2tolerance induction by mucosal immunotherapy
Báo cáo Y học: Early growth response-1 gene (Egr-1 ) promoter induction by ionizing radiation in U87 malignant glioma cells in vitro pot
... promoter can be induced by a single dose of Gy The data presented indicate that radiation induction of the Egr-1 promoter is at least in part mediated by protein factors secreted by U87 cells in response ... effectively and quickly activated by growth factors, such as EGF, bFGF and other serum compounds [5,30,31], mechanisms leading to induction by radiation are by far less well understood Most likely ... factorb1 autoinduction J Biol Chem 264, 7041±7045 Santiago, F.S., Lowe, H.C., Day, F.L., Chesterman, C.N & Khachigian, L.M (1999) Early growth response factor-1 induction by injury is triggered by release...
- 10
- 280
- 0
Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering the acrosome reaction pdf
... [estimated by the absorbance at 260 nm (A260)] The reaction was stopped by the addition of EDTA to a final concentration of mM and then cooled on ice The S1 fraction was obtained as the supernatant by ... the fractions were analyzed, by electrophoresis, on a 2% agarose gel, followed by staining with ethidium bromide The proteins precipitated from each 2.2-mL fraction, by adding 2.8 mL of 10% (w/w) ... acetonitrile is shown by a dotted line The relative absorbance at 229 nm (upper panel) and fluorescence (excitation, 330 nm, emission, 510 nm; lower panel) are shown by bold lines by sucrose density-gradient...
- 10
- 503
- 0
Báo cáo y học: "Inorganic pyrophosphate generation by transforming growth factor-beta-1 is mainly dependent on ANK induction by Ras/Raf-1/extracellular signal-regulated kinase pathways in chondrocytes" docx
... 6b), the basal expression of Ank was increased by 2fold and 1.5-fold, respectively In these conditions, the induction of Ank by TGF-β1 was increased by 3-fold (Figure 6a) and 4.3-fold, respectively ... Ank nor its induction by TGF-β1 was affected by overexpression of Smad in chondrocytes (Figure 7b) These data demonstrated that the Smad pathway did not play a major role in the induction of ... a possible regulatory role for calcium in the induction of Ank expression by TGF-β1 Some genes induced by TGF-β1 are partly or totally regulated by Smad proteins We demonstrated that, in our...
- 13
- 312
- 0
Báo cáo y học: " Heterotopic pregnancy following ovulation induction by Clomiphene and a healthy live birth: a case report" pdf
... contribute to HTP [3,7-9] In our patient, pregnancy also occurred in association with ovulation induction by CC Discussion The majority of HTP cases are diagnosed late Significant morbidity and occasional ... 2008, 2:390 http://www.jmedicalcasereports.com/content/2/1/390 was positive with a normal tracing by pulse Doppler (Figures and 2) 1500 mL of old blood and abundant clots and a ruptured middle left ... significant complication She was successfully delivered of a male infant at 37 weeks gestation by CS (due to spontaneous onset of Figure sonogram before site of the pelvis (arrows) operation...
- 5
- 203
- 0
Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
... determined by real-time PCR (B) Protein levels of KLF4 were determined by western blot (C) mRNA levels of hSR-BI were determined by real-time PCR (D) Protein levels of hSR-BI were determined by western ... and HDL3 led to an induction of KLF4 in mRNA and protein levels in PBMC and PMA-differentiated THP-1 macrophages, the increment level induced by HDL3 was much higher than that by HDL2 Therefore, ... PMA-differentiated THP-1 macrophages stimulated by HDL3 The specificity of the assay was verified by using mutant oligonucleotides, which failed to bind to KLF4, and by antibody competition Meanwhile, the...
- 9
- 516
- 0
Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
... Erk activation Activation of Erk by various extracellular signals is often preceded by Shc phosphorylation Accordingly, we examined Shc activation, as indicated by its tyrosine phosphorylation, ... as indicated, followed by western blotting for Shc, phospho-Erk and total Erk levels (C) Rescue by ectopic Shc isoform expression of Erk activation that was suppressed by down-regulation of endogenous ... Erk phosphorylation was completely suppressed by the Src-specific inhibitor CGP77675 as well as by the MEK1 inhibitor UO126 and partially attenuated by the PI3K inhibitor Wortmannin No effect was...
- 14
- 599
- 0
Tài liệu Báo cáo khoa học: The Ets transcription factor ESE-1 mediates induction of the COX-2 gene by LPS in monocytes doc
... of ESE-1 to diminish COX-2 promoter induction in response to LPS or IL-1 exposure Results COX-2 induction by pro-inflammatory stimuli correlates with ESE-1 induction Our previous data had indicated ... expression in response to LPS in RAW 264.7 cells by western blot revealed that the temporal pattern of COX-2 protein induction upon stimulation by LPS correlated with the expression pattern of ... activation of the COX-2 promoter by ESE-1 and by LPS To examine whether the Ets sites in the COX-2 promoter are responsive to ESE-1 and to determine whether LPS induction of the COX-2 promoter...
- 12
- 519
- 0
Tài liệu Báo cáo Y học: Induction of (2¢)5¢)oligoadenylate synthetase in the marine sponges Suberites domuncula and Geodia cydonium by the bacterial endotoxin lipopolysaccharide docx
... products were identified by thin-layer chromatography, immunologically and by high-performance liquid chromatography The biological activity of (2–5)A oligomers was verified by inhibition of the protein ... after synthesis with shrimp alkaline phosphatase and analysed by HPLC The reaction products were also analyzed by TLC, followed by autoradiography to determine the product Based on these data ... by the jak/STAT pathway and initiated by cytokines [28] At present, studies on the elucidation of this pathway in S domuncula are in progress in our group As a consequence of the activation/induction...
- 11
- 578
- 0
Báo cáo khoa học: Induction of PPARb and prostacyclin (PGI2) synthesis by Raf signaling: failure of PGI2 to activate PPARb potx
... 4-OHT addition, whereas no induction was seen for cPLA2 This finding was confirmed by northern blotting which showed a 10-fold induction of Cox-2 mRNA after h (Fig 2B and C) Induction was specific for ... (B) Analysis by northern blotting of PPARb induction in the presence of UO126 or actinomycin D Shown is a quantitative evaluation of a northern blot by PhosphorImaging detect any induction of ... dramatic ERK-dependent induction of Cox-2 transcription and to a modest increase in mPGES-1 and PGIS mRNA expression (Fig 2) Induction of Cox-2 by Ras-dependent signaling, inclu- Raf induction of PGI2...
- 10
- 434
- 0
Báo cáo khoa học: Induction of raft-like domains by a myristoylated NAP-22 peptide and its Tyr mutant potx
... Bodipy-TMR-PI(4,5)P2 by the MARCKS peptide has been shown to be caused by sequestering of the labeled lipid into domains [12] We show that the quenching of the Bodipy fluorescence by the Y11L-NAP-22-peptide ... solubility limit of cholesterol [17] Fig Quenching of the fluorescence emission by the NAP-22-peptide (dashed lines) or by the Y11L-NAP-22-peptide (solid lines) of Bodipy-TMR-PI(4,5)P2 LUVs composed ... is caused by the slow rate of interconversion of two forms of anhydrous cholesterol crystals [15] The polymorphic transition of anhydrous cholesterol crystals is most clearly seen by DSC in heating...
- 12
- 369
- 0
Báo cáo khoa học: Induction of translationally controlled tumor protein (TCTP) by transcriptional and post-transcriptional mechanisms pot
... promoter modified with TCTP UTRs The Luc UTRs have been replaced by TCTP mRNA 5¢- and 3¢-UTRs (5¢ ⁄ 3¢), by TCTP mRNA 5¢-UTR (5¢), or by TCTP mRNA 3¢UTR The TCTP 3¢-UTR but not the 5¢-UTR stimulated ... improved expression by hypoxia is partially controlled by UTRs of the appropriate mRNAs in interaction with RNA-BPs [26,27] In the case of TCTP mRNA, this view is supported by our RNA–protein ... luciferase mRNA were replaced by human TCTP mRNA UTRs The UTRs (5¢-UTR, 134 nucleotides, and 3¢-UTR, 257 nucleotides) [16] were amplified by PCR, and restriction sites were added by primer extension The...
- 9
- 374
- 0
Báo cáo khoa học: Activation of Stat5 and induction of a pregnancy-like mammary gland differentiation by eicosapentaenoic and docosapentaenoic omega-3 fatty acids docx
... antibody followed by western analysis The expression of phosphorylated Stat5 was determined by using a specific antiphosphorylated Stat5 antibody and normalized for total Stat5 expression Induction of ... gland differentiation This model indicates that induction of mammary differentiation by alternation of the n-6 ⁄ n-3 ratio is mediated in part by activation of Jak2 ⁄ Stat5 signaling pathway ... particularly DPA and EPA accumulated in the mammary gland, which is mediated by B-FABP MRG Increased n-3 PUFAs activates Stat5 by induction of Stat5 tyrosine phosphorylation, stimulates milk protein...
- 12
- 421
- 0
Báo cáo Y học: Induction of chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI ) gene expression is mediated by ETS factor binding sites doc
... was able to bind speci®cally (Fig 3D, lane 3) and could be supershifted by an Ets-1 speci®c antibody (lane 2), but not by an unrelated antibody (lane 13) Increasing amounts (10 and 100-fold molar ... 12, 1043±1053 Lim, F., Kraut, N., Framptom, J & Graf, T (1992) DNA binding by c-Ets-1, but not v-Ets, is repressed by an intramolecular mechanism EMBO J 11, 643±652 Jorcyk, C.L., Watson, D.K., ... using both PCR fragments with arti®cial restriction sites at the ends and DNA fragments obtained by restriction digestion The oligos used for point mutations in the three ETS response elements...
- 9
- 360
- 0
Báo cáo khoa học: Rapamycin inhibits lipopolysaccharide induction of granulocyte-colony stimulating factor and inducible nitric oxide synthase expression in macrophages by reducing the levels of octamer-binding factor-2 doc
... macrophages by blocking mTOR activity G-CSF functions as an anti-inflammatory cytokine by activating antibacterial defense by neutrophils and by reducing the release of proinflammatory mediators, thereby ... both PI3K and mTOR [26], we speculated that the induction of Oct-2 expression by LPS might occur through an mTORdependent pathway and be inhibited by the mTOR inhibitor, rapamycin In addition, ... expression of Oct-2 mRNA and Oct-2 protein in RAW264.7 macrophages, and the induction of Oct-2 protein by LPS is blocked by a PI3K or an Akt inhibitor (A) RAW264.7 cells were treated with 100 ngÆmL)1...
- 12
- 376
- 0
Báo cáo khoa học: Induction of PAI-1 expression by tumor necrosis factor a in endothelial cells is mediated by its responsive element located in the 4G/5G site ppt
... ligand-stimulated NF-jB activation can be blocked by antioxidants, it appears that the generation of ROS may be involved in the induction of PAI-1 expression by TNFa via the activation of NF-jB Results ... followed by incubation with TNFa (50 ngÆmL)1) and the released PAI-1 antigen was determined by ELISA Figure 1A shows that the treatment of endothelial cells with TNFa increased PAI-1 antigen by almost ... complex is retained in the cytoplasm by IjB proteins Activation of NF-jB involves its phosphorylation by IjB kinase (IKK) and subsequent degradation of IjB by 26S proteasome To inhibit NF-jB,...
- 11
- 393
- 0
báo cáo hóa học: " Induction of serine racemase expression and D-serine release from microglia by amyloid β-peptide" pdf
... http://www.jneuroinflammation.com/content/1/1/2 Figure Induction of serine racemase by Aβ Induction of serine racemase by Aβ Serine racemase protein was detected by western blot analysis of lysates of primary ... luciferase levels indicated an induction of the presumptive serine racemase promoter by both stimuli (Fig 4) Previous experiments demonstrated a release of glutamate by microglia activated with ... any artifacts of cell number or lysis (not shown) D-serine is produced primarily by conversion from L-serine by serine racemase This racemase is known to be expressed in protoplasmic astrocytes...
- 11
- 493
- 0
báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx
... appreciated by the NFκB response induced by TNFα alone shown in figure 6B) The results obtained in PC12p75NTR(-) would suggest that p75NTR is essential to mediate iNOS induction by the combined ... reported in Figure 4, inhibition of NOS activity by L-NAME did not affect NFκB activation by NGF/TNFα combined treatment (Figure 5D) NGF/TNFα-promoted iNOS induction requires the simultaneous presence ... NO-derived oxidative damage brought about by a synergistic induction of iNOS Understanding the molecular mechanisms mediating the synergistic NGF/TNFα-promoted induction of iNOS may thus provide novel...
- 13
- 447
- 0
Báo cáo hóa học: " Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses" pot
... from the cell surface by removing sialic acid receptors from producer cells [12,26] To estimate the concentration of Flu-VLPs, we determined their 'transduction titres' by adding serial dilutions ... calculated by using the formula: Titre = %inf × (105/100) × d, where "d" is the dilution factor of the viral supernatant and "%inf" is the percentage of GFP-positive cells as determined by FACS ... http://www.virologyj.com/content/3/1/70 served regions of HA2 [31] Conformational changes were verified by demonstration of a complete loss of infectivity by low pH-treated particles (data not shown) [28] About 108 particles...
- 7
- 279
- 0
báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
... suppressed via the low induction of Ptgs2 expression In this study, the Ptgs2 expression level was suppressed by U0126, suggesting that EGF-like factor was required for the induction of cumulus ... original RT reaction The amplified products were analyzed by electrophoresis on 2% agarose gels The intensity of the objective bands was quantified by densitometric scanning using a Gel-Pro Analyzer ... were stored at -80°C until use After denaturing by boiling for min, 10 ul of each samples containing equal amounts of protein (10 ug) was separated by SDS-PAGE on 10% polyacrylamide gel, then transferred...
- 9
- 388
- 0
Báo cáo hóa học: " The Fabrication of Nano-Particles in Aqueous Solution From Oxyfluoride Glass Ceramics by Thermal Induction and Corrosion Treatment" pdf
... nano-particles in aqueous solution were prepared by means of thermal induction to produce nano-particles in glass matrix and corroding the glass host by hydrofluoric acid The nanoparticles in aqueous ... nano-particles may be interpreted by assuming that the nano-particles are a b-PbF2 solid solution in which Pb2? ions with ionic radius of 0.129 nm are partially substituted by Cd2? ions whose ionic radius ... been researched by our groups [16, 17] And the Er3? ion can be promoted to the 4I11/2 state through ground state absorption (GSA) of laser photons, and then to the 2H11/2 state by use of the excited...
- 5
- 274
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008