenging due to its highly complex,...
- 76
- 408
- 0
Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf
... from relaxation rate ratios of single residues, within the MF context, most likely arose from inclusion in the dataset of the relaxation rates with slow exchange contributions (namely those from ... not far from the value computed from relaxation rate ratios Local generalized order parameters and internal effective correlation times Besides the optimization of the molecular tumbling rate, ... {1H}–15N NOE values were calculated from the height ratio of the peaks of 2D correlation spectra obtained with and without proton saturation, whereas the cross-relaxation rates, RNOE, were calculated...
- 14
- 744
- 0
Tài liệu Báo cáo khoa học: Leptin protects H9c2 rat cardiomyocytes from H2O2-induced apoptosis docx
... the emission shift from green (530 nm) to red (590 nm) using 488 nm excitation Composite red and green fluorescence is shown Results are representative of those from three separate experiments H2O2-induced ... H2O2induced changes (Fig 3) Leptin pretreatment reduces cytochrome c release from mitochondria Release of cytochrome c from mitochondria is a critical step in progression of the intrinsic apoptotic ... above, leptin- or leptin receptor-deficient rodents display an increased rate of cardiac apoptosis The increase in apoptotic rate and mortality was abolished upon leptin injection in ob ⁄ ob mice...
- 9
- 445
- 0
Tài liệu Báo cáo khoa học: Changes in purine specificity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx
... during product isolation [16] Substrate conversion was limited to < 10% Values are means ± SEM from two to eight experiments mostly carried out with proteins from two individual expressions Acknowledgements ... exchange of this region switched the specificity of the cyaB1 tandem GAF domain from cAMP to cGMP, confirming the above considerations Next we swapped the anterior part, Ile250 to Gly267, in cyaB1 GAF ... replaced by the rPDE2 tandem GAF domains Sequences in bold indicate residues from rPDE2 N ¼ 2–8; usually recombinant protein from two expressions were used Basal activity is expressed as nmol cAMPÆmg)1Æmin)1...
- 10
- 468
- 0
Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt
... analyzed: forebrain (with anterior hippocampus), midbrain (including substantia nigra) and medulla oblongata with cerebellum Adh1 and Adh4 signals were absent in brain tissue from both rats and mice ... roles in brain ethanol metabolism, Ó FEBS 2003 ADH expression patterns in mammalian brains (Eur J Biochem 270) 1321 Fig Expression patterns of the different classes of ADH in the brains of rat and ... FEBS 2003 ADH expression patterns in mammalian brains (Eur J Biochem 270) 1323 Fig ADH3 expression in neurons from different regions of the rat brain: pyramidal cells of the cortex and in the...
- 11
- 603
- 0
Báo cáo khoa học: Characterization of membrane-bound prolyl endopeptidase from brain ppt
... oligopeptidase: mPOP from pig brain, which we now call mPOP For more than 20 years, it has been known that some PE activity it associated with membranes from almost all tissues and especially from brain [18–20] ... particulate POP from pig brain We have confirmed that a significant amount of PE activity can be measured in the particulate fraction from crude pig brain homogenates In our preparations, this activity ... is shown in yellow Modeled from file mmdbId:21074, for POP from porcine brain, from the NCBI’s Entrez Structure database, and handled by CN3D v 4.1 (NCBI) software temperature dependence between...
- 13
- 470
- 0
Báo cáo khoa học: Oxidative stress induces a reversible flux of cysteine from tissues to blood in vivo in the rat pptx
... cysteine that enters RBCs cannot derive solely from plasma stores We also monitored the concentrations of thiols and disulfides in blood withdrawn from rats at 75 after the beginning of diamide infusion ... concentration in other tissues Diamide, at 60 from the start of administration, evoked a significant decrease in the hepatic Cys level, followed by an increase in both liver and kidney (after h from ... by separate analysis of plasma and erythrocyte compartments The total cysteine concentration in the plasma compartment increased from 100 to 140 lm (Fig 2) during diamide administration In...
- 13
- 510
- 0
Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
... membranes from human brain control and AD brains (A) After BN/PAGE, PS1 complex from control (CT) or AD cortex was detected in 50 lg membrane protein at 360 kDa (B) Analysis of samples from AD ... complex analysis from human brain of late onset sporadic AD cases and from cases with early onset PS1 mutations We found that PS1 complex from cortex of sporadic late-onset AD cases or from pathogenic ... c-secretase substrate APP or APP C-terminal fragments with the complex, BN/PAGE blots of membranes from SY5Y, APPC100 transgenic mouse brain and APP over-expressing transgenic mouse brain (Tg2576)...
- 11
- 488
- 0
Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf
... assays (EMSA) nRNE-d refers to nuclear extract prepared from normal rat liver and rRNE-d refers to nuclear extract prepared from regenerating rat liver sacrificed at h after surgery (time after partial ... chosen regenerating rat liver following partial hepatectomy, as a source of proliferating tissue to mimic in vivo conditions Surgical removal of twothirds of the liver results in regeneration of ... orchestrated regenerative response, activating a cascade of cell signalling events necessary for cell cycle progression and proliferation of hepatocytes Because progression of liver proliferation...
- 11
- 438
- 0
Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25