... mapping data from Marek s disease virus and herpesvirus of turkeys: implication for herpesvirus classification J Gen Virol 1988, 69, 20 33 -20 42 Calnek BW Pathogenesis of Marek s disease virus infection ... Biggs PM The history and biology of Marek s disease virus Curr Top Microbiol Immunol 20 01, 25 5, 1 -24 Buckmaster AE, Scott SD, Sanderson MJ, Boursnell MEG, Ross LJN, Binns MM Gene sequence and ... (strain HPRS24) was passaged in CEF cells at least 30 times prior to use in this study Preparation of immune sera against MDV Antisera against MDV2 (HPRS24) and HVT (FC 126 ) were prepared by repeated...
... compared to the overexpression of EGFP, KIND2 or KIND2-1 (Fig 5B) These results suggest that KIND2 and KIND2-1, overexpressed in neurons, act as dominant-negative regulators of v-KIND and suppress ... using the QuikChange XL Site Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) Pull-down assay GST-fusion proteins were prepared and used for the pulldown assays, as described previously ... KIND2 L461A, L474A and L477A mutants failed to bind to CD2-1- 62 of MAP2, whereas the L482A and L485A mutants did bind to this module These results suggest thatthe Leu461, 474 and 477 residues...
... Britain, and for the losses and damages sustained by British subjects by reason ofthe capture of their vessels and merchandise taken within the limits and jurisdiction ofthe United States and brought ... Papers ofthe Presidents 42 upon this subject It is but justice to say that this isthe first instance of misbehavior of any ofthe British officers toward our vessels of war that has come to my ... the inadmissible pretension of a right to prescribe the qualifications which a minister fromthe United States should possess, andthat while France is asserting the existence of a disposition...
... is so great as to excite our gravest apprehensions and to demand our wisest and most resolute action This criminal was a professed anarchist, inflamed by the teachings of professed anarchists, ... preventatives of war Of course no business man will go into the Philippines unless it is to his interest to so; and it is immensely to the interest ofthe islands that he should go in It is therefore ... policy of all the nations ofthe two Compilation ofthe Messages and Papers ofthe Presidents 18 Americas, as it isofthe United States Just seventy-eight years have passed since President Monroe...
... [2Fe) 2S] clusters, FeSI and FeSII, became visible Their assignment is given in Fig 4A for the wild-type Qor reduced with quinoline In this case, the g2-component of FeSII is superimposed by the ... of qorMSL [44] Stratagene [67] [68] This work This work This work This work [ 42] [44] This work This work This work This work This work This work This work [ 52] This work KT2440 and P putida 86-1 ... excess of dithionite, the signals of both FeS-centers appeared in appreciable intensity as indicated in Fig 4B The absence of Mo(V)signals reveals the g2-component of FeSII It is noted that the...
... a settled basis in this island; and we may suppose that this settlement was made on equitable terms, since Aristotle was not aware of any insurrection ofthe slaves in Crete against their masters. (20 1) ... lance and sword and shield he reaped and dressed his vines, and hence was called lord ofthe Mnoia." (20 2) In this island, however, different classes of dependents must have existed Sosicrates and ... viz., one consisting ofthe Theræans and Perioeci, the second of Peloponnesians and Cretans, andthe third of all the islanders. (24 5) From this it is evident thatthe original colonists still continued...
... antibodies [51], is consistent with this hypothesis The GPI-microdomains, whichare rich in src-family kinases [13,14] may also promote/regulate assembly ofthe IL-2R subunits with these enzymes in case ... regardless ofthe expression level of b and cc chains or class I HLA (see MT-1 cells; Fig 1B) It is noteworthy that FRET between CD25 andthe light chain (b2-microglobulin) of class I HLA was consistently ... outside lipid rafts [34,35] in these T cells, as shown by previous [43] and present FRET data As class I and class II HLA molecules were also found partially associated with TrfRs on T cells [43],...
... expenses from Novo Nordisk SES is an employee of Novo Nordisk Authors' contributions SES participated in the conceptualisation and design ofthe original web-based survey FJS, SES and FP assessed ... as a result of insulin treatment is particularly an issue for those who already are overweight Conclusion The results from this study suggest thatthe ITAS is a valid self-report instrument that ... to the patient 's needs Also, the instrument has potential to assess changes in the appraisal of insulin over time, both in individuals and groups Future research should establish its test-retest...
... expenses from Novo Nordisk SES is an employee of Novo Nordisk Authors' contributions SES participated in the conceptualisation and design ofthe original web-based survey FJS, SES and FP assessed ... as a result of insulin treatment is particularly an issue for those who already are overweight Conclusion The results from this study suggest thatthe ITAS is a valid self-report instrument that ... to the patient 's needs Also, the instrument has potential to assess changes in the appraisal of insulin over time, both in individuals and groups Future research should establish its test-retest...
... the solution located at the right side, is a proof of compound diffusion fromthe donor liposomes, passing across the dialysis membrane and incorporation in the membrane ofthe acceptor liposomes ... in the study After the dialysis assay, the fluorescence of compound in the donor liposomes is notably reduced (Figure 3), and its emission can be detected in the acceptor liposomes solution, showing ... DA isthe donor emission intensity after the dialysis experiment in NBD-labelled lipid/DPPC/DPPG liposomes, ID i isthe initial donor emission intensity in DPPC/DPPG liposomes and ID f is the...
... biocatalysis at 0.5 mM substrate concentration Discussion The oxygenating subunit ofthe 2, 5-diketocamphane monooxygenase was successfully cloned and overexpressed recombinantly in E coli asthe heterologous ... BL21 The 2, 5-DKCMO-gene was introduced using the sites of restriction endonucleases NdeI and XhoI for cloning with the BCA-kit and a standard curve of BSA in the same buffer in a range of 2- 0.005 ... SDS-PAGE analysis, the supernatant was substituted with Laemmli buffer (Laemmli 1970) SDS-PAGE was carried out on 12% resolving gels Proteins were stained with a Coomassie R250/G250 solution Enzyme...
... The scientific basis for understanding Semmelweis s observations was to be established in the years that followed by Pasteur and Joseph Lister, as described below 3.5 .2 Joseph Lister Fig 3.6 A ... of acute wounds and chronic lesions andthe prevention of possible complications such as cellulitis, osteomyelitis, and sepsis 3.6 Investigation of Wound Healing Processes The establishment of ... Erythema elevatum diutinum Connective tissue/multisystem diseases Rheumatoid arthritis Systemic lupus erythematosus Dermatomyositis Polyarteritis nodosa Wegeners granulomatosis Churg-Strauss syndrome...
... analysis in the ear asthe basis for discrimination of complex sounds such as speech sounds and instead emphasizing the role ofthe temporal coding of sounds such as vowels and it is now believed that ... amplitude ofwhich increases with the increase in the sound pressure ofthe sound that elicits the CM Recording ofthe CM is often used to determine changes in sound transmission ofthe middle ear The ... is independent ofthe frequency ofthe sound The differences in the sound that reaches the two ears are processed and discriminated in the central nervous system (see p 143) The basis for discriminating...
... experiments to assess the replicative capacity of these viruses would be required to assess the impact of Q96H/C/Y substitutions in the genetic context of HIV2 Other polymorphisms previously described ... impact ofthe Q91R and I175M mutations on susceptibility to RAL was assessed in vitro using an MTT assay as described previously [ 32] As shown in Figure 2, the Q91R and I175M substitutions conferred ... phase II-III clinical trials, arguing against the hypothesis thatthe N155 H mutation emerged as a resistance mutation per se or that an INI-resistant strain was transmitted These facts are rather...
... in what iscalled his discourse against the sophists, reproaches the teachers of his own times with inconsistency ‘They make the most magnificent promises to their scholars,’ says he, and undertake ... not, I presume, suppose that it was as large asthe life His way of living, as well asthatof Hippias and Protagoras, two other eminent teachers of those times, is represented by Plato as splendid ... profits of stock must vary with it, must sink as it sinks, and rise as it rises The progress The Wealth of Nations 21 8 [5] 21 9 [6] 22 0 [7] 22 1 [8] Adam Smith of interest, therefore, may lead us to...
... compromise between faster response and smaller overshoot As an extension of these results, the input-output relationship of a class of nonsymmetric type -2 fuzzy PD and PI controllers was established ... Although the reasoning methods in the theory of fuzzy logic is not limited to these two, this thesis will focus on these two reasoning processes 1.1.1 Fuzzy control The application of fuzzy logic system ... converts the output ofthe inference engine into an interval set Figure 2. 10: The structure of type -2 fuzzy logic system 2. 3 .2 The Sup-star Composition Inference System Since the focus of this thesis...
... Business Models Summary and Conclusion 418 418 418 418 418 420 421 421 421 421 421 422 422 422 422 422 423 423 423 424 424 424 424 424 424 425 425 425 426 426 426 426 426 427 427 427 427 428 428 428 ... collected by wind measurements, thewind speed andthe frequency distribution during the coming 20 years have to be estimated This prognosis has to be based on solid assumptions If thewindis measured ... expected There is a risk and a chance To evaluate these is a matter for the investors andthe owner/operator To that, a well-founded risk-analysis is necessary Such risk assessment can be made by the...
... cystogenesis The association of AC and other diseases in families suggested that PAPB2, SPG4 and SOX2 gene might be linked to AC21 -23 Consequently, further sequencing of those candidate genes is ... counterpart ofthe symptomatic patient with AC as early as possible, irrespective ofthe absence of symptoms Conflict of Interest The authors have declared that no conflict of interest exists References ... in the predisposition to cystogenesis in some patients Previously, both the familial occurrence of AC andthe presence of AC in patients with genetically determined cystic diseases, such as ADPKD,...
... frontal sinuses [1] CT scans of agenetic patients show almost normal frontal recesses and sinuses, although there is only one frontal sinus ostium The size ofthe sinus and, therefore, its anatomic ... was analyzed using the weighted kappa test Results In the DVT scans, both agenesis (on the right) and aplasia (on the left) were seen in one case (0 .24 %; Fig 2) The results fromthe present study ... development ofthe frontal sinus [1,5,18 ,27 ] There is a direct relationship between the mechanical stresses of mastication and frontal sinus enlargement [8, 28 ,29 ] and if there is persistence of a metopic...
... processes secondary to the trauma may in some rare instances stimulate neoplastic proliferation; for these reasons it is important thatthe tumor arises at the site ofthe old scar to demonstrate ... relative risk may reflect the fact thatthe association, if it exists, is not a direct one The mechanism of post-traumatic brain cancer is unclear Experimental data have shown that trauma can act as ... trauma was not histologically verified; it must be emphasized, however, that CT scan documented the absence of tumor at the time ofthe brain injury We suggest that these conditions for the acceptance...